Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration
Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the...
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| Veröffentlicht in: | PloS one 2015-06, Vol.10 (6), p.e0129096-e0129096 |
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| creator | Ogata, Yusuke Mabuchi, Yo Yoshida, Mayu Suto, Eriko Grace Suzuki, Nobuharu Muneta, Takeshi Sekiya, Ichiro Akazawa, Chihiro |
| description | Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR(+) THY-1 (+) MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR (+) THY-1 (+) MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application. |
| doi_str_mv | 10.1371/journal.pone.0129096 |
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However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR(+) THY-1 (+) MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR (+) THY-1 (+) MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0129096</identifier><identifier>PMID: 26053045</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes ; Adipocytes - cytology ; Adipocytes - metabolism ; Adipogenesis ; Arthritis ; Biochemistry ; Biophysics ; Bone marrow ; Bone Marrow Cells - cytology ; Cartilage ; Cartilage - physiology ; Cell culture ; Cell Differentiation ; Cell Proliferation ; Cell Separation ; Cell surface ; Clone Cells ; Colony-Forming Units Assay ; Cytometry ; Data processing ; Differentiation ; Experiments ; Fibroblasts ; Flow cytometry ; Growth factors ; Humans ; Immunoglobulins ; Joint surgery ; Melanoma ; Membrane Proteins - metabolism ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchyme ; Nerve growth factor ; Osteogenesis ; Penicillin ; Population studies ; Regeneration ; Stem cells ; Surface markers ; Synovial Membrane - cytology ; Synovium ; Therapeutic applications ; Thy-1 Antigens - metabolism</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0129096-e0129096</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ogata et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Ogata et al 2015 Ogata et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-18bab13b016136ab4f0acee2e1c352b5295004ae07da36a3e561d85729cff1673</citedby><cites>FETCH-LOGICAL-c692t-18bab13b016136ab4f0acee2e1c352b5295004ae07da36a3e561d85729cff1673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459808/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459808/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26053045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wagner, Wolfgang</contributor><creatorcontrib>Ogata, Yusuke</creatorcontrib><creatorcontrib>Mabuchi, Yo</creatorcontrib><creatorcontrib>Yoshida, Mayu</creatorcontrib><creatorcontrib>Suto, Eriko Grace</creatorcontrib><creatorcontrib>Suzuki, Nobuharu</creatorcontrib><creatorcontrib>Muneta, Takeshi</creatorcontrib><creatorcontrib>Sekiya, Ichiro</creatorcontrib><creatorcontrib>Akazawa, Chihiro</creatorcontrib><title>Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. 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These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application.</description><subject>Adipocytes</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Adipogenesis</subject><subject>Arthritis</subject><subject>Biochemistry</subject><subject>Biophysics</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cartilage</subject><subject>Cartilage - physiology</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Separation</subject><subject>Cell surface</subject><subject>Clone Cells</subject><subject>Colony-Forming Units Assay</subject><subject>Cytometry</subject><subject>Data processing</subject><subject>Differentiation</subject><subject>Experiments</subject><subject>Fibroblasts</subject><subject>Flow cytometry</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Joint surgery</subject><subject>Melanoma</subject><subject>Membrane Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogata, Yusuke</au><au>Mabuchi, Yo</au><au>Yoshida, Mayu</au><au>Suto, Eriko Grace</au><au>Suzuki, Nobuharu</au><au>Muneta, Takeshi</au><au>Sekiya, Ichiro</au><au>Akazawa, Chihiro</au><au>Wagner, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-08</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0129096</spage><epage>e0129096</epage><pages>e0129096-e0129096</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR(+) THY-1 (+) MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR (+) THY-1 (+) MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26053045</pmid><doi>10.1371/journal.pone.0129096</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes Adipocytes - cytology Adipocytes - metabolism Adipogenesis Arthritis Biochemistry Biophysics Bone marrow Bone Marrow Cells - cytology Cartilage Cartilage - physiology Cell culture Cell Differentiation Cell Proliferation Cell Separation Cell surface Clone Cells Colony-Forming Units Assay Cytometry Data processing Differentiation Experiments Fibroblasts Flow cytometry Growth factors Humans Immunoglobulins Joint surgery Melanoma Membrane Proteins - metabolism Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchyme Nerve growth factor Osteogenesis Penicillin Population studies Regeneration Stem cells Surface markers Synovial Membrane - cytology Synovium Therapeutic applications Thy-1 Antigens - metabolism |
| title | Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration |
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