A standardized method for the construction of tracer specific PET and SPECT rat brain templates: validation and implementation of a toolbox
High-resolution anatomical image data in preclinical brain PET and SPECT studies is often not available, and inter-modality spatial normalization to an MRI brain template is frequently performed. However, this procedure can be challenging for tracers where substantial anatomical structures present l...
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| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0122363-e0122363 |
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| creator | Vállez Garcia, David Casteels, Cindy Schwarz, Adam J Dierckx, Rudi A J O Koole, Michel Doorduin, Janine |
| description | High-resolution anatomical image data in preclinical brain PET and SPECT studies is often not available, and inter-modality spatial normalization to an MRI brain template is frequently performed. However, this procedure can be challenging for tracers where substantial anatomical structures present limited tracer uptake. Therefore, we constructed and validated strain- and tracer-specific rat brain templates in Paxinos space to allow intra-modal registration. PET [18F]FDG, [11C]flumazenil, [11C]MeDAS, [11C]PK11195 and [11C]raclopride, and SPECT [99mTc]HMPAO brain scans were acquired from healthy male rats. Tracer-specific templates were constructed by averaging the scans, and by spatial normalization to a widely used MRI-based template. The added value of tracer-specific templates was evaluated by quantification of the residual error between original and realigned voxels after random misalignments of the data set. Additionally, the impact of strain differences, disease uptake patterns (focal and diffuse lesion), and the effect of image and template size on the registration errors were explored. Mean registration errors were 0.70 ± 0.32 mm for [18F]FDG (n = 25), 0.23 ± 0.10mm for [11C]flumazenil (n = 13), 0.88 ± 0.20 mm for [11C]MeDAS (n = 15), 0.64 ± 0.28 mm for [11C]PK11195 (n = 19), 0.34 ± 0.15 mm for [11C]raclopride (n = 6), and 0.40 ± 0.13 mm for [99mTc]HMPAO (n = 15). These values were smallest with tracer-specific templates, when compared to the use of [18F]FDG as reference template (p |
| doi_str_mv | 10.1371/journal.pone.0122363 |
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However, this procedure can be challenging for tracers where substantial anatomical structures present limited tracer uptake. Therefore, we constructed and validated strain- and tracer-specific rat brain templates in Paxinos space to allow intra-modal registration. PET [18F]FDG, [11C]flumazenil, [11C]MeDAS, [11C]PK11195 and [11C]raclopride, and SPECT [99mTc]HMPAO brain scans were acquired from healthy male rats. Tracer-specific templates were constructed by averaging the scans, and by spatial normalization to a widely used MRI-based template. The added value of tracer-specific templates was evaluated by quantification of the residual error between original and realigned voxels after random misalignments of the data set. Additionally, the impact of strain differences, disease uptake patterns (focal and diffuse lesion), and the effect of image and template size on the registration errors were explored. Mean registration errors were 0.70 ± 0.32 mm for [18F]FDG (n = 25), 0.23 ± 0.10mm for [11C]flumazenil (n = 13), 0.88 ± 0.20 mm for [11C]MeDAS (n = 15), 0.64 ± 0.28 mm for [11C]PK11195 (n = 19), 0.34 ± 0.15 mm for [11C]raclopride (n = 6), and 0.40 ± 0.13 mm for [99mTc]HMPAO (n = 15). These values were smallest with tracer-specific templates, when compared to the use of [18F]FDG as reference template (p<0.001). Additionally, registration errors were smallest with strain-specific templates (p<0.05), and when images and templates had the same size (p ≤ 0.001). Moreover, highest registration errors were found for the focal lesion group (p<0.005) and the diffuse lesion group (p = n.s.). In the voxel-based analysis, the reported coordinates of the focal lesion model are consistent with the stereotaxic injection procedure. The use of PET/SPECT strain- and tracer-specific templates allows accurate registration of functional rat brain data, independent of disease specific uptake patterns and with registration error below spatial resolution of the cameras. The templates and the SAMIT package will be freely available for the research community [corrected].</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0122363</identifier><identifier>PMID: 25823005</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Automation ; Brain ; Brain - drug effects ; Brain - pathology ; Brain Mapping - methods ; Cameras ; Construction methods ; Construction standards ; Flumazenil ; Fluorine isotopes ; Fluorodeoxyglucose F18 - administration & dosage ; Image Processing, Computer-Assisted - methods ; Image resolution ; Magnetic Resonance Imaging ; Male ; Medical imaging ; Metabolism ; Methods ; Multiple sclerosis ; Neuroimaging ; Nuclear medicine ; Positron emission ; Positron emission tomography ; Positron-Emission Tomography - methods ; Raclopride ; Raclopride - administration & dosage ; Radioisotopes - administration & dosage ; Radiopharmaceuticals - administration & dosage ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Rodents ; Single photon emission computed tomography ; Software ; Spatial discrimination ; Spatial resolution ; Studies ; Technetium Tc 99m Exametazime - administration & dosage ; Tomography ; Tomography, Emission-Computed, Single-Photon - methods ; Tracers ; Tracers (Biology)</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0122363-e0122363</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Vállez Garcia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Vállez Garcia et al 2015 Vállez Garcia et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d00e9866b6d703a4f628e2c48388a81483cc2a100391db5c8e777de4d73ec0793</citedby><cites>FETCH-LOGICAL-c692t-d00e9866b6d703a4f628e2c48388a81483cc2a100391db5c8e777de4d73ec0793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379068/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379068/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2104,2930,23873,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25823005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Baron, Jean-Claude</contributor><creatorcontrib>Vállez Garcia, David</creatorcontrib><creatorcontrib>Casteels, Cindy</creatorcontrib><creatorcontrib>Schwarz, Adam J</creatorcontrib><creatorcontrib>Dierckx, Rudi A J O</creatorcontrib><creatorcontrib>Koole, Michel</creatorcontrib><creatorcontrib>Doorduin, Janine</creatorcontrib><title>A standardized method for the construction of tracer specific PET and SPECT rat brain templates: validation and implementation of a toolbox</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>High-resolution anatomical image data in preclinical brain PET and SPECT studies is often not available, and inter-modality spatial normalization to an MRI brain template is frequently performed. However, this procedure can be challenging for tracers where substantial anatomical structures present limited tracer uptake. Therefore, we constructed and validated strain- and tracer-specific rat brain templates in Paxinos space to allow intra-modal registration. PET [18F]FDG, [11C]flumazenil, [11C]MeDAS, [11C]PK11195 and [11C]raclopride, and SPECT [99mTc]HMPAO brain scans were acquired from healthy male rats. Tracer-specific templates were constructed by averaging the scans, and by spatial normalization to a widely used MRI-based template. The added value of tracer-specific templates was evaluated by quantification of the residual error between original and realigned voxels after random misalignments of the data set. Additionally, the impact of strain differences, disease uptake patterns (focal and diffuse lesion), and the effect of image and template size on the registration errors were explored. Mean registration errors were 0.70 ± 0.32 mm for [18F]FDG (n = 25), 0.23 ± 0.10mm for [11C]flumazenil (n = 13), 0.88 ± 0.20 mm for [11C]MeDAS (n = 15), 0.64 ± 0.28 mm for [11C]PK11195 (n = 19), 0.34 ± 0.15 mm for [11C]raclopride (n = 6), and 0.40 ± 0.13 mm for [99mTc]HMPAO (n = 15). These values were smallest with tracer-specific templates, when compared to the use of [18F]FDG as reference template (p<0.001). Additionally, registration errors were smallest with strain-specific templates (p<0.05), and when images and templates had the same size (p ≤ 0.001). Moreover, highest registration errors were found for the focal lesion group (p<0.005) and the diffuse lesion group (p = n.s.). In the voxel-based analysis, the reported coordinates of the focal lesion model are consistent with the stereotaxic injection procedure. The use of PET/SPECT strain- and tracer-specific templates allows accurate registration of functional rat brain data, independent of disease specific uptake patterns and with registration error below spatial resolution of the cameras. The templates and the SAMIT package will be freely available for the research community [corrected].</description><subject>Animals</subject><subject>Automation</subject><subject>Brain</subject><subject>Brain - drug effects</subject><subject>Brain - pathology</subject><subject>Brain Mapping - methods</subject><subject>Cameras</subject><subject>Construction methods</subject><subject>Construction standards</subject><subject>Flumazenil</subject><subject>Fluorine isotopes</subject><subject>Fluorodeoxyglucose F18 - administration & dosage</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Image resolution</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Medical imaging</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Multiple sclerosis</subject><subject>Neuroimaging</subject><subject>Nuclear medicine</subject><subject>Positron emission</subject><subject>Positron emission tomography</subject><subject>Positron-Emission Tomography - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vállez Garcia, David</au><au>Casteels, Cindy</au><au>Schwarz, Adam J</au><au>Dierckx, Rudi A J O</au><au>Koole, Michel</au><au>Doorduin, Janine</au><au>Baron, Jean-Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A standardized method for the construction of tracer specific PET and SPECT rat brain templates: validation and implementation of a toolbox</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-30</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0122363</spage><epage>e0122363</epage><pages>e0122363-e0122363</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>High-resolution anatomical image data in preclinical brain PET and SPECT studies is often not available, and inter-modality spatial normalization to an MRI brain template is frequently performed. However, this procedure can be challenging for tracers where substantial anatomical structures present limited tracer uptake. Therefore, we constructed and validated strain- and tracer-specific rat brain templates in Paxinos space to allow intra-modal registration. PET [18F]FDG, [11C]flumazenil, [11C]MeDAS, [11C]PK11195 and [11C]raclopride, and SPECT [99mTc]HMPAO brain scans were acquired from healthy male rats. Tracer-specific templates were constructed by averaging the scans, and by spatial normalization to a widely used MRI-based template. The added value of tracer-specific templates was evaluated by quantification of the residual error between original and realigned voxels after random misalignments of the data set. Additionally, the impact of strain differences, disease uptake patterns (focal and diffuse lesion), and the effect of image and template size on the registration errors were explored. Mean registration errors were 0.70 ± 0.32 mm for [18F]FDG (n = 25), 0.23 ± 0.10mm for [11C]flumazenil (n = 13), 0.88 ± 0.20 mm for [11C]MeDAS (n = 15), 0.64 ± 0.28 mm for [11C]PK11195 (n = 19), 0.34 ± 0.15 mm for [11C]raclopride (n = 6), and 0.40 ± 0.13 mm for [99mTc]HMPAO (n = 15). These values were smallest with tracer-specific templates, when compared to the use of [18F]FDG as reference template (p<0.001). Additionally, registration errors were smallest with strain-specific templates (p<0.05), and when images and templates had the same size (p ≤ 0.001). Moreover, highest registration errors were found for the focal lesion group (p<0.005) and the diffuse lesion group (p = n.s.). In the voxel-based analysis, the reported coordinates of the focal lesion model are consistent with the stereotaxic injection procedure. The use of PET/SPECT strain- and tracer-specific templates allows accurate registration of functional rat brain data, independent of disease specific uptake patterns and with registration error below spatial resolution of the cameras. The templates and the SAMIT package will be freely available for the research community [corrected].</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25823005</pmid><doi>10.1371/journal.pone.0122363</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-03, Vol.10 (3), p.e0122363-e0122363 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1667654530 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Automation Brain Brain - drug effects Brain - pathology Brain Mapping - methods Cameras Construction methods Construction standards Flumazenil Fluorine isotopes Fluorodeoxyglucose F18 - administration & dosage Image Processing, Computer-Assisted - methods Image resolution Magnetic Resonance Imaging Male Medical imaging Metabolism Methods Multiple sclerosis Neuroimaging Nuclear medicine Positron emission Positron emission tomography Positron-Emission Tomography - methods Raclopride Raclopride - administration & dosage Radioisotopes - administration & dosage Radiopharmaceuticals - administration & dosage Rats Rats, Sprague-Dawley Rats, Wistar Rodents Single photon emission computed tomography Software Spatial discrimination Spatial resolution Studies Technetium Tc 99m Exametazime - administration & dosage Tomography Tomography, Emission-Computed, Single-Photon - methods Tracers Tracers (Biology) |
| title | A standardized method for the construction of tracer specific PET and SPECT rat brain templates: validation and implementation of a toolbox |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T04%3A02%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20standardized%20method%20for%20the%20construction%20of%20tracer%20specific%20PET%20and%20SPECT%20rat%20brain%20templates:%20validation%20and%20implementation%20of%20a%20toolbox&rft.jtitle=PloS%20one&rft.au=V%C3%A1llez%20Garcia,%20David&rft.date=2015-03-30&rft.volume=10&rft.issue=3&rft.spage=e0122363&rft.epage=e0122363&rft.pages=e0122363-e0122363&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0122363&rft_dat=%3Cgale_plos_%3EA419036316%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1667654530&rft_id=info:pmid/25823005&rft_galeid=A419036316&rft_doaj_id=oai_doaj_org_article_5548e0bcbfb849a9a5d65fc67bc3a028&rfr_iscdi=true |