CEMP1 Induces Transformation in Human Gingival Fibroblasts
Cementum Protein 1 (CEMP1) is a key regulator of cementogenesis. CEMP1 promotes cell attachment, differentiation, deposition rate, composition, and morphology of hydroxyapatite crystals formed by human cementoblastic cells. Its expression is restricted to cementoblasts and progenitor cell subpopulat...
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| description | Cementum Protein 1 (CEMP1) is a key regulator of cementogenesis. CEMP1 promotes cell attachment, differentiation, deposition rate, composition, and morphology of hydroxyapatite crystals formed by human cementoblastic cells. Its expression is restricted to cementoblasts and progenitor cell subpopulations present in the periodontal ligament. CEMP1 transfection into non-osteogenic cells such as adult human gingival fibroblasts results in differentiation of these cells into a "mineralizing" cell phenotype. Other studies have shown evidence that CEMP1 could have a therapeutic potential for the treatment of bone defects and regeneration of other mineralized tissues. To better understand CEMP1's biological effects in vitro we investigated the consequences of its expression in human gingival fibroblasts (HGF) growing in non-mineralizing media by comparing gene expression profiles. We identified several mRNAs whose expression is modified by CEMP1 induction in HGF cells. Enrichment analysis showed that several of these newly expressed genes are involved in oncogenesis. Our results suggest that CEMP1 causes the transformation of HGF and NIH3T3 cells. CEMP1 is overexpressed in cancer cell lines. We also determined that the region spanning the CEMP1 locus is commonly amplified in a variety of cancers, and finally we found significant overexpression of CEMP1 in leukemia, cervix, breast, prostate and lung cancer. Our findings suggest that CEMP1 exerts modulation of a number of cellular genes, cellular development, cellular growth, cell death, and cell cycle, and molecules associated with cancer. |
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CEMP1 promotes cell attachment, differentiation, deposition rate, composition, and morphology of hydroxyapatite crystals formed by human cementoblastic cells. Its expression is restricted to cementoblasts and progenitor cell subpopulations present in the periodontal ligament. CEMP1 transfection into non-osteogenic cells such as adult human gingival fibroblasts results in differentiation of these cells into a "mineralizing" cell phenotype. Other studies have shown evidence that CEMP1 could have a therapeutic potential for the treatment of bone defects and regeneration of other mineralized tissues. To better understand CEMP1's biological effects in vitro we investigated the consequences of its expression in human gingival fibroblasts (HGF) growing in non-mineralizing media by comparing gene expression profiles. We identified several mRNAs whose expression is modified by CEMP1 induction in HGF cells. Enrichment analysis showed that several of these newly expressed genes are involved in oncogenesis. Our results suggest that CEMP1 causes the transformation of HGF and NIH3T3 cells. CEMP1 is overexpressed in cancer cell lines. We also determined that the region spanning the CEMP1 locus is commonly amplified in a variety of cancers, and finally we found significant overexpression of CEMP1 in leukemia, cervix, breast, prostate and lung cancer. Our findings suggest that CEMP1 exerts modulation of a number of cellular genes, cellular development, cellular growth, cell death, and cell cycle, and molecules associated with cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0127286</identifier><identifier>PMID: 26011628</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biocompatibility ; Biological effects ; Biomedical materials ; Bone and Bones - metabolism ; Bone and Bones - pathology ; Bone growth ; Breast cancer ; Cancer ; Cancer cells ; Carcinogenesis - genetics ; Carcinogenesis - metabolism ; Cell adhesion ; Cell cycle ; Cell death ; Cell Differentiation - genetics ; Cell Line ; Cell Line, Tumor ; Cell Proliferation - genetics ; Cell Transformation, Neoplastic - genetics ; Cell Transformation, Neoplastic - metabolism ; Cell Transformation, Neoplastic - pathology ; Cements ; Cementum ; Cervix ; Crystal defects ; Crystals ; Cytology ; Dentistry ; Differentiation (biology) ; Fibroblasts ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Gene expression ; Genes ; Genetic aspects ; Genetic transformation ; Genomes ; Gingiva ; Gingiva - metabolism ; Gingiva - pathology ; Growth factors ; Health aspects ; Humans ; Hybridization ; Hydroxyapatite ; Kinases ; Leukemia ; Lung cancer ; Lung diseases ; Mice ; NIH 3T3 Cells ; Periodontal ligament ; Progenitor cells ; Prostate cancer ; Proteins ; Proteins - genetics ; Proteins - metabolism ; Regeneration ; Regeneration - genetics ; RNA, Messenger - genetics ; Rodents ; Stem cells ; Studies ; Subpopulations ; Surgical implants ; Tissues ; Transcriptome - genetics ; Transfection ; Transformation ; Tumor cell lines ; Tumorigenesis</subject><ispartof>PloS one, 2015-05, Vol.10 (5), p.e0127286-e0127286</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Bermúdez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Bermúdez et al 2015 Bermúdez et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c6287dd45cf35d3e7eb119b99c34c80c8ccc42b2a64fa8d48ba1f0f0f5518b563</citedby><cites>FETCH-LOGICAL-c692t-c6287dd45cf35d3e7eb119b99c34c80c8ccc42b2a64fa8d48ba1f0f0f5518b563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444236/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444236/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26011628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Trackman, Philip C.</contributor><creatorcontrib>Bermúdez, Mercedes</creatorcontrib><creatorcontrib>Imaz-Rosshandler, Ivan</creatorcontrib><creatorcontrib>Rangel-Escareño, Claudia</creatorcontrib><creatorcontrib>Zeichner-David, Margarita</creatorcontrib><creatorcontrib>Arzate, Higinio</creatorcontrib><creatorcontrib>Mercado-Celis, Gabriela E</creatorcontrib><title>CEMP1 Induces Transformation in Human Gingival Fibroblasts</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cementum Protein 1 (CEMP1) is a key regulator of cementogenesis. CEMP1 promotes cell attachment, differentiation, deposition rate, composition, and morphology of hydroxyapatite crystals formed by human cementoblastic cells. Its expression is restricted to cementoblasts and progenitor cell subpopulations present in the periodontal ligament. CEMP1 transfection into non-osteogenic cells such as adult human gingival fibroblasts results in differentiation of these cells into a "mineralizing" cell phenotype. Other studies have shown evidence that CEMP1 could have a therapeutic potential for the treatment of bone defects and regeneration of other mineralized tissues. To better understand CEMP1's biological effects in vitro we investigated the consequences of its expression in human gingival fibroblasts (HGF) growing in non-mineralizing media by comparing gene expression profiles. We identified several mRNAs whose expression is modified by CEMP1 induction in HGF cells. Enrichment analysis showed that several of these newly expressed genes are involved in oncogenesis. Our results suggest that CEMP1 causes the transformation of HGF and NIH3T3 cells. CEMP1 is overexpressed in cancer cell lines. We also determined that the region spanning the CEMP1 locus is commonly amplified in a variety of cancers, and finally we found significant overexpression of CEMP1 in leukemia, cervix, breast, prostate and lung cancer. 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genetics</subject><subject>Proteins - metabolism</subject><subject>Regeneration</subject><subject>Regeneration - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Subpopulations</subject><subject>Surgical implants</subject><subject>Tissues</subject><subject>Transcriptome - genetics</subject><subject>Transfection</subject><subject>Transformation</subject><subject>Tumor cell lines</subject><subject>Tumorigenesis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhou4uOvoPxAtCKIXM-a7qRfCMuzHwMqKrt6GNE1nMqTJmLSL_ntTp7tMl72wgSYkz3nPOcmbZa8gWEBcwI9b3wcn7WLnnV4AiArE2ZPsBJYYzRkC-OnB-jh7HuMWAIo5Y8-yY8QAhAzxk-zT8uzLV5ivXN0rHfObIF1sfGhlZ7zLjcsv-1a6_MK4tbmVNj83VfCVlbGLL7KjRtqoX47zLPtxfnazvJxfXV-slqdXc8VK1KU_4kVdE6oaTGusC11BWFZlqTBRHCiulCKoQpKRRvKa8ErCBqRBKeQVZXiWvdnr7qyPYmw7Csg4xgVgsEjEak_UXm7FLphWhj_CSyP-bfiwFjJ0RlktGC00p4SWkJdDSVyBAoJSM8wxkQ1IWp_HbH3V6lpp1wVpJ6LTE2c2Yu1vBUkfwkO570eB4H_1OnaiNVFpa6XTvt_XXVBWQpLQtw_Qx7sbqbVMDRjX-JRXDaLilGBEIaMp8SxbPEKlUevWqOSRxqT9ScCHSUBiOv27W8s-RrH6_u3_2eufU_bdAbvR0nab6G0_GCpOQbIHVfAxBt3cXzIEYrD43W2IweJitHgKe334QPdBd57GfwEvQfOd</recordid><startdate>20150526</startdate><enddate>20150526</enddate><creator>Bermúdez, Mercedes</creator><creator>Imaz-Rosshandler, Ivan</creator><creator>Rangel-Escareño, Claudia</creator><creator>Zeichner-David, Margarita</creator><creator>Arzate, Higinio</creator><creator>Mercado-Celis, Gabriela E</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150526</creationdate><title>CEMP1 Induces Transformation in Human Gingival Fibroblasts</title><author>Bermúdez, Mercedes ; Imaz-Rosshandler, Ivan ; Rangel-Escareño, Claudia ; Zeichner-David, Margarita ; Arzate, Higinio ; Mercado-Celis, Gabriela E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-c6287dd45cf35d3e7eb119b99c34c80c8ccc42b2a64fa8d48ba1f0f0f5518b563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biological effects</topic><topic>Biomedical materials</topic><topic>Bone and Bones - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bermúdez, Mercedes</au><au>Imaz-Rosshandler, Ivan</au><au>Rangel-Escareño, Claudia</au><au>Zeichner-David, Margarita</au><au>Arzate, Higinio</au><au>Mercado-Celis, Gabriela E</au><au>Trackman, Philip C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CEMP1 Induces Transformation in Human Gingival Fibroblasts</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-05-26</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>e0127286</spage><epage>e0127286</epage><pages>e0127286-e0127286</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cementum Protein 1 (CEMP1) is a key regulator of cementogenesis. CEMP1 promotes cell attachment, differentiation, deposition rate, composition, and morphology of hydroxyapatite crystals formed by human cementoblastic cells. Its expression is restricted to cementoblasts and progenitor cell subpopulations present in the periodontal ligament. CEMP1 transfection into non-osteogenic cells such as adult human gingival fibroblasts results in differentiation of these cells into a "mineralizing" cell phenotype. Other studies have shown evidence that CEMP1 could have a therapeutic potential for the treatment of bone defects and regeneration of other mineralized tissues. To better understand CEMP1's biological effects in vitro we investigated the consequences of its expression in human gingival fibroblasts (HGF) growing in non-mineralizing media by comparing gene expression profiles. We identified several mRNAs whose expression is modified by CEMP1 induction in HGF cells. Enrichment analysis showed that several of these newly expressed genes are involved in oncogenesis. Our results suggest that CEMP1 causes the transformation of HGF and NIH3T3 cells. CEMP1 is overexpressed in cancer cell lines. We also determined that the region spanning the CEMP1 locus is commonly amplified in a variety of cancers, and finally we found significant overexpression of CEMP1 in leukemia, cervix, breast, prostate and lung cancer. Our findings suggest that CEMP1 exerts modulation of a number of cellular genes, cellular development, cellular growth, cell death, and cell cycle, and molecules associated with cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26011628</pmid><doi>10.1371/journal.pone.0127286</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1683370617 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Biocompatibility Biological effects Biomedical materials Bone and Bones - metabolism Bone and Bones - pathology Bone growth Breast cancer Cancer Cancer cells Carcinogenesis - genetics Carcinogenesis - metabolism Cell adhesion Cell cycle Cell death Cell Differentiation - genetics Cell Line Cell Line, Tumor Cell Proliferation - genetics Cell Transformation, Neoplastic - genetics Cell Transformation, Neoplastic - metabolism Cell Transformation, Neoplastic - pathology Cements Cementum Cervix Crystal defects Crystals Cytology Dentistry Differentiation (biology) Fibroblasts Fibroblasts - metabolism Fibroblasts - pathology Gene expression Genes Genetic aspects Genetic transformation Genomes Gingiva Gingiva - metabolism Gingiva - pathology Growth factors Health aspects Humans Hybridization Hydroxyapatite Kinases Leukemia Lung cancer Lung diseases Mice NIH 3T3 Cells Periodontal ligament Progenitor cells Prostate cancer Proteins Proteins - genetics Proteins - metabolism Regeneration Regeneration - genetics RNA, Messenger - genetics Rodents Stem cells Studies Subpopulations Surgical implants Tissues Transcriptome - genetics Transfection Transformation Tumor cell lines Tumorigenesis |
| title | CEMP1 Induces Transformation in Human Gingival Fibroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T19%3A21%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CEMP1%20Induces%20Transformation%20in%20Human%20Gingival%20Fibroblasts&rft.jtitle=PloS%20one&rft.au=Berm%C3%BAdez,%20Mercedes&rft.date=2015-05-26&rft.volume=10&rft.issue=5&rft.spage=e0127286&rft.epage=e0127286&rft.pages=e0127286-e0127286&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0127286&rft_dat=%3Cgale_plos_%3EA432516542%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1683370617&rft_id=info:pmid/26011628&rft_galeid=A432516542&rft_doaj_id=oai_doaj_org_article_657e8545918945cf8c07109e63834af0&rfr_iscdi=true |