RNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

The transformer (tra) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 (tra-2) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster. In this study, we describe the isolation, expression and function...

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Veröffentlicht in:PloS one 2015-06, Vol.10 (6), p.e0128892-e0128892
Hauptverfasser: Liu, Guiqing, Wu, Qiang, Li, Jianwei, Zhang, Guifen, Wan, Fanghao
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Wu, Qiang
Li, Jianwei
Zhang, Guifen
Wan, Fanghao
description The transformer (tra) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 (tra-2) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster. In this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, Bactrocera dorsalis (Hendel). Bdtra and Bdtra-2 are similar to their homologs from other tephritid species. Bdtra demonstrated sex-specific transcripts: one transcript in females and two transcripts in males. In contrast, Bdtra-2 only had one transcript that was common to males and females, which was transcribed continuously in different adult tissues and developmental stages. Bdtra-2 and the female form of Bdtra were maternally inherited in eggs, whereas the male form of Bdtra was not detectable until embryos of 1 and 2 h after egg laying. Function analyses of Bdtra and Bdtra-2 indicated that both were indispensable for female development, as nearly 100% males were obtained with embryonic RNAi against either Bdtra or Bdtra-2. The fertility of these RNAi-generated males was subsequently tested. More than 80% of RNAi-generated males could mate and the mated females could lay eggs, but only 40-48.6% males gave rise to progeny. In XX-reversed males and intersex individuals, no clear female gonadal morphology was observed after dissection. These results shed light on the development of a genetic sexing system with male-only release for this agricultural pest.
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In this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, Bactrocera dorsalis (Hendel). Bdtra and Bdtra-2 are similar to their homologs from other tephritid species. Bdtra demonstrated sex-specific transcripts: one transcript in females and two transcripts in males. In contrast, Bdtra-2 only had one transcript that was common to males and females, which was transcribed continuously in different adult tissues and developmental stages. Bdtra-2 and the female form of Bdtra were maternally inherited in eggs, whereas the male form of Bdtra was not detectable until embryos of 1 and 2 h after egg laying. Function analyses of Bdtra and Bdtra-2 indicated that both were indispensable for female development, as nearly 100% males were obtained with embryonic RNAi against either Bdtra or Bdtra-2. The fertility of these RNAi-generated males was subsequently tested. More than 80% of RNAi-generated males could mate and the mated females could lay eggs, but only 40-48.6% males gave rise to progeny. In XX-reversed males and intersex individuals, no clear female gonadal morphology was observed after dissection. These results shed light on the development of a genetic sexing system with male-only release for this agricultural pest.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26057559</pmid><doi>10.1371/journal.pone.0128892</doi><oa>free_for_read</oa></addata></record>
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subjects Agricultural pests
Agriculture
Agronomy
Animals
Bactrocera dorsalis
Biology
Ceratitis capitata
Developmental stages
Diptera
Diptera - genetics
Diptera - physiology
Drosophila
Egg laying
Eggs
Embryogenesis
Embryos
Female
Females
Fertility
Fruit flies
Fruits
Gene expression
Gene Silencing
Genetic engineering
Homology
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
Laboratories
Male
Males
Mediterranean fruit fly
Offspring
Pests
Plant diseases
Progeny
Proteins
RNA interference
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
RNA-mediated interference
Sex Determination Processes - genetics
Sex Differentiation - genetics
Sexing
Tephritidae
Transcription
Transformer (tra) gene
title RNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)
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