Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had...
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| creator | Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki |
| description | A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions. |
| doi_str_mv | 10.1371/journal.pone.0130208 |
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To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0130208</identifier><identifier>PMID: 26079133</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adaptation, Physiological ; Amino Acid Substitution ; Amino acids ; Analysis ; Animals ; Antigens ; Antisera ; Chickens ; Dogs ; Eggs ; Female ; Ferrets - virology ; Growth rate ; Hemagglutinins ; Host-Pathogen Interactions ; Influenza ; Influenza A ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza A Virus, H1N1 Subtype - physiology ; Japan ; Kinetics ; Madin Darby Canine Kidney Cells ; Mice ; Ovum - virology ; Pandemics ; Pathogenicity ; Pathogens ; Poultry ; Swine flu ; Vaccines ; Viruses</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0130208-e0130208</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ainai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Ainai et al 2015 Ainai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-1e8e3078aecca0c6002ff30522305cb2b3818bd515b17719ecdcad3e816c61ce3</citedby><cites>FETCH-LOGICAL-c758t-1e8e3078aecca0c6002ff30522305cb2b3818bd515b17719ecdcad3e816c61ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26079133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Schmolke, Mirco</contributor><creatorcontrib>Ainai, Akira</creatorcontrib><creatorcontrib>Hasegawa, Hideki</creatorcontrib><creatorcontrib>Obuchi, Masatsugu</creatorcontrib><creatorcontrib>Odagiri, Takato</creatorcontrib><creatorcontrib>Ujike, Makoto</creatorcontrib><creatorcontrib>Shirakura, Masayuki</creatorcontrib><creatorcontrib>Nobusawa, Eri</creatorcontrib><creatorcontrib>Tashiro, Masato</creatorcontrib><creatorcontrib>Asanuma, Hideki</creatorcontrib><title>Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions.</description><subject>Acids</subject><subject>Adaptation, Physiological</subject><subject>Amino Acid Substitution</subject><subject>Amino acids</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antisera</subject><subject>Chickens</subject><subject>Dogs</subject><subject>Eggs</subject><subject>Female</subject><subject>Ferrets - virology</subject><subject>Growth rate</subject><subject>Hemagglutinins</subject><subject>Host-Pathogen Interactions</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H1N1 Subtype - immunology</subject><subject>Influenza A Virus, H1N1 Subtype - physiology</subject><subject>Japan</subject><subject>Kinetics</subject><subject>Madin Darby Canine Kidney Cells</subject><subject>Mice</subject><subject>Ovum - virology</subject><subject>Pandemics</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Poultry</subject><subject>Swine flu</subject><subject>Vaccines</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1tv0zAUxyMEYmPwDRBEQkLw0M6XxHFekKqJ0aKJIS57tVz7pPWU2pntIODT49BsatAeUKQkOv79z83nZNlzjOaYVvj02vXeynbeOQtzhCkiiD_IjnFNyYwRRB8e_B9lT0K4RqiknLHH2RFhqKoxpcfZzdKFmC-07KKMxtlcWp3HLeSLNoLfm1yTXxkv2_yzdx34aCAMtoE6Nz7JV7Zpe7C_Zb44XeJPuNM7VA-aPuSr4FoZQefG5h9lJ-3T7FEj2wDPxu9J9v38_bez5ezi8sPqbHExU1XJ4wwDB4oqLkEpiRRDiDQNRSUh6aXWZE055mtd4nKNqwrXoLSSmgLHTDGsgJ5kL_d-u9YFMXYrCMx4TVGqHyditSe0k9ei82Yn_S_hpBF_Dc5vhEzVqhYEI6qpSV0gXteFVHWKpIcOqoqXlW6K5OvdGK1f70ArsDF1bOJ0emLNVmzcD1EULOUzJPNmdODdTQ8hip0JCtpWWnD9Pm9eEFrzhL76B72_upHayFSAsY1LcdXgVCwKzHHFWEUSNb-HSo-GnVFptBqT7BPB24kgMRF-xo3sQxCrr1_-n728mrKvD9gtyDZu0-j0wwSGKVjsQeVdCB6auyZjJIbNuO2GGDZDjJuRZC8OL-hOdLsK9A9pxQcy</recordid><startdate>20150616</startdate><enddate>20150616</enddate><creator>Ainai, Akira</creator><creator>Hasegawa, Hideki</creator><creator>Obuchi, Masatsugu</creator><creator>Odagiri, Takato</creator><creator>Ujike, Makoto</creator><creator>Shirakura, Masayuki</creator><creator>Nobusawa, Eri</creator><creator>Tashiro, Masato</creator><creator>Asanuma, Hideki</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150616</creationdate><title>Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan</title><author>Ainai, Akira ; Hasegawa, Hideki ; Obuchi, Masatsugu ; Odagiri, Takato ; Ujike, Makoto ; Shirakura, Masayuki ; Nobusawa, Eri ; Tashiro, Masato ; Asanuma, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-1e8e3078aecca0c6002ff30522305cb2b3818bd515b17719ecdcad3e816c61ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acids</topic><topic>Adaptation, Physiological</topic><topic>Amino Acid Substitution</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antigens</topic><topic>Antisera</topic><topic>Chickens</topic><topic>Dogs</topic><topic>Eggs</topic><topic>Female</topic><topic>Ferrets - virology</topic><topic>Growth rate</topic><topic>Hemagglutinins</topic><topic>Host-Pathogen Interactions</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza A Virus, H1N1 Subtype - immunology</topic><topic>Influenza A Virus, H1N1 Subtype - physiology</topic><topic>Japan</topic><topic>Kinetics</topic><topic>Madin Darby Canine Kidney Cells</topic><topic>Mice</topic><topic>Ovum - virology</topic><topic>Pandemics</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Poultry</topic><topic>Swine flu</topic><topic>Vaccines</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ainai, Akira</creatorcontrib><creatorcontrib>Hasegawa, Hideki</creatorcontrib><creatorcontrib>Obuchi, Masatsugu</creatorcontrib><creatorcontrib>Odagiri, Takato</creatorcontrib><creatorcontrib>Ujike, Makoto</creatorcontrib><creatorcontrib>Shirakura, Masayuki</creatorcontrib><creatorcontrib>Nobusawa, Eri</creatorcontrib><creatorcontrib>Tashiro, Masato</creatorcontrib><creatorcontrib>Asanuma, Hideki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ainai, Akira</au><au>Hasegawa, Hideki</au><au>Obuchi, Masatsugu</au><au>Odagiri, Takato</au><au>Ujike, Makoto</au><au>Shirakura, Masayuki</au><au>Nobusawa, Eri</au><au>Tashiro, Masato</au><au>Asanuma, Hideki</au><au>Schmolke, Mirco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-16</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0130208</spage><epage>e0130208</epage><pages>e0130208-e0130208</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26079133</pmid><doi>10.1371/journal.pone.0130208</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Adaptation, Physiological Amino Acid Substitution Amino acids Analysis Animals Antigens Antisera Chickens Dogs Eggs Female Ferrets - virology Growth rate Hemagglutinins Host-Pathogen Interactions Influenza Influenza A Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H1N1 Subtype - physiology Japan Kinetics Madin Darby Canine Kidney Cells Mice Ovum - virology Pandemics Pathogenicity Pathogens Poultry Swine flu Vaccines Viruses |
| title | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
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