Case report: A retrospective serological analysis indicating human exposure to tick-borne relapsing fever spirochetes in Texas

The amplicon was cloned into the pET102 expression vector, and the absence of nucleotide errors was confirmed by sequencing analysis using Vector NTI Advanced 11.5 software (Life Technologies). rGlpQ was produced as a six-histidine and thioredoxin fusion protein and purified by immobilized metal affinity chromatography using HisTrap FF Crude columns precharged with Ni2+ (GE Healthcare Life Sciences, Pittsburgh, Pennsylvania, US). [...]O. turicata is rarely found on the host, and a full blood meal is not required for transmission and infection [14]. [...]mammalian hosts supporting the maintenance of B. turicatae in nature are not completely known.