Quantitative Measurement of GPCR Endocytosis via Pulse-Chase Covalent Labeling

G protein-coupled receptors (GPCRs) play a critical role in many physiological systems and represent one of the largest families of signal-transducing receptors. The number of GPCRs at the cell surface regulates cellular responsiveness to their cognate ligands, and the number of GPCRs, in turn, is d...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2015-05, Vol.10 (5), p.e0129394-e0129394
Hauptverfasser:	Kumagai, Hidetoshi, Ikeda, Yuichi, Motozawa, Yoshihiro, Fujishiro, Mitsuhiro, Okamura, Tomohisa, Fujio, Keishi, Okazaki, Hiroaki, Nomura, Seitaro, Takeda, Norifumi, Harada, Mutsuo, Toko, Haruhiro, Takimoto, Eiki, Akazawa, Hiroshi, Morita, Hiroyuki, Suzuki, Jun-ichi, Yamazaki, Tsutomu, Yamamoto, Kazuhiko, Komuro, Issei, Yanagisawa, Masashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological Assay Biotin Biotin - chemistry Cell Membrane - chemistry Cell Membrane - metabolism Cell surface Chemical compounds CHO Cells Covalence Cricetulus Desensitization Endocytosis Endocytosis - genetics Fluorescence Fluorescent Dyes - chemistry Fluorophores G protein-coupled receptors G proteins Gene Expression Genetic aspects Genetic engineering Genetically modified organisms Hydrolase Hydrolases - genetics Hydrolases - metabolism Internalization Labeling Labelling Ligands Measurement Medicine Molecular Sequence Data Narcotics Orexin Receptors - genetics Orexin Receptors - metabolism Pharmacology Physicians Physiology Plasmids - chemistry Plasmids - genetics Polyethylene glycol Protein Engineering Protein Transport Proteins Receptors Receptors, Bombesin - genetics Receptors, Bombesin - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Rheumatology Signal Transduction Signaling Staining and Labeling - methods Transduction, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!