The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori
Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was...
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| description | Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio ≥ 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre. |
| doi_str_mv | 10.1371/journal.pone.0128211 |
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While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio ≥ 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0128211</identifier><identifier>PMID: 26030868</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Base Sequence ; Biochemistry ; Bombyx ; Bombyx - embryology ; Bombyx - genetics ; Bombyx - metabolism ; Bombyx mori ; Cloning ; Color ; Eggs ; Embryo, Nonmammalian - embryology ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; Embryos ; Female ; Gene expression ; Gene Expression Regulation, Developmental ; Gene sequencing ; Genes ; Genetic analysis ; Genetic engineering ; Genomes ; Germplasm ; Heredity ; Identification ; Incubation ; Insect Proteins - genetics ; Lethality ; Molecular biology ; Molecular Sequence Data ; Mutants ; Mutation ; Ovum - metabolism ; Pigmentation - genetics ; Science ; Sericulture ; Structural analysis ; Studies ; Transcription</subject><ispartof>PloS one, 2015-06, Vol.10 (6), p.e0128211-e0128211</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Chen et al 2015 Chen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-98a326b3339bea88735c9fdeb981ec99bae4d80533465beeb7cd544722b47583</citedby><cites>FETCH-LOGICAL-c692t-98a326b3339bea88735c9fdeb981ec99bae4d80533465beeb7cd544722b47583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452371/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452371/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26030868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Anli</creatorcontrib><creatorcontrib>Liao, Pengfei</creatorcontrib><creatorcontrib>Li, Qiongyan</creatorcontrib><creatorcontrib>Zhao, Qiaoling</creatorcontrib><creatorcontrib>Yang, Weike</creatorcontrib><creatorcontrib>Zhu, Shuifen</creatorcontrib><creatorcontrib>Wu, Fang</creatorcontrib><creatorcontrib>He, Rongfan</creatorcontrib><creatorcontrib>Dong, Zhanpeng</creatorcontrib><creatorcontrib>Huang, Ping</creatorcontrib><title>The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio ≥ 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre.</description><subject>Analysis</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Bombyx</subject><subject>Bombyx - embryology</subject><subject>Bombyx - genetics</subject><subject>Bombyx - metabolism</subject><subject>Bombyx mori</subject><subject>Cloning</subject><subject>Color</subject><subject>Eggs</subject><subject>Embryo, Nonmammalian - embryology</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic analysis</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Germplasm</subject><subject>Heredity</subject><subject>Identification</subject><subject>Incubation</subject><subject>Insect Proteins - genetics</subject><subject>Lethality</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Ovum - metabolism</subject><subject>Pigmentation - genetics</subject><subject>Science</subject><subject>Sericulture</subject><subject>Structural analysis</subject><subject>Studies</subject><subject>Transcription</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBYQkIg0eLETmLfIJVpg0qFSdu0W8txTloPJy62s613_HTcNZsWtAvkC3895z0f9kmS1ymepqRMP1_a3nXSTNe2gylOM5al6ZNkP-UkmxQZJk8frPeSF95fYpwTVhTPk72swASzgu0nf85XgM6C61XonTToQjotg7Ydmns0896quIUaXeuwQiGyR23lNrbTCi0grKTRYYNsgyT6aa_AoNPIHi2X6EcfZBfQcb_R3cQ42DJn2vy6tq79hL7attrcoNY6_TJ51kjj4dUwHyTnx0fnh98ni5Nv88PZYqIKnoUJZ5JkRUUI4RVIxkqSK97UUHGWguK8kkBrFhMktMgrgKpUdU5pmWUVLXNGDpK3O9m1sV4MtfMiLRjlPCcljsR8R9RWXoq10610G2GlFrcH1i2FdEErA4KXmKeS4lzVGcUllsCagtcEOMWKMhq1vgze-qqFWkEXYnFHouObTq_E0l4JSvMsPm4U-DAIOPu7Bx9Eq70CY2QHtr-NOy_LIe53_6CPZzdQSxkT0F1jo1-1FRUzmhFG8rwgkZo-QsVRQ6tV_GiNjucjg48jg8gEuAlL2Xsv5men_8-eXIzZ9w_YFUgTVt6afvsz_RikO1A5672D5r7IKRbbPrmrhtj2iRj6JJq9efhA90Z3jUH-ApG7DLU</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Chen, Anli</creator><creator>Liao, Pengfei</creator><creator>Li, Qiongyan</creator><creator>Zhao, Qiaoling</creator><creator>Yang, Weike</creator><creator>Zhu, Shuifen</creator><creator>Wu, Fang</creator><creator>He, Rongfan</creator><creator>Dong, Zhanpeng</creator><creator>Huang, Ping</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150601</creationdate><title>The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori</title><author>Chen, Anli ; Liao, Pengfei ; Li, Qiongyan ; Zhao, Qiaoling ; Yang, Weike ; Zhu, Shuifen ; Wu, Fang ; He, Rongfan ; Dong, Zhanpeng ; Huang, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-98a326b3339bea88735c9fdeb981ec99bae4d80533465beeb7cd544722b47583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Bombyx</topic><topic>Bombyx - embryology</topic><topic>Bombyx - genetics</topic><topic>Bombyx - metabolism</topic><topic>Bombyx mori</topic><topic>Cloning</topic><topic>Color</topic><topic>Eggs</topic><topic>Embryo, Nonmammalian - embryology</topic><topic>Embryogenesis</topic><topic>Embryonic development</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic analysis</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Germplasm</topic><topic>Heredity</topic><topic>Identification</topic><topic>Incubation</topic><topic>Insect Proteins - genetics</topic><topic>Lethality</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Ovum - metabolism</topic><topic>Pigmentation - genetics</topic><topic>Science</topic><topic>Sericulture</topic><topic>Structural analysis</topic><topic>Studies</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Anli</creatorcontrib><creatorcontrib>Liao, Pengfei</creatorcontrib><creatorcontrib>Li, Qiongyan</creatorcontrib><creatorcontrib>Zhao, Qiaoling</creatorcontrib><creatorcontrib>Yang, Weike</creatorcontrib><creatorcontrib>Zhu, Shuifen</creatorcontrib><creatorcontrib>Wu, Fang</creatorcontrib><creatorcontrib>He, Rongfan</creatorcontrib><creatorcontrib>Dong, Zhanpeng</creatorcontrib><creatorcontrib>Huang, Ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Anli</au><au>Liao, Pengfei</au><au>Li, Qiongyan</au><au>Zhao, Qiaoling</au><au>Yang, Weike</au><au>Zhu, Shuifen</au><au>Wu, Fang</au><au>He, Rongfan</au><au>Dong, Zhanpeng</au><au>Huang, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>10</volume><issue>6</issue><spage>e0128211</spage><epage>e0128211</epage><pages>e0128211-e0128211</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B. mori. This mutant features red-colored eggs and embryonic lethality. Genetic analysis showed that Fuyin-lre follows recessive inheritance, with the red egg gene re governing the egg color, and the embryonic lethality of Fuyin-lre may be caused by mutations of other genes closely linked to re. Digital gene expression (DGE) was employed to compare the transcription profiles of Fuyin and Fuyin-lre eggs after 24 and 48 h of incubation. A total of 48 differentially expressed genes followed the same expression patterns in both groups at both time points (FDR < 0.01 and log 2 Ratio ≥ 1). Further analyses indicated that 8 out of the 48 genes (including re) were closely linked to re. These 8 genes were highly expressed in wild-type Fuyin and the red egg mutant re but showed nearly absent expression in Fuyin-lre. Sequencing of the re gene confirmed that the re gene itself does not induce embryonic lethality, and structure analysis showed that the structural variation of the region where the 8 genes were located may be associated with the embryonic lethality of Fuyin-lre. The present work provides a good foundation for future studies on the mechanism of embryonic lethality and embryonic development in Fuyin-lre.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26030868</pmid><doi>10.1371/journal.pone.0128211</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1684995370 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Analysis Animals Base Sequence Biochemistry Bombyx Bombyx - embryology Bombyx - genetics Bombyx - metabolism Bombyx mori Cloning Color Eggs Embryo, Nonmammalian - embryology Embryogenesis Embryonic development Embryonic growth stage Embryos Female Gene expression Gene Expression Regulation, Developmental Gene sequencing Genes Genetic analysis Genetic engineering Genomes Germplasm Heredity Identification Incubation Insect Proteins - genetics Lethality Molecular biology Molecular Sequence Data Mutants Mutation Ovum - metabolism Pigmentation - genetics Science Sericulture Structural analysis Studies Transcription |
| title | The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T17%3A47%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Structural%20Variation%20Is%20Associated%20with%20the%20Embryonic%20Lethality%20of%20a%20Novel%20Red%20Egg%20Mutant%20Fuyin-lre%20of%20Silkworm,%20Bombyx%20mori&rft.jtitle=PloS%20one&rft.au=Chen,%20Anli&rft.date=2015-06-01&rft.volume=10&rft.issue=6&rft.spage=e0128211&rft.epage=e0128211&rft.pages=e0128211-e0128211&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0128211&rft_dat=%3Cgale_plos_%3EA423835563%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1684995370&rft_id=info:pmid/26030868&rft_galeid=A423835563&rft_doaj_id=oai_doaj_org_article_97091a405cd24070ae8f69d3e940c484&rfr_iscdi=true |