Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening
A comprehensive investigation of abscisic acid (ABA) biosynthesis and its influence on other important phytochemicals is critical for understanding the versatile roles that ABA plays during strawberry fruit ripening. Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihy...
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| description | A comprehensive investigation of abscisic acid (ABA) biosynthesis and its influence on other important phytochemicals is critical for understanding the versatile roles that ABA plays during strawberry fruit ripening. Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihydroguaiaretic acid (NDGA; an ABA biosynthesis blocker) treatment during ripening and assessed the expression changes of genes involved in the metabolism of pigments, ascorbic acid (AsA) and folic acid in the receptacles. The transcriptome analysis identified a lot of genes differentially expressed in response to ABA or NDGA treatment. In particular, genes in the anthocyanin biosynthesis pathway were actively regulated by ABA, with the exception of the gene encoding cinnamate 4-hydroxylase. Chlorophyll degradation was accelerated by ABA mainly owing to the higher expression of gene encoding pheide a oxygenase. The decrease of β-carotene content was accelerated by ABA treatment and delayed by NDGA. A high negative correlation rate was found between ABA and β-carotene content, indicating the importance of the requirement for ABA synthesis during fruit ripening. In addition, evaluation on the folate biosynthetic pathway indicate that ABA might have minor function in this nutrient's biosynthesis process, however, it might be involved in its homeostasis. Surprisingly, though AsA content accumulated during fruit ripening, expressions of genes involved in its biosynthesis in the receptacles were significantly lower in ABA-treated fruits. This transcriptome analysis expands our understanding of ABA's role in phytochemical metabolism during strawberry fruit ripening and the regulatory mechanisms of ABA on these pathways were discussed. Our study provides a wealth of genetic information in the metabolism pathways and may be helpful for molecular manipulation in the future. |
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Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihydroguaiaretic acid (NDGA; an ABA biosynthesis blocker) treatment during ripening and assessed the expression changes of genes involved in the metabolism of pigments, ascorbic acid (AsA) and folic acid in the receptacles. The transcriptome analysis identified a lot of genes differentially expressed in response to ABA or NDGA treatment. In particular, genes in the anthocyanin biosynthesis pathway were actively regulated by ABA, with the exception of the gene encoding cinnamate 4-hydroxylase. Chlorophyll degradation was accelerated by ABA mainly owing to the higher expression of gene encoding pheide a oxygenase. The decrease of β-carotene content was accelerated by ABA treatment and delayed by NDGA. A high negative correlation rate was found between ABA and β-carotene content, indicating the importance of the requirement for ABA synthesis during fruit ripening. In addition, evaluation on the folate biosynthetic pathway indicate that ABA might have minor function in this nutrient's biosynthesis process, however, it might be involved in its homeostasis. Surprisingly, though AsA content accumulated during fruit ripening, expressions of genes involved in its biosynthesis in the receptacles were significantly lower in ABA-treated fruits. This transcriptome analysis expands our understanding of ABA's role in phytochemical metabolism during strawberry fruit ripening and the regulatory mechanisms of ABA on these pathways were discussed. Our study provides a wealth of genetic information in the metabolism pathways and may be helpful for molecular manipulation in the future.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0130037</identifier><identifier>PMID: 26053069</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abscisic acid ; Abscisic Acid - metabolism ; Acids ; Analysis ; Ascorbic acid ; Ascorbic Acid - metabolism ; Beta carotene ; Biosynthesis ; Carotenoids ; Chlorophyll ; Cinnamate 4-hydroxylase ; Citrus fruits ; Dehydrogenases ; Engineering ; Environmental degradation ; Enzymes ; Folic acid ; Folic Acid - metabolism ; Food science ; Fragaria ; Fragaria - genetics ; Fragaria - growth & development ; Fragaria - metabolism ; Fruit - growth & development ; Fruit - metabolism ; Fruits ; Fruits (Food) ; Gene expression ; Genes ; Homeostasis ; Hydroxylase ; Hydroxylases ; Kinases ; Laboratories ; Metabolism ; Metabolites ; Molecular chains ; Nordihydroguaiaretic acid ; Oxygenase ; Phytochemicals ; Pigments ; Pigments, Biological - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Regulatory mechanisms (biology) ; Ribonucleic acid ; Ripening ; RNA ; Transcriptome ; Vitamin B ; Vitamin C ; Vitamin deficiency ; β-Carotene</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0130037-e0130037</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Li et al 2015 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-fba2cdd1fe5bca72128d13cd48f366fba6ac6bcff9b0c4f53dea36be9e3fe4ad3</citedby><cites>FETCH-LOGICAL-c692t-fba2cdd1fe5bca72128d13cd48f366fba6ac6bcff9b0c4f53dea36be9e3fe4ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460069/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460069/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26053069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lu, Wang-jin</contributor><creatorcontrib>Li, Dongdong</creatorcontrib><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Luo, Zisheng</creatorcontrib><creatorcontrib>Mou, Wangshu</creatorcontrib><creatorcontrib>Mao, Linchun</creatorcontrib><creatorcontrib>Ying, Tiejin</creatorcontrib><title>Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A comprehensive investigation of abscisic acid (ABA) biosynthesis and its influence on other important phytochemicals is critical for understanding the versatile roles that ABA plays during strawberry fruit ripening. Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihydroguaiaretic acid (NDGA; an ABA biosynthesis blocker) treatment during ripening and assessed the expression changes of genes involved in the metabolism of pigments, ascorbic acid (AsA) and folic acid in the receptacles. The transcriptome analysis identified a lot of genes differentially expressed in response to ABA or NDGA treatment. In particular, genes in the anthocyanin biosynthesis pathway were actively regulated by ABA, with the exception of the gene encoding cinnamate 4-hydroxylase. Chlorophyll degradation was accelerated by ABA mainly owing to the higher expression of gene encoding pheide a oxygenase. The decrease of β-carotene content was accelerated by ABA treatment and delayed by NDGA. A high negative correlation rate was found between ABA and β-carotene content, indicating the importance of the requirement for ABA synthesis during fruit ripening. In addition, evaluation on the folate biosynthetic pathway indicate that ABA might have minor function in this nutrient's biosynthesis process, however, it might be involved in its homeostasis. Surprisingly, though AsA content accumulated during fruit ripening, expressions of genes involved in its biosynthesis in the receptacles were significantly lower in ABA-treated fruits. This transcriptome analysis expands our understanding of ABA's role in phytochemical metabolism during strawberry fruit ripening and the regulatory mechanisms of ABA on these pathways were discussed. Our study provides a wealth of genetic information in the metabolism pathways and may be helpful for molecular manipulation in the future.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Acids</subject><subject>Analysis</subject><subject>Ascorbic acid</subject><subject>Ascorbic Acid - metabolism</subject><subject>Beta carotene</subject><subject>Biosynthesis</subject><subject>Carotenoids</subject><subject>Chlorophyll</subject><subject>Cinnamate 4-hydroxylase</subject><subject>Citrus fruits</subject><subject>Dehydrogenases</subject><subject>Engineering</subject><subject>Environmental degradation</subject><subject>Enzymes</subject><subject>Folic acid</subject><subject>Folic Acid - metabolism</subject><subject>Food science</subject><subject>Fragaria</subject><subject>Fragaria - genetics</subject><subject>Fragaria - growth & development</subject><subject>Fragaria - metabolism</subject><subject>Fruit - growth & development</subject><subject>Fruit - metabolism</subject><subject>Fruits</subject><subject>Fruits (Food)</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Homeostasis</subject><subject>Hydroxylase</subject><subject>Hydroxylases</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Molecular chains</subject><subject>Nordihydroguaiaretic acid</subject><subject>Oxygenase</subject><subject>Phytochemicals</subject><subject>Pigments</subject><subject>Pigments, Biological - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Regulatory mechanisms (biology)</subject><subject>Ribonucleic acid</subject><subject>Ripening</subject><subject>RNA</subject><subject>Transcriptome</subject><subject>Vitamin B</subject><subject>Vitamin C</subject><subject>Vitamin deficiency</subject><subject>β-Carotene</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99rFDEQxxdRbK3-B6IBQRS8M9nsJrcvwlE8PahU2upryCaTu5Td5Jpkq_2H_DvNtdvSkz5IHvJjPvOdySRTFC8JnhLKycdzPwQnu-nGO5hiQjGm_FGxTxpaTliJ6eN7673iWYznGNd0xtjTYq9keYlZs1_8OfT9RgaZ7CWgsyBdVMFuku8BzbP6VbQRncAlyC6itAa0dKYbwClA3qB5G5WNVqG5shp5d018gyRb39nYb5HvdtWDS_EDmkflQ3sLS6fRIlPjVg_BuhU6TUH-aiGEK7QIg03oxG7AZcvz4onJKcCLcT4ofiw-nx1-nRwdf1kezo8mijVlmphWlkprYqBuleQlKWeaUKWrmaGMZSuTirXKmKbFqjI11SApa6EBaqCSmh4Ur290N52PYixxFITNGJ-RmpSZWN4Q2stzsQm2l-FKeGnF9YEPKyFDsqoDgXkDpeQNLxmvtKpkjaWWnOJS4aZsWdb6NEYb2h60yoUKstsR3bU4uxYrfymqiuH8fFng3SgQ_MUAMYneRgVdJx344TpvThnhNcnom3_Qh283UiuZL2Cd8Tmu2oqKeUU4qXhdzTI1fYDKQ0NvVf6PxubzHYf3Ow6ZSfA7reQQo1ienvw_e_xzl317j13nX5rW0XdDst7FXbC6AVXwMQYwd0UmWGzb6bYaYttOYmyn7Pbq_gPdOd32D_0LYDMd5Q</recordid><startdate>20150608</startdate><enddate>20150608</enddate><creator>Li, Dongdong</creator><creator>Li, Li</creator><creator>Luo, Zisheng</creator><creator>Mou, Wangshu</creator><creator>Mao, Linchun</creator><creator>Ying, Tiejin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150608</creationdate><title>Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening</title><author>Li, Dongdong ; Li, Li ; Luo, Zisheng ; Mou, Wangshu ; Mao, Linchun ; Ying, Tiejin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-fba2cdd1fe5bca72128d13cd48f366fba6ac6bcff9b0c4f53dea36be9e3fe4ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Acids</topic><topic>Analysis</topic><topic>Ascorbic acid</topic><topic>Ascorbic Acid - metabolism</topic><topic>Beta carotene</topic><topic>Biosynthesis</topic><topic>Carotenoids</topic><topic>Chlorophyll</topic><topic>Cinnamate 4-hydroxylase</topic><topic>Citrus fruits</topic><topic>Dehydrogenases</topic><topic>Engineering</topic><topic>Environmental degradation</topic><topic>Enzymes</topic><topic>Folic acid</topic><topic>Folic Acid - metabolism</topic><topic>Food science</topic><topic>Fragaria</topic><topic>Fragaria - genetics</topic><topic>Fragaria - growth & development</topic><topic>Fragaria - metabolism</topic><topic>Fruit - growth & development</topic><topic>Fruit - metabolism</topic><topic>Fruits</topic><topic>Fruits (Food)</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Homeostasis</topic><topic>Hydroxylase</topic><topic>Hydroxylases</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Molecular chains</topic><topic>Nordihydroguaiaretic acid</topic><topic>Oxygenase</topic><topic>Phytochemicals</topic><topic>Pigments</topic><topic>Pigments, Biological - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Dongdong</au><au>Li, Li</au><au>Luo, Zisheng</au><au>Mou, Wangshu</au><au>Mao, Linchun</au><au>Ying, Tiejin</au><au>Lu, Wang-jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-08</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0130037</spage><epage>e0130037</epage><pages>e0130037-e0130037</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A comprehensive investigation of abscisic acid (ABA) biosynthesis and its influence on other important phytochemicals is critical for understanding the versatile roles that ABA plays during strawberry fruit ripening. Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihydroguaiaretic acid (NDGA; an ABA biosynthesis blocker) treatment during ripening and assessed the expression changes of genes involved in the metabolism of pigments, ascorbic acid (AsA) and folic acid in the receptacles. The transcriptome analysis identified a lot of genes differentially expressed in response to ABA or NDGA treatment. In particular, genes in the anthocyanin biosynthesis pathway were actively regulated by ABA, with the exception of the gene encoding cinnamate 4-hydroxylase. Chlorophyll degradation was accelerated by ABA mainly owing to the higher expression of gene encoding pheide a oxygenase. The decrease of β-carotene content was accelerated by ABA treatment and delayed by NDGA. A high negative correlation rate was found between ABA and β-carotene content, indicating the importance of the requirement for ABA synthesis during fruit ripening. In addition, evaluation on the folate biosynthetic pathway indicate that ABA might have minor function in this nutrient's biosynthesis process, however, it might be involved in its homeostasis. Surprisingly, though AsA content accumulated during fruit ripening, expressions of genes involved in its biosynthesis in the receptacles were significantly lower in ABA-treated fruits. This transcriptome analysis expands our understanding of ABA's role in phytochemical metabolism during strawberry fruit ripening and the regulatory mechanisms of ABA on these pathways were discussed. Our study provides a wealth of genetic information in the metabolism pathways and may be helpful for molecular manipulation in the future.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26053069</pmid><doi>10.1371/journal.pone.0130037</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2015-06, Vol.10 (6), p.e0130037-e0130037 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abscisic acid Abscisic Acid - metabolism Acids Analysis Ascorbic acid Ascorbic Acid - metabolism Beta carotene Biosynthesis Carotenoids Chlorophyll Cinnamate 4-hydroxylase Citrus fruits Dehydrogenases Engineering Environmental degradation Enzymes Folic acid Folic Acid - metabolism Food science Fragaria Fragaria - genetics Fragaria - growth & development Fragaria - metabolism Fruit - growth & development Fruit - metabolism Fruits Fruits (Food) Gene expression Genes Homeostasis Hydroxylase Hydroxylases Kinases Laboratories Metabolism Metabolites Molecular chains Nordihydroguaiaretic acid Oxygenase Phytochemicals Pigments Pigments, Biological - metabolism Plant Proteins - genetics Plant Proteins - metabolism Regulatory mechanisms (biology) Ribonucleic acid Ripening RNA Transcriptome Vitamin B Vitamin C Vitamin deficiency β-Carotene |
| title | Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T12%3A09%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20Transcriptome%20Analysis%20Reveals%20the%20Influence%20of%20Abscisic%20Acid%20on%20the%20Metabolism%20of%20Pigments,%20Ascorbic%20Acid%20and%20Folic%20Acid%20during%20Strawberry%20Fruit%20Ripening&rft.jtitle=PloS%20one&rft.au=Li,%20Dongdong&rft.date=2015-06-08&rft.volume=10&rft.issue=6&rft.spage=e0130037&rft.epage=e0130037&rft.pages=e0130037-e0130037&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0130037&rft_dat=%3Cgale_plos_%3EA417147548%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1686781512&rft_id=info:pmid/26053069&rft_galeid=A417147548&rft_doaj_id=oai_doaj_org_article_079e2a7972674dc4a50ada7302c092b6&rfr_iscdi=true |