C-Peptide-based assessment of insulin secretion in the Zucker Fatty rat: a modelistic study
A C-peptide-based assessment of β-cell function was performed here in the Zucker fatty rat, a suitable animal model of human metabolic syndrome. To this aim, a 90-min intravenous glucose tolerance test (IVGTT) was performed in seven Zucker fatty rats (ZFR), 7-to-9 week-old, and seven age-matched Zuc...
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| description | A C-peptide-based assessment of β-cell function was performed here in the Zucker fatty rat, a suitable animal model of human metabolic syndrome. To this aim, a 90-min intravenous glucose tolerance test (IVGTT) was performed in seven Zucker fatty rats (ZFR), 7-to-9 week-old, and seven age-matched Zucker lean rats (ZLR). The minimal model of C-peptide (CPMM), originally introduced for humans, was adapted to Zucker rats and then applied to interpret IVGTT data. For a comprehensive evaluation of glucose tolerance in ZFR, CPMM was applied in combination with the minimal model of glucose kinetics (GKMM). Our results showed that the present CPMM-based interpretation of data is able to: 1) provide a suitable fit of C-Peptide data; 2) achieve a satisfactory estimation of parameters of interest 3) quantify both insulin secretion by estimating the time course of pre-hepatic secretion rate, SR(t), and total insulin secretion, TIS, and pancreatic sensitivity by means of three specific indexes of β-cell responsiveness to glucose stimulus (first-phase, Ф(1), second-phase, Ф(2), and steady-state, Ф(ss), never assessed in Zucker rats before; 4) detect the significant enhancement of insulin secretion in the ZFR, in face of a severe insulin-resistant state, previously observed only using a purely experimental approach. Thus, the methodology presented here represents a reliable tool to assess β-cell function in the Zucker rat, and opens new possibilities for the quantification of further processes involved in glucose homeostasis such as the hepatic insulin degradation. |
| doi_str_mv | 10.1371/journal.pone.0125252 |
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To this aim, a 90-min intravenous glucose tolerance test (IVGTT) was performed in seven Zucker fatty rats (ZFR), 7-to-9 week-old, and seven age-matched Zucker lean rats (ZLR). The minimal model of C-peptide (CPMM), originally introduced for humans, was adapted to Zucker rats and then applied to interpret IVGTT data. For a comprehensive evaluation of glucose tolerance in ZFR, CPMM was applied in combination with the minimal model of glucose kinetics (GKMM). Our results showed that the present CPMM-based interpretation of data is able to: 1) provide a suitable fit of C-Peptide data; 2) achieve a satisfactory estimation of parameters of interest 3) quantify both insulin secretion by estimating the time course of pre-hepatic secretion rate, SR(t), and total insulin secretion, TIS, and pancreatic sensitivity by means of three specific indexes of β-cell responsiveness to glucose stimulus (first-phase, Ф(1), second-phase, Ф(2), and steady-state, Ф(ss), never assessed in Zucker rats before; 4) detect the significant enhancement of insulin secretion in the ZFR, in face of a severe insulin-resistant state, previously observed only using a purely experimental approach. Thus, the methodology presented here represents a reliable tool to assess β-cell function in the Zucker rat, and opens new possibilities for the quantification of further processes involved in glucose homeostasis such as the hepatic insulin degradation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0125252</identifier><identifier>PMID: 25938808</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Blood Glucose ; C-Peptide - metabolism ; Care and treatment ; Data interpretation ; Diagnosis ; Disease Models, Animal ; Glucose ; Glucose - metabolism ; Glucose tolerance ; Glucose Tolerance Test ; Homeostasis ; Insulin ; Insulin - blood ; Insulin - metabolism ; Insulin resistance ; Insulin Secretion ; Insulin-Secreting Cells - metabolism ; Intravenous administration ; Kinetics ; Laboratories ; Male ; Metabolic disorders ; Metabolic syndrome ; Metabolic syndrome X ; Pancreas ; Parameter estimation ; Peptides ; Rats ; Rats, Zucker ; Risk factors ; Rodents ; Secretion ; Time Factors</subject><ispartof>PloS one, 2015-05, Vol.10 (5), p.e0125252-e0125252</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Di Nardo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Di Nardo et al 2015 Di Nardo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c1f7c2175f71c936270b5d562a6125fc61aedc4b4973ca79a0ea105e9fdb72203</citedby><cites>FETCH-LOGICAL-c692t-c1f7c2175f71c936270b5d562a6125fc61aedc4b4973ca79a0ea105e9fdb72203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418729/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418729/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25938808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Di Nardo, Francesco</creatorcontrib><creatorcontrib>Cogo, Carla E</creatorcontrib><creatorcontrib>Faelli, Emanuela</creatorcontrib><creatorcontrib>Morettini, Micaela</creatorcontrib><creatorcontrib>Burattini, Laura</creatorcontrib><creatorcontrib>Ruggeri, Piero</creatorcontrib><title>C-Peptide-based assessment of insulin secretion in the Zucker Fatty rat: a modelistic study</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A C-peptide-based assessment of β-cell function was performed here in the Zucker fatty rat, a suitable animal model of human metabolic syndrome. To this aim, a 90-min intravenous glucose tolerance test (IVGTT) was performed in seven Zucker fatty rats (ZFR), 7-to-9 week-old, and seven age-matched Zucker lean rats (ZLR). The minimal model of C-peptide (CPMM), originally introduced for humans, was adapted to Zucker rats and then applied to interpret IVGTT data. For a comprehensive evaluation of glucose tolerance in ZFR, CPMM was applied in combination with the minimal model of glucose kinetics (GKMM). Our results showed that the present CPMM-based interpretation of data is able to: 1) provide a suitable fit of C-Peptide data; 2) achieve a satisfactory estimation of parameters of interest 3) quantify both insulin secretion by estimating the time course of pre-hepatic secretion rate, SR(t), and total insulin secretion, TIS, and pancreatic sensitivity by means of three specific indexes of β-cell responsiveness to glucose stimulus (first-phase, Ф(1), second-phase, Ф(2), and steady-state, Ф(ss), never assessed in Zucker rats before; 4) detect the significant enhancement of insulin secretion in the ZFR, in face of a severe insulin-resistant state, previously observed only using a purely experimental approach. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Di Nardo, Francesco</au><au>Cogo, Carla E</au><au>Faelli, Emanuela</au><au>Morettini, Micaela</au><au>Burattini, Laura</au><au>Ruggeri, Piero</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-Peptide-based assessment of insulin secretion in the Zucker Fatty rat: a modelistic study</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-05-04</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>e0125252</spage><epage>e0125252</epage><pages>e0125252-e0125252</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A C-peptide-based assessment of β-cell function was performed here in the Zucker fatty rat, a suitable animal model of human metabolic syndrome. To this aim, a 90-min intravenous glucose tolerance test (IVGTT) was performed in seven Zucker fatty rats (ZFR), 7-to-9 week-old, and seven age-matched Zucker lean rats (ZLR). The minimal model of C-peptide (CPMM), originally introduced for humans, was adapted to Zucker rats and then applied to interpret IVGTT data. For a comprehensive evaluation of glucose tolerance in ZFR, CPMM was applied in combination with the minimal model of glucose kinetics (GKMM). Our results showed that the present CPMM-based interpretation of data is able to: 1) provide a suitable fit of C-Peptide data; 2) achieve a satisfactory estimation of parameters of interest 3) quantify both insulin secretion by estimating the time course of pre-hepatic secretion rate, SR(t), and total insulin secretion, TIS, and pancreatic sensitivity by means of three specific indexes of β-cell responsiveness to glucose stimulus (first-phase, Ф(1), second-phase, Ф(2), and steady-state, Ф(ss), never assessed in Zucker rats before; 4) detect the significant enhancement of insulin secretion in the ZFR, in face of a severe insulin-resistant state, previously observed only using a purely experimental approach. Thus, the methodology presented here represents a reliable tool to assess β-cell function in the Zucker rat, and opens new possibilities for the quantification of further processes involved in glucose homeostasis such as the hepatic insulin degradation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25938808</pmid><doi>10.1371/journal.pone.0125252</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blood Glucose C-Peptide - metabolism Care and treatment Data interpretation Diagnosis Disease Models, Animal Glucose Glucose - metabolism Glucose tolerance Glucose Tolerance Test Homeostasis Insulin Insulin - blood Insulin - metabolism Insulin resistance Insulin Secretion Insulin-Secreting Cells - metabolism Intravenous administration Kinetics Laboratories Male Metabolic disorders Metabolic syndrome Metabolic syndrome X Pancreas Parameter estimation Peptides Rats Rats, Zucker Risk factors Rodents Secretion Time Factors |
| title | C-Peptide-based assessment of insulin secretion in the Zucker Fatty rat: a modelistic study |
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