YCZ-18 is a new brassinosteroid biosynthesis inhibitor
Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydr...
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| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0120812-e0120812 |
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| creator | Oh, Keimei Matsumoto, Tadashi Yamagami, Ayumi Ogawa, Atushi Yamada, Kazuhiro Suzuki, Ryuichiro Sawada, Takayuki Fujioka, Shozo Yoshizawa, Yuko Nakano, Takeshi |
| description | Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor, YCZ-18. Quantitative analysis the endogenous levels of BRs in Arabidopsis indicated that YCZ-18 significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of YCZ-18to purified recombinant CYP90D1 indicated that YCZ-18 induced a typical type II binding spectrum with a Kd value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with YCZ-18 treatment of Arabidopsis indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation. |
| doi_str_mv | 10.1371/journal.pone.0120812 |
| format | Article |
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The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor, YCZ-18. Quantitative analysis the endogenous levels of BRs in Arabidopsis indicated that YCZ-18 significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of YCZ-18to purified recombinant CYP90D1 indicated that YCZ-18 induced a typical type II binding spectrum with a Kd value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with YCZ-18 treatment of Arabidopsis indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0120812</identifier><identifier>PMID: 25793645</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antibiotics ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Binding ; Biosynthesis ; Biosynthetic Pathways - drug effects ; Biotechnology ; Brassinosteroids ; Brassinosteroids - biosynthesis ; Cell division ; Cytochrome ; Cytochrome P-450 Enzyme System - metabolism ; Deficient mutant ; Dioxoles - chemistry ; Dioxoles - pharmacology ; Elongation ; Enzymes ; Gene expression ; Gene Expression Regulation, Plant - drug effects ; Gene regulation ; Genes ; Genetic engineering ; Hormones ; Hydroxylase ; Hydroxylation ; Inhibitors ; Laboratories ; Metabolism ; Mutants ; Phenotype ; Phenotypes ; Plant growth ; Plant hormones ; Plant tissues ; Prostheses ; Protein Binding ; Proteins ; Quantitative analysis ; Science ; Seeds ; Senescence ; Steroid hormones ; Steroids ; Steroids (Organic compounds) ; Triazoles - chemistry ; Triazoles - pharmacology</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0120812-e0120812</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Oh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Oh et al 2015 Oh et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-f195a332737eb70329efe33fe8b67de21ceaad04c740fcece6e221eadf9de9cc3</citedby><cites>FETCH-LOGICAL-c758t-f195a332737eb70329efe33fe8b67de21ceaad04c740fcece6e221eadf9de9cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368189/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368189/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25793645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Blazquez, Miguel A</contributor><creatorcontrib>Oh, Keimei</creatorcontrib><creatorcontrib>Matsumoto, Tadashi</creatorcontrib><creatorcontrib>Yamagami, Ayumi</creatorcontrib><creatorcontrib>Ogawa, Atushi</creatorcontrib><creatorcontrib>Yamada, Kazuhiro</creatorcontrib><creatorcontrib>Suzuki, Ryuichiro</creatorcontrib><creatorcontrib>Sawada, Takayuki</creatorcontrib><creatorcontrib>Fujioka, Shozo</creatorcontrib><creatorcontrib>Yoshizawa, Yuko</creatorcontrib><creatorcontrib>Nakano, Takeshi</creatorcontrib><title>YCZ-18 is a new brassinosteroid biosynthesis inhibitor</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. 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Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation.</description><subject>Analysis</subject><subject>Antibiotics</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Binding</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - drug effects</subject><subject>Biotechnology</subject><subject>Brassinosteroids</subject><subject>Brassinosteroids - biosynthesis</subject><subject>Cell division</subject><subject>Cytochrome</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Deficient mutant</subject><subject>Dioxoles - chemistry</subject><subject>Dioxoles - pharmacology</subject><subject>Elongation</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Hormones</subject><subject>Hydroxylase</subject><subject>Hydroxylation</subject><subject>Inhibitors</subject><subject>Laboratories</subject><subject>Metabolism</subject><subject>Mutants</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Plant growth</subject><subject>Plant hormones</subject><subject>Plant tissues</subject><subject>Prostheses</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Quantitative analysis</subject><subject>Science</subject><subject>Seeds</subject><subject>Senescence</subject><subject>Steroid hormones</subject><subject>Steroids</subject><subject>Steroids (Organic compounds)</subject><subject>Triazoles - chemistry</subject><subject>Triazoles - pharmacology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2LEzEUhgdR3HX1H4gWBNGL1snHJJMbYSl-FBYW_AK9CZnkTJsyTWqScXf_vWk7u3RkLyQXCSfPec85yVsUz1E5Q4Sjd2vfB6e62dY7mJUIlzXCD4pTJAieMlySh0fnk-JJjOuyrEjN2OPiBFdcEEar04L9nP-aonpi40RNHFxNmqBitM7HBMFbM2msjzcurSBmxLqVbWzy4WnxqFVdhGfDflZ8__jh2_zz9OLy02J-fjHVvKrTtEWiUoRgTjg0vCRYQAuEtFA3jBvASINSpqSa07LVoIEBxgiUaYUBoTU5K14edLedj3IYOUrEGBUEEYoysTgQxqu13Aa7UeFGemXlPuDDUqqQrO5AUiQUoZpyoxSlpm4MbzHODTFQouEsa70fqvXNBowGl4LqRqLjG2dXcun_SEpYjWqRBd4MAsH_7iEmubFRQ9cpB77f982wyK3XGX31D3r_dAO1VHkA61qf6-qdqDynmNSVIHut2T1UXgY2Vmd_tDbHRwlvRwmZSXCdlqqPUS6-fvl_9vLHmH19xK5AdWkVfdcn610cg_QA6uBjDNDePTIq5c7et68hd_aWg71z2ovjD7pLuvUz-Qs7tfT8</recordid><startdate>20150320</startdate><enddate>20150320</enddate><creator>Oh, Keimei</creator><creator>Matsumoto, Tadashi</creator><creator>Yamagami, Ayumi</creator><creator>Ogawa, Atushi</creator><creator>Yamada, Kazuhiro</creator><creator>Suzuki, Ryuichiro</creator><creator>Sawada, Takayuki</creator><creator>Fujioka, Shozo</creator><creator>Yoshizawa, Yuko</creator><creator>Nakano, Takeshi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150320</creationdate><title>YCZ-18 is a new brassinosteroid biosynthesis inhibitor</title><author>Oh, Keimei ; Matsumoto, Tadashi ; Yamagami, Ayumi ; Ogawa, Atushi ; Yamada, Kazuhiro ; Suzuki, Ryuichiro ; Sawada, Takayuki ; Fujioka, Shozo ; Yoshizawa, Yuko ; Nakano, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-f195a332737eb70329efe33fe8b67de21ceaad04c740fcece6e221eadf9de9cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Antibiotics</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Binding</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - drug effects</topic><topic>Biotechnology</topic><topic>Brassinosteroids</topic><topic>Brassinosteroids - biosynthesis</topic><topic>Cell division</topic><topic>Cytochrome</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Deficient mutant</topic><topic>Dioxoles - chemistry</topic><topic>Dioxoles - pharmacology</topic><topic>Elongation</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Hormones</topic><topic>Hydroxylase</topic><topic>Hydroxylation</topic><topic>Inhibitors</topic><topic>Laboratories</topic><topic>Metabolism</topic><topic>Mutants</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Plant growth</topic><topic>Plant hormones</topic><topic>Plant tissues</topic><topic>Prostheses</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Quantitative analysis</topic><topic>Science</topic><topic>Seeds</topic><topic>Senescence</topic><topic>Steroid hormones</topic><topic>Steroids</topic><topic>Steroids (Organic compounds)</topic><topic>Triazoles - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oh, Keimei</au><au>Matsumoto, Tadashi</au><au>Yamagami, Ayumi</au><au>Ogawa, Atushi</au><au>Yamada, Kazuhiro</au><au>Suzuki, Ryuichiro</au><au>Sawada, Takayuki</au><au>Fujioka, Shozo</au><au>Yoshizawa, Yuko</au><au>Nakano, Takeshi</au><au>Blazquez, Miguel A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>YCZ-18 is a new brassinosteroid biosynthesis inhibitor</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-20</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0120812</spage><epage>e0120812</epage><pages>e0120812-e0120812</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor, YCZ-18. Quantitative analysis the endogenous levels of BRs in Arabidopsis indicated that YCZ-18 significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of YCZ-18to purified recombinant CYP90D1 indicated that YCZ-18 induced a typical type II binding spectrum with a Kd value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with YCZ-18 treatment of Arabidopsis indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25793645</pmid><doi>10.1371/journal.pone.0120812</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-03, Vol.10 (3), p.e0120812-e0120812 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1664931341 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Antibiotics Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - metabolism Arabidopsis thaliana Binding Biosynthesis Biosynthetic Pathways - drug effects Biotechnology Brassinosteroids Brassinosteroids - biosynthesis Cell division Cytochrome Cytochrome P-450 Enzyme System - metabolism Deficient mutant Dioxoles - chemistry Dioxoles - pharmacology Elongation Enzymes Gene expression Gene Expression Regulation, Plant - drug effects Gene regulation Genes Genetic engineering Hormones Hydroxylase Hydroxylation Inhibitors Laboratories Metabolism Mutants Phenotype Phenotypes Plant growth Plant hormones Plant tissues Prostheses Protein Binding Proteins Quantitative analysis Science Seeds Senescence Steroid hormones Steroids Steroids (Organic compounds) Triazoles - chemistry Triazoles - pharmacology |
| title | YCZ-18 is a new brassinosteroid biosynthesis inhibitor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T18%3A59%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=YCZ-18%20is%20a%20new%20brassinosteroid%20biosynthesis%20inhibitor&rft.jtitle=PloS%20one&rft.au=Oh,%20Keimei&rft.date=2015-03-20&rft.volume=10&rft.issue=3&rft.spage=e0120812&rft.epage=e0120812&rft.pages=e0120812-e0120812&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0120812&rft_dat=%3Cgale_plos_%3EA423859338%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1664931341&rft_id=info:pmid/25793645&rft_galeid=A423859338&rft_doaj_id=oai_doaj_org_article_419a34c47daa44d8bd7f22fe36ea9b76&rfr_iscdi=true |