Removal of endotoxins from bacteriophage preparations by extraction with organic solvents

Lipopolysaccharide (LPS, endotoxin, pyrogen) constitutes a very troubling contaminant of crude phage lysates produced in Gram-negative bacteria. Toxicity of LPS depends on the strong innate immunity response including the cytokines. Therefore, its removal is important for bacteriophage applications....

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Veröffentlicht in:	PloS one 2015-03, Vol.10 (3), p.e0122672
Hauptverfasser:	Boratynski, Janusz, Szermer-Olearnik, Bozena
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Octanol Bacteria Bacterial infections Bacteriophages - growth & development Bacteriophages - isolation & purification Butanol Chromatography Contaminants Cytokines E coli Endotoxins Escherichia coli Escherichia coli - metabolism Escherichia coli - virology Gram-negative bacteria Immunity Immunology Innate immunity Laboratories Limulus Lipopolysaccharides Liquid-Liquid Extraction Lysates Microorganisms Nanoparticles Octanol Organic Chemicals - chemistry Organic solvents Phages Phenols Proteins Purification Pyrogen Solvents Solvents - chemistry Toxicity Viral Plaque Assay
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