Conditional U1 Gene Silencing in Toxoplasma gondii

The functional characterisation of essential genes in apicomplexan parasites, such as Toxoplasma gondii or Plasmodium falciparum, relies on conditional mutagenesis systems. Here we present a novel strategy based on U1 snRNP-mediated gene silencing. U1 snRNP is critical in pre-mRNA splicing by defini...

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Veröffentlicht in:	PloS one 2015-06, Vol.10 (6), p.e0130356
Hauptverfasser:	Pieperhoff, Manuela S, Pall, Gurman S, Jiménez-Ruiz, Elena, Das, Sujaan, Melatti, Carmen, Gow, Matthew, Wong, Eleanor H, Heng, Joanne, Müller, Sylke, Blackman, Michael J, Meissner, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Base Sequence Binding Sites C gene Clathrin Clathrin Heavy Chains - genetics Cloning Dihydrofolate reductase Dynamin Exons Gene Expression Gene Silencing Gene Targeting Genes Genes, Reporter Genetic engineering Genetic Loci Genetic Vectors - genetics Genomes Homologous Recombination Infections Inflammation Kinases Life sciences Malaria Medical research Molecular Sequence Data mRNA Mutagenesis Mutants Nucleic Acid Conformation Nucleotide Motifs Parasites Parasitology Pathogens Plasmodium Plasmodium falciparum Plasmodium falciparum - genetics Protein Binding Protein C Protein transport Proteins Protozoa Rapamycin Recognition Reporter gene Ribonucleoprotein, U1 Small Nuclear - chemistry Ribonucleoprotein, U1 Small Nuclear - genetics Ribonucleoprotein, U1 Small Nuclear - metabolism Ribonucleoproteins (small nuclear) RNA RNA Precursors - chemistry RNA Precursors - genetics RNA Precursors - metabolism Rodents Sequence Alignment Splicing Toxoplasma - genetics Toxoplasma gondii
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