Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens)
Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including t...
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| Veröffentlicht in: | PloS one 2015-04, Vol.10 (4), p.e0121127-e0121127 |
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| creator | Price, Ben W Henry, Charles S Hall, Andie C Mochizuki, Atsushi Duelli, Peter Brooks, Stephen J |
| description | Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea. |
| doi_str_mv | 10.1371/journal.pone.0121127 |
| format | Article |
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The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0121127</identifier><identifier>PMID: 25853856</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Arable land ; Bayes Theorem ; Bayesian analysis ; Biodiversity ; Biological control ; Chrysoperla carnea ; Chrysopidae ; Courtship ; Cryptic species ; Data collection ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; DNA - genetics ; DNA sequencing ; Gene sequencing ; High-Throughput Nucleotide Sequencing ; Historical account ; Insecta - genetics ; Larva - genetics ; Lectotypes ; Molecular Sequence Data ; Neuroptera ; Phylogeny ; Physiological aspects ; Repositories ; Singing ; Species Specificity ; Specimen Handling</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0121127-e0121127</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Price et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Price et al 2015 Price et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d28d5d28147ac351a5cb5fbd122240c75a4e0051c38491584a1a9e12a2ee97ef3</citedby><cites>FETCH-LOGICAL-c692t-d28d5d28147ac351a5cb5fbd122240c75a4e0051c38491584a1a9e12a2ee97ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390323/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390323/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25853856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Steinke, Dirk</contributor><creatorcontrib>Price, Ben W</creatorcontrib><creatorcontrib>Henry, Charles S</creatorcontrib><creatorcontrib>Hall, Andie C</creatorcontrib><creatorcontrib>Mochizuki, Atsushi</creatorcontrib><creatorcontrib>Duelli, Peter</creatorcontrib><creatorcontrib>Brooks, Stephen J</creatorcontrib><title>Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.</description><subject>Analysis</subject><subject>Animals</subject><subject>Arable land</subject><subject>Bayes Theorem</subject><subject>Bayesian analysis</subject><subject>Biodiversity</subject><subject>Biological control</subject><subject>Chrysoperla carnea</subject><subject>Chrysopidae</subject><subject>Courtship</subject><subject>Cryptic species</subject><subject>Data collection</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA sequencing</subject><subject>Gene sequencing</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Historical account</subject><subject>Insecta - genetics</subject><subject>Larva - genetics</subject><subject>Lectotypes</subject><subject>Molecular Sequence Data</subject><subject>Neuroptera</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Repositories</subject><subject>Singing</subject><subject>Species Specificity</subject><subject>Specimen Handling</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7jr6D0QDguxezNgk_fRCGMavgcUFR70Np-lpJ0vb1CRdnH9vZqa7TGUvpKUNJ895T84bThC8pOGC8pS-u9GD6aBZ9LrDRUgZpSx9FJzTnLN5wkL--GR9Fjyz9iYMY54lydPgjMWZX8bJeeA2qqv9SyqjW-K2SGoDt_iefPy2PMaA0CwkOwRDdFMSt-uR2B6larEjUneVMq09ZKoSO6fcjuiKrLZmZ3WPpgEiwXQI5GLjsN9iZy-fB08qaCy-GP-z4OfnTz9WX-dX11_Wq-XVXCY5c_OSZWXsPzRKQfKYQiyLuCpKyhiLQpnGEKHviUqeRTmNswgo5EgZMMQ8xYrPgtdH3b7RVoyGWUGTlLIwo96eWbA-EqWGG9Eb1YLZCQ1KHALa1AKMU7JBgRX68jHPvXMRY7zgqayKosjyLIUKYq_1Yaw2FC2W0pthoJmITnc6tRW1vhURz0POuBe4GAWM_j2gdaJVVmLTQId6OJybJWEWh5lH3_yDPtzdSNXgG1BdpX1duRcVy4jlPI14mnpq8QDlnxJb5S8YK-Xjk4TLSYJnHP5xNQzWivXm-_-z17-m7NsTdovQuK3VzeCU7uwUjI6gNNpag9W9yTQU--G4c0Psh0OMw-HTXp1e0H3S3TTwvw82CJ8</recordid><startdate>20150408</startdate><enddate>20150408</enddate><creator>Price, Ben W</creator><creator>Henry, Charles S</creator><creator>Hall, Andie C</creator><creator>Mochizuki, Atsushi</creator><creator>Duelli, Peter</creator><creator>Brooks, Stephen J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150408</creationdate><title>Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens)</title><author>Price, Ben W ; Henry, Charles S ; Hall, Andie C ; Mochizuki, Atsushi ; Duelli, Peter ; Brooks, Stephen J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d28d5d28147ac351a5cb5fbd122240c75a4e0051c38491584a1a9e12a2ee97ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Arable land</topic><topic>Bayes Theorem</topic><topic>Bayesian analysis</topic><topic>Biodiversity</topic><topic>Biological control</topic><topic>Chrysoperla carnea</topic><topic>Chrysopidae</topic><topic>Courtship</topic><topic>Cryptic species</topic><topic>Data collection</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnostic systems</topic><topic>DNA</topic><topic>DNA - 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The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25853856</pmid><doi>10.1371/journal.pone.0121127</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Arable land Bayes Theorem Bayesian analysis Biodiversity Biological control Chrysoperla carnea Chrysopidae Courtship Cryptic species Data collection Deoxyribonucleic acid Diagnostic systems DNA DNA - genetics DNA sequencing Gene sequencing High-Throughput Nucleotide Sequencing Historical account Insecta - genetics Larva - genetics Lectotypes Molecular Sequence Data Neuroptera Phylogeny Physiological aspects Repositories Singing Species Specificity Specimen Handling |
| title | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens) |
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