Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties
Osteoarthritis (OA) is associated with a local inflammatory process. Dyslipidemia is known to be an underlying cause for the development of OA. Therefore, lipid and inflammatory levels were quantified ex vivo in blood and synovial fluid of OA patients (n=29) and compared to those of rheumatoid arthr...
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| Veröffentlicht in: | PloS one 2015-04, Vol.10 (4), p.e0122904 |
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| creator | de Seny, Dominique Cobraiville, Gaël Charlier, Edith Neuville, Sophie Lutteri, Laurence Le Goff, Caroline Malaise, Denis Malaise, Olivier Chapelle, Jean-Paul Relic, Biserka Malaise, Michel G |
| description | Osteoarthritis (OA) is associated with a local inflammatory process. Dyslipidemia is known to be an underlying cause for the development of OA. Therefore, lipid and inflammatory levels were quantified ex vivo in blood and synovial fluid of OA patients (n=29) and compared to those of rheumatoid arthritis (RA) patients (n=27) or healthy volunteers (HV) (n=35). The role of apolipoprotein A-I (ApoA1) was investigated in vitro on inflammatory parameters using human joint cells isolated from cartilage and synovial membrane obtained from OA patients after joint replacement. Cells were stimulated with ApoA1 in the presence or not of serum amyloid A (SAA) protein and/or lipoproteins (LDL and HDL) at physiological concentration observed in OA synovial fluid. In our ex vivo study, ApoA1, LDL-C and total cholesterol levels were strongly correlated to each other inside the OA joint cavity whereas same levels were not or weakly correlated to their corresponding serum levels. In OA synovial fluid, ApoA1 was not as strongly correlated to HDL as observed in OA serum or in RA synovial fluid, suggesting a dissociative level between ApoA1 and HDL in OA synovial fluid. In vitro, ApoA1 induced IL-6, MMP-1 and MMP-3 expression by primary chondrocytes and fibroblast-like synoviocytes through TLR4 receptor. HDL and LDL attenuated joint inflammatory response induced by ApoA1 and SAA in a ratio dependent manner. In conclusion, a dysregulated lipidic profile in the synovial fluid of OA patients was observed and was correlated with inflammatory parameters in the OA joint cavity. Pro-inflammatory properties of ApoA1 were confirmed in vitro. |
| doi_str_mv | 10.1371/journal.pone.0122904 |
| format | Article |
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Dyslipidemia is known to be an underlying cause for the development of OA. Therefore, lipid and inflammatory levels were quantified ex vivo in blood and synovial fluid of OA patients (n=29) and compared to those of rheumatoid arthritis (RA) patients (n=27) or healthy volunteers (HV) (n=35). The role of apolipoprotein A-I (ApoA1) was investigated in vitro on inflammatory parameters using human joint cells isolated from cartilage and synovial membrane obtained from OA patients after joint replacement. Cells were stimulated with ApoA1 in the presence or not of serum amyloid A (SAA) protein and/or lipoproteins (LDL and HDL) at physiological concentration observed in OA synovial fluid. In our ex vivo study, ApoA1, LDL-C and total cholesterol levels were strongly correlated to each other inside the OA joint cavity whereas same levels were not or weakly correlated to their corresponding serum levels. In OA synovial fluid, ApoA1 was not as strongly correlated to HDL as observed in OA serum or in RA synovial fluid, suggesting a dissociative level between ApoA1 and HDL in OA synovial fluid. In vitro, ApoA1 induced IL-6, MMP-1 and MMP-3 expression by primary chondrocytes and fibroblast-like synoviocytes through TLR4 receptor. HDL and LDL attenuated joint inflammatory response induced by ApoA1 and SAA in a ratio dependent manner. In conclusion, a dysregulated lipidic profile in the synovial fluid of OA patients was observed and was correlated with inflammatory parameters in the OA joint cavity. Pro-inflammatory properties of ApoA1 were confirmed in vitro.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0122904</identifier><identifier>PMID: 25849372</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Amyloid ; Apolipoprotein A ; Apolipoprotein A-I ; Apolipoprotein A-I - metabolism ; Apolipoprotein A1 ; Apolipoproteins ; Arthritis ; Atherosclerosis ; Biocompatibility ; Biomedical materials ; Blood lipids ; Cardiology ; Cardiovascular disease ; Cartilage ; Cartilage diseases ; Cells, Cultured ; Cholesterol ; Chondrocytes ; Chondrocytes - immunology ; Chondrocytes - metabolism ; Comparative analysis ; Damage patterns ; DAMP ; Diabetes ; Dyslipidemia ; Extracellular matrix ; Female ; Fibroblasts ; High density lipoprotein ; Human health sciences ; Humans ; Inflammation ; Inflammatory response ; Interleukin 6 ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Interstitial collagenase ; Laboratories ; Lipids ; Lipopolysaccharides - pharmacology ; Lipoproteins ; Low density lipoprotein ; Low density lipoproteins ; Male ; Matrix metalloproteinase ; Matrix Metalloproteinase 1 - metabolism ; Matrix Metalloproteinase 3 - metabolism ; Metabolism ; Middle Aged ; Obesity ; Osteoarthritis ; Osteoarthritis - immunology ; Osteoarthritis - metabolism ; Patients ; Physiological aspects ; Primary Cell Culture ; Proteins ; Rheumatoid arthritis ; Rheumatoid factor ; Rheumatology ; Rhumatologie ; Sciences de la santé humaine ; Serum levels ; Studies ; Synovial fluid ; Synovial Fluid - metabolism ; Synovial membrane ; Synoviocytes ; TLR4 protein ; Toll-Like Receptor 4 - metabolism ; Toll-like receptors ; Transcriptional Activation ; Young Adult</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0122904</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 de Seny et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 de Seny et al 2015 de Seny et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c736t-3952679be552619394262e8a5ef559ed915f963c15e67b2632b9b1fa6c3deec3</citedby><cites>FETCH-LOGICAL-c736t-3952679be552619394262e8a5ef559ed915f963c15e67b2632b9b1fa6c3deec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388661/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388661/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25849372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Feng, Yingmei</contributor><creatorcontrib>de Seny, Dominique</creatorcontrib><creatorcontrib>Cobraiville, Gaël</creatorcontrib><creatorcontrib>Charlier, Edith</creatorcontrib><creatorcontrib>Neuville, Sophie</creatorcontrib><creatorcontrib>Lutteri, Laurence</creatorcontrib><creatorcontrib>Le Goff, Caroline</creatorcontrib><creatorcontrib>Malaise, Denis</creatorcontrib><creatorcontrib>Malaise, Olivier</creatorcontrib><creatorcontrib>Chapelle, Jean-Paul</creatorcontrib><creatorcontrib>Relic, Biserka</creatorcontrib><creatorcontrib>Malaise, Michel G</creatorcontrib><title>Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Osteoarthritis (OA) is associated with a local inflammatory process. Dyslipidemia is known to be an underlying cause for the development of OA. Therefore, lipid and inflammatory levels were quantified ex vivo in blood and synovial fluid of OA patients (n=29) and compared to those of rheumatoid arthritis (RA) patients (n=27) or healthy volunteers (HV) (n=35). The role of apolipoprotein A-I (ApoA1) was investigated in vitro on inflammatory parameters using human joint cells isolated from cartilage and synovial membrane obtained from OA patients after joint replacement. Cells were stimulated with ApoA1 in the presence or not of serum amyloid A (SAA) protein and/or lipoproteins (LDL and HDL) at physiological concentration observed in OA synovial fluid. In our ex vivo study, ApoA1, LDL-C and total cholesterol levels were strongly correlated to each other inside the OA joint cavity whereas same levels were not or weakly correlated to their corresponding serum levels. In OA synovial fluid, ApoA1 was not as strongly correlated to HDL as observed in OA serum or in RA synovial fluid, suggesting a dissociative level between ApoA1 and HDL in OA synovial fluid. In vitro, ApoA1 induced IL-6, MMP-1 and MMP-3 expression by primary chondrocytes and fibroblast-like synoviocytes through TLR4 receptor. HDL and LDL attenuated joint inflammatory response induced by ApoA1 and SAA in a ratio dependent manner. In conclusion, a dysregulated lipidic profile in the synovial fluid of OA patients was observed and was correlated with inflammatory parameters in the OA joint cavity. Pro-inflammatory properties of ApoA1 were confirmed in vitro.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amyloid</subject><subject>Apolipoprotein A</subject><subject>Apolipoprotein A-I</subject><subject>Apolipoprotein A-I - metabolism</subject><subject>Apolipoprotein A1</subject><subject>Apolipoproteins</subject><subject>Arthritis</subject><subject>Atherosclerosis</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Blood lipids</subject><subject>Cardiology</subject><subject>Cardiovascular disease</subject><subject>Cartilage</subject><subject>Cartilage diseases</subject><subject>Cells, Cultured</subject><subject>Cholesterol</subject><subject>Chondrocytes</subject><subject>Chondrocytes - immunology</subject><subject>Chondrocytes - metabolism</subject><subject>Comparative analysis</subject><subject>Damage patterns</subject><subject>DAMP</subject><subject>Diabetes</subject><subject>Dyslipidemia</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>High density lipoprotein</subject><subject>Human health sciences</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Interstitial collagenase</subject><subject>Laboratories</subject><subject>Lipids</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lipoproteins</subject><subject>Low density lipoprotein</subject><subject>Low density lipoproteins</subject><subject>Male</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Matrix Metalloproteinase 3 - metabolism</subject><subject>Metabolism</subject><subject>Middle Aged</subject><subject>Obesity</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - immunology</subject><subject>Osteoarthritis - metabolism</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Primary Cell Culture</subject><subject>Proteins</subject><subject>Rheumatoid arthritis</subject><subject>Rheumatoid factor</subject><subject>Rheumatology</subject><subject>Rhumatologie</subject><subject>Sciences de la santé humaine</subject><subject>Serum levels</subject><subject>Studies</subject><subject>Synovial fluid</subject><subject>Synovial Fluid - metabolism</subject><subject>Synovial membrane</subject><subject>Synoviocytes</subject><subject>TLR4 protein</subject><subject>Toll-Like Receptor 4 - metabolism</subject><subject>Toll-like receptors</subject><subject>Transcriptional Activation</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk92K1DAUx4so7rr6BqIFQfSiY5O0aeOFMCx-DCws6OJtSNPTTpa06SbpsPsEvrbpTHeZyl5IAwknv__5yOmJotcoXSFSoE_XZrS90KvB9LBKEcYszZ5Ep4gRnFCckqdH55PohXPXaZqTktLn0QnOy4yRAp9Gf9aD0WowgzUeVJ-sUSxcLOJadKKFRDhnpBIe6rgzGuSohY0H4T3Y3sWzKA7LOA9GWL-1yiv3OYbbeKd2JhZ9PV3vlLdm4hPVN1p0nfDG3k2GAaxX4F5GzxqhHbya97Po6tvXq_MfycXl9835-iKRBaE-ISzHtGAV5GEP5bEMUwylyKHJcwY1Q3nDKJEoB1pUmBJcsQo1gkpSA0hyFr09uB20cXx-QscRLVCKMlRmgdgciNqIaz5Y1Ql7x41QfG8wtuWhTCU18LIBVqSYQAaQEZqXFGFo0pRSQqsCieDryxxtrDqoJfTeCr1wurzp1Za3ZsczUoZGoeCAHBxoBS2E4JXiO7wX7s-jDtlIXgHHmJYclTgvJtWHOaw1NyM4zzvlJGgtejDjvlqcspAwDui7f9DH32SmWhHKDh00IVs5OeXrDLOiKPOCBWr1CBW-Gjolw1_aqGBfCD4uBIHxcOtbMTrHN79-_j97-XvJvj9ityC03zqjR69M75ZgdgClNc5ZaB5ag1I-Ddn9a_BpyPg8ZEH25ritD6L7qSJ_AQvIIxE</recordid><startdate>20150407</startdate><enddate>20150407</enddate><creator>de Seny, Dominique</creator><creator>Cobraiville, Gaël</creator><creator>Charlier, Edith</creator><creator>Neuville, Sophie</creator><creator>Lutteri, Laurence</creator><creator>Le Goff, Caroline</creator><creator>Malaise, Denis</creator><creator>Malaise, Olivier</creator><creator>Chapelle, Jean-Paul</creator><creator>Relic, Biserka</creator><creator>Malaise, Michel G</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>Q33</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150407</creationdate><title>Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties</title><author>de Seny, Dominique ; Cobraiville, Gaël ; Charlier, Edith ; Neuville, Sophie ; Lutteri, Laurence ; Le Goff, Caroline ; Malaise, Denis ; Malaise, Olivier ; Chapelle, Jean-Paul ; Relic, Biserka ; Malaise, Michel G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c736t-3952679be552619394262e8a5ef559ed915f963c15e67b2632b9b1fa6c3deec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amyloid</topic><topic>Apolipoprotein A</topic><topic>Apolipoprotein A-I</topic><topic>Apolipoprotein A-I - metabolism</topic><topic>Apolipoprotein A1</topic><topic>Apolipoproteins</topic><topic>Arthritis</topic><topic>Atherosclerosis</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Blood lipids</topic><topic>Cardiology</topic><topic>Cardiovascular disease</topic><topic>Cartilage</topic><topic>Cartilage diseases</topic><topic>Cells, Cultured</topic><topic>Cholesterol</topic><topic>Chondrocytes</topic><topic>Chondrocytes - immunology</topic><topic>Chondrocytes - metabolism</topic><topic>Comparative analysis</topic><topic>Damage patterns</topic><topic>DAMP</topic><topic>Diabetes</topic><topic>Dyslipidemia</topic><topic>Extracellular matrix</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>High density lipoprotein</topic><topic>Human health sciences</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 6</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Interstitial collagenase</topic><topic>Laboratories</topic><topic>Lipids</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lipoproteins</topic><topic>Low density lipoprotein</topic><topic>Low density lipoproteins</topic><topic>Male</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Matrix Metalloproteinase 3 - metabolism</topic><topic>Metabolism</topic><topic>Middle Aged</topic><topic>Obesity</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis - immunology</topic><topic>Osteoarthritis - metabolism</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Primary Cell Culture</topic><topic>Proteins</topic><topic>Rheumatoid arthritis</topic><topic>Rheumatoid factor</topic><topic>Rheumatology</topic><topic>Rhumatologie</topic><topic>Sciences de la santé humaine</topic><topic>Serum levels</topic><topic>Studies</topic><topic>Synovial fluid</topic><topic>Synovial Fluid - metabolism</topic><topic>Synovial membrane</topic><topic>Synoviocytes</topic><topic>TLR4 protein</topic><topic>Toll-Like Receptor 4 - metabolism</topic><topic>Toll-like receptors</topic><topic>Transcriptional Activation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Seny, Dominique</creatorcontrib><creatorcontrib>Cobraiville, Gaël</creatorcontrib><creatorcontrib>Charlier, Edith</creatorcontrib><creatorcontrib>Neuville, Sophie</creatorcontrib><creatorcontrib>Lutteri, Laurence</creatorcontrib><creatorcontrib>Le Goff, Caroline</creatorcontrib><creatorcontrib>Malaise, Denis</creatorcontrib><creatorcontrib>Malaise, Olivier</creatorcontrib><creatorcontrib>Chapelle, Jean-Paul</creatorcontrib><creatorcontrib>Relic, Biserka</creatorcontrib><creatorcontrib>Malaise, Michel G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>Université de Liège - Open Repository and Bibliography (ORBI)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Seny, Dominique</au><au>Cobraiville, Gaël</au><au>Charlier, Edith</au><au>Neuville, Sophie</au><au>Lutteri, Laurence</au><au>Le Goff, Caroline</au><au>Malaise, Denis</au><au>Malaise, Olivier</au><au>Chapelle, Jean-Paul</au><au>Relic, Biserka</au><au>Malaise, Michel G</au><au>Feng, Yingmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-04-07</date><risdate>2015</risdate><volume>10</volume><issue>4</issue><spage>e0122904</spage><pages>e0122904-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Osteoarthritis (OA) is associated with a local inflammatory process. Dyslipidemia is known to be an underlying cause for the development of OA. Therefore, lipid and inflammatory levels were quantified ex vivo in blood and synovial fluid of OA patients (n=29) and compared to those of rheumatoid arthritis (RA) patients (n=27) or healthy volunteers (HV) (n=35). The role of apolipoprotein A-I (ApoA1) was investigated in vitro on inflammatory parameters using human joint cells isolated from cartilage and synovial membrane obtained from OA patients after joint replacement. Cells were stimulated with ApoA1 in the presence or not of serum amyloid A (SAA) protein and/or lipoproteins (LDL and HDL) at physiological concentration observed in OA synovial fluid. In our ex vivo study, ApoA1, LDL-C and total cholesterol levels were strongly correlated to each other inside the OA joint cavity whereas same levels were not or weakly correlated to their corresponding serum levels. In OA synovial fluid, ApoA1 was not as strongly correlated to HDL as observed in OA serum or in RA synovial fluid, suggesting a dissociative level between ApoA1 and HDL in OA synovial fluid. In vitro, ApoA1 induced IL-6, MMP-1 and MMP-3 expression by primary chondrocytes and fibroblast-like synoviocytes through TLR4 receptor. HDL and LDL attenuated joint inflammatory response induced by ApoA1 and SAA in a ratio dependent manner. In conclusion, a dysregulated lipidic profile in the synovial fluid of OA patients was observed and was correlated with inflammatory parameters in the OA joint cavity. Pro-inflammatory properties of ApoA1 were confirmed in vitro.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25849372</pmid><doi>10.1371/journal.pone.0122904</doi><tpages>e0122904</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-04, Vol.10 (4), p.e0122904 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1671014184 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Aged, 80 and over Amyloid Apolipoprotein A Apolipoprotein A-I Apolipoprotein A-I - metabolism Apolipoprotein A1 Apolipoproteins Arthritis Atherosclerosis Biocompatibility Biomedical materials Blood lipids Cardiology Cardiovascular disease Cartilage Cartilage diseases Cells, Cultured Cholesterol Chondrocytes Chondrocytes - immunology Chondrocytes - metabolism Comparative analysis Damage patterns DAMP Diabetes Dyslipidemia Extracellular matrix Female Fibroblasts High density lipoprotein Human health sciences Humans Inflammation Inflammatory response Interleukin 6 Interleukin-6 - genetics Interleukin-6 - metabolism Interstitial collagenase Laboratories Lipids Lipopolysaccharides - pharmacology Lipoproteins Low density lipoprotein Low density lipoproteins Male Matrix metalloproteinase Matrix Metalloproteinase 1 - metabolism Matrix Metalloproteinase 3 - metabolism Metabolism Middle Aged Obesity Osteoarthritis Osteoarthritis - immunology Osteoarthritis - metabolism Patients Physiological aspects Primary Cell Culture Proteins Rheumatoid arthritis Rheumatoid factor Rheumatology Rhumatologie Sciences de la santé humaine Serum levels Studies Synovial fluid Synovial Fluid - metabolism Synovial membrane Synoviocytes TLR4 protein Toll-Like Receptor 4 - metabolism Toll-like receptors Transcriptional Activation Young Adult |
| title | Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties |
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