Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model
Skeletal muscle injury is the most common problem in orthopedic and sports medicine, and severe injury leads to fibrosis and muscle dysfunction. Conventional treatment for successive muscle injury is currently controversial, although new therapies, like cell therapy, seem to be promise. We developed...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-06, Vol.10 (6), p.e0127561-e0127561 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0127561 |
|---|---|
| container_issue | 6 |
| container_start_page | e0127561 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Andrade, Bruno M Baldanza, Marcelo R Ribeiro, Karla C Porto, Anderson Peçanha, Ramon Fortes, Fabio S A Zapata-Sudo, Gisele Campos-de-Carvalho, Antonio C Goldenberg, Regina C S Werneck-de-Castro, João Pedro |
| description | Skeletal muscle injury is the most common problem in orthopedic and sports medicine, and severe injury leads to fibrosis and muscle dysfunction. Conventional treatment for successive muscle injury is currently controversial, although new therapies, like cell therapy, seem to be promise. We developed a model of successive injuries in rat to evaluate the therapeutic potential of bone marrow mesenchymal cells (BMMC) injected directly into the injured muscle. Functional and histological assays were performed 14 and 28 days after the injury protocol by isometric tension recording and picrosirius/Hematoxilin & Eosin staining, respectively. We also evaluated the presence and the fate of BMMC on treated muscles; and muscle fiber regeneration. BMMC treatment increased maximal skeletal muscle contraction 14 and 28 days after muscle injury compared to non-treated group (4.5 ± 1.7 vs 2.5 ± 0.98 N/cm2, p |
| doi_str_mv | 10.1371/journal.pone.0127561 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1690846335</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A426203476</galeid><doaj_id>oai_doaj_org_article_20d8769a58c34a00a06ebee26601523a</doaj_id><sourcerecordid>A426203476</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-3af7a374790097af562bdbba8e2058ba62a2ba4b91a13736161be3365c7a9ee23</originalsourceid><addsrcrecordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MVfceILUqn4WKlSJb4OXKxJMtn14sRbOynsv8dh06pBPSAfYtnPvJN3PJMkTylZUp7TN1s3-A7scuc6XBLK8kzSe8kxVZwtJCP8_q39UfIohC0hGS-kfJgcMUm4YoIfJz_exfC0Be_dr7TFgF212bdg0wqtDalpd95dRWAIlcW0GbqqN65LTZdCGn6ixT6y063Hhem2g9-nravRPk4eNGADPpm-J8m3D--_nn1anF98XJ2dni8qqVi_4NDkwHORK0JUDk0mWVmXJRTISFaUIBmwEkSpKETfXFJJS-RcZlUOCpHxk-T5QXdnXdBTWYKmUpFCSM6zSKwORO1gq3feRL977cDovwfOrzX43kQPmpG6yKWCrKi4AEKASCxjFikJzRiHqPV2yjaULdYVdr0HOxOd33Rmo9fuSguRCcGLKPBqEvDucsDQ69aEsdrQoRvG_y6koEwoFdEX_6B3u5uoNUQDpmtczFuNovpUsPH5RS4jtbyDiqvG1lSxCRoTz2cBr2cBkenxd7-GIQS9-vL5_9mL73P25S12g2D7TXB2GPsqzEFxACvvQvDY3BSZEj2OwHU19DgCehqBGPbs9gPdBF33PP8D0S0Aaw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1690846335</pqid></control><display><type>article</type><title>Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Andrade, Bruno M ; Baldanza, Marcelo R ; Ribeiro, Karla C ; Porto, Anderson ; Peçanha, Ramon ; Fortes, Fabio S A ; Zapata-Sudo, Gisele ; Campos-de-Carvalho, Antonio C ; Goldenberg, Regina C S ; Werneck-de-Castro, João Pedro</creator><contributor>Mezey, Eva</contributor><creatorcontrib>Andrade, Bruno M ; Baldanza, Marcelo R ; Ribeiro, Karla C ; Porto, Anderson ; Peçanha, Ramon ; Fortes, Fabio S A ; Zapata-Sudo, Gisele ; Campos-de-Carvalho, Antonio C ; Goldenberg, Regina C S ; Werneck-de-Castro, João Pedro ; Mezey, Eva</creatorcontrib><description>Skeletal muscle injury is the most common problem in orthopedic and sports medicine, and severe injury leads to fibrosis and muscle dysfunction. Conventional treatment for successive muscle injury is currently controversial, although new therapies, like cell therapy, seem to be promise. We developed a model of successive injuries in rat to evaluate the therapeutic potential of bone marrow mesenchymal cells (BMMC) injected directly into the injured muscle. Functional and histological assays were performed 14 and 28 days after the injury protocol by isometric tension recording and picrosirius/Hematoxilin & Eosin staining, respectively. We also evaluated the presence and the fate of BMMC on treated muscles; and muscle fiber regeneration. BMMC treatment increased maximal skeletal muscle contraction 14 and 28 days after muscle injury compared to non-treated group (4.5 ± 1.7 vs 2.5 ± 0.98 N/cm2, p<0.05 and 8.4 ± 2.3 vs. 5.7 ± 1.3 N/cm2, p<0.05 respectively). Furthermore, BMMC treatment increased muscle fiber cross-sectional area and the presence of mature muscle fiber 28 days after muscle injury. However, there was no difference in collagen deposition between groups. Immunoassays for cytoskeleton markers of skeletal and smooth muscle cells revealed an apparent integration of the BMMC within the muscle. These data suggest that BMMC transplantation accelerates and improves muscle function recovery in our extensive muscle re-injury model.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0127561</identifier><identifier>PMID: 26039243</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Bone marrow ; Bone marrow cells ; Bone Marrow Cells - metabolism ; Collagen ; Cytoskeleton ; Data recovery ; Fibrosis ; Growth factors ; Health aspects ; Immunoassays ; Injuries ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; Muscle Contraction ; Muscle function ; Muscle, Skeletal - injuries ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - physiopathology ; Muscles ; Musculoskeletal system ; Physiological aspects ; Physiology ; Rats ; Rats, Wistar ; Recovery of Function ; Regeneration ; Rodents ; Skeletal muscle ; Smooth muscle ; Sports medicine ; Stem cells ; Transplantation ; Transplants & implants</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0127561-e0127561</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Andrade et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Andrade et al 2015 Andrade et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3af7a374790097af562bdbba8e2058ba62a2ba4b91a13736161be3365c7a9ee23</citedby><cites>FETCH-LOGICAL-c692t-3af7a374790097af562bdbba8e2058ba62a2ba4b91a13736161be3365c7a9ee23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454438/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454438/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26039243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mezey, Eva</contributor><creatorcontrib>Andrade, Bruno M</creatorcontrib><creatorcontrib>Baldanza, Marcelo R</creatorcontrib><creatorcontrib>Ribeiro, Karla C</creatorcontrib><creatorcontrib>Porto, Anderson</creatorcontrib><creatorcontrib>Peçanha, Ramon</creatorcontrib><creatorcontrib>Fortes, Fabio S A</creatorcontrib><creatorcontrib>Zapata-Sudo, Gisele</creatorcontrib><creatorcontrib>Campos-de-Carvalho, Antonio C</creatorcontrib><creatorcontrib>Goldenberg, Regina C S</creatorcontrib><creatorcontrib>Werneck-de-Castro, João Pedro</creatorcontrib><title>Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Skeletal muscle injury is the most common problem in orthopedic and sports medicine, and severe injury leads to fibrosis and muscle dysfunction. Conventional treatment for successive muscle injury is currently controversial, although new therapies, like cell therapy, seem to be promise. We developed a model of successive injuries in rat to evaluate the therapeutic potential of bone marrow mesenchymal cells (BMMC) injected directly into the injured muscle. Functional and histological assays were performed 14 and 28 days after the injury protocol by isometric tension recording and picrosirius/Hematoxilin & Eosin staining, respectively. We also evaluated the presence and the fate of BMMC on treated muscles; and muscle fiber regeneration. BMMC treatment increased maximal skeletal muscle contraction 14 and 28 days after muscle injury compared to non-treated group (4.5 ± 1.7 vs 2.5 ± 0.98 N/cm2, p<0.05 and 8.4 ± 2.3 vs. 5.7 ± 1.3 N/cm2, p<0.05 respectively). Furthermore, BMMC treatment increased muscle fiber cross-sectional area and the presence of mature muscle fiber 28 days after muscle injury. However, there was no difference in collagen deposition between groups. Immunoassays for cytoskeleton markers of skeletal and smooth muscle cells revealed an apparent integration of the BMMC within the muscle. These data suggest that BMMC transplantation accelerates and improves muscle function recovery in our extensive muscle re-injury model.</description><subject>Animals</subject><subject>Bone marrow</subject><subject>Bone marrow cells</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Collagen</subject><subject>Cytoskeleton</subject><subject>Data recovery</subject><subject>Fibrosis</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Immunoassays</subject><subject>Injuries</subject><subject>Male</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Muscle Contraction</subject><subject>Muscle function</subject><subject>Muscle, Skeletal - injuries</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recovery of Function</subject><subject>Regeneration</subject><subject>Rodents</subject><subject>Skeletal muscle</subject><subject>Smooth muscle</subject><subject>Sports medicine</subject><subject>Stem cells</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkJw2MVfceILUqn4WKlSJb4OXKxJMtn14sRbOynsv8dh06pBPSAfYtnPvJN3PJMkTylZUp7TN1s3-A7scuc6XBLK8kzSe8kxVZwtJCP8_q39UfIohC0hGS-kfJgcMUm4YoIfJz_exfC0Be_dr7TFgF212bdg0wqtDalpd95dRWAIlcW0GbqqN65LTZdCGn6ixT6y063Hhem2g9-nravRPk4eNGADPpm-J8m3D--_nn1anF98XJ2dni8qqVi_4NDkwHORK0JUDk0mWVmXJRTISFaUIBmwEkSpKETfXFJJS-RcZlUOCpHxk-T5QXdnXdBTWYKmUpFCSM6zSKwORO1gq3feRL977cDovwfOrzX43kQPmpG6yKWCrKi4AEKASCxjFikJzRiHqPV2yjaULdYVdr0HOxOd33Rmo9fuSguRCcGLKPBqEvDucsDQ69aEsdrQoRvG_y6koEwoFdEX_6B3u5uoNUQDpmtczFuNovpUsPH5RS4jtbyDiqvG1lSxCRoTz2cBr2cBkenxd7-GIQS9-vL5_9mL73P25S12g2D7TXB2GPsqzEFxACvvQvDY3BSZEj2OwHU19DgCehqBGPbs9gPdBF33PP8D0S0Aaw</recordid><startdate>20150603</startdate><enddate>20150603</enddate><creator>Andrade, Bruno M</creator><creator>Baldanza, Marcelo R</creator><creator>Ribeiro, Karla C</creator><creator>Porto, Anderson</creator><creator>Peçanha, Ramon</creator><creator>Fortes, Fabio S A</creator><creator>Zapata-Sudo, Gisele</creator><creator>Campos-de-Carvalho, Antonio C</creator><creator>Goldenberg, Regina C S</creator><creator>Werneck-de-Castro, João Pedro</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150603</creationdate><title>Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model</title><author>Andrade, Bruno M ; Baldanza, Marcelo R ; Ribeiro, Karla C ; Porto, Anderson ; Peçanha, Ramon ; Fortes, Fabio S A ; Zapata-Sudo, Gisele ; Campos-de-Carvalho, Antonio C ; Goldenberg, Regina C S ; Werneck-de-Castro, João Pedro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3af7a374790097af562bdbba8e2058ba62a2ba4b91a13736161be3365c7a9ee23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bone marrow</topic><topic>Bone marrow cells</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Collagen</topic><topic>Cytoskeleton</topic><topic>Data recovery</topic><topic>Fibrosis</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Immunoassays</topic><topic>Injuries</topic><topic>Male</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchyme</topic><topic>Muscle Contraction</topic><topic>Muscle function</topic><topic>Muscle, Skeletal - injuries</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recovery of Function</topic><topic>Regeneration</topic><topic>Rodents</topic><topic>Skeletal muscle</topic><topic>Smooth muscle</topic><topic>Sports medicine</topic><topic>Stem cells</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrade, Bruno M</creatorcontrib><creatorcontrib>Baldanza, Marcelo R</creatorcontrib><creatorcontrib>Ribeiro, Karla C</creatorcontrib><creatorcontrib>Porto, Anderson</creatorcontrib><creatorcontrib>Peçanha, Ramon</creatorcontrib><creatorcontrib>Fortes, Fabio S A</creatorcontrib><creatorcontrib>Zapata-Sudo, Gisele</creatorcontrib><creatorcontrib>Campos-de-Carvalho, Antonio C</creatorcontrib><creatorcontrib>Goldenberg, Regina C S</creatorcontrib><creatorcontrib>Werneck-de-Castro, João Pedro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrade, Bruno M</au><au>Baldanza, Marcelo R</au><au>Ribeiro, Karla C</au><au>Porto, Anderson</au><au>Peçanha, Ramon</au><au>Fortes, Fabio S A</au><au>Zapata-Sudo, Gisele</au><au>Campos-de-Carvalho, Antonio C</au><au>Goldenberg, Regina C S</au><au>Werneck-de-Castro, João Pedro</au><au>Mezey, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-03</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0127561</spage><epage>e0127561</epage><pages>e0127561-e0127561</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Skeletal muscle injury is the most common problem in orthopedic and sports medicine, and severe injury leads to fibrosis and muscle dysfunction. Conventional treatment for successive muscle injury is currently controversial, although new therapies, like cell therapy, seem to be promise. We developed a model of successive injuries in rat to evaluate the therapeutic potential of bone marrow mesenchymal cells (BMMC) injected directly into the injured muscle. Functional and histological assays were performed 14 and 28 days after the injury protocol by isometric tension recording and picrosirius/Hematoxilin & Eosin staining, respectively. We also evaluated the presence and the fate of BMMC on treated muscles; and muscle fiber regeneration. BMMC treatment increased maximal skeletal muscle contraction 14 and 28 days after muscle injury compared to non-treated group (4.5 ± 1.7 vs 2.5 ± 0.98 N/cm2, p<0.05 and 8.4 ± 2.3 vs. 5.7 ± 1.3 N/cm2, p<0.05 respectively). Furthermore, BMMC treatment increased muscle fiber cross-sectional area and the presence of mature muscle fiber 28 days after muscle injury. However, there was no difference in collagen deposition between groups. Immunoassays for cytoskeleton markers of skeletal and smooth muscle cells revealed an apparent integration of the BMMC within the muscle. These data suggest that BMMC transplantation accelerates and improves muscle function recovery in our extensive muscle re-injury model.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26039243</pmid><doi>10.1371/journal.pone.0127561</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-06, Vol.10 (6), p.e0127561-e0127561 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1690846335 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Bone marrow Bone marrow cells Bone Marrow Cells - metabolism Collagen Cytoskeleton Data recovery Fibrosis Growth factors Health aspects Immunoassays Injuries Male Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - metabolism Mesenchyme Muscle Contraction Muscle function Muscle, Skeletal - injuries Muscle, Skeletal - metabolism Muscle, Skeletal - physiopathology Muscles Musculoskeletal system Physiological aspects Physiology Rats Rats, Wistar Recovery of Function Regeneration Rodents Skeletal muscle Smooth muscle Sports medicine Stem cells Transplantation Transplants & implants |
| title | Bone marrow mesenchymal cells improve muscle function in a skeletal muscle re-injury model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T14%3A42%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bone%20marrow%20mesenchymal%20cells%20improve%20muscle%20function%20in%20a%20skeletal%20muscle%20re-injury%20model&rft.jtitle=PloS%20one&rft.au=Andrade,%20Bruno%20M&rft.date=2015-06-03&rft.volume=10&rft.issue=6&rft.spage=e0127561&rft.epage=e0127561&rft.pages=e0127561-e0127561&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0127561&rft_dat=%3Cgale_plos_%3EA426203476%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1690846335&rft_id=info:pmid/26039243&rft_galeid=A426203476&rft_doaj_id=oai_doaj_org_article_20d8769a58c34a00a06ebee26601523a&rfr_iscdi=true |