In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes
Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technic...
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| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0119735-e0119735 |
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| creator | Uppangala, Shubhashree Dhiman, Shilly Salian, Sujit Raj Singh, Vikram Jeet Kalthur, Guruprasad Adiga, Satish Kumar |
| description | Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome.
The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes.
Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI.
A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P |
| doi_str_mv | 10.1371/journal.pone.0119735 |
| format | Article |
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The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes.
Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI.
A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001).
The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119735</identifier><identifier>PMID: 25786120</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Birth defects ; Children ; Congenital defects ; Deoxyribonucleic acid ; DNA ; Embryology ; Female ; Fertilization ; Gynecology ; Histones - metabolism ; In Vitro Techniques - methods ; Integrity ; Mammals ; Mice ; Mitochondria ; Mitochondria - physiology ; Obstetrics ; Oocytes ; Oocytes - metabolism ; Oocytes - physiology ; Oxygen ; Phosphorylation ; Pregnancy ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Risk factors ; Sperm ; Sperm Injections, Intracytoplasmic - adverse effects ; Statistics, Nonparametric</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119735-e0119735</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Uppangala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Uppangala et al 2015 Uppangala et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ada6c3e8c6c6c5279c604cfd5b27950882a29be9c22f0834d23c8aad1775dd463</citedby><cites>FETCH-LOGICAL-c692t-ada6c3e8c6c6c5279c604cfd5b27950882a29be9c22f0834d23c8aad1775dd463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364773/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364773/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23870,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25786120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nikitovic-Tzanakaki, Dragana</contributor><creatorcontrib>Uppangala, Shubhashree</creatorcontrib><creatorcontrib>Dhiman, Shilly</creatorcontrib><creatorcontrib>Salian, Sujit Raj</creatorcontrib><creatorcontrib>Singh, Vikram Jeet</creatorcontrib><creatorcontrib>Kalthur, Guruprasad</creatorcontrib><creatorcontrib>Adiga, Satish Kumar</creatorcontrib><title>In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome.
The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes.
Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI.
A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001).
The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.</description><subject>Animals</subject><subject>Birth defects</subject><subject>Children</subject><subject>Congenital defects</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Embryology</subject><subject>Female</subject><subject>Fertilization</subject><subject>Gynecology</subject><subject>Histones - metabolism</subject><subject>In Vitro Techniques - methods</subject><subject>Integrity</subject><subject>Mammals</subject><subject>Mice</subject><subject>Mitochondria</subject><subject>Mitochondria - physiology</subject><subject>Obstetrics</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Oocytes - physiology</subject><subject>Oxygen</subject><subject>Phosphorylation</subject><subject>Pregnancy</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uppangala, Shubhashree</au><au>Dhiman, Shilly</au><au>Salian, Sujit Raj</au><au>Singh, Vikram Jeet</au><au>Kalthur, Guruprasad</au><au>Adiga, Satish Kumar</au><au>Nikitovic-Tzanakaki, Dragana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-18</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0119735</spage><epage>e0119735</epage><pages>e0119735-e0119735</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Concerns regarding the safety of ICSI have been intensified recently due to increased risk of birth defects in ICSI born children. Although fertilization rate is significantly higher in ICSI cycles, studies have failed to demonstrate the benefits of ICSI in improving the pregnancy rate. Poor technical skill, and suboptimal in vitro conditions may account for the ICSI results however, there is no report on the effects of oocyte manipulations on the ICSI outcome.
The present study elucidates the influence of mock ICSI on the functional and genetic integrity of the mouse oocytes.
Reactive Oxygen Species (ROS) level, mitochondrial status, and phosphorylation of H2AX were assessed in the in vivo matured and IVM oocytes subjected to mock ICSI.
A significant increase in ROS level was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P<0.05-0.001) whereas unique mitochondrial distribution pattern was found only in IVM oocytes (P<0.01-0.001). Importantly, differential H2AX phosphorylation was observed in both in vivo matured and IVM oocytes subjected to mock ICSI (P <0.001).
The data from this study suggests that mock ICSI can alter genetic and functional integrity in oocytes and IVM oocytes are more vulnerable to mock ICSI induced changes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25786120</pmid><doi>10.1371/journal.pone.0119735</doi><tpages>e0119735</tpages><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Animals Birth defects Children Congenital defects Deoxyribonucleic acid DNA Embryology Female Fertilization Gynecology Histones - metabolism In Vitro Techniques - methods Integrity Mammals Mice Mitochondria Mitochondria - physiology Obstetrics Oocytes Oocytes - metabolism Oocytes - physiology Oxygen Phosphorylation Pregnancy Reactive oxygen species Reactive Oxygen Species - metabolism Risk factors Sperm Sperm Injections, Intracytoplasmic - adverse effects Statistics, Nonparametric |
| title | In vitro matured oocytes are more susceptible than in vivo matured oocytes to mock ICSI induced functional and genetic changes |
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