Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses
As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilizati...
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| Veröffentlicht in: | PloS one 2015-04, Vol.10 (4), p.e0118963-e0118963 |
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| creator | Pastorino, Boris Baronti, Cecile Gould, Ernest A Charrel, Remi N de Lamballerie, Xavier |
| description | As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection. |
| doi_str_mv | 10.1371/journal.pone.0118963 |
| format | Article |
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The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0118963</identifier><identifier>PMID: 25923434</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Cell Line ; Chikungunya virus ; Collection ; Consortia ; Deactivation ; Deoxyribonucleic acid ; DNA ; DNA viruses ; Excipients - pharmacology ; Formulations ; Freeze drying ; Freeze Drying - methods ; Humans ; Inactivation ; Infectious diseases ; Infectivity ; Mimicry ; Public health ; Ribonucleic acid ; RNA ; RNA viruses ; RNA Viruses - drug effects ; RNA Viruses - pathogenicity ; Shipping ; Sorbitol ; Stabilizers (agents) ; Sucrose ; Sugar ; Temperature ; Thermal stability ; Tissue culture ; Trehalose ; Viruses</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0118963-e0118963</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Pastorino et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Pastorino et al 2015 Pastorino et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-78f50042c5a8f309b78e4644570b8cc791aacfec2edeed0572c83bd600d4d9233</citedby><cites>FETCH-LOGICAL-c758t-78f50042c5a8f309b78e4644570b8cc791aacfec2edeed0572c83bd600d4d9233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414529/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414529/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25923434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pastorino, Boris</creatorcontrib><creatorcontrib>Baronti, Cecile</creatorcontrib><creatorcontrib>Gould, Ernest A</creatorcontrib><creatorcontrib>Charrel, Remi N</creatorcontrib><creatorcontrib>de Lamballerie, Xavier</creatorcontrib><title>Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>As a partner of the European Virus Archive (EVA) FP7 project, our laboratory maintains a large collection of freeze-dried viruses. The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.</description><subject>Cell Line</subject><subject>Chikungunya virus</subject><subject>Collection</subject><subject>Consortia</subject><subject>Deactivation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA viruses</subject><subject>Excipients - pharmacology</subject><subject>Formulations</subject><subject>Freeze drying</subject><subject>Freeze Drying - methods</subject><subject>Humans</subject><subject>Inactivation</subject><subject>Infectious diseases</subject><subject>Infectivity</subject><subject>Mimicry</subject><subject>Public health</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA Viruses - drug effects</subject><subject>RNA Viruses - 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The distribution of these viruses to academic researchers, public health organizations and industry is one major aim of the EVA consortium. It is known that lyophilization requires appropriate stabilizers to prevent inactivation of the virus. However, few studies have investigated the influence of different stabilizers and lyophilization protocols on the thermostability of different viruses. In order to identify optimal lyophilization conditions that will deliver maximum retention of viral infectivity titre, different stabilizer formulations containing trehalose, sorbitol, sucrose or foetal bovine serum were evaluated for their efficacy in stabilizing a representative panel of freeze dried viruses at different storage temperatures (-20°C, +4°C and +20°C) for one week, the two latter mimicking suboptimal shipping conditions. The Tissue Culture Infectious Dose 50% (TCID50) assay was used to compare the titres of infectious virus. The results obtained using four relevant and model viruses (enveloped/non enveloped RNA/DNA viruses) still serve to improve the freeze drying conditions needed for the development and the distribution of a large virus collection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25923434</pmid><doi>10.1371/journal.pone.0118963</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Cell Line Chikungunya virus Collection Consortia Deactivation Deoxyribonucleic acid DNA DNA viruses Excipients - pharmacology Formulations Freeze drying Freeze Drying - methods Humans Inactivation Infectious diseases Infectivity Mimicry Public health Ribonucleic acid RNA RNA viruses RNA Viruses - drug effects RNA Viruses - pathogenicity Shipping Sorbitol Stabilizers (agents) Sucrose Sugar Temperature Thermal stability Tissue culture Trehalose Viruses |
| title | Effect of chemical stabilizers on the thermostability and infectivity of a representative panel of freeze dried viruses |
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