GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana
The WRKY transcription factors modulate numerous physiological processes, including plant growth, development and responses to various environmental stresses. Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is...
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| description | The WRKY transcription factors modulate numerous physiological processes, including plant growth, development and responses to various environmental stresses. Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is limited. In particular, very few WRKYs have been identified and characterised from an economically important crop, cotton. In this study, we characterised a novel group IIc WRKY gene, GhWRKY68, which is induced by different abiotic stresses and multiple defence-related signalling molecules. The β-glucuronidase activity driven by the GhWRKY68 promoter was enhanced after exposure to drought, salt, abscisic acid (ABA) and H2O2. The overexpression of GhWRKY68 in Nicotiana benthamiana reduced resistance to drought and salt and affected several physiological indices. GhWRKY68 may mediate salt and drought responses by modulating ABA content and enhancing the transcript levels of ABA-responsive genes. GhWRKY68-overexpressing plants exhibited reduced tolerance to oxidative stress after drought and salt stress treatments, which correlated with the accumulation of reactive oxygen species (ROS), reduced enzyme activities, elevated malondialdehyde (MDA) content and altered ROS-related gene expression. These results indicate that GhWRKY68 is a transcription factor that responds to drought and salt stresses by regulating ABA signalling and modulating cellular ROS. |
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Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is limited. In particular, very few WRKYs have been identified and characterised from an economically important crop, cotton. In this study, we characterised a novel group IIc WRKY gene, GhWRKY68, which is induced by different abiotic stresses and multiple defence-related signalling molecules. The β-glucuronidase activity driven by the GhWRKY68 promoter was enhanced after exposure to drought, salt, abscisic acid (ABA) and H2O2. The overexpression of GhWRKY68 in Nicotiana benthamiana reduced resistance to drought and salt and affected several physiological indices. GhWRKY68 may mediate salt and drought responses by modulating ABA content and enhancing the transcript levels of ABA-responsive genes. GhWRKY68-overexpressing plants exhibited reduced tolerance to oxidative stress after drought and salt stress treatments, which correlated with the accumulation of reactive oxygen species (ROS), reduced enzyme activities, elevated malondialdehyde (MDA) content and altered ROS-related gene expression. These results indicate that GhWRKY68 is a transcription factor that responds to drought and salt stresses by regulating ABA signalling and modulating cellular ROS.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0120646</identifier><identifier>PMID: 25793865</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic stress ; Abscisic acid ; Abscisic Acid - metabolism ; Amino Acid Sequence ; Arabidopsis ; Arabidopsis - metabolism ; Biology ; Cotton ; Crop diseases ; Crosstalk ; Drought ; Droughts ; Economic importance ; Environmental stress ; Enzymatic activity ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Genes, Plant ; Genetic engineering ; Glucuronidase - metabolism ; Gossypium - drug effects ; Gossypium - genetics ; Gossypium - metabolism ; Gossypium - physiology ; Gossypium hirsutum ; Hydrogen peroxide ; Laboratories ; Life sciences ; Malondialdehyde ; Molecular Sequence Data ; Nicotiana - drug effects ; Nicotiana - genetics ; Nicotiana - physiology ; Nicotiana benthamiana ; Oxidative stress ; Oxidative Stress - drug effects ; Oxygen ; Phylogeny ; Physiological aspects ; Physiological effects ; Physiology ; Plant growth ; Plant Leaves - drug effects ; Plant Leaves - genetics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Promoter Regions, Genetic - genetics ; Proteins ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Salts ; Sequence Alignment ; Sequence Analysis, Protein ; Signal transduction ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signaling ; Sodium Chloride - pharmacology ; Stress, Physiological - drug effects ; Stress, Physiological - genetics ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic - drug effects ; Transgenic plants</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0120646</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Jia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Jia et al 2015 Jia et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-4bbe9268b581b210d2b6abf9d4776624bd37365856486a2aee9eb14d6755f0ae3</citedby><cites>FETCH-LOGICAL-c692t-4bbe9268b581b210d2b6abf9d4776624bd37365856486a2aee9eb14d6755f0ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368093/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368093/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25793865$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Liu, Ji-Hong</contributor><creatorcontrib>Jia, Haihong</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Liu, Shuchang</creatorcontrib><creatorcontrib>Li, Guilin</creatorcontrib><creatorcontrib>Guo, Xingqi</creatorcontrib><title>GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The WRKY transcription factors modulate numerous physiological processes, including plant growth, development and responses to various environmental stresses. Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is limited. In particular, very few WRKYs have been identified and characterised from an economically important crop, cotton. In this study, we characterised a novel group IIc WRKY gene, GhWRKY68, which is induced by different abiotic stresses and multiple defence-related signalling molecules. The β-glucuronidase activity driven by the GhWRKY68 promoter was enhanced after exposure to drought, salt, abscisic acid (ABA) and H2O2. The overexpression of GhWRKY68 in Nicotiana benthamiana reduced resistance to drought and salt and affected several physiological indices. GhWRKY68 may mediate salt and drought responses by modulating ABA content and enhancing the transcript levels of ABA-responsive genes. GhWRKY68-overexpressing plants exhibited reduced tolerance to oxidative stress after drought and salt stress treatments, which correlated with the accumulation of reactive oxygen species (ROS), reduced enzyme activities, elevated malondialdehyde (MDA) content and altered ROS-related gene expression. These results indicate that GhWRKY68 is a transcription factor that responds to drought and salt stresses by regulating ABA signalling and modulating cellular ROS.</description><subject>Abiotic stress</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis</subject><subject>Arabidopsis - metabolism</subject><subject>Biology</subject><subject>Cotton</subject><subject>Crop diseases</subject><subject>Crosstalk</subject><subject>Drought</subject><subject>Droughts</subject><subject>Economic importance</subject><subject>Environmental stress</subject><subject>Enzymatic activity</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes, Plant</subject><subject>Genetic engineering</subject><subject>Glucuronidase - metabolism</subject><subject>Gossypium - drug effects</subject><subject>Gossypium - genetics</subject><subject>Gossypium - metabolism</subject><subject>Gossypium - physiology</subject><subject>Gossypium hirsutum</subject><subject>Hydrogen peroxide</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>Malondialdehyde</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - drug effects</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>Nicotiana benthamiana</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxygen</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Physiological effects</subject><subject>Physiology</subject><subject>Plant growth</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - genetics</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Salts</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, Protein</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Signaling</subject><subject>Sodium Chloride - pharmacology</subject><subject>Stress, Physiological - drug effects</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transgenic plants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A9EBQfBi12TyMTM3hVK0LhYLrR94Fc4kmZkss8maZET_vdnutOyAgoSQQ_KcNycnb5Y9x2iJSYnfrt3oLQzLrbN6iXCBOOUPsmNck2LBC0QeHsRH2ZMQ1ggxUnH-ODsqWFmnkB1nNxf9t-uP33mVe61GqUNagwkRrNR5dHmAIeZgVa68G7s-5sbm0YMNnbZG5p-MdNGAhbzRNvaw2cVPs0ctDEE_m9aT7Mv7d5_PPywury5W52eXC8nrIi5o0-i64FXDKtwUGKmi4dC0taJlyXlBG0VKwlnFOK04FKB1rRtMFS8ZaxFocpK93OtuBxfE1I8gMOe0JijNRKz2hHKwFltvNuB_CwdG3G443wnw0chBC6QqxMtacsWBaqkAQ8NVy1pCGeYUJ63T6bax2Wgl03s9DDPR-Yk1vejcT0EJr9BtMa8mAe9-jDrEf5Q8UR2kqoxtXRKTGxOkOKMFqVhiWKKWf6HSUHqTvsTq1qT9WcKbWUJiov4VOxhDEKub6_9nr77O2dcHbK-TXfrghjEaZ8McpHtQeheC1-195zASO0PfdUPsDC0mQ6e0F4ddv0-6czD5A6lJ8AA</recordid><startdate>20150320</startdate><enddate>20150320</enddate><creator>Jia, Haihong</creator><creator>Wang, Chen</creator><creator>Wang, Fang</creator><creator>Liu, Shuchang</creator><creator>Li, Guilin</creator><creator>Guo, Xingqi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150320</creationdate><title>GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana</title><author>Jia, Haihong ; Wang, Chen ; Wang, Fang ; Liu, Shuchang ; Li, Guilin ; Guo, Xingqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-4bbe9268b581b210d2b6abf9d4776624bd37365856486a2aee9eb14d6755f0ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abiotic stress</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis</topic><topic>Arabidopsis - metabolism</topic><topic>Biology</topic><topic>Cotton</topic><topic>Crop diseases</topic><topic>Crosstalk</topic><topic>Drought</topic><topic>Droughts</topic><topic>Economic importance</topic><topic>Environmental stress</topic><topic>Enzymatic activity</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes, Plant</topic><topic>Genetic engineering</topic><topic>Glucuronidase - metabolism</topic><topic>Gossypium - drug effects</topic><topic>Gossypium - genetics</topic><topic>Gossypium - metabolism</topic><topic>Gossypium - physiology</topic><topic>Gossypium hirsutum</topic><topic>Hydrogen peroxide</topic><topic>Laboratories</topic><topic>Life sciences</topic><topic>Malondialdehyde</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - drug effects</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>Nicotiana benthamiana</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxygen</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Physiological effects</topic><topic>Physiology</topic><topic>Plant growth</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - genetics</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Salts</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, Protein</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Signaling</topic><topic>Sodium Chloride - pharmacology</topic><topic>Stress, Physiological - drug effects</topic><topic>Stress, Physiological - 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Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is limited. In particular, very few WRKYs have been identified and characterised from an economically important crop, cotton. In this study, we characterised a novel group IIc WRKY gene, GhWRKY68, which is induced by different abiotic stresses and multiple defence-related signalling molecules. The β-glucuronidase activity driven by the GhWRKY68 promoter was enhanced after exposure to drought, salt, abscisic acid (ABA) and H2O2. The overexpression of GhWRKY68 in Nicotiana benthamiana reduced resistance to drought and salt and affected several physiological indices. GhWRKY68 may mediate salt and drought responses by modulating ABA content and enhancing the transcript levels of ABA-responsive genes. GhWRKY68-overexpressing plants exhibited reduced tolerance to oxidative stress after drought and salt stress treatments, which correlated with the accumulation of reactive oxygen species (ROS), reduced enzyme activities, elevated malondialdehyde (MDA) content and altered ROS-related gene expression. These results indicate that GhWRKY68 is a transcription factor that responds to drought and salt stresses by regulating ABA signalling and modulating cellular ROS.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25793865</pmid><doi>10.1371/journal.pone.0120646</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; PMC (PubMed Central); DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) Journals Open Acc |
| subjects | Abiotic stress Abscisic acid Abscisic Acid - metabolism Amino Acid Sequence Arabidopsis Arabidopsis - metabolism Biology Cotton Crop diseases Crosstalk Drought Droughts Economic importance Environmental stress Enzymatic activity Gene expression Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Genes, Plant Genetic engineering Glucuronidase - metabolism Gossypium - drug effects Gossypium - genetics Gossypium - metabolism Gossypium - physiology Gossypium hirsutum Hydrogen peroxide Laboratories Life sciences Malondialdehyde Molecular Sequence Data Nicotiana - drug effects Nicotiana - genetics Nicotiana - physiology Nicotiana benthamiana Oxidative stress Oxidative Stress - drug effects Oxygen Phylogeny Physiological aspects Physiological effects Physiology Plant growth Plant Leaves - drug effects Plant Leaves - genetics Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Promoter Regions, Genetic - genetics Proteins Reactive oxygen species Reactive Oxygen Species - metabolism Salts Sequence Alignment Sequence Analysis, Protein Signal transduction Signal Transduction - drug effects Signal Transduction - genetics Signaling Sodium Chloride - pharmacology Stress, Physiological - drug effects Stress, Physiological - genetics Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic - drug effects Transgenic plants |
| title | GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T03%3A40%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GhWRKY68%20reduces%20resistance%20to%20salt%20and%20drought%20in%20transgenic%20Nicotiana%20benthamiana&rft.jtitle=PloS%20one&rft.au=Jia,%20Haihong&rft.date=2015-03-20&rft.volume=10&rft.issue=3&rft.spage=e0120646&rft.pages=e0120646-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0120646&rft_dat=%3Cgale_plos_%3EA423859335%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1664930493&rft_id=info:pmid/25793865&rft_galeid=A423859335&rft_doaj_id=oai_doaj_org_article_0d80679c6d6a4ecda1ab6df5f3451641&rfr_iscdi=true |