A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development
Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for fl...
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| creator | Huang, Jian-Zhi Lin, Chih-Peng Cheng, Ting-Chi Chang, Bill Chia-Han Cheng, Shu-Yu Chen, Yi-Wen Lee, Chen-Yu Chin, Shih-Wen Chen, Fure-Chyi |
| description | Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,838, 758 and 1,147 contigs, respectively, with significant differential expression. PhAGL6a (CUFF.17763), PhAGL6b (CUFF.17763.1), PhMADS1 (CUFF.36625.1), PhMADS4 (CUFF.25909) and PhMADS5 (CUFF.39479.1) were significantly upregulated in the lip-like petal of the peloric mutant. We used real-time PCR analysis of lip-like petals, lip-like sepals and the big lip of peloric mutants to confirm the five genes' expression patterns. PhAGL6a, PhAGL6b and PhMADS4 were strongly expressed in the labellum and significantly upregulated in lip-like petals and lip-like sepals of peloric-mutant flowers. In addition, PhAGL6b was significantly downregulated in the labellum of the big lip mutant, with no change in expression of PhAGL6a. We provide a comprehensive transcript profile and functional analysis of Phalaenopsis floral organs. PhAGL6a PhAGL6b, and PhMADS4 might play crucial roles in the development of the labellum in Phalaenopsis. Our study provides new insights into how the orchid labellum differs and why the petal or sepal converts to a labellum in Phalaenopsis floral mutants. |
| doi_str_mv | 10.1371/journal.pone.0123474 |
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We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,838, 758 and 1,147 contigs, respectively, with significant differential expression. PhAGL6a (CUFF.17763), PhAGL6b (CUFF.17763.1), PhMADS1 (CUFF.36625.1), PhMADS4 (CUFF.25909) and PhMADS5 (CUFF.39479.1) were significantly upregulated in the lip-like petal of the peloric mutant. We used real-time PCR analysis of lip-like petals, lip-like sepals and the big lip of peloric mutants to confirm the five genes' expression patterns. PhAGL6a, PhAGL6b and PhMADS4 were strongly expressed in the labellum and significantly upregulated in lip-like petals and lip-like sepals of peloric-mutant flowers. In addition, PhAGL6b was significantly downregulated in the labellum of the big lip mutant, with no change in expression of PhAGL6a. We provide a comprehensive transcript profile and functional analysis of Phalaenopsis floral organs. PhAGL6a PhAGL6b, and PhMADS4 might play crucial roles in the development of the labellum in Phalaenopsis. Our study provides new insights into how the orchid labellum differs and why the petal or sepal converts to a labellum in Phalaenopsis floral mutants.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0123474</identifier><identifier>PMID: 25970572</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antirrhinum ; Antirrhinum majus ; Arabidopsis ; Arabidopsis thaliana ; Contig Mapping ; Dendrobium ; Flowers ; Flowers & plants ; Flowers - anatomy & histology ; Flowers - genetics ; Flowers - growth & development ; Flowers - metabolism ; Functional analysis ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes ; High-Throughput Nucleotide Sequencing ; Lip ; Molecular Sequence Annotation ; Morphology ; Mutants ; Mutation ; Oncidium ; Orchidaceae ; Orchidaceae - anatomy & histology ; Orchidaceae - classification ; Orchidaceae - genetics ; Orchidaceae - growth & development ; Organ Specificity ; Organs ; Oryza ; Petals ; Phylogeny ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Ribonucleic acid ; RNA ; Science ; Sepals ; Transcription ; Transcription factors ; Transcriptome</subject><ispartof>PloS one, 2015-05, Vol.10 (5), p.e0123474-e0123474</ispartof><rights>2015 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Huang et al 2015 Huang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-eac4e300e540b3ebce4f45ac0260c0ea43aa6f6460bd94ccb46b6994f3bf04403</citedby><cites>FETCH-LOGICAL-c526t-eac4e300e540b3ebce4f45ac0260c0ea43aa6f6460bd94ccb46b6994f3bf04403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430480/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430480/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25970572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Bendahmane, Mohammed</contributor><creatorcontrib>Huang, Jian-Zhi</creatorcontrib><creatorcontrib>Lin, Chih-Peng</creatorcontrib><creatorcontrib>Cheng, Ting-Chi</creatorcontrib><creatorcontrib>Chang, Bill Chia-Han</creatorcontrib><creatorcontrib>Cheng, Shu-Yu</creatorcontrib><creatorcontrib>Chen, Yi-Wen</creatorcontrib><creatorcontrib>Lee, Chen-Yu</creatorcontrib><creatorcontrib>Chin, Shih-Wen</creatorcontrib><creatorcontrib>Chen, Fure-Chyi</creatorcontrib><title>A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,838, 758 and 1,147 contigs, respectively, with significant differential expression. PhAGL6a (CUFF.17763), PhAGL6b (CUFF.17763.1), PhMADS1 (CUFF.36625.1), PhMADS4 (CUFF.25909) and PhMADS5 (CUFF.39479.1) were significantly upregulated in the lip-like petal of the peloric mutant. We used real-time PCR analysis of lip-like petals, lip-like sepals and the big lip of peloric mutants to confirm the five genes' expression patterns. PhAGL6a, PhAGL6b and PhMADS4 were strongly expressed in the labellum and significantly upregulated in lip-like petals and lip-like sepals of peloric-mutant flowers. In addition, PhAGL6b was significantly downregulated in the labellum of the big lip mutant, with no change in expression of PhAGL6a. We provide a comprehensive transcript profile and functional analysis of Phalaenopsis floral organs. PhAGL6a PhAGL6b, and PhMADS4 might play crucial roles in the development of the labellum in Phalaenopsis. Our study provides new insights into how the orchid labellum differs and why the petal or sepal converts to a labellum in Phalaenopsis floral mutants.</description><subject>Antirrhinum</subject><subject>Antirrhinum majus</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Contig Mapping</subject><subject>Dendrobium</subject><subject>Flowers</subject><subject>Flowers & plants</subject><subject>Flowers - anatomy & histology</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Lip</subject><subject>Molecular Sequence Annotation</subject><subject>Morphology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Oncidium</subject><subject>Orchidaceae</subject><subject>Orchidaceae - anatomy & histology</subject><subject>Orchidaceae - classification</subject><subject>Orchidaceae - genetics</subject><subject>Orchidaceae - growth & development</subject><subject>Organ Specificity</subject><subject>Organs</subject><subject>Oryza</subject><subject>Petals</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Science</subject><subject>Sepals</subject><subject>Transcription</subject><subject>Transcription factors</subject><subject>Transcriptome</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1rFDEUDaLYuvoPRAO--LJrPu5kNi9CKVULBX1Q8C1kMne6s2SSMZnd4r9vtjstrUgeEpJzTs65HELecrbisuaftnGXgvWrMQZcMS4k1PCMnHItxVIJJp8_Op-QVzlvGavkWqmX5ERUumZVLU7J7zPaIg1xH2nnY7KeTsmG7FI_TnFAmnCP1mfq_A4z7cMU6Y-N9RZDHHOfaUxu07cH7g2mIrVHH8cBw_SavOgKEd_M-4L8-nLx8_zb8ur718vzs6ulq4SalmgdoGQMK2CNxMYhdFBZx4RijqEFaa3qFCjWtBqca0A1SmvoZNMxACYX5P1Rd_Qxm3ko2XC1ZnVZWhbE5RHRRrs1Y-oHm_6aaHtzdxHTtbFp6p1H02rRaOR8LWoFoJWWSgrRVKqDtuZFbEE-z7_tmgFbV4KWmT0RffoS-o25jnsDIBmsD3Y_zgIp_ikjnczQZ4fe24Bxd-ebCyW5hAL98A_0_-ngiHIp5pywezDDmTkU5Z5lDkUxc1EK7d3jIA-k-2bIWzMrvIQ</recordid><startdate>20150513</startdate><enddate>20150513</enddate><creator>Huang, Jian-Zhi</creator><creator>Lin, Chih-Peng</creator><creator>Cheng, Ting-Chi</creator><creator>Chang, Bill Chia-Han</creator><creator>Cheng, Shu-Yu</creator><creator>Chen, Yi-Wen</creator><creator>Lee, Chen-Yu</creator><creator>Chin, Shih-Wen</creator><creator>Chen, Fure-Chyi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150513</creationdate><title>A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development</title><author>Huang, Jian-Zhi ; Lin, Chih-Peng ; Cheng, Ting-Chi ; Chang, Bill Chia-Han ; Cheng, Shu-Yu ; Chen, Yi-Wen ; Lee, Chen-Yu ; Chin, Shih-Wen ; Chen, Fure-Chyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-eac4e300e540b3ebce4f45ac0260c0ea43aa6f6460bd94ccb46b6994f3bf04403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antirrhinum</topic><topic>Antirrhinum majus</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Contig Mapping</topic><topic>Dendrobium</topic><topic>Flowers</topic><topic>Flowers & plants</topic><topic>Flowers - anatomy & histology</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Flowers - metabolism</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Lip</topic><topic>Molecular Sequence Annotation</topic><topic>Morphology</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Oncidium</topic><topic>Orchidaceae</topic><topic>Orchidaceae - anatomy & histology</topic><topic>Orchidaceae - classification</topic><topic>Orchidaceae - genetics</topic><topic>Orchidaceae - growth & development</topic><topic>Organ Specificity</topic><topic>Organs</topic><topic>Oryza</topic><topic>Petals</topic><topic>Phylogeny</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Science</topic><topic>Sepals</topic><topic>Transcription</topic><topic>Transcription factors</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Jian-Zhi</creatorcontrib><creatorcontrib>Lin, Chih-Peng</creatorcontrib><creatorcontrib>Cheng, Ting-Chi</creatorcontrib><creatorcontrib>Chang, Bill Chia-Han</creatorcontrib><creatorcontrib>Cheng, Shu-Yu</creatorcontrib><creatorcontrib>Chen, Yi-Wen</creatorcontrib><creatorcontrib>Lee, Chen-Yu</creatorcontrib><creatorcontrib>Chin, Shih-Wen</creatorcontrib><creatorcontrib>Chen, Fure-Chyi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Jian-Zhi</au><au>Lin, Chih-Peng</au><au>Cheng, Ting-Chi</au><au>Chang, Bill Chia-Han</au><au>Cheng, Shu-Yu</au><au>Chen, Yi-Wen</au><au>Lee, Chen-Yu</au><au>Chin, Shih-Wen</au><au>Chen, Fure-Chyi</au><au>Bendahmane, Mohammed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-05-13</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>e0123474</spage><epage>e0123474</epage><pages>e0123474-e0123474</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Phalaenopsis has a zygomorphic floral structure, including three outer tepals, two lateral inner tepals and a highly modified inner median tepal called labellum or lip; however, the regulation of its organ development remains unelucidated. We generated RNA-seq reads with the Illumina platform for floral organs of the Phalaenopsis wild-type and peloric mutant with a lip-like petal. A total of 43,552 contigs were obtained after de novo assembly. We used differentially expressed gene profiling to compare the transcriptional changes in floral organs for both the wild-type and peloric mutant. Pair-wise comparison of sepals, petals and labellum between peloric mutant and its wild-type revealed 1,838, 758 and 1,147 contigs, respectively, with significant differential expression. PhAGL6a (CUFF.17763), PhAGL6b (CUFF.17763.1), PhMADS1 (CUFF.36625.1), PhMADS4 (CUFF.25909) and PhMADS5 (CUFF.39479.1) were significantly upregulated in the lip-like petal of the peloric mutant. We used real-time PCR analysis of lip-like petals, lip-like sepals and the big lip of peloric mutants to confirm the five genes' expression patterns. PhAGL6a, PhAGL6b and PhMADS4 were strongly expressed in the labellum and significantly upregulated in lip-like petals and lip-like sepals of peloric-mutant flowers. In addition, PhAGL6b was significantly downregulated in the labellum of the big lip mutant, with no change in expression of PhAGL6a. We provide a comprehensive transcript profile and functional analysis of Phalaenopsis floral organs. PhAGL6a PhAGL6b, and PhMADS4 might play crucial roles in the development of the labellum in Phalaenopsis. Our study provides new insights into how the orchid labellum differs and why the petal or sepal converts to a labellum in Phalaenopsis floral mutants.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25970572</pmid><doi>10.1371/journal.pone.0123474</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1680707093 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Antirrhinum Antirrhinum majus Arabidopsis Arabidopsis thaliana Contig Mapping Dendrobium Flowers Flowers & plants Flowers - anatomy & histology Flowers - genetics Flowers - growth & development Flowers - metabolism Functional analysis Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes High-Throughput Nucleotide Sequencing Lip Molecular Sequence Annotation Morphology Mutants Mutation Oncidium Orchidaceae Orchidaceae - anatomy & histology Orchidaceae - classification Orchidaceae - genetics Orchidaceae - growth & development Organ Specificity Organs Oryza Petals Phylogeny Plant Proteins - genetics Plant Proteins - metabolism Ribonucleic acid RNA Science Sepals Transcription Transcription factors Transcriptome |
| title | A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T16%3A16%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20de%20novo%20floral%20transcriptome%20reveals%20clues%20into%20Phalaenopsis%20orchid%20flower%20development&rft.jtitle=PloS%20one&rft.au=Huang,%20Jian-Zhi&rft.date=2015-05-13&rft.volume=10&rft.issue=5&rft.spage=e0123474&rft.epage=e0123474&rft.pages=e0123474-e0123474&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0123474&rft_dat=%3Cproquest_plos_%3E1681263134%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1680707093&rft_id=info:pmid/25970572&rft_doaj_id=oai_doaj_org_article_d92b9e1182764496936322b56f4d7109&rfr_iscdi=true |