A novel approach to identifying physical markers of cryo-damage in bull spermatozoa
Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps...
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| creator | Yoon, Sung-Jae Kwon, Woo-Sung Rahman, Md Saidur Lee, June-Sub Pang, Myung-Geol |
| description | Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa collected from 9 individual bull testes. The motility (%), motion kinematics, capacitation status, mitochondrial activity, and viability of bovine spermatozoa at each step of the cryopreservation process were assessed using computer-assisted sperm analysis, Hoechst 33258/chlortetracycline fluorescence, rhodamine 123 staining, and hypo-osmotic swelling test, respectively. The results demonstrate that the cryopreservation steps reduced motility (%), rapid speed (%), and mitochondrial activity, whereas medium/slow speed (%), and the acrosome reaction were increased (P < 0.05). Differences (Δ) of the acrosome reaction were higher in dilution/cooling step (P < 0.05), whereas differences (Δ) of motility, rapid speed, and non-progressive motility were higher in cryoprotectant and freezing as compared to dilution/cooling (P < 0.05). On the other hand, differences (Δ) of mitochondrial activity, viability, and progressive motility were higher in freezing step (P < 0.05) while the difference (Δ) of the acrosome reaction was higher in dilution/cooling (P < 0.05). Based on these results, we propose that freezing / thawing steps are the most critical in cryopreservation and may provide a logical ground of understanding on the cryo-damage. Moreover, these sperm parameters might be used as physical markers of sperm cryo-damage. |
| doi_str_mv | 10.1371/journal.pone.0126232 |
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During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa collected from 9 individual bull testes. The motility (%), motion kinematics, capacitation status, mitochondrial activity, and viability of bovine spermatozoa at each step of the cryopreservation process were assessed using computer-assisted sperm analysis, Hoechst 33258/chlortetracycline fluorescence, rhodamine 123 staining, and hypo-osmotic swelling test, respectively. The results demonstrate that the cryopreservation steps reduced motility (%), rapid speed (%), and mitochondrial activity, whereas medium/slow speed (%), and the acrosome reaction were increased (P < 0.05). Differences (Δ) of the acrosome reaction were higher in dilution/cooling step (P < 0.05), whereas differences (Δ) of motility, rapid speed, and non-progressive motility were higher in cryoprotectant and freezing as compared to dilution/cooling (P < 0.05). On the other hand, differences (Δ) of mitochondrial activity, viability, and progressive motility were higher in freezing step (P < 0.05) while the difference (Δ) of the acrosome reaction was higher in dilution/cooling (P < 0.05). Based on these results, we propose that freezing / thawing steps are the most critical in cryopreservation and may provide a logical ground of understanding on the cryo-damage. Moreover, these sperm parameters might be used as physical markers of sperm cryo-damage.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0126232</identifier><identifier>PMID: 25938413</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acrosome Reaction ; Animal sciences ; Animals ; Biomarkers ; Capacitation ; Cattle ; Chlortetracycline ; Cooling ; Cooling rate ; Cryopreservation ; Cryopreservation - methods ; Cryoprotectors ; Damage detection ; Dilution ; Fatty acids ; Fertility ; Fish ; Flow Cytometry ; Fluorescence ; Freezing ; Infertility ; Kinematics ; Livestock ; Livestock industry ; Male ; Markers ; Mitochondria ; Mitochondria - metabolism ; Motility ; Nitrogen ; Oncorhynchus mykiss ; Physiological aspects ; Rhodamine ; Semen Analysis ; Semen Preservation - methods ; Sperm ; Sperm Motility ; Spermatozoa ; Spermatozoa - cytology ; Spermatozoa - physiology ; Testes ; Thawing ; Viability ; Zoology</subject><ispartof>PloS one, 2015-05, Vol.10 (5), p.e0126232-e0126232</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Yoon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Yoon et al 2015 Yoon et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-93274ff967c7882b95f9d6b1e1dd4b3d74a21770c99e77f9a87a33df1c0c91f93</citedby><cites>FETCH-LOGICAL-c692t-93274ff967c7882b95f9d6b1e1dd4b3d74a21770c99e77f9a87a33df1c0c91f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418755/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4418755/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25938413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yan, Wei</contributor><creatorcontrib>Yoon, Sung-Jae</creatorcontrib><creatorcontrib>Kwon, Woo-Sung</creatorcontrib><creatorcontrib>Rahman, Md Saidur</creatorcontrib><creatorcontrib>Lee, June-Sub</creatorcontrib><creatorcontrib>Pang, Myung-Geol</creatorcontrib><title>A novel approach to identifying physical markers of cryo-damage in bull spermatozoa</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. 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Differences (Δ) of the acrosome reaction were higher in dilution/cooling step (P < 0.05), whereas differences (Δ) of motility, rapid speed, and non-progressive motility were higher in cryoprotectant and freezing as compared to dilution/cooling (P < 0.05). On the other hand, differences (Δ) of mitochondrial activity, viability, and progressive motility were higher in freezing step (P < 0.05) while the difference (Δ) of the acrosome reaction was higher in dilution/cooling (P < 0.05). Based on these results, we propose that freezing / thawing steps are the most critical in cryopreservation and may provide a logical ground of understanding on the cryo-damage. Moreover, these sperm parameters might be used as physical markers of sperm cryo-damage.</description><subject>Acrosome Reaction</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Capacitation</subject><subject>Cattle</subject><subject>Chlortetracycline</subject><subject>Cooling</subject><subject>Cooling rate</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotectors</subject><subject>Damage detection</subject><subject>Dilution</subject><subject>Fatty acids</subject><subject>Fertility</subject><subject>Fish</subject><subject>Flow Cytometry</subject><subject>Fluorescence</subject><subject>Freezing</subject><subject>Infertility</subject><subject>Kinematics</subject><subject>Livestock</subject><subject>Livestock industry</subject><subject>Male</subject><subject>Markers</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Motility</subject><subject>Nitrogen</subject><subject>Oncorhynchus mykiss</subject><subject>Physiological aspects</subject><subject>Rhodamine</subject><subject>Semen Analysis</subject><subject>Semen Preservation - methods</subject><subject>Sperm</subject><subject>Sperm Motility</subject><subject>Spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><subject>Testes</subject><subject>Thawing</subject><subject>Viability</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLgujFjPlok-ZGGBY_BhYWXPU2pPnoZEybbtIujr_ezE53mcpeSC9aTp_3PTknb5a9hGAJMYUftn4MnXDL3nd6CSAiCKNH2SlkGC0IAvjx0fdJ9izGLQAlrgh5mp2gkuGqgPg0u1rlnb_RLhd9H7yQm3zwuVW6G6zZ2a7J-80uWilc3orwS4eYe5PLsPMLJVrR6Nx2eT06l8deh1YM_o8Xz7MnRrioX0zvs-zH50_fz78uLi6_rM9XFwtJGBoW6XC0MIYRKmlVoZqVhilSQw2VKmqsaCEQpBRIxjSlhomKCoyVgTKVoGH4LHt98O2dj3zaR-SQ0KqkhDKSiPWBUF5seR9sGmLHvbD8tuBDw0UYrHSaG4RTE6lkhWWR1HVRaoUMVjUgJZEoeX2cuo11q5VMKwrCzUznfzq74Y2_4UUBK1qWyeDdZBD89ajjwFsbpXZOdNqP-3NXAFYIQJDQN_-gD083UY1IA9jO-NRX7k35qsAg3T659Vo-QKVH6dbKFB5jU30meD8TJGbQv4dGjDHy9dW3_2cvf87Zt0fsRgs3bKJ342B9F-dgcQBl8DEGbe6XDAHfZ_9uG3yffT5lP8leHV_Qvegu7PgvdDT-NQ</recordid><startdate>20150504</startdate><enddate>20150504</enddate><creator>Yoon, Sung-Jae</creator><creator>Kwon, Woo-Sung</creator><creator>Rahman, Md Saidur</creator><creator>Lee, June-Sub</creator><creator>Pang, Myung-Geol</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150504</creationdate><title>A novel approach to identifying physical markers of cryo-damage in bull spermatozoa</title><author>Yoon, Sung-Jae ; Kwon, Woo-Sung ; Rahman, Md Saidur ; Lee, June-Sub ; Pang, Myung-Geol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-93274ff967c7882b95f9d6b1e1dd4b3d74a21770c99e77f9a87a33df1c0c91f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acrosome Reaction</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Biomarkers</topic><topic>Capacitation</topic><topic>Cattle</topic><topic>Chlortetracycline</topic><topic>Cooling</topic><topic>Cooling rate</topic><topic>Cryopreservation</topic><topic>Cryopreservation - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoon, Sung-Jae</au><au>Kwon, Woo-Sung</au><au>Rahman, Md Saidur</au><au>Lee, June-Sub</au><au>Pang, Myung-Geol</au><au>Yan, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel approach to identifying physical markers of cryo-damage in bull spermatozoa</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-05-04</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>e0126232</spage><epage>e0126232</epage><pages>e0126232-e0126232</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. 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Differences (Δ) of the acrosome reaction were higher in dilution/cooling step (P < 0.05), whereas differences (Δ) of motility, rapid speed, and non-progressive motility were higher in cryoprotectant and freezing as compared to dilution/cooling (P < 0.05). On the other hand, differences (Δ) of mitochondrial activity, viability, and progressive motility were higher in freezing step (P < 0.05) while the difference (Δ) of the acrosome reaction was higher in dilution/cooling (P < 0.05). Based on these results, we propose that freezing / thawing steps are the most critical in cryopreservation and may provide a logical ground of understanding on the cryo-damage. Moreover, these sperm parameters might be used as physical markers of sperm cryo-damage.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25938413</pmid><doi>10.1371/journal.pone.0126232</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acrosome Reaction Animal sciences Animals Biomarkers Capacitation Cattle Chlortetracycline Cooling Cooling rate Cryopreservation Cryopreservation - methods Cryoprotectors Damage detection Dilution Fatty acids Fertility Fish Flow Cytometry Fluorescence Freezing Infertility Kinematics Livestock Livestock industry Male Markers Mitochondria Mitochondria - metabolism Motility Nitrogen Oncorhynchus mykiss Physiological aspects Rhodamine Semen Analysis Semen Preservation - methods Sperm Sperm Motility Spermatozoa Spermatozoa - cytology Spermatozoa - physiology Testes Thawing Viability Zoology |
| title | A novel approach to identifying physical markers of cryo-damage in bull spermatozoa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T04%3A01%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20approach%20to%20identifying%20physical%20markers%20of%20cryo-damage%20in%20bull%20spermatozoa&rft.jtitle=PloS%20one&rft.au=Yoon,%20Sung-Jae&rft.date=2015-05-04&rft.volume=10&rft.issue=5&rft.spage=e0126232&rft.epage=e0126232&rft.pages=e0126232-e0126232&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0126232&rft_dat=%3Cgale_plos_%3EA430193610%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1678576796&rft_id=info:pmid/25938413&rft_galeid=A430193610&rft_doaj_id=oai_doaj_org_article_f2377fcdc83c4767b45ed2f3db0656c2&rfr_iscdi=true |