Replicative DNA polymerase [delta] but not [epsilon] proofreads errors in cis and in trans

It is now well established that in yeast, and likely most eukaryotic organisms, initial DNA replication of the leading strand is by DNA polymerase [epsilon] and of the lagging strand by DNA polymerase [delta]. However, the role of Pol [delta] in replication of the leading strand is uncertain. In this work, we use a reporter system in Saccharomyces cerevisiae to measure mutation rates at specific base pairs in order to determine the effect of heterozygous or homozygous proofreading-defective mutants of either Pol [epsilon] or Pol [delta] in diploid strains. We find that wild-type Pol [epsilon] molecules cannot proofread errors created by proofreading-defective Pol [epsilon] molecules, whereas Pol [delta] can not only proofread errors created by proofreading-defective Pol [delta] molecules, but can also proofread errors created by Pol [epsilon]-defective molecules. These results suggest that any interruption in DNA synthesis on the leading strand is likely to result in completion by Pol [delta] and also explain the higher mutation rates observed in Pol [delta]proofreading mutants compared to Pol [epsilon]-proofreading defective mutants. For strains reverting via AT [right arrow] GC, TA [right arrow] GC, CG [right arrow] AT, and GC [right arrow] AT mutations, we find in addition a strong effect of gene orientation on mutation rate in proofreading-defective strains and demonstrate that much of this orientation dependence is due to differential efficiencies of mispair elongation. We also find that a 3'-terminal 8 oxoG, unlike a 3'-terminal G, is efficiently extended opposite an A and is not subject to proofreading. Proofreading mutations have been shown to result in tumor formation in both mice and humans; the results presented here can help explain the properties exhibited by those proofreading mutants.