High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets
Obesity has been associated with elevated plasma anandamide levels. In addition, anandamide has been shown to stimulate insulin secretion in vitro, suggesting that anandamide might be linked to hyperinsulinemia. To determine whether high-fat diet-induced insulin resistance increases anandamide level...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-04, Vol.10 (4), p.e0123558-e0123558 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0123558 |
|---|---|
| container_issue | 4 |
| container_start_page | e0123558 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Woolcott, Orison O Richey, Joyce M Kabir, Morvarid Chow, Robert H Iyer, Malini S Kirkman, Erlinda L Stefanovski, Darko Lottati, Maya Kim, Stella P Harrison, L Nicole Ionut, Viorica Zheng, Dan Hsu, Isabel R Catalano, Karyn J Chiu, Jenny D Bradshaw, Heather Wu, Qiang Kolka, Cathryn M Bergman, Richard N |
| description | Obesity has been associated with elevated plasma anandamide levels. In addition, anandamide has been shown to stimulate insulin secretion in vitro, suggesting that anandamide might be linked to hyperinsulinemia.
To determine whether high-fat diet-induced insulin resistance increases anandamide levels and potentiates the insulinotropic effect of anandamide in isolated pancreatic islets.
Dogs were fed a high-fat diet (n = 9) for 22 weeks. Abdominal fat depot was quantified by MRI. Insulin sensitivity was assessed by the euglycemic-hyperinsulinemic clamp. Fasting plasma endocannabinoid levels were analyzed by liquid chromatography-mass spectrometry. All metabolic assessments were performed before and after fat diet regimen. At the end of the study, pancreatic islets were isolated prior to euthanasia to test the in vitro effect of anandamide on islet hormones. mRNA expression of cannabinoid receptors was determined in intact islets. The findings in vitro were compared with those from animals fed a control diet (n = 7).
Prolonged fat feeding increased abdominal fat content by 81.3±21.6% (mean±S.E.M, P |
| doi_str_mv | 10.1371/journal.pone.0123558 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1672094832</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A429328237</galeid><doaj_id>oai_doaj_org_article_a9550449b61140ec87d3e78492fae4f0</doaj_id><sourcerecordid>A429328237</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-6553208da24b1f3f46637f864621f6ecb44160fdd6b2500a5d6d630c303ccb6d3</originalsourceid><addsrcrecordid>eNqNk1trFDEUxwdRbK1-A9EBQfRh19xn5kUoRW2hUPD2GrLJyW5KNlmTTNHv4oc1607LrvRB8pCQ8zv_5Nya5jlGc0w7_O46jikoP9_EAHOECeW8f9Ac44GSmSCIPtw7HzVPcr5GiNNeiMfNEeE950PHjpvf5265mllVWuOgzFwwowbTupBH70KbILtcVNDQmgi5DbFUm06gMrQbr_JatSqoYNTaGWg93IDPbUztJhYIxakC-_ZJNpYUN063YC3orWDrcvSVNa1WwYUKZg8lP20eWeUzPJv2k-bbxw9fz85nl1efLs5OL2daDKTMBOeUoN4owhbYUsuEoJ3tBRMEWwF6wRgWyBojFoQjpLgRRlCkKaJaL4ShJ83Lne7GxyynxGaJRUfQwHpKKnGxI0xU13KT3FqlXzIqJ_9exLSUKhWnPUg1cI4YGxYCY4ZA952h0PVsIFYBs6hqvZ9eGxdrMLrmKSl_IHpoCW4ll_FGMjrggfAq8GYSSPHHCLnItcsavFcB4rj7t8C0fruir_5B749uopaqBuCCrQVSeisqTxmpTdQT2lVqfg9Vl4G107ULrav3Bw5vDxwqU-BnWaoxZ3nx5fP_s1ffD9nXe-wKlC-r2j9jcTHkQ5DtQJ1izgnsXZIxktshus2G3A6RnIaour3YL9Cd0-3U0D9Z5hmp</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1672094832</pqid></control><display><type>article</type><title>High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Woolcott, Orison O ; Richey, Joyce M ; Kabir, Morvarid ; Chow, Robert H ; Iyer, Malini S ; Kirkman, Erlinda L ; Stefanovski, Darko ; Lottati, Maya ; Kim, Stella P ; Harrison, L Nicole ; Ionut, Viorica ; Zheng, Dan ; Hsu, Isabel R ; Catalano, Karyn J ; Chiu, Jenny D ; Bradshaw, Heather ; Wu, Qiang ; Kolka, Cathryn M ; Bergman, Richard N</creator><contributor>Alquier, Thierry</contributor><creatorcontrib>Woolcott, Orison O ; Richey, Joyce M ; Kabir, Morvarid ; Chow, Robert H ; Iyer, Malini S ; Kirkman, Erlinda L ; Stefanovski, Darko ; Lottati, Maya ; Kim, Stella P ; Harrison, L Nicole ; Ionut, Viorica ; Zheng, Dan ; Hsu, Isabel R ; Catalano, Karyn J ; Chiu, Jenny D ; Bradshaw, Heather ; Wu, Qiang ; Kolka, Cathryn M ; Bergman, Richard N ; Alquier, Thierry</creatorcontrib><description>Obesity has been associated with elevated plasma anandamide levels. In addition, anandamide has been shown to stimulate insulin secretion in vitro, suggesting that anandamide might be linked to hyperinsulinemia.
To determine whether high-fat diet-induced insulin resistance increases anandamide levels and potentiates the insulinotropic effect of anandamide in isolated pancreatic islets.
Dogs were fed a high-fat diet (n = 9) for 22 weeks. Abdominal fat depot was quantified by MRI. Insulin sensitivity was assessed by the euglycemic-hyperinsulinemic clamp. Fasting plasma endocannabinoid levels were analyzed by liquid chromatography-mass spectrometry. All metabolic assessments were performed before and after fat diet regimen. At the end of the study, pancreatic islets were isolated prior to euthanasia to test the in vitro effect of anandamide on islet hormones. mRNA expression of cannabinoid receptors was determined in intact islets. The findings in vitro were compared with those from animals fed a control diet (n = 7).
Prolonged fat feeding increased abdominal fat content by 81.3±21.6% (mean±S.E.M, P<0.01). In vivo insulin sensitivity decreased by 31.3±12.1% (P<0.05), concomitant with a decrease in plasma 2-arachidonoyl glycerol (from 39.1±5.2 to 15.7±2.0 nmol/L) but not anandamide, oleoyl ethanolamide, linoleoyl ethanolamide, or palmitoyl ethanolamide. In control-diet animals (body weight: 28.8±1.0 kg), islets incubated with anandamide had a higher basal and glucose-stimulated insulin secretion as compared with no treatment. Islets from fat-fed animals (34.5±1.3 kg; P<0.05 versus control) did not exhibit further potentiation of anandamide-induced insulin secretion as compared with control-diet animals. Glucagon but not somatostatin secretion in vitro was also increased in response to anandamide, but there was no difference between groups (P = 0.705). No differences in gene expression of CB1R or CB2R between groups were found.
In canines, high-fat diet-induced insulin resistance does not alter plasma anandamide levels or further potentiate the insulinotropic effect of anandamide in vitro.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0123558</identifier><identifier>PMID: 25855974</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdominal Fat - drug effects ; Abdominal Fat - metabolism ; Analysis ; Anandamide ; Animals ; Antimicrobial Cationic Peptides - biosynthesis ; Arachidonic Acids - blood ; Arachidonic Acids - genetics ; Blood Glucose ; Body Weight ; Cannabinoid CB1 receptors ; Cannabinoid CB2 receptors ; Chromatography ; Diet ; Diet, High-Fat - adverse effects ; Dogs ; Endocannabinoids - blood ; Endocannabinoids - genetics ; Euthanasia ; Gene expression ; Glucagon ; Glucose ; Glycerol ; High fat diet ; Hormones ; Humans ; Hyperinsulinemia ; In vitro methods and tests ; In vivo methods and tests ; Insulin ; Insulin - metabolism ; Insulin Resistance ; Insulin secretion ; Islets of Langerhans ; Islets of Langerhans - metabolism ; Islets of Langerhans - pathology ; Kinases ; Liquid chromatography ; Magnetic resonance imaging ; Mass spectrometry ; Mass spectroscopy ; Obesity ; Obesity - blood ; Obesity - pathology ; Pancreas ; Physicians ; Physiological aspects ; Polyunsaturated Alkamides - blood ; Potentiation ; Receptor, Cannabinoid, CB2 - biosynthesis ; Receptors ; Rodents ; Secretion ; Sensitivity ; Sensitivity analysis ; Somatostatin</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0123558-e0123558</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Woolcott et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Woolcott et al 2015 Woolcott et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-6553208da24b1f3f46637f864621f6ecb44160fdd6b2500a5d6d630c303ccb6d3</citedby><cites>FETCH-LOGICAL-c692t-6553208da24b1f3f46637f864621f6ecb44160fdd6b2500a5d6d630c303ccb6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391925/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391925/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2097,2916,23848,27906,27907,53773,53775,79350,79351</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25855974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Alquier, Thierry</contributor><creatorcontrib>Woolcott, Orison O</creatorcontrib><creatorcontrib>Richey, Joyce M</creatorcontrib><creatorcontrib>Kabir, Morvarid</creatorcontrib><creatorcontrib>Chow, Robert H</creatorcontrib><creatorcontrib>Iyer, Malini S</creatorcontrib><creatorcontrib>Kirkman, Erlinda L</creatorcontrib><creatorcontrib>Stefanovski, Darko</creatorcontrib><creatorcontrib>Lottati, Maya</creatorcontrib><creatorcontrib>Kim, Stella P</creatorcontrib><creatorcontrib>Harrison, L Nicole</creatorcontrib><creatorcontrib>Ionut, Viorica</creatorcontrib><creatorcontrib>Zheng, Dan</creatorcontrib><creatorcontrib>Hsu, Isabel R</creatorcontrib><creatorcontrib>Catalano, Karyn J</creatorcontrib><creatorcontrib>Chiu, Jenny D</creatorcontrib><creatorcontrib>Bradshaw, Heather</creatorcontrib><creatorcontrib>Wu, Qiang</creatorcontrib><creatorcontrib>Kolka, Cathryn M</creatorcontrib><creatorcontrib>Bergman, Richard N</creatorcontrib><title>High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Obesity has been associated with elevated plasma anandamide levels. In addition, anandamide has been shown to stimulate insulin secretion in vitro, suggesting that anandamide might be linked to hyperinsulinemia.
To determine whether high-fat diet-induced insulin resistance increases anandamide levels and potentiates the insulinotropic effect of anandamide in isolated pancreatic islets.
Dogs were fed a high-fat diet (n = 9) for 22 weeks. Abdominal fat depot was quantified by MRI. Insulin sensitivity was assessed by the euglycemic-hyperinsulinemic clamp. Fasting plasma endocannabinoid levels were analyzed by liquid chromatography-mass spectrometry. All metabolic assessments were performed before and after fat diet regimen. At the end of the study, pancreatic islets were isolated prior to euthanasia to test the in vitro effect of anandamide on islet hormones. mRNA expression of cannabinoid receptors was determined in intact islets. The findings in vitro were compared with those from animals fed a control diet (n = 7).
Prolonged fat feeding increased abdominal fat content by 81.3±21.6% (mean±S.E.M, P<0.01). In vivo insulin sensitivity decreased by 31.3±12.1% (P<0.05), concomitant with a decrease in plasma 2-arachidonoyl glycerol (from 39.1±5.2 to 15.7±2.0 nmol/L) but not anandamide, oleoyl ethanolamide, linoleoyl ethanolamide, or palmitoyl ethanolamide. In control-diet animals (body weight: 28.8±1.0 kg), islets incubated with anandamide had a higher basal and glucose-stimulated insulin secretion as compared with no treatment. Islets from fat-fed animals (34.5±1.3 kg; P<0.05 versus control) did not exhibit further potentiation of anandamide-induced insulin secretion as compared with control-diet animals. Glucagon but not somatostatin secretion in vitro was also increased in response to anandamide, but there was no difference between groups (P = 0.705). No differences in gene expression of CB1R or CB2R between groups were found.
In canines, high-fat diet-induced insulin resistance does not alter plasma anandamide levels or further potentiate the insulinotropic effect of anandamide in vitro.</description><subject>Abdominal Fat - drug effects</subject><subject>Abdominal Fat - metabolism</subject><subject>Analysis</subject><subject>Anandamide</subject><subject>Animals</subject><subject>Antimicrobial Cationic Peptides - biosynthesis</subject><subject>Arachidonic Acids - blood</subject><subject>Arachidonic Acids - genetics</subject><subject>Blood Glucose</subject><subject>Body Weight</subject><subject>Cannabinoid CB1 receptors</subject><subject>Cannabinoid CB2 receptors</subject><subject>Chromatography</subject><subject>Diet</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Dogs</subject><subject>Endocannabinoids - blood</subject><subject>Endocannabinoids - genetics</subject><subject>Euthanasia</subject><subject>Gene expression</subject><subject>Glucagon</subject><subject>Glucose</subject><subject>Glycerol</subject><subject>High fat diet</subject><subject>Hormones</subject><subject>Humans</subject><subject>Hyperinsulinemia</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin Resistance</subject><subject>Insulin secretion</subject><subject>Islets of Langerhans</subject><subject>Islets of Langerhans - metabolism</subject><subject>Islets of Langerhans - pathology</subject><subject>Kinases</subject><subject>Liquid chromatography</subject><subject>Magnetic resonance imaging</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Obesity</subject><subject>Obesity - blood</subject><subject>Obesity - pathology</subject><subject>Pancreas</subject><subject>Physicians</subject><subject>Physiological aspects</subject><subject>Polyunsaturated Alkamides - blood</subject><subject>Potentiation</subject><subject>Receptor, Cannabinoid, CB2 - biosynthesis</subject><subject>Receptors</subject><subject>Rodents</subject><subject>Secretion</subject><subject>Sensitivity</subject><subject>Sensitivity analysis</subject><subject>Somatostatin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1trFDEUxwdRbK1-A9EBQfRh19xn5kUoRW2hUPD2GrLJyW5KNlmTTNHv4oc1607LrvRB8pCQ8zv_5Nya5jlGc0w7_O46jikoP9_EAHOECeW8f9Ac44GSmSCIPtw7HzVPcr5GiNNeiMfNEeE950PHjpvf5265mllVWuOgzFwwowbTupBH70KbILtcVNDQmgi5DbFUm06gMrQbr_JatSqoYNTaGWg93IDPbUztJhYIxakC-_ZJNpYUN063YC3orWDrcvSVNa1WwYUKZg8lP20eWeUzPJv2k-bbxw9fz85nl1efLs5OL2daDKTMBOeUoN4owhbYUsuEoJ3tBRMEWwF6wRgWyBojFoQjpLgRRlCkKaJaL4ShJ83Lne7GxyynxGaJRUfQwHpKKnGxI0xU13KT3FqlXzIqJ_9exLSUKhWnPUg1cI4YGxYCY4ZA952h0PVsIFYBs6hqvZ9eGxdrMLrmKSl_IHpoCW4ll_FGMjrggfAq8GYSSPHHCLnItcsavFcB4rj7t8C0fruir_5B749uopaqBuCCrQVSeisqTxmpTdQT2lVqfg9Vl4G107ULrav3Bw5vDxwqU-BnWaoxZ3nx5fP_s1ffD9nXe-wKlC-r2j9jcTHkQ5DtQJ1izgnsXZIxktshus2G3A6RnIaour3YL9Cd0-3U0D9Z5hmp</recordid><startdate>20150409</startdate><enddate>20150409</enddate><creator>Woolcott, Orison O</creator><creator>Richey, Joyce M</creator><creator>Kabir, Morvarid</creator><creator>Chow, Robert H</creator><creator>Iyer, Malini S</creator><creator>Kirkman, Erlinda L</creator><creator>Stefanovski, Darko</creator><creator>Lottati, Maya</creator><creator>Kim, Stella P</creator><creator>Harrison, L Nicole</creator><creator>Ionut, Viorica</creator><creator>Zheng, Dan</creator><creator>Hsu, Isabel R</creator><creator>Catalano, Karyn J</creator><creator>Chiu, Jenny D</creator><creator>Bradshaw, Heather</creator><creator>Wu, Qiang</creator><creator>Kolka, Cathryn M</creator><creator>Bergman, Richard N</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150409</creationdate><title>High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets</title><author>Woolcott, Orison O ; Richey, Joyce M ; Kabir, Morvarid ; Chow, Robert H ; Iyer, Malini S ; Kirkman, Erlinda L ; Stefanovski, Darko ; Lottati, Maya ; Kim, Stella P ; Harrison, L Nicole ; Ionut, Viorica ; Zheng, Dan ; Hsu, Isabel R ; Catalano, Karyn J ; Chiu, Jenny D ; Bradshaw, Heather ; Wu, Qiang ; Kolka, Cathryn M ; Bergman, Richard N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6553208da24b1f3f46637f864621f6ecb44160fdd6b2500a5d6d630c303ccb6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abdominal Fat - drug effects</topic><topic>Abdominal Fat - metabolism</topic><topic>Analysis</topic><topic>Anandamide</topic><topic>Animals</topic><topic>Antimicrobial Cationic Peptides - biosynthesis</topic><topic>Arachidonic Acids - blood</topic><topic>Arachidonic Acids - genetics</topic><topic>Blood Glucose</topic><topic>Body Weight</topic><topic>Cannabinoid CB1 receptors</topic><topic>Cannabinoid CB2 receptors</topic><topic>Chromatography</topic><topic>Diet</topic><topic>Diet, High-Fat - adverse effects</topic><topic>Dogs</topic><topic>Endocannabinoids - blood</topic><topic>Endocannabinoids - genetics</topic><topic>Euthanasia</topic><topic>Gene expression</topic><topic>Glucagon</topic><topic>Glucose</topic><topic>Glycerol</topic><topic>High fat diet</topic><topic>Hormones</topic><topic>Humans</topic><topic>Hyperinsulinemia</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Insulin Resistance</topic><topic>Insulin secretion</topic><topic>Islets of Langerhans</topic><topic>Islets of Langerhans - metabolism</topic><topic>Islets of Langerhans - pathology</topic><topic>Kinases</topic><topic>Liquid chromatography</topic><topic>Magnetic resonance imaging</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Obesity</topic><topic>Obesity - blood</topic><topic>Obesity - pathology</topic><topic>Pancreas</topic><topic>Physicians</topic><topic>Physiological aspects</topic><topic>Polyunsaturated Alkamides - blood</topic><topic>Potentiation</topic><topic>Receptor, Cannabinoid, CB2 - biosynthesis</topic><topic>Receptors</topic><topic>Rodents</topic><topic>Secretion</topic><topic>Sensitivity</topic><topic>Sensitivity analysis</topic><topic>Somatostatin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woolcott, Orison O</creatorcontrib><creatorcontrib>Richey, Joyce M</creatorcontrib><creatorcontrib>Kabir, Morvarid</creatorcontrib><creatorcontrib>Chow, Robert H</creatorcontrib><creatorcontrib>Iyer, Malini S</creatorcontrib><creatorcontrib>Kirkman, Erlinda L</creatorcontrib><creatorcontrib>Stefanovski, Darko</creatorcontrib><creatorcontrib>Lottati, Maya</creatorcontrib><creatorcontrib>Kim, Stella P</creatorcontrib><creatorcontrib>Harrison, L Nicole</creatorcontrib><creatorcontrib>Ionut, Viorica</creatorcontrib><creatorcontrib>Zheng, Dan</creatorcontrib><creatorcontrib>Hsu, Isabel R</creatorcontrib><creatorcontrib>Catalano, Karyn J</creatorcontrib><creatorcontrib>Chiu, Jenny D</creatorcontrib><creatorcontrib>Bradshaw, Heather</creatorcontrib><creatorcontrib>Wu, Qiang</creatorcontrib><creatorcontrib>Kolka, Cathryn M</creatorcontrib><creatorcontrib>Bergman, Richard N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woolcott, Orison O</au><au>Richey, Joyce M</au><au>Kabir, Morvarid</au><au>Chow, Robert H</au><au>Iyer, Malini S</au><au>Kirkman, Erlinda L</au><au>Stefanovski, Darko</au><au>Lottati, Maya</au><au>Kim, Stella P</au><au>Harrison, L Nicole</au><au>Ionut, Viorica</au><au>Zheng, Dan</au><au>Hsu, Isabel R</au><au>Catalano, Karyn J</au><au>Chiu, Jenny D</au><au>Bradshaw, Heather</au><au>Wu, Qiang</au><au>Kolka, Cathryn M</au><au>Bergman, Richard N</au><au>Alquier, Thierry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-04-09</date><risdate>2015</risdate><volume>10</volume><issue>4</issue><spage>e0123558</spage><epage>e0123558</epage><pages>e0123558-e0123558</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Obesity has been associated with elevated plasma anandamide levels. In addition, anandamide has been shown to stimulate insulin secretion in vitro, suggesting that anandamide might be linked to hyperinsulinemia.
To determine whether high-fat diet-induced insulin resistance increases anandamide levels and potentiates the insulinotropic effect of anandamide in isolated pancreatic islets.
Dogs were fed a high-fat diet (n = 9) for 22 weeks. Abdominal fat depot was quantified by MRI. Insulin sensitivity was assessed by the euglycemic-hyperinsulinemic clamp. Fasting plasma endocannabinoid levels were analyzed by liquid chromatography-mass spectrometry. All metabolic assessments were performed before and after fat diet regimen. At the end of the study, pancreatic islets were isolated prior to euthanasia to test the in vitro effect of anandamide on islet hormones. mRNA expression of cannabinoid receptors was determined in intact islets. The findings in vitro were compared with those from animals fed a control diet (n = 7).
Prolonged fat feeding increased abdominal fat content by 81.3±21.6% (mean±S.E.M, P<0.01). In vivo insulin sensitivity decreased by 31.3±12.1% (P<0.05), concomitant with a decrease in plasma 2-arachidonoyl glycerol (from 39.1±5.2 to 15.7±2.0 nmol/L) but not anandamide, oleoyl ethanolamide, linoleoyl ethanolamide, or palmitoyl ethanolamide. In control-diet animals (body weight: 28.8±1.0 kg), islets incubated with anandamide had a higher basal and glucose-stimulated insulin secretion as compared with no treatment. Islets from fat-fed animals (34.5±1.3 kg; P<0.05 versus control) did not exhibit further potentiation of anandamide-induced insulin secretion as compared with control-diet animals. Glucagon but not somatostatin secretion in vitro was also increased in response to anandamide, but there was no difference between groups (P = 0.705). No differences in gene expression of CB1R or CB2R between groups were found.
In canines, high-fat diet-induced insulin resistance does not alter plasma anandamide levels or further potentiate the insulinotropic effect of anandamide in vitro.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25855974</pmid><doi>10.1371/journal.pone.0123558</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-04, Vol.10 (4), p.e0123558-e0123558 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1672094832 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abdominal Fat - drug effects Abdominal Fat - metabolism Analysis Anandamide Animals Antimicrobial Cationic Peptides - biosynthesis Arachidonic Acids - blood Arachidonic Acids - genetics Blood Glucose Body Weight Cannabinoid CB1 receptors Cannabinoid CB2 receptors Chromatography Diet Diet, High-Fat - adverse effects Dogs Endocannabinoids - blood Endocannabinoids - genetics Euthanasia Gene expression Glucagon Glucose Glycerol High fat diet Hormones Humans Hyperinsulinemia In vitro methods and tests In vivo methods and tests Insulin Insulin - metabolism Insulin Resistance Insulin secretion Islets of Langerhans Islets of Langerhans - metabolism Islets of Langerhans - pathology Kinases Liquid chromatography Magnetic resonance imaging Mass spectrometry Mass spectroscopy Obesity Obesity - blood Obesity - pathology Pancreas Physicians Physiological aspects Polyunsaturated Alkamides - blood Potentiation Receptor, Cannabinoid, CB2 - biosynthesis Receptors Rodents Secretion Sensitivity Sensitivity analysis Somatostatin |
| title | High-fat diet-induced insulin resistance does not increase plasma anandamide levels or potentiate anandamide insulinotropic effect in isolated canine islets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T09%3A40%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-fat%20diet-induced%20insulin%20resistance%20does%20not%20increase%20plasma%20anandamide%20levels%20or%20potentiate%20anandamide%20insulinotropic%20effect%20in%20isolated%20canine%20islets&rft.jtitle=PloS%20one&rft.au=Woolcott,%20Orison%20O&rft.date=2015-04-09&rft.volume=10&rft.issue=4&rft.spage=e0123558&rft.epage=e0123558&rft.pages=e0123558-e0123558&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0123558&rft_dat=%3Cgale_plos_%3EA429328237%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1672094832&rft_id=info:pmid/25855974&rft_galeid=A429328237&rft_doaj_id=oai_doaj_org_article_a9550449b61140ec87d3e78492fae4f0&rfr_iscdi=true |