Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge
Here, we report the immunogenicity of a sublingually delivered, trivalent human papillomavirus (HPV) DNA vaccine encapsidated in a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus nanovector. The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encodi...
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| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0119408-e0119408 |
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| creator | Lee, Hee-Jung Cho, Hansam Kim, Mi-Gyeong Heo, Yoon-Ki Cho, Yeondong Gwon, Yong-Dae Park, Ki Hoon Jin, Hyerim Kim, Jinyoung Oh, Yu-Kyoung Kim, Young Bong |
| description | Here, we report the immunogenicity of a sublingually delivered, trivalent human papillomavirus (HPV) DNA vaccine encapsidated in a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus nanovector. The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encoding HPV16L1, -18L1 and -58L1 (AcHERV-triHPV), was constructed and sublingually administered to mice without adjuvant. Following sublingual (SL) administration, AcHERV-triHPV was absorbed and distributed throughout the body. At 15 minutes and 1 day post-dose, the distribution of AcHERV-triHPV to the lung was higher than that to other tissues. At 30 days post-dose, the levels of AcHERV-triHPV had diminished throughout the body. Six weeks after the first of three doses, 1×10(8) copies of SL AcHERV-triHPV induced HPV type-specific serum IgG and neutralizing antibodies to a degree comparable to that of IM immunization with 1×10(9) copies. AcHERV-triHPV induced HPV type-specific vaginal IgA titers in a dose-dependent manner. SL immunization with 1×10(10) copies of AcHERV-triHPV induced Th1 and Th2 cellular responses comparable to IM immunization with 1×10(9) copies. Molecular imaging revealed that SL AcHERV-triHPV in mice provided complete protection against vaginal challenge with HPV16, HPV18, and HPV58 pseudoviruses. These results support the potential of SL immunization using multivalent DNA vaccine in baculovirus nanovector for induction of mucosal, systemic, and cellular immune responses. |
| doi_str_mv | 10.1371/journal.pone.0119408 |
| format | Article |
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The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encoding HPV16L1, -18L1 and -58L1 (AcHERV-triHPV), was constructed and sublingually administered to mice without adjuvant. Following sublingual (SL) administration, AcHERV-triHPV was absorbed and distributed throughout the body. At 15 minutes and 1 day post-dose, the distribution of AcHERV-triHPV to the lung was higher than that to other tissues. At 30 days post-dose, the levels of AcHERV-triHPV had diminished throughout the body. Six weeks after the first of three doses, 1×10(8) copies of SL AcHERV-triHPV induced HPV type-specific serum IgG and neutralizing antibodies to a degree comparable to that of IM immunization with 1×10(9) copies. AcHERV-triHPV induced HPV type-specific vaginal IgA titers in a dose-dependent manner. SL immunization with 1×10(10) copies of AcHERV-triHPV induced Th1 and Th2 cellular responses comparable to IM immunization with 1×10(9) copies. Molecular imaging revealed that SL AcHERV-triHPV in mice provided complete protection against vaginal challenge with HPV16, HPV18, and HPV58 pseudoviruses. These results support the potential of SL immunization using multivalent DNA vaccine in baculovirus nanovector for induction of mucosal, systemic, and cellular immune responses.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119408</identifier><identifier>PMID: 25789464</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Sublingual ; Animal tissues ; Animals ; Antibodies ; Antigens ; Baculoviridae - genetics ; Baculovirus ; Capsid Proteins - administration & dosage ; Capsid Proteins - genetics ; Capsid Proteins - immunology ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; Drug delivery systems ; Female ; Genetic Vectors ; Helper cells ; Human papillomavirus ; Human papillomavirus 16 - genetics ; Human papillomavirus 16 - immunology ; Human papillomavirus 18 - genetics ; Human papillomavirus 18 - immunology ; Humans ; Immune response ; Immune response (cell-mediated) ; Immunity, Cellular - drug effects ; Immunization ; Immunogenicity ; Immunoglobulin A ; Immunoglobulin G ; Laboratory animals ; Lungs ; Lymphocytes T ; Mice ; Mucosal immunity ; Oral administration ; Papillomavirus ; Papillomavirus Infections - immunology ; Papillomavirus Infections - prevention & control ; Papillomavirus Vaccines - administration & dosage ; Papillomavirus Vaccines - immunology ; Pharmaceutical sciences ; Pharmacy ; Proteins ; Rodents ; Studies ; Vaccines ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Vagina ; Vagina - drug effects ; Vagina - immunology ; Viruses</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119408-e0119408</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Lee et al 2015 Lee et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-c8eb417b62684b78068bcf9fa4a1bd23faa69992e485688f47afa51d0dc5b46c3</citedby><cites>FETCH-LOGICAL-c692t-c8eb417b62684b78068bcf9fa4a1bd23faa69992e485688f47afa51d0dc5b46c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366369/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366369/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25789464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hee-Jung</creatorcontrib><creatorcontrib>Cho, Hansam</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Heo, Yoon-Ki</creatorcontrib><creatorcontrib>Cho, Yeondong</creatorcontrib><creatorcontrib>Gwon, Yong-Dae</creatorcontrib><creatorcontrib>Park, Ki Hoon</creatorcontrib><creatorcontrib>Jin, Hyerim</creatorcontrib><creatorcontrib>Kim, Jinyoung</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><creatorcontrib>Kim, Young Bong</creatorcontrib><title>Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Here, we report the immunogenicity of a sublingually delivered, trivalent human papillomavirus (HPV) DNA vaccine encapsidated in a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus nanovector. The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encoding HPV16L1, -18L1 and -58L1 (AcHERV-triHPV), was constructed and sublingually administered to mice without adjuvant. Following sublingual (SL) administration, AcHERV-triHPV was absorbed and distributed throughout the body. At 15 minutes and 1 day post-dose, the distribution of AcHERV-triHPV to the lung was higher than that to other tissues. At 30 days post-dose, the levels of AcHERV-triHPV had diminished throughout the body. Six weeks after the first of three doses, 1×10(8) copies of SL AcHERV-triHPV induced HPV type-specific serum IgG and neutralizing antibodies to a degree comparable to that of IM immunization with 1×10(9) copies. AcHERV-triHPV induced HPV type-specific vaginal IgA titers in a dose-dependent manner. SL immunization with 1×10(10) copies of AcHERV-triHPV induced Th1 and Th2 cellular responses comparable to IM immunization with 1×10(9) copies. Molecular imaging revealed that SL AcHERV-triHPV in mice provided complete protection against vaginal challenge with HPV16, HPV18, and HPV58 pseudoviruses. These results support the potential of SL immunization using multivalent DNA vaccine in baculovirus nanovector for induction of mucosal, systemic, and cellular immune responses.</description><subject>Administration, Sublingual</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Baculoviridae - genetics</subject><subject>Baculovirus</subject><subject>Capsid Proteins - administration & dosage</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - immunology</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA vaccines</subject><subject>Drug delivery systems</subject><subject>Female</subject><subject>Genetic Vectors</subject><subject>Helper cells</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 16 - genetics</subject><subject>Human papillomavirus 16 - immunology</subject><subject>Human papillomavirus 18 - genetics</subject><subject>Human papillomavirus 18 - immunology</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune response (cell-mediated)</subject><subject>Immunity, Cellular - drug effects</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin G</subject><subject>Laboratory animals</subject><subject>Lungs</subject><subject>Lymphocytes T</subject><subject>Mice</subject><subject>Mucosal immunity</subject><subject>Oral administration</subject><subject>Papillomavirus</subject><subject>Papillomavirus Infections - immunology</subject><subject>Papillomavirus Infections - prevention & control</subject><subject>Papillomavirus Vaccines - administration & dosage</subject><subject>Papillomavirus Vaccines - immunology</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacy</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Studies</subject><subject>Vaccines</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Vagina</subject><subject>Vagina - drug effects</subject><subject>Vagina - immunology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-K1DAUxoso7rr6BqIFQfRixiRN0-RGWNZ_C4sLrnobTtOkkyFNxqQd1Cfwsc3szC4zshdSStvk930n_ZJTFE8xmuOqwW-WYYoe3HwVvJ4jjAVF_F5xjEVFZoyg6v7e-1HxKKUlQnXFGXtYHJG64YIyelz8uZpaZ30_gSvtMEze_obRBl8GU47RrsFpP5aLaQBfrmBlnQsDrG2cUvnu82m5BqWs16X1ZQtqcmE75cGHtVZjiKXJ9yqGMX9tbKEH69OYhb3Nqy_VAlwu0evHxQMDLuknu-dJ8e3D-69nn2YXlx_Pz04vZooJMs4U1y3FTcsI47RtOGK8VUYYoIDbjlQGgAkhiKa8Zpwb2oCBGneoU3VLmapOiudb35ULSe5CTBIzRhtOGsEycb4lugBLuYp2gPhLBrDyeiDEXkIcrXJatpxVhDQ5zNrQDogQGDUYCcxxg6ipstfbXbWpHXSncpgR3IHp4Yy3C9mHtaQVYxUT2eDVziCGH5NOoxxsUto58DpM1-uuMaEE8Yy--Ae9--92VJ-3VlpvQq6rNqbylJKK15yLTdn5HVS-Oj1YlU-csXn8QPD6QJCZUf8ce5hSkudXX_6fvfx-yL7cYxca3LhIwU2bw5QOQboFVQwpRW1uQ8ZIbhrmJg25aRi5a5gse7a_Qbeimw6p_gILHhMv</recordid><startdate>20150319</startdate><enddate>20150319</enddate><creator>Lee, Hee-Jung</creator><creator>Cho, Hansam</creator><creator>Kim, Mi-Gyeong</creator><creator>Heo, Yoon-Ki</creator><creator>Cho, Yeondong</creator><creator>Gwon, Yong-Dae</creator><creator>Park, Ki Hoon</creator><creator>Jin, Hyerim</creator><creator>Kim, Jinyoung</creator><creator>Oh, Yu-Kyoung</creator><creator>Kim, Young Bong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>COVID</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150319</creationdate><title>Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge</title><author>Lee, Hee-Jung ; Cho, Hansam ; Kim, Mi-Gyeong ; Heo, Yoon-Ki ; Cho, Yeondong ; Gwon, Yong-Dae ; Park, Ki Hoon ; Jin, Hyerim ; Kim, Jinyoung ; Oh, Yu-Kyoung ; Kim, Young Bong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-c8eb417b62684b78068bcf9fa4a1bd23faa69992e485688f47afa51d0dc5b46c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Administration, Sublingual</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Baculoviridae - genetics</topic><topic>Baculovirus</topic><topic>Capsid Proteins - administration & dosage</topic><topic>Capsid Proteins - genetics</topic><topic>Capsid Proteins - immunology</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA vaccines</topic><topic>Drug delivery systems</topic><topic>Female</topic><topic>Genetic Vectors</topic><topic>Helper cells</topic><topic>Human papillomavirus</topic><topic>Human papillomavirus 16 - genetics</topic><topic>Human papillomavirus 16 - immunology</topic><topic>Human papillomavirus 18 - genetics</topic><topic>Human papillomavirus 18 - immunology</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune response (cell-mediated)</topic><topic>Immunity, Cellular - drug effects</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin G</topic><topic>Laboratory animals</topic><topic>Lungs</topic><topic>Lymphocytes T</topic><topic>Mice</topic><topic>Mucosal immunity</topic><topic>Oral administration</topic><topic>Papillomavirus</topic><topic>Papillomavirus Infections - immunology</topic><topic>Papillomavirus Infections - prevention & control</topic><topic>Papillomavirus Vaccines - administration & dosage</topic><topic>Papillomavirus Vaccines - immunology</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacy</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Studies</topic><topic>Vaccines</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Vagina</topic><topic>Vagina - drug effects</topic><topic>Vagina - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hee-Jung</creatorcontrib><creatorcontrib>Cho, Hansam</creatorcontrib><creatorcontrib>Kim, Mi-Gyeong</creatorcontrib><creatorcontrib>Heo, Yoon-Ki</creatorcontrib><creatorcontrib>Cho, Yeondong</creatorcontrib><creatorcontrib>Gwon, Yong-Dae</creatorcontrib><creatorcontrib>Park, Ki Hoon</creatorcontrib><creatorcontrib>Jin, Hyerim</creatorcontrib><creatorcontrib>Kim, Jinyoung</creatorcontrib><creatorcontrib>Oh, Yu-Kyoung</creatorcontrib><creatorcontrib>Kim, Young Bong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hee-Jung</au><au>Cho, Hansam</au><au>Kim, Mi-Gyeong</au><au>Heo, Yoon-Ki</au><au>Cho, Yeondong</au><au>Gwon, Yong-Dae</au><au>Park, Ki Hoon</au><au>Jin, Hyerim</au><au>Kim, Jinyoung</au><au>Oh, Yu-Kyoung</au><au>Kim, Young Bong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-19</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0119408</spage><epage>e0119408</epage><pages>e0119408-e0119408</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Here, we report the immunogenicity of a sublingually delivered, trivalent human papillomavirus (HPV) DNA vaccine encapsidated in a human endogenous retrovirus (HERV) envelope-coated, nonreplicable, baculovirus nanovector. The HERV envelope-coated, nonreplicable, baculovirus-based DNA vaccine, encoding HPV16L1, -18L1 and -58L1 (AcHERV-triHPV), was constructed and sublingually administered to mice without adjuvant. Following sublingual (SL) administration, AcHERV-triHPV was absorbed and distributed throughout the body. At 15 minutes and 1 day post-dose, the distribution of AcHERV-triHPV to the lung was higher than that to other tissues. At 30 days post-dose, the levels of AcHERV-triHPV had diminished throughout the body. Six weeks after the first of three doses, 1×10(8) copies of SL AcHERV-triHPV induced HPV type-specific serum IgG and neutralizing antibodies to a degree comparable to that of IM immunization with 1×10(9) copies. AcHERV-triHPV induced HPV type-specific vaginal IgA titers in a dose-dependent manner. SL immunization with 1×10(10) copies of AcHERV-triHPV induced Th1 and Th2 cellular responses comparable to IM immunization with 1×10(9) copies. Molecular imaging revealed that SL AcHERV-triHPV in mice provided complete protection against vaginal challenge with HPV16, HPV18, and HPV58 pseudoviruses. These results support the potential of SL immunization using multivalent DNA vaccine in baculovirus nanovector for induction of mucosal, systemic, and cellular immune responses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25789464</pmid><doi>10.1371/journal.pone.0119408</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-03, Vol.10 (3), p.e0119408-e0119408 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1664782796 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Administration, Sublingual Animal tissues Animals Antibodies Antigens Baculoviridae - genetics Baculovirus Capsid Proteins - administration & dosage Capsid Proteins - genetics Capsid Proteins - immunology Cytotoxicity Deoxyribonucleic acid DNA DNA vaccines Drug delivery systems Female Genetic Vectors Helper cells Human papillomavirus Human papillomavirus 16 - genetics Human papillomavirus 16 - immunology Human papillomavirus 18 - genetics Human papillomavirus 18 - immunology Humans Immune response Immune response (cell-mediated) Immunity, Cellular - drug effects Immunization Immunogenicity Immunoglobulin A Immunoglobulin G Laboratory animals Lungs Lymphocytes T Mice Mucosal immunity Oral administration Papillomavirus Papillomavirus Infections - immunology Papillomavirus Infections - prevention & control Papillomavirus Vaccines - administration & dosage Papillomavirus Vaccines - immunology Pharmaceutical sciences Pharmacy Proteins Rodents Studies Vaccines Vaccines, DNA - administration & dosage Vaccines, DNA - immunology Vagina Vagina - drug effects Vagina - immunology Viruses |
| title | Sublingual immunization of trivalent human papillomavirus DNA vaccine in baculovirus nanovector for protection against vaginal challenge |
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