Dietary supplement enriched in antioxidants and omega-3 protects from progressive light-induced retinal degeneration
In the present study, we have evaluated one of the dietary supplements enriched with antioxidants and fish oil used in clinical care for patient with age-related macular degeneration. Rats were orally fed by a gastric canula daily with 0.2 ml of water or dietary supplement until they were sacrificed...
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| description | In the present study, we have evaluated one of the dietary supplements enriched with antioxidants and fish oil used in clinical care for patient with age-related macular degeneration. Rats were orally fed by a gastric canula daily with 0.2 ml of water or dietary supplement until they were sacrificed. After one week of treatment, animals were either sacrificed for lipid analysis in plasma and retina, or used for evaluation of rod-response recovery by electroretinography (ERG) followed by their sacrifice to measure rhodopsin content, or used for progressive light-induced retinal degeneration (PLIRD). For PLIRD, animals were transferred to bright cyclic light for one week. Retinal damage was quantified by ERG, histology and detection of apoptotic nuclei. Animals kept in dim-cyclic-light were processed in parallel. PLIRD induced a thinning of the outer nuclear layer and a reduction of the b-wave amplitude of the ERG in the water group. Retinal structure and function were preserved in supplemented animals. Supplement induced a significant increase in omega-3 fatty acids in plasma by 168% for eicosapentaenoic acid (EPA), 142% for docosapentaenoic acid (DPA) and 19% for docosahexaenoic acid (DHA) and a decrease in the omega-6 fatty acids, DPA by 28%. In the retina, supplement induced significant reduction of linolenic acid by 67% and an increase in EPA and DPA by 80% and 72%, respectively, associated with significant decrease in omega-6 DPA by 42%. Supplement did not affect rhodopsin content or rod-response recovery. The present data indicate that supplement rapidly modified the fatty acid content and induced an accumulation of EPA in the retina without affecting rhodopsin content or recovery. In addition, it protected the retina from oxidative stress induced by light. Therefore, this supplement might be beneficial to slow down progression of certain retinal degeneration. |
| doi_str_mv | 10.1371/journal.pone.0128395 |
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Rats were orally fed by a gastric canula daily with 0.2 ml of water or dietary supplement until they were sacrificed. After one week of treatment, animals were either sacrificed for lipid analysis in plasma and retina, or used for evaluation of rod-response recovery by electroretinography (ERG) followed by their sacrifice to measure rhodopsin content, or used for progressive light-induced retinal degeneration (PLIRD). For PLIRD, animals were transferred to bright cyclic light for one week. Retinal damage was quantified by ERG, histology and detection of apoptotic nuclei. Animals kept in dim-cyclic-light were processed in parallel. PLIRD induced a thinning of the outer nuclear layer and a reduction of the b-wave amplitude of the ERG in the water group. Retinal structure and function were preserved in supplemented animals. Supplement induced a significant increase in omega-3 fatty acids in plasma by 168% for eicosapentaenoic acid (EPA), 142% for docosapentaenoic acid (DPA) and 19% for docosahexaenoic acid (DHA) and a decrease in the omega-6 fatty acids, DPA by 28%. In the retina, supplement induced significant reduction of linolenic acid by 67% and an increase in EPA and DPA by 80% and 72%, respectively, associated with significant decrease in omega-6 DPA by 42%. Supplement did not affect rhodopsin content or rod-response recovery. The present data indicate that supplement rapidly modified the fatty acid content and induced an accumulation of EPA in the retina without affecting rhodopsin content or recovery. In addition, it protected the retina from oxidative stress induced by light. Therefore, this supplement might be beneficial to slow down progression of certain retinal degeneration.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0128395</identifier><identifier>PMID: 26042773</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Analysis ; Animals ; Antioxidants ; Antioxidants (Nutrients) ; Antioxidants - therapeutic use ; Apoptosis ; Apoptosis - radiation effects ; Biosynthetic Pathways ; Biosynthetic Pathways - radiation effects ; Damage detection ; Data recovery ; Diet ; Dietary minerals ; Dietary Supplements ; Disease ; Disease Progression ; Docosahexaenoic acid ; Eicosapentaenoic acid ; Electroretinograms ; Electroretinography ; Essential fatty acids ; Experiments ; Fatty acids ; Fatty Acids, Omega-3 ; Fatty Acids, Omega-3 - blood ; Fatty Acids, Omega-3 - therapeutic use ; Fatty Acids, Omega-6 ; Fatty Acids, Omega-6 - blood ; Female ; Fish oils ; Food ; Histology ; Laboratory animals ; Life Sciences ; Light ; Light - adverse effects ; Linolenic acid ; Lipids ; Macular degeneration ; Male ; Medical materials ; Neurons and Cognition ; Neuroprotective Agents ; Neuroprotective Agents - therapeutic use ; Nuclei ; Oils & fats ; Omega-3 fatty acids ; Oxidative stress ; Plasmalogens - metabolism ; Polyunsaturated fatty acids ; Rats, Sprague-Dawley ; Reduction ; Regeneration - radiation effects ; Retina ; Retina - pathology ; Retina - radiation effects ; Retinal Degeneration ; Retinal Degeneration - blood ; Retinal Degeneration - pathology ; Retinal Degeneration - prevention & control ; Retinal Rod Photoreceptor Cells - pathology ; Retinal Rod Photoreceptor Cells - radiation effects ; Rhodopsin ; Rhodopsin - metabolism ; Signal transduction ; Structure-function relationships ; Tubes ; Unsaturated fatty acids ; Vitamins</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0128395-e0128395</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ramchani-Ben Othman et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2015 Ramchani-Ben Othman et al 2015 Ramchani-Ben Othman et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c726t-e82fb8a31d1fd0a21f836efed1cfac5adb53f493a9adfc3bc425fdb3271933af3</citedby><cites>FETCH-LOGICAL-c726t-e82fb8a31d1fd0a21f836efed1cfac5adb53f493a9adfc3bc425fdb3271933af3</cites><orcidid>0000-0002-7092-6744</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455991/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455991/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26042773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://uca.hal.science/hal-04610218$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramchani-Ben Othman, Khaoula</creatorcontrib><creatorcontrib>Cercy, Christine</creatorcontrib><creatorcontrib>Amri, Mohamed</creatorcontrib><creatorcontrib>Doly, Michel</creatorcontrib><creatorcontrib>Ranchon-Cole, Isabelle</creatorcontrib><title>Dietary supplement enriched in antioxidants and omega-3 protects from progressive light-induced retinal degeneration</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In the present study, we have evaluated one of the dietary supplements enriched with antioxidants and fish oil used in clinical care for patient with age-related macular degeneration. Rats were orally fed by a gastric canula daily with 0.2 ml of water or dietary supplement until they were sacrificed. After one week of treatment, animals were either sacrificed for lipid analysis in plasma and retina, or used for evaluation of rod-response recovery by electroretinography (ERG) followed by their sacrifice to measure rhodopsin content, or used for progressive light-induced retinal degeneration (PLIRD). For PLIRD, animals were transferred to bright cyclic light for one week. Retinal damage was quantified by ERG, histology and detection of apoptotic nuclei. Animals kept in dim-cyclic-light were processed in parallel. PLIRD induced a thinning of the outer nuclear layer and a reduction of the b-wave amplitude of the ERG in the water group. Retinal structure and function were preserved in supplemented animals. Supplement induced a significant increase in omega-3 fatty acids in plasma by 168% for eicosapentaenoic acid (EPA), 142% for docosapentaenoic acid (DPA) and 19% for docosahexaenoic acid (DHA) and a decrease in the omega-6 fatty acids, DPA by 28%. In the retina, supplement induced significant reduction of linolenic acid by 67% and an increase in EPA and DPA by 80% and 72%, respectively, associated with significant decrease in omega-6 DPA by 42%. Supplement did not affect rhodopsin content or rod-response recovery. The present data indicate that supplement rapidly modified the fatty acid content and induced an accumulation of EPA in the retina without affecting rhodopsin content or recovery. In addition, it protected the retina from oxidative stress induced by light. Therefore, this supplement might be beneficial to slow down progression of certain retinal degeneration.</description><subject>Age</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Antioxidants - therapeutic use</subject><subject>Apoptosis</subject><subject>Apoptosis - radiation effects</subject><subject>Biosynthetic Pathways</subject><subject>Biosynthetic Pathways - radiation effects</subject><subject>Damage detection</subject><subject>Data recovery</subject><subject>Diet</subject><subject>Dietary minerals</subject><subject>Dietary Supplements</subject><subject>Disease</subject><subject>Disease Progression</subject><subject>Docosahexaenoic acid</subject><subject>Eicosapentaenoic acid</subject><subject>Electroretinograms</subject><subject>Electroretinography</subject><subject>Essential fatty acids</subject><subject>Experiments</subject><subject>Fatty acids</subject><subject>Fatty Acids, Omega-3</subject><subject>Fatty Acids, Omega-3 - blood</subject><subject>Fatty Acids, Omega-3 - therapeutic use</subject><subject>Fatty Acids, Omega-6</subject><subject>Fatty Acids, Omega-6 - blood</subject><subject>Female</subject><subject>Fish oils</subject><subject>Food</subject><subject>Histology</subject><subject>Laboratory animals</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Light - adverse effects</subject><subject>Linolenic acid</subject><subject>Lipids</subject><subject>Macular degeneration</subject><subject>Male</subject><subject>Medical materials</subject><subject>Neurons and Cognition</subject><subject>Neuroprotective Agents</subject><subject>Neuroprotective Agents - therapeutic use</subject><subject>Nuclei</subject><subject>Oils & fats</subject><subject>Omega-3 fatty acids</subject><subject>Oxidative stress</subject><subject>Plasmalogens - metabolism</subject><subject>Polyunsaturated fatty acids</subject><subject>Rats, Sprague-Dawley</subject><subject>Reduction</subject><subject>Regeneration - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramchani-Ben Othman, Khaoula</au><au>Cercy, Christine</au><au>Amri, Mohamed</au><au>Doly, Michel</au><au>Ranchon-Cole, Isabelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary supplement enriched in antioxidants and omega-3 protects from progressive light-induced retinal degeneration</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-04</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0128395</spage><epage>e0128395</epage><pages>e0128395-e0128395</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In the present study, we have evaluated one of the dietary supplements enriched with antioxidants and fish oil used in clinical care for patient with age-related macular degeneration. Rats were orally fed by a gastric canula daily with 0.2 ml of water or dietary supplement until they were sacrificed. After one week of treatment, animals were either sacrificed for lipid analysis in plasma and retina, or used for evaluation of rod-response recovery by electroretinography (ERG) followed by their sacrifice to measure rhodopsin content, or used for progressive light-induced retinal degeneration (PLIRD). For PLIRD, animals were transferred to bright cyclic light for one week. Retinal damage was quantified by ERG, histology and detection of apoptotic nuclei. Animals kept in dim-cyclic-light were processed in parallel. PLIRD induced a thinning of the outer nuclear layer and a reduction of the b-wave amplitude of the ERG in the water group. Retinal structure and function were preserved in supplemented animals. Supplement induced a significant increase in omega-3 fatty acids in plasma by 168% for eicosapentaenoic acid (EPA), 142% for docosapentaenoic acid (DPA) and 19% for docosahexaenoic acid (DHA) and a decrease in the omega-6 fatty acids, DPA by 28%. In the retina, supplement induced significant reduction of linolenic acid by 67% and an increase in EPA and DPA by 80% and 72%, respectively, associated with significant decrease in omega-6 DPA by 42%. Supplement did not affect rhodopsin content or rod-response recovery. The present data indicate that supplement rapidly modified the fatty acid content and induced an accumulation of EPA in the retina without affecting rhodopsin content or recovery. In addition, it protected the retina from oxidative stress induced by light. Therefore, this supplement might be beneficial to slow down progression of certain retinal degeneration.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26042773</pmid><doi>10.1371/journal.pone.0128395</doi><orcidid>https://orcid.org/0000-0002-7092-6744</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-06, Vol.10 (6), p.e0128395-e0128395 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1686065277 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Age Analysis Animals Antioxidants Antioxidants (Nutrients) Antioxidants - therapeutic use Apoptosis Apoptosis - radiation effects Biosynthetic Pathways Biosynthetic Pathways - radiation effects Damage detection Data recovery Diet Dietary minerals Dietary Supplements Disease Disease Progression Docosahexaenoic acid Eicosapentaenoic acid Electroretinograms Electroretinography Essential fatty acids Experiments Fatty acids Fatty Acids, Omega-3 Fatty Acids, Omega-3 - blood Fatty Acids, Omega-3 - therapeutic use Fatty Acids, Omega-6 Fatty Acids, Omega-6 - blood Female Fish oils Food Histology Laboratory animals Life Sciences Light Light - adverse effects Linolenic acid Lipids Macular degeneration Male Medical materials Neurons and Cognition Neuroprotective Agents Neuroprotective Agents - therapeutic use Nuclei Oils & fats Omega-3 fatty acids Oxidative stress Plasmalogens - metabolism Polyunsaturated fatty acids Rats, Sprague-Dawley Reduction Regeneration - radiation effects Retina Retina - pathology Retina - radiation effects Retinal Degeneration Retinal Degeneration - blood Retinal Degeneration - pathology Retinal Degeneration - prevention & control Retinal Rod Photoreceptor Cells - pathology Retinal Rod Photoreceptor Cells - radiation effects Rhodopsin Rhodopsin - metabolism Signal transduction Structure-function relationships Tubes Unsaturated fatty acids Vitamins |
| title | Dietary supplement enriched in antioxidants and omega-3 protects from progressive light-induced retinal degeneration |
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