Detection of circulating parasite-derived microRNAs in filarial infections
Filarial nematodes cause chronic and profoundly debilitating diseases in both humans and animals. Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investiga...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2014-07, Vol.8 (7), p.e2971-e2971 |
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| creator | Tritten, Lucienne Burkman, Erica Moorhead, Andrew Satti, Mohammed Geary, James Mackenzie, Charles Geary, Timothy |
| description | Filarial nematodes cause chronic and profoundly debilitating diseases in both humans and animals. Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investigated the presence of circulating miRNAs released by filarial nematodes into the host bloodstream. miRNA deep-sequencing combined with bioinformatics revealed over 200 mature miRNA sequences of potential nematode origin in Dirofilaria immitis-infected dog plasma in two independent analyses, and 21 in Onchocerca volvulus-infected human serum. Total RNA obtained from D. immitis-infected dog plasma was subjected to stem-loop RT-qPCR assays targeting two detected miRNA candidates, miR-71 and miR-34. Additionally, Brugia pahangi-infected dog samples were included in the analysis, as these miRNAs were previously detected in extracts prepared from this species. The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream. |
| doi_str_mv | 10.1371/journal.pntd.0002971 |
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Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investigated the presence of circulating miRNAs released by filarial nematodes into the host bloodstream. miRNA deep-sequencing combined with bioinformatics revealed over 200 mature miRNA sequences of potential nematode origin in Dirofilaria immitis-infected dog plasma in two independent analyses, and 21 in Onchocerca volvulus-infected human serum. Total RNA obtained from D. immitis-infected dog plasma was subjected to stem-loop RT-qPCR assays targeting two detected miRNA candidates, miR-71 and miR-34. Additionally, Brugia pahangi-infected dog samples were included in the analysis, as these miRNAs were previously detected in extracts prepared from this species. The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0002971</identifier><identifier>PMID: 25033073</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens ; Bioinformatics ; Biology and life sciences ; Brugia ; Candidates ; Diagnosis ; Dirofilaria immitis - genetics ; Dirofilariasis - parasitology ; Dogs ; Filariasis ; Gene expression ; Host-Parasite Interactions ; Infections ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; Nematoda ; Onchocerca volvulus ; Parasites ; Physiological aspects ; Plasma ; RNA, Helminth - blood ; Tropical diseases</subject><ispartof>PLoS neglected tropical diseases, 2014-07, Vol.8 (7), p.e2971-e2971</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Tritten et al 2014 Tritten et al</rights><rights>2014 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Tritten L, Burkman E, Moorhead A, Satti M, Geary J, Mackenzie C, et al. (2014) Detection of Circulating Parasite-Derived MicroRNAs in Filarial Infections. PLoS Negl Trop Dis 8(7): e2971. doi:10.1371/journal.pntd.0002971</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c695t-62d5308550beb6ffd7d8e200c5f6504647acdfbfccafa10f6644a31efc7edaff3</citedby><cites>FETCH-LOGICAL-c695t-62d5308550beb6ffd7d8e200c5f6504647acdfbfccafa10f6644a31efc7edaff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102413/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102413/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25033073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nutman, Thomas B.</contributor><creatorcontrib>Tritten, Lucienne</creatorcontrib><creatorcontrib>Burkman, Erica</creatorcontrib><creatorcontrib>Moorhead, Andrew</creatorcontrib><creatorcontrib>Satti, Mohammed</creatorcontrib><creatorcontrib>Geary, James</creatorcontrib><creatorcontrib>Mackenzie, Charles</creatorcontrib><creatorcontrib>Geary, Timothy</creatorcontrib><title>Detection of circulating parasite-derived microRNAs in filarial infections</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Filarial nematodes cause chronic and profoundly debilitating diseases in both humans and animals. Applications of novel technology are providing unprecedented opportunities to improve diagnosis and our understanding of the molecular basis for host-parasite interactions. As a first step, we investigated the presence of circulating miRNAs released by filarial nematodes into the host bloodstream. miRNA deep-sequencing combined with bioinformatics revealed over 200 mature miRNA sequences of potential nematode origin in Dirofilaria immitis-infected dog plasma in two independent analyses, and 21 in Onchocerca volvulus-infected human serum. Total RNA obtained from D. immitis-infected dog plasma was subjected to stem-loop RT-qPCR assays targeting two detected miRNA candidates, miR-71 and miR-34. Additionally, Brugia pahangi-infected dog samples were included in the analysis, as these miRNAs were previously detected in extracts prepared from this species. The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream.</description><subject>Animals</subject><subject>Antigens</subject><subject>Bioinformatics</subject><subject>Biology and life sciences</subject><subject>Brugia</subject><subject>Candidates</subject><subject>Diagnosis</subject><subject>Dirofilaria immitis - genetics</subject><subject>Dirofilariasis - parasitology</subject><subject>Dogs</subject><subject>Filariasis</subject><subject>Gene expression</subject><subject>Host-Parasite Interactions</subject><subject>Infections</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>Nematoda</subject><subject>Onchocerca volvulus</subject><subject>Parasites</subject><subject>Physiological aspects</subject><subject>Plasma</subject><subject>RNA, Helminth - blood</subject><subject>Tropical diseases</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEYhQdRbK3-A9EBoXiza76TuRGW1o9KURC9DtnkzW6W7GSbzBT892Y_WnbBC5mLCZPnnEzec5rmNUZTTCX-sEpj7k2cbvrBTRFCpJP4SXOOO8onRFL-9Gh91rwoZYUQ77jCz5szwhGlSNLz5ts1DGCHkPo2-daGbMdohtAv2o3JpoQBJg5yuAfXroPN6ef3WWlD3_oQTQ4m1rXf68vL5pk3scCrw_ui-f3506-rr5PbH19urma3Eys6PkwEcZwixTmaw1x476RTQBCy3AuOmGDSWOfn3lrjDUZeCMYMxeCtBGe8pxfN273vJqaiD2MoGgvFBFVE0Erc7AmXzEpvclib_EcnE_TuQ8oLbfIQbARNoHNYEqsUxgwL6IwUnZBzZTDv_M7r4-G0cb4GZ6Efsoknpqc7fVjqRbrXDCPC8Nbg_cEgp7sRyqDXoViI0fSQxvrfXCCMqOz-B2WC4A4zWdF3e3Rh6i1qCKkebre4nlFFKVFMqUpN_0HVx0FNM_VQY4RTweWRYAkmDsuS4rgL-BRke7B2opQM_nEiGOltPx-C0dt-6kM_q-zN8TQfRQ-FpH8BbxPiXg</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Tritten, Lucienne</creator><creator>Burkman, Erica</creator><creator>Moorhead, Andrew</creator><creator>Satti, Mohammed</creator><creator>Geary, James</creator><creator>Mackenzie, Charles</creator><creator>Geary, Timothy</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140701</creationdate><title>Detection of circulating parasite-derived microRNAs in filarial infections</title><author>Tritten, Lucienne ; 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The presence of miR-71 and miR-34 discriminated infected samples (both species) from uninfected samples, in which no specific miRNA amplification occurred. However, absolute miRNA copy numbers were not significantly correlated with microfilaraemia for either parasite. This may be due to the imprecision of mf counts to estimate infection intensity or to miRNA contributions from the unknown number of adult worms present. Nonetheless, parasite-derived circulating miRNAs are found in plasma or serum even for those species that do not live in the bloodstream.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25033073</pmid><doi>10.1371/journal.pntd.0002971</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigens Bioinformatics Biology and life sciences Brugia Candidates Diagnosis Dirofilaria immitis - genetics Dirofilariasis - parasitology Dogs Filariasis Gene expression Host-Parasite Interactions Infections MicroRNA MicroRNAs MicroRNAs - blood Nematoda Onchocerca volvulus Parasites Physiological aspects Plasma RNA, Helminth - blood Tropical diseases |
| title | Detection of circulating parasite-derived microRNAs in filarial infections |
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