Interaction Effects of Light, Temperature and Nutrient Limitations (N, P and Si) on Growth, Stoichiometry and Photosynthetic Parameters of the Cold-Water Diatom Chaetoceros wighamii
Light (20-450 μmol photons m(-2) s(-1)), temperature (3-11 °C) and inorganic nutrient composition (nutrient replete and N, P and Si limitation) were manipulated to study their combined influence on growth, stoichiometry (C:N:P:Chl a) and primary production of the cold water diatom Chaetoceros wigham...
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| description | Light (20-450 μmol photons m(-2) s(-1)), temperature (3-11 °C) and inorganic nutrient composition (nutrient replete and N, P and Si limitation) were manipulated to study their combined influence on growth, stoichiometry (C:N:P:Chl a) and primary production of the cold water diatom Chaetoceros wighamii. During exponential growth, the maximum growth rate (~0.8 d(-1)) was observed at high temperature and light; at 3 °C the growth rate was ~30% lower under similar light conditions. The interaction effect of light and temperature were clearly visible from growth and cellular stoichiometry. The average C:N:P molar ratio was 80:13:1 during exponential growth, but the range, due to different light acclimation, was widest at the lowest temperature, reaching very low C:P (~50) and N:P ratios (~8) at low light and temperature. The C:Chl a ratio had also a wider range at the lowest temperature during exponential growth, ranging 16-48 (weight ratio) at 3 °C compared with 17-33 at 11 °C. During exponential growth, there was no clear trend in the Chl a normalized, initial slope (α*) of the photosynthesis-irradiance (PE) curve, but the maximum photosynthetic production (P(m)) was highest for cultures acclimated to the highest light and temperature. During the stationary growth phase, the stoichiometric relationship depended on the limiting nutrient, but with generally increasing C:N:P ratio. The average photosynthetic quotient (PQ) during exponential growth was 1.26 but decreased to |
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During exponential growth, the maximum growth rate (~0.8 d(-1)) was observed at high temperature and light; at 3 °C the growth rate was ~30% lower under similar light conditions. The interaction effect of light and temperature were clearly visible from growth and cellular stoichiometry. The average C:N:P molar ratio was 80:13:1 during exponential growth, but the range, due to different light acclimation, was widest at the lowest temperature, reaching very low C:P (~50) and N:P ratios (~8) at low light and temperature. The C:Chl a ratio had also a wider range at the lowest temperature during exponential growth, ranging 16-48 (weight ratio) at 3 °C compared with 17-33 at 11 °C. During exponential growth, there was no clear trend in the Chl a normalized, initial slope (α*) of the photosynthesis-irradiance (PE) curve, but the maximum photosynthetic production (P(m)) was highest for cultures acclimated to the highest light and temperature. During the stationary growth phase, the stoichiometric relationship depended on the limiting nutrient, but with generally increasing C:N:P ratio. The average photosynthetic quotient (PQ) during exponential growth was 1.26 but decreased to <1 under nutrient and light limitation, probably due to photorespiration. The results clearly demonstrate that there are interaction effects between light, temperature and nutrient limitation, and the data suggests greater variability of key parameters at low temperature. Understanding these dynamics will be important for improving models of aquatic primary production and biogeochemical cycles in a warming climate.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0126308</identifier><identifier>PMID: 25993327</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acclimation ; Acclimatization ; Biogeochemical cycles ; Biogeochemistry ; Carbon ; Carbon - deficiency ; Carbon - pharmacology ; Chaetoceros wighamii ; Chlorophyll ; Chlorophyll - agonists ; Chlorophyll - biosynthesis ; Climate change ; Climate models ; Cold ; Cold Temperature ; Cold water ; Cultures ; Diatoms ; Diatoms - drug effects ; Diatoms - growth & development ; Diatoms - metabolism ; Diatoms - radiation effects ; Global warming ; Growth ; Growth rate ; Irradiance ; Light ; Light effects ; Lipids ; Low temperature ; Lowest temperatures ; Metabolism ; Nitrogen - deficiency ; Nitrogen - pharmacology ; Nutrients ; Phosphorus - deficiency ; Phosphorus - pharmacology ; Photons ; Photorespiration ; Photosynthesis ; Photosynthesis - drug effects ; Photosynthesis - radiation effects ; Physiological aspects ; Plankton ; Primary production ; Productivity ; Ratios ; Silicon - deficiency ; Silicon - pharmacology ; Stoichiometry ; Temperature effects ; Variables</subject><ispartof>PloS one, 2015-05, Vol.10 (5), p.e0126308-e0126308</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Spilling et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Spilling et al 2015 Spilling et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-57f5a2e9b4cec4caf8dcde648bfcc84189494ccaf37b608af9f1283642d3b52f3</citedby><cites>FETCH-LOGICAL-c692t-57f5a2e9b4cec4caf8dcde648bfcc84189494ccaf37b608af9f1283642d3b52f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438981/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438981/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25993327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Campbell, Douglas Andrew</contributor><creatorcontrib>Spilling, Kristian</creatorcontrib><creatorcontrib>Ylöstalo, Pasi</creatorcontrib><creatorcontrib>Simis, Stefan</creatorcontrib><creatorcontrib>Seppälä, Jukka</creatorcontrib><title>Interaction Effects of Light, Temperature and Nutrient Limitations (N, P and Si) on Growth, Stoichiometry and Photosynthetic Parameters of the Cold-Water Diatom Chaetoceros wighamii</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Light (20-450 μmol photons m(-2) s(-1)), temperature (3-11 °C) and inorganic nutrient composition (nutrient replete and N, P and Si limitation) were manipulated to study their combined influence on growth, stoichiometry (C:N:P:Chl a) and primary production of the cold water diatom Chaetoceros wighamii. 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Understanding these dynamics will be important for improving models of aquatic primary production and biogeochemical cycles in a warming climate.</description><subject>Acclimation</subject><subject>Acclimatization</subject><subject>Biogeochemical cycles</subject><subject>Biogeochemistry</subject><subject>Carbon</subject><subject>Carbon - deficiency</subject><subject>Carbon - pharmacology</subject><subject>Chaetoceros wighamii</subject><subject>Chlorophyll</subject><subject>Chlorophyll - agonists</subject><subject>Chlorophyll - biosynthesis</subject><subject>Climate change</subject><subject>Climate models</subject><subject>Cold</subject><subject>Cold Temperature</subject><subject>Cold water</subject><subject>Cultures</subject><subject>Diatoms</subject><subject>Diatoms - drug effects</subject><subject>Diatoms - growth & development</subject><subject>Diatoms - metabolism</subject><subject>Diatoms - radiation effects</subject><subject>Global warming</subject><subject>Growth</subject><subject>Growth rate</subject><subject>Irradiance</subject><subject>Light</subject><subject>Light effects</subject><subject>Lipids</subject><subject>Low temperature</subject><subject>Lowest temperatures</subject><subject>Metabolism</subject><subject>Nitrogen - deficiency</subject><subject>Nitrogen - pharmacology</subject><subject>Nutrients</subject><subject>Phosphorus - deficiency</subject><subject>Phosphorus - pharmacology</subject><subject>Photons</subject><subject>Photorespiration</subject><subject>Photosynthesis</subject><subject>Photosynthesis - drug effects</subject><subject>Photosynthesis - radiation effects</subject><subject>Physiological aspects</subject><subject>Plankton</subject><subject>Primary production</subject><subject>Productivity</subject><subject>Ratios</subject><subject>Silicon - deficiency</subject><subject>Silicon - pharmacology</subject><subject>Stoichiometry</subject><subject>Temperature effects</subject><subject>Variables</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk81u1DAQxyMEoqXwBggsIaFW6i6J7ST2BalaSllp1Va0wNFynPHGVRIvtkPpg_F-eD9a7aIeUA6OZn7zH894JkleZ-k4I2X24cYOrpfteGF7GKcZLkjKniT7GSd4VOCUPN3630teeH-TpjlhRfE82cM554Tgcj_5M-0DOKmCsT061RpU8MhqNDPzJhyja-gW0R0GB0j2NTofgjPQh-jvTJDLKI8Oz4_R5cp9ZY5Q1Dlz9jY0x-gqWKMaYzsI7m4FXDY2WH_XhwaCUehSOhmd4FY5oxFNbFuPfshoQp-MDLZDk0ZCsAqc9eg23kp2xrxMnmnZeni1OQ-Sb59PrydfRrOLs-nkZDZSBcdhlJc6lxh4RRUoqqRmtaqhoKzSSjGaMU45VdFOyqpImdRcZ5iRguKaVDnW5CB5u9ZdtNaLTce9yAqGcZbRtIzEdE3UVt6IhTOddHfCSiNWBuvmQrpYaguiAlWqFDNdZIxSXkrQVMdX4wUtcsirqPVxk22oOqhV7LOT7Y7orqc3jZjbX4JSwjjLosDhRsDZnwP4IDrjFbSt7MEO63szFivlEX33D_p4dRtqLmMBptc25lVLUXFCCcY853HuDpLxI1T8auiMiuOpTbTvBBztBEQmwO8wl4P3Ynr19f_Zi--77PsttgHZhsbbdliN6S5I16CKU-Ud6IcmZ6lYbtd9N8Ryu8Rmu2LYm-0Hegi6XyfyF_psIjA</recordid><startdate>20150520</startdate><enddate>20150520</enddate><creator>Spilling, Kristian</creator><creator>Ylöstalo, Pasi</creator><creator>Simis, Stefan</creator><creator>Seppälä, Jukka</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150520</creationdate><title>Interaction Effects of Light, Temperature and Nutrient Limitations (N, P and Si) on Growth, Stoichiometry and Photosynthetic Parameters of the Cold-Water Diatom Chaetoceros wighamii</title><author>Spilling, Kristian ; Ylöstalo, Pasi ; Simis, Stefan ; Seppälä, Jukka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-57f5a2e9b4cec4caf8dcde648bfcc84189494ccaf37b608af9f1283642d3b52f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acclimation</topic><topic>Acclimatization</topic><topic>Biogeochemical cycles</topic><topic>Biogeochemistry</topic><topic>Carbon</topic><topic>Carbon - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spilling, Kristian</au><au>Ylöstalo, Pasi</au><au>Simis, Stefan</au><au>Seppälä, Jukka</au><au>Campbell, Douglas Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction Effects of Light, Temperature and Nutrient Limitations (N, P and Si) on Growth, Stoichiometry and Photosynthetic Parameters of the Cold-Water Diatom Chaetoceros wighamii</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-05-20</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>e0126308</spage><epage>e0126308</epage><pages>e0126308-e0126308</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Light (20-450 μmol photons m(-2) s(-1)), temperature (3-11 °C) and inorganic nutrient composition (nutrient replete and N, P and Si limitation) were manipulated to study their combined influence on growth, stoichiometry (C:N:P:Chl a) and primary production of the cold water diatom Chaetoceros wighamii. During exponential growth, the maximum growth rate (~0.8 d(-1)) was observed at high temperature and light; at 3 °C the growth rate was ~30% lower under similar light conditions. The interaction effect of light and temperature were clearly visible from growth and cellular stoichiometry. The average C:N:P molar ratio was 80:13:1 during exponential growth, but the range, due to different light acclimation, was widest at the lowest temperature, reaching very low C:P (~50) and N:P ratios (~8) at low light and temperature. The C:Chl a ratio had also a wider range at the lowest temperature during exponential growth, ranging 16-48 (weight ratio) at 3 °C compared with 17-33 at 11 °C. During exponential growth, there was no clear trend in the Chl a normalized, initial slope (α*) of the photosynthesis-irradiance (PE) curve, but the maximum photosynthetic production (P(m)) was highest for cultures acclimated to the highest light and temperature. During the stationary growth phase, the stoichiometric relationship depended on the limiting nutrient, but with generally increasing C:N:P ratio. The average photosynthetic quotient (PQ) during exponential growth was 1.26 but decreased to <1 under nutrient and light limitation, probably due to photorespiration. The results clearly demonstrate that there are interaction effects between light, temperature and nutrient limitation, and the data suggests greater variability of key parameters at low temperature. Understanding these dynamics will be important for improving models of aquatic primary production and biogeochemical cycles in a warming climate.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25993327</pmid><doi>10.1371/journal.pone.0126308</doi><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Free; MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Acclimation Acclimatization Biogeochemical cycles Biogeochemistry Carbon Carbon - deficiency Carbon - pharmacology Chaetoceros wighamii Chlorophyll Chlorophyll - agonists Chlorophyll - biosynthesis Climate change Climate models Cold Cold Temperature Cold water Cultures Diatoms Diatoms - drug effects Diatoms - growth & development Diatoms - metabolism Diatoms - radiation effects Global warming Growth Growth rate Irradiance Light Light effects Lipids Low temperature Lowest temperatures Metabolism Nitrogen - deficiency Nitrogen - pharmacology Nutrients Phosphorus - deficiency Phosphorus - pharmacology Photons Photorespiration Photosynthesis Photosynthesis - drug effects Photosynthesis - radiation effects Physiological aspects Plankton Primary production Productivity Ratios Silicon - deficiency Silicon - pharmacology Stoichiometry Temperature effects Variables |
| title | Interaction Effects of Light, Temperature and Nutrient Limitations (N, P and Si) on Growth, Stoichiometry and Photosynthetic Parameters of the Cold-Water Diatom Chaetoceros wighamii |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T18%3A01%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interaction%20Effects%20of%20Light,%20Temperature%20and%20Nutrient%20Limitations%20(N,%20P%20and%20Si)%20on%20Growth,%20Stoichiometry%20and%20Photosynthetic%20Parameters%20of%20the%20Cold-Water%20Diatom%20Chaetoceros%20wighamii&rft.jtitle=PloS%20one&rft.au=Spilling,%20Kristian&rft.date=2015-05-20&rft.volume=10&rft.issue=5&rft.spage=e0126308&rft.epage=e0126308&rft.pages=e0126308-e0126308&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0126308&rft_dat=%3Cgale_plos_%3EA432295926%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1682211407&rft_id=info:pmid/25993327&rft_galeid=A432295926&rft_doaj_id=oai_doaj_org_article_bec7c028f6184497aef4f63096465e5b&rfr_iscdi=true |