Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation
The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the liver of P. annectens after 6 months (the maintenance phase) of ae...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0121224-e0121224 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0121224 |
|---|---|
| container_issue | 3 |
| container_start_page | e0121224 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Hiong, Kum C Ip, Yuen K Wong, Wai P Chew, Shit F |
| description | The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the liver of P. annectens after 6 months (the maintenance phase) of aestivation as compared with the freshwater control, or after 1 day of arousal from 6 months aestivation as compared with 6 months of aestivation using suppression subtractive hybridization. During the maintenance phase of aestivation, the mRNA expression of argininosuccinate synthetase 1 and carbamoyl phosphate synthetase III were up-regulated, indicating an increase in the ornithine-urea cycle capacity to detoxify ammonia to urea. There was also an increase in the expression of betaine homocysteine-S-transferase 1 which could reduce and prevent the accumulation of hepatic homocysteine. On the other hand, the down-regulation of superoxide dismutase 1 expression could signify a decrease in ROS production during the maintenance phase of aestivation. In addition, the maintenance phase was marked by decreases in expressions of genes related to blood coagulation, complement fixation and iron and copper metabolism, which could be strategies used to prevent thrombosis and to conserve energy. Unlike the maintenance phase of aestivation, there were increases in expressions of genes related to nitrogen, carbohydrate and lipid metabolism and fatty acid transport after 1 day of arousal from 6 months aestivation. There were also up-regulation in expressions of genes that were involved in the electron transport system and ATP synthesis, indicating a greater demand for metabolic energy during arousal. Overall, our results signify the importance of sustaining a low rate of waste production and conservation of energy store during the maintenance phase, and the dependence on internal energy store for repair and structural modification during the arousal phase, of aestivation in the liver of P. annectens. |
| doi_str_mv | 10.1371/journal.pone.0121224 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1667660400</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A419036229</galeid><doaj_id>oai_doaj_org_article_4944f2784da345d79385dfd3da6eee20</doaj_id><sourcerecordid>A419036229</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-d0c9716318f93f6c6d7090de9ab284cb68f9df9b9021f80e8f657622834a042a3</originalsourceid><addsrcrecordid>eNqNk9tq3DAURU1padK0f1BaQaG0kJnqZll-KQzpLRBI6e1VaKyjGQWPNJHskHxVf7FyxglxSaH4wfbR2lvSlk5RPCd4TlhF3p2FPnrdzrfBwxwTSijlD4p9UjM6ExSzh3e-94onKZ1hXDIpxONij5aS0pLS_eL3B2ctRPCd0y1agQcEl9sIKbngkfOoWwNq3QVEFOz1z8JG12iP2t6vrEvrQ_Q1hi5sO4h9Qtp7aDrw6RBpm0tIoE3w3ToNcg2pcxe6G621y6YREWT01fVwDH3Kq7AxbP6le1o8srpN8Gx8HxQ_P338cfRldnL6-fhocTJrqlJ2M4ObuiKCEWlrZkUjTIVrbKDWSyp5sxS5bmy9rDElVmKQVpSVoFQyrjGnmh0UL3e-2zYkNWadFBGiEgJzjDNxvCNM0GdqG91GxysVtFPXhRBXSsfONS0oXnNuaSW50YyXpqqZLI01zGgBAHTwej_O1i83YJp8HFG3E9PpiHdrtQoXirNK1pRngzejQQznfY5LbVxqoG21hxzqsG5JmeBcZvTVX-j9uxuplc4bcN6GPG8zmKoFJzVmOaw6U_N7qPwY2LgmX0zrcn0ieDsRZKaDy26l-5TU8fdv_8-e_pqyr--wa9Btvjqh7Ycrk6Yg34FNDClFsLchE6yGvrpJQw19pca-yrIXdw_oVnTTSOwPGqIewA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1667660400</pqid></control><display><type>article</type><title>Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Hiong, Kum C ; Ip, Yuen K ; Wong, Wai P ; Chew, Shit F</creator><contributor>Foulkes, Nicholas S</contributor><creatorcontrib>Hiong, Kum C ; Ip, Yuen K ; Wong, Wai P ; Chew, Shit F ; Foulkes, Nicholas S</creatorcontrib><description>The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the liver of P. annectens after 6 months (the maintenance phase) of aestivation as compared with the freshwater control, or after 1 day of arousal from 6 months aestivation as compared with 6 months of aestivation using suppression subtractive hybridization. During the maintenance phase of aestivation, the mRNA expression of argininosuccinate synthetase 1 and carbamoyl phosphate synthetase III were up-regulated, indicating an increase in the ornithine-urea cycle capacity to detoxify ammonia to urea. There was also an increase in the expression of betaine homocysteine-S-transferase 1 which could reduce and prevent the accumulation of hepatic homocysteine. On the other hand, the down-regulation of superoxide dismutase 1 expression could signify a decrease in ROS production during the maintenance phase of aestivation. In addition, the maintenance phase was marked by decreases in expressions of genes related to blood coagulation, complement fixation and iron and copper metabolism, which could be strategies used to prevent thrombosis and to conserve energy. Unlike the maintenance phase of aestivation, there were increases in expressions of genes related to nitrogen, carbohydrate and lipid metabolism and fatty acid transport after 1 day of arousal from 6 months aestivation. There were also up-regulation in expressions of genes that were involved in the electron transport system and ATP synthesis, indicating a greater demand for metabolic energy during arousal. Overall, our results signify the importance of sustaining a low rate of waste production and conservation of energy store during the maintenance phase, and the dependence on internal energy store for repair and structural modification during the arousal phase, of aestivation in the liver of P. annectens.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0121224</identifier><identifier>PMID: 25822522</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptation ; Aestivation ; Africa ; Amino acids ; Ammonia ; Animals ; Argininosuccinate Synthase - metabolism ; Arousal ; Arousal - physiology ; Base Sequence ; Betaine ; Betaine-Homocysteine S-Methyltransferase - metabolism ; Blood coagulation ; Carbamoyl phosphate ; Carbohydrate metabolism ; Carbohydrates ; Carbon-Nitrogen Ligases - metabolism ; Coagulation ; Comparative analysis ; Complement fixation ; Conservation ; Copper ; Dependence ; Dipnoi ; DNA, Complementary - genetics ; Drought ; Electron transport ; Energy conservation ; Energy metabolism ; Energy Metabolism - physiology ; Estivation - physiology ; Fatty acids ; Fish ; Fishes - genetics ; Fishes - physiology ; Gene expression ; Gene Expression Regulation, Enzymologic - physiology ; Gene Library ; Gene regulation ; Genes ; Homocysteine ; Hybridization ; Internal energy ; Iron ; Ligases ; Lipid metabolism ; Liver ; Liver - metabolism ; Maintenance ; Metabolic Networks and Pathways - physiology ; Metabolism ; Molecular Sequence Data ; Ornithine ; Phosphates ; Physiology ; Proteins ; Protopterus aethiopicus ; Protopterus amphibius ; Protopterus annectens ; Protopterus dolloi ; Reactive Oxygen Species - metabolism ; Real-Time Polymerase Chain Reaction ; Reptiles & amphibians ; RNA ; Science education ; Sequence Analysis, DNA ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Thromboembolism ; Thrombosis ; Urea ; Ureas</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0121224-e0121224</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Hiong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Hiong et al 2015 Hiong et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-d0c9716318f93f6c6d7090de9ab284cb68f9df9b9021f80e8f657622834a042a3</citedby><cites>FETCH-LOGICAL-c758t-d0c9716318f93f6c6d7090de9ab284cb68f9df9b9021f80e8f657622834a042a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378924/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378924/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25822522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Foulkes, Nicholas S</contributor><creatorcontrib>Hiong, Kum C</creatorcontrib><creatorcontrib>Ip, Yuen K</creatorcontrib><creatorcontrib>Wong, Wai P</creatorcontrib><creatorcontrib>Chew, Shit F</creatorcontrib><title>Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the liver of P. annectens after 6 months (the maintenance phase) of aestivation as compared with the freshwater control, or after 1 day of arousal from 6 months aestivation as compared with 6 months of aestivation using suppression subtractive hybridization. During the maintenance phase of aestivation, the mRNA expression of argininosuccinate synthetase 1 and carbamoyl phosphate synthetase III were up-regulated, indicating an increase in the ornithine-urea cycle capacity to detoxify ammonia to urea. There was also an increase in the expression of betaine homocysteine-S-transferase 1 which could reduce and prevent the accumulation of hepatic homocysteine. On the other hand, the down-regulation of superoxide dismutase 1 expression could signify a decrease in ROS production during the maintenance phase of aestivation. In addition, the maintenance phase was marked by decreases in expressions of genes related to blood coagulation, complement fixation and iron and copper metabolism, which could be strategies used to prevent thrombosis and to conserve energy. Unlike the maintenance phase of aestivation, there were increases in expressions of genes related to nitrogen, carbohydrate and lipid metabolism and fatty acid transport after 1 day of arousal from 6 months aestivation. There were also up-regulation in expressions of genes that were involved in the electron transport system and ATP synthesis, indicating a greater demand for metabolic energy during arousal. Overall, our results signify the importance of sustaining a low rate of waste production and conservation of energy store during the maintenance phase, and the dependence on internal energy store for repair and structural modification during the arousal phase, of aestivation in the liver of P. annectens.</description><subject>Adaptation</subject><subject>Aestivation</subject><subject>Africa</subject><subject>Amino acids</subject><subject>Ammonia</subject><subject>Animals</subject><subject>Argininosuccinate Synthase - metabolism</subject><subject>Arousal</subject><subject>Arousal - physiology</subject><subject>Base Sequence</subject><subject>Betaine</subject><subject>Betaine-Homocysteine S-Methyltransferase - metabolism</subject><subject>Blood coagulation</subject><subject>Carbamoyl phosphate</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Carbon-Nitrogen Ligases - metabolism</subject><subject>Coagulation</subject><subject>Comparative analysis</subject><subject>Complement fixation</subject><subject>Conservation</subject><subject>Copper</subject><subject>Dependence</subject><subject>Dipnoi</subject><subject>DNA, Complementary - genetics</subject><subject>Drought</subject><subject>Electron transport</subject><subject>Energy conservation</subject><subject>Energy metabolism</subject><subject>Energy Metabolism - physiology</subject><subject>Estivation - physiology</subject><subject>Fatty acids</subject><subject>Fish</subject><subject>Fishes - genetics</subject><subject>Fishes - physiology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Library</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Homocysteine</subject><subject>Hybridization</subject><subject>Internal energy</subject><subject>Iron</subject><subject>Ligases</subject><subject>Lipid metabolism</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Maintenance</subject><subject>Metabolic Networks and Pathways - physiology</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Ornithine</subject><subject>Phosphates</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Protopterus aethiopicus</subject><subject>Protopterus amphibius</subject><subject>Protopterus annectens</subject><subject>Protopterus dolloi</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reptiles & amphibians</subject><subject>RNA</subject><subject>Science education</subject><subject>Sequence Analysis, DNA</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Thromboembolism</subject><subject>Thrombosis</subject><subject>Urea</subject><subject>Ureas</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAURU1padK0f1BaQaG0kJnqZll-KQzpLRBI6e1VaKyjGQWPNJHskHxVf7FyxglxSaH4wfbR2lvSlk5RPCd4TlhF3p2FPnrdzrfBwxwTSijlD4p9UjM6ExSzh3e-94onKZ1hXDIpxONij5aS0pLS_eL3B2ctRPCd0y1agQcEl9sIKbngkfOoWwNq3QVEFOz1z8JG12iP2t6vrEvrQ_Q1hi5sO4h9Qtp7aDrw6RBpm0tIoE3w3ToNcg2pcxe6G621y6YREWT01fVwDH3Kq7AxbP6le1o8srpN8Gx8HxQ_P338cfRldnL6-fhocTJrqlJ2M4ObuiKCEWlrZkUjTIVrbKDWSyp5sxS5bmy9rDElVmKQVpSVoFQyrjGnmh0UL3e-2zYkNWadFBGiEgJzjDNxvCNM0GdqG91GxysVtFPXhRBXSsfONS0oXnNuaSW50YyXpqqZLI01zGgBAHTwej_O1i83YJp8HFG3E9PpiHdrtQoXirNK1pRngzejQQznfY5LbVxqoG21hxzqsG5JmeBcZvTVX-j9uxuplc4bcN6GPG8zmKoFJzVmOaw6U_N7qPwY2LgmX0zrcn0ieDsRZKaDy26l-5TU8fdv_8-e_pqyr--wa9Btvjqh7Ycrk6Yg34FNDClFsLchE6yGvrpJQw19pca-yrIXdw_oVnTTSOwPGqIewA</recordid><startdate>20150330</startdate><enddate>20150330</enddate><creator>Hiong, Kum C</creator><creator>Ip, Yuen K</creator><creator>Wong, Wai P</creator><creator>Chew, Shit F</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150330</creationdate><title>Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation</title><author>Hiong, Kum C ; Ip, Yuen K ; Wong, Wai P ; Chew, Shit F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-d0c9716318f93f6c6d7090de9ab284cb68f9df9b9021f80e8f657622834a042a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adaptation</topic><topic>Aestivation</topic><topic>Africa</topic><topic>Amino acids</topic><topic>Ammonia</topic><topic>Animals</topic><topic>Argininosuccinate Synthase - metabolism</topic><topic>Arousal</topic><topic>Arousal - physiology</topic><topic>Base Sequence</topic><topic>Betaine</topic><topic>Betaine-Homocysteine S-Methyltransferase - metabolism</topic><topic>Blood coagulation</topic><topic>Carbamoyl phosphate</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Carbon-Nitrogen Ligases - metabolism</topic><topic>Coagulation</topic><topic>Comparative analysis</topic><topic>Complement fixation</topic><topic>Conservation</topic><topic>Copper</topic><topic>Dependence</topic><topic>Dipnoi</topic><topic>DNA, Complementary - genetics</topic><topic>Drought</topic><topic>Electron transport</topic><topic>Energy conservation</topic><topic>Energy metabolism</topic><topic>Energy Metabolism - physiology</topic><topic>Estivation - physiology</topic><topic>Fatty acids</topic><topic>Fish</topic><topic>Fishes - genetics</topic><topic>Fishes - physiology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Library</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Homocysteine</topic><topic>Hybridization</topic><topic>Internal energy</topic><topic>Iron</topic><topic>Ligases</topic><topic>Lipid metabolism</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Maintenance</topic><topic>Metabolic Networks and Pathways - physiology</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Ornithine</topic><topic>Phosphates</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Protopterus aethiopicus</topic><topic>Protopterus amphibius</topic><topic>Protopterus annectens</topic><topic>Protopterus dolloi</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reptiles & amphibians</topic><topic>RNA</topic><topic>Science education</topic><topic>Sequence Analysis, DNA</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Thromboembolism</topic><topic>Thrombosis</topic><topic>Urea</topic><topic>Ureas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiong, Kum C</creatorcontrib><creatorcontrib>Ip, Yuen K</creatorcontrib><creatorcontrib>Wong, Wai P</creatorcontrib><creatorcontrib>Chew, Shit F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiong, Kum C</au><au>Ip, Yuen K</au><au>Wong, Wai P</au><au>Chew, Shit F</au><au>Foulkes, Nicholas S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-30</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0121224</spage><epage>e0121224</epage><pages>e0121224-e0121224</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the liver of P. annectens after 6 months (the maintenance phase) of aestivation as compared with the freshwater control, or after 1 day of arousal from 6 months aestivation as compared with 6 months of aestivation using suppression subtractive hybridization. During the maintenance phase of aestivation, the mRNA expression of argininosuccinate synthetase 1 and carbamoyl phosphate synthetase III were up-regulated, indicating an increase in the ornithine-urea cycle capacity to detoxify ammonia to urea. There was also an increase in the expression of betaine homocysteine-S-transferase 1 which could reduce and prevent the accumulation of hepatic homocysteine. On the other hand, the down-regulation of superoxide dismutase 1 expression could signify a decrease in ROS production during the maintenance phase of aestivation. In addition, the maintenance phase was marked by decreases in expressions of genes related to blood coagulation, complement fixation and iron and copper metabolism, which could be strategies used to prevent thrombosis and to conserve energy. Unlike the maintenance phase of aestivation, there were increases in expressions of genes related to nitrogen, carbohydrate and lipid metabolism and fatty acid transport after 1 day of arousal from 6 months aestivation. There were also up-regulation in expressions of genes that were involved in the electron transport system and ATP synthesis, indicating a greater demand for metabolic energy during arousal. Overall, our results signify the importance of sustaining a low rate of waste production and conservation of energy store during the maintenance phase, and the dependence on internal energy store for repair and structural modification during the arousal phase, of aestivation in the liver of P. annectens.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25822522</pmid><doi>10.1371/journal.pone.0121224</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-03, Vol.10 (3), p.e0121224-e0121224 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1667660400 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adaptation Aestivation Africa Amino acids Ammonia Animals Argininosuccinate Synthase - metabolism Arousal Arousal - physiology Base Sequence Betaine Betaine-Homocysteine S-Methyltransferase - metabolism Blood coagulation Carbamoyl phosphate Carbohydrate metabolism Carbohydrates Carbon-Nitrogen Ligases - metabolism Coagulation Comparative analysis Complement fixation Conservation Copper Dependence Dipnoi DNA, Complementary - genetics Drought Electron transport Energy conservation Energy metabolism Energy Metabolism - physiology Estivation - physiology Fatty acids Fish Fishes - genetics Fishes - physiology Gene expression Gene Expression Regulation, Enzymologic - physiology Gene Library Gene regulation Genes Homocysteine Hybridization Internal energy Iron Ligases Lipid metabolism Liver Liver - metabolism Maintenance Metabolic Networks and Pathways - physiology Metabolism Molecular Sequence Data Ornithine Phosphates Physiology Proteins Protopterus aethiopicus Protopterus amphibius Protopterus annectens Protopterus dolloi Reactive Oxygen Species - metabolism Real-Time Polymerase Chain Reaction Reptiles & amphibians RNA Science education Sequence Analysis, DNA Superoxide dismutase Superoxide Dismutase - metabolism Thromboembolism Thrombosis Urea Ureas |
| title | Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 months of aestivation in air or 1 day of arousal from 6 months of aestivation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-03T11%3A20%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20gene%20expression%20in%20the%20liver%20of%20the%20African%20lungfish,%20Protopterus%20annectens,%20after%206%20months%20of%20aestivation%20in%20air%20or%201%20day%20of%20arousal%20from%206%20months%20of%20aestivation&rft.jtitle=PloS%20one&rft.au=Hiong,%20Kum%20C&rft.date=2015-03-30&rft.volume=10&rft.issue=3&rft.spage=e0121224&rft.epage=e0121224&rft.pages=e0121224-e0121224&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0121224&rft_dat=%3Cgale_plos_%3EA419036229%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1667660400&rft_id=info:pmid/25822522&rft_galeid=A419036229&rft_doaj_id=oai_doaj_org_article_4944f2784da345d79385dfd3da6eee20&rfr_iscdi=true |