Three-Dimensional Reconstruction, by TEM Tomography, of the Ultrastructural Modifications Occurring in Cucumis sativus L. Mitochondria under Fe Deficiency
Mitochondria, as recently suggested, might be involved in iron sensing and signalling pathways in plant cells. For a better understanding of the role of these organelles in mediating the Fe deficiency responses in plant cells, it is crucial to provide a full overview of their modifications occurring...
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| description | Mitochondria, as recently suggested, might be involved in iron sensing and signalling pathways in plant cells. For a better understanding of the role of these organelles in mediating the Fe deficiency responses in plant cells, it is crucial to provide a full overview of their modifications occurring under Fe-limited conditions. The aim of this work is to characterize the ultrastructural as well as the biochemical changes occurring in leaf mitochondria of cucumber (Cucumis sativus L.) plants grown under Fe deficiency.
Mitochondrial ultrastructure was investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allowed a three-dimensional (3D) reconstruction of cellular structures. These analyses reveal that mitochondria isolated from cucumber leaves appear in the cristae junction model conformation and that Fe deficiency strongly alters both the number and the volume of cristae. The ultrastructural changes observed in mitochondria isolated from Fe-deficient leaves reflect a metabolic status characterized by a respiratory chain operating at a lower rate (orthodox-like conformation) with respect to mitochondria from control leaves.
To our knowledge, this is the first report showing a 3D reconstruction of plant mitochondria. Furthermore, these results suggest that a detailed characterization of the link between changes in the ultrastructure and functionality of mitochondria during different nutritional conditions, can provide a successful approach to understand the role of these organelles in the plant response to Fe deficiency. |
| doi_str_mv | 10.1371/journal.pone.0129141 |
| format | Article |
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Mitochondrial ultrastructure was investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allowed a three-dimensional (3D) reconstruction of cellular structures. These analyses reveal that mitochondria isolated from cucumber leaves appear in the cristae junction model conformation and that Fe deficiency strongly alters both the number and the volume of cristae. The ultrastructural changes observed in mitochondria isolated from Fe-deficient leaves reflect a metabolic status characterized by a respiratory chain operating at a lower rate (orthodox-like conformation) with respect to mitochondria from control leaves.
To our knowledge, this is the first report showing a 3D reconstruction of plant mitochondria. Furthermore, these results suggest that a detailed characterization of the link between changes in the ultrastructure and functionality of mitochondria during different nutritional conditions, can provide a successful approach to understand the role of these organelles in the plant response to Fe deficiency.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0129141</identifier><identifier>PMID: 26107946</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Apoptosis ; Cellular structure ; Chloroplasts ; Conformation ; Cristae ; Cucumis sativus ; Cucumis sativus - metabolism ; Cucumis sativus - ultrastructure ; Electron Microscope Tomography ; Electron microscopy ; Electron transport ; Electron Transport - physiology ; Homeostasis ; Iron ; Iron - deficiency ; Leaves ; Metabolism ; Mitochondria ; Mitochondria - metabolism ; Mitochondria - ultrastructure ; Mitochondrial DNA ; Organelles ; Plant cells ; Plant Leaves - metabolism ; Plant Leaves - ultrastructure ; Plant mitochondria ; Reconstruction ; Respiration ; Signal Transduction ; Tomography ; Transmission electron microscopy ; Ultrastructure</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0129141-e0129141</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Vigani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Vigani et al 2015 Vigani et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-40ab1d88c5c3eea4b88e9f7192ad81b1de34e7593feae432ca23cb59caae5ddd3</citedby><cites>FETCH-LOGICAL-c692t-40ab1d88c5c3eea4b88e9f7192ad81b1de34e7593feae432ca23cb59caae5ddd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479487/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479487/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26107946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vigani, Gianpiero</creatorcontrib><creatorcontrib>Faoro, Franco</creatorcontrib><creatorcontrib>Ferretti, Anna Maria</creatorcontrib><creatorcontrib>Cantele, Francesca</creatorcontrib><creatorcontrib>Maffi, Dario</creatorcontrib><creatorcontrib>Marelli, Marcello</creatorcontrib><creatorcontrib>Maver, Mauro</creatorcontrib><creatorcontrib>Murgia, Irene</creatorcontrib><creatorcontrib>Zocchi, Graziano</creatorcontrib><title>Three-Dimensional Reconstruction, by TEM Tomography, of the Ultrastructural Modifications Occurring in Cucumis sativus L. Mitochondria under Fe Deficiency</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mitochondria, as recently suggested, might be involved in iron sensing and signalling pathways in plant cells. For a better understanding of the role of these organelles in mediating the Fe deficiency responses in plant cells, it is crucial to provide a full overview of their modifications occurring under Fe-limited conditions. The aim of this work is to characterize the ultrastructural as well as the biochemical changes occurring in leaf mitochondria of cucumber (Cucumis sativus L.) plants grown under Fe deficiency.
Mitochondrial ultrastructure was investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allowed a three-dimensional (3D) reconstruction of cellular structures. These analyses reveal that mitochondria isolated from cucumber leaves appear in the cristae junction model conformation and that Fe deficiency strongly alters both the number and the volume of cristae. The ultrastructural changes observed in mitochondria isolated from Fe-deficient leaves reflect a metabolic status characterized by a respiratory chain operating at a lower rate (orthodox-like conformation) with respect to mitochondria from control leaves.
To our knowledge, this is the first report showing a 3D reconstruction of plant mitochondria. Furthermore, these results suggest that a detailed characterization of the link between changes in the ultrastructure and functionality of mitochondria during different nutritional conditions, can provide a successful approach to understand the role of these organelles in the plant response to Fe deficiency.</description><subject>Apoptosis</subject><subject>Cellular structure</subject><subject>Chloroplasts</subject><subject>Conformation</subject><subject>Cristae</subject><subject>Cucumis sativus</subject><subject>Cucumis sativus - metabolism</subject><subject>Cucumis sativus - ultrastructure</subject><subject>Electron Microscope Tomography</subject><subject>Electron microscopy</subject><subject>Electron transport</subject><subject>Electron Transport - physiology</subject><subject>Homeostasis</subject><subject>Iron</subject><subject>Iron - deficiency</subject><subject>Leaves</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - ultrastructure</subject><subject>Mitochondrial DNA</subject><subject>Organelles</subject><subject>Plant cells</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - ultrastructure</subject><subject>Plant mitochondria</subject><subject>Reconstruction</subject><subject>Respiration</subject><subject>Signal Transduction</subject><subject>Tomography</subject><subject>Transmission electron microscopy</subject><subject>Ultrastructure</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISGQ1hLH-ecbpKnboFKrSqPj1nLsk8ZVahc7nuir8LS4azY1aBcoF4nO-X3fic_xiaK3OB5jUuAva-Ot5u14azSMY5xQnOJn0SmmJBnlSUyeH32fRK-cW8dxRso8fxmdJDmOC5rmp9GfZWMBRpdqA9opEwzRDQijXWe96ELgHFU7tLyao6XZmJXl22Z3jkyNugbQbdtZfiC9Dcq5kapWgu91Di2E8NYqvUJKo4kXfqMcciF55x2ajdFcdUY0RkurOPJagkXXgC4hOCjQYvc6elHz1sGb_n0W3V5fLSffR7PFt-nkYjYSOU26URrzCsuyFJkgADytyhJoXWCacFnikAKSQpFRUgOHlCSCJ0RUGRWcQyalJGfR-4PvtjWO9W11DOcUx2lMsjIQ0wMhDV-zrVUbbnfMcMXuA8auGLedEi2wvKZlUci8jCuaxiC5yItcVhJTkUhKquD1ta_mqw1IATr0sB2YDjNaNWxl7liahomVRTD41BtY88uD61joq4C25RqMP_x3RvMyIQH98A_69Ol6asXDAZSuTagr9qbsIg1tJKFsFqjxE1R4JGxUuDBhbCE-EHweCALTwe9uxb1zbPrj5v_Zxc8h-_GIbYC3XeNM6-8v3RBMD6CwxjkL9WOTccz2K_TQDbZfIdavUJC9Ox7Qo-hhZ8hfBE0aRg</recordid><startdate>20150624</startdate><enddate>20150624</enddate><creator>Vigani, Gianpiero</creator><creator>Faoro, Franco</creator><creator>Ferretti, Anna Maria</creator><creator>Cantele, Francesca</creator><creator>Maffi, Dario</creator><creator>Marelli, Marcello</creator><creator>Maver, Mauro</creator><creator>Murgia, Irene</creator><creator>Zocchi, Graziano</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150624</creationdate><title>Three-Dimensional Reconstruction, by TEM Tomography, of the Ultrastructural Modifications Occurring in Cucumis sativus L. Mitochondria under Fe Deficiency</title><author>Vigani, Gianpiero ; Faoro, Franco ; Ferretti, Anna Maria ; Cantele, Francesca ; Maffi, Dario ; Marelli, Marcello ; Maver, Mauro ; Murgia, Irene ; Zocchi, Graziano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-40ab1d88c5c3eea4b88e9f7192ad81b1de34e7593feae432ca23cb59caae5ddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Apoptosis</topic><topic>Cellular structure</topic><topic>Chloroplasts</topic><topic>Conformation</topic><topic>Cristae</topic><topic>Cucumis sativus</topic><topic>Cucumis sativus - metabolism</topic><topic>Cucumis sativus - ultrastructure</topic><topic>Electron Microscope Tomography</topic><topic>Electron microscopy</topic><topic>Electron transport</topic><topic>Electron Transport - physiology</topic><topic>Homeostasis</topic><topic>Iron</topic><topic>Iron - deficiency</topic><topic>Leaves</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Mitochondria - 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Mitochondria under Fe Deficiency</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-24</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0129141</spage><epage>e0129141</epage><pages>e0129141-e0129141</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mitochondria, as recently suggested, might be involved in iron sensing and signalling pathways in plant cells. For a better understanding of the role of these organelles in mediating the Fe deficiency responses in plant cells, it is crucial to provide a full overview of their modifications occurring under Fe-limited conditions. The aim of this work is to characterize the ultrastructural as well as the biochemical changes occurring in leaf mitochondria of cucumber (Cucumis sativus L.) plants grown under Fe deficiency.
Mitochondrial ultrastructure was investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allowed a three-dimensional (3D) reconstruction of cellular structures. These analyses reveal that mitochondria isolated from cucumber leaves appear in the cristae junction model conformation and that Fe deficiency strongly alters both the number and the volume of cristae. The ultrastructural changes observed in mitochondria isolated from Fe-deficient leaves reflect a metabolic status characterized by a respiratory chain operating at a lower rate (orthodox-like conformation) with respect to mitochondria from control leaves.
To our knowledge, this is the first report showing a 3D reconstruction of plant mitochondria. Furthermore, these results suggest that a detailed characterization of the link between changes in the ultrastructure and functionality of mitochondria during different nutritional conditions, can provide a successful approach to understand the role of these organelles in the plant response to Fe deficiency.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26107946</pmid><doi>10.1371/journal.pone.0129141</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| subjects | Apoptosis Cellular structure Chloroplasts Conformation Cristae Cucumis sativus Cucumis sativus - metabolism Cucumis sativus - ultrastructure Electron Microscope Tomography Electron microscopy Electron transport Electron Transport - physiology Homeostasis Iron Iron - deficiency Leaves Metabolism Mitochondria Mitochondria - metabolism Mitochondria - ultrastructure Mitochondrial DNA Organelles Plant cells Plant Leaves - metabolism Plant Leaves - ultrastructure Plant mitochondria Reconstruction Respiration Signal Transduction Tomography Transmission electron microscopy Ultrastructure |
| title | Three-Dimensional Reconstruction, by TEM Tomography, of the Ultrastructural Modifications Occurring in Cucumis sativus L. Mitochondria under Fe Deficiency |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T06%3A10%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Three-Dimensional%20Reconstruction,%20by%20TEM%20Tomography,%20of%20the%20Ultrastructural%20Modifications%20Occurring%20in%20Cucumis%20sativus%20L.%20Mitochondria%20under%20Fe%20Deficiency&rft.jtitle=PloS%20one&rft.au=Vigani,%20Gianpiero&rft.date=2015-06-24&rft.volume=10&rft.issue=6&rft.spage=e0129141&rft.epage=e0129141&rft.pages=e0129141-e0129141&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0129141&rft_dat=%3Cgale_plos_%3EA419239485%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1691040358&rft_id=info:pmid/26107946&rft_galeid=A419239485&rft_doaj_id=oai_doaj_org_article_6f9877d680b940edac676dbd19c2d93b&rfr_iscdi=true |