Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner
Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly...
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description | Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties. |
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The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117203</identifier><identifier>PMID: 25756283</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adjuvants, Immunologic - administration & dosage ; Adjuvants, Immunologic - chemistry ; Adjuvants, Pharmaceutic - administration & dosage ; Adjuvants, Pharmaceutic - chemistry ; Analysis ; Animals ; Antigens ; Biocompatibility ; Bioengineering ; Chemical properties ; Female ; Formulations ; Glycolic acid ; Health aspects ; Hepatitis A Antibodies - metabolism ; Immune response ; Immune system ; Immunogenicity ; In vivo methods and tests ; Influenza ; Influenza A ; Influenza Vaccines - administration & dosage ; Influenza Vaccines - chemistry ; Influenza Vaccines - immunology ; Interferon ; Lactic Acid - chemistry ; Mice ; Mice, Inbred BALB C ; Nanoparticles ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Nanotechnology ; Particle size ; Pathogens ; Peptides ; Pharmaceutical sciences ; Physicochemical properties ; Polyesters - chemistry ; Polyethylene glycol ; Polyglycolic Acid - chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer ; Polylactide-co-glycolide ; Properties (attributes) ; Proteins ; Silica ; Silicon dioxide ; Silicon Dioxide - chemistry ; Splenocytes ; Studies ; Surface Properties ; Vaccines ; Viral Matrix Proteins - immunology ; Viruses</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0117203-e0117203</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Seth et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Seth et al 2015 Seth et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</citedby><cites>FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355484/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355484/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25756283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lu, Shan</contributor><creatorcontrib>Seth, Arjun</creatorcontrib><creatorcontrib>Ritchie, Fiona K</creatorcontrib><creatorcontrib>Wibowo, Nani</creatorcontrib><creatorcontrib>Lua, Linda H L</creatorcontrib><creatorcontrib>Middelberg, Anton P J</creatorcontrib><title>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</description><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Adjuvants, Immunologic - chemistry</subject><subject>Adjuvants, Pharmaceutic - administration & dosage</subject><subject>Adjuvants, Pharmaceutic - chemistry</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biocompatibility</subject><subject>Bioengineering</subject><subject>Chemical properties</subject><subject>Female</subject><subject>Formulations</subject><subject>Glycolic acid</subject><subject>Health aspects</subject><subject>Hepatitis A Antibodies - metabolism</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunogenicity</subject><subject>In vivo methods and tests</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza Vaccines - 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immunology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7jr6D0QLguhFx-az7Y2wLH4MLC74BV6FNDmdydBJukk76L833eksU9kL6UUP6XPek_f0nCR5jvIlIgV6t3WDt7Jdds7CMkeowDl5kJyjiuCMx_jhSXyWPAlhm-eMlJw_Ts4wKxjHJTlPfn1xNlPSewM-tdK6TvreqBZCKvV22Evbpzunh1b6tPOuB2PTvVTKWEhjKNMjn2nowGoYeWkt-KfJo0a2AZ5N70Xy4-OH75efs6vrT6vLi6tM8Qr3WcUrUtacQklyjJiONpqCAWF5jVW8cN3kGqumUHnZEK1rCZxwSrnSSDW6aMgieXnQ7VoXxNSVIBDnqCoLXLFIrA6EdnIrOm920v8RThpxe-D8WkwmhC4oVERDTRWhlFZVzaKE4sBkiTQaq72fqg31DrSKfr1sZ6LzL9ZsxNrtBSWM0ZJGgTeTgHc3A4Re7ExQ0LbSghtu742jw5zhiL76B73f3UStZTRgbONiXTWKiguKOSt5GedgkSzvoeKjYWdUHKHGxPNZwttZQmR6-N2v5RCCWH37-v_s9c85-_qE3YBs-01w7dAbZ8McpAdQeReCh-auySgX4wYcuyHGDRDTBsS0F6c_6C7pOPLkLyiDAXE</recordid><startdate>20150310</startdate><enddate>20150310</enddate><creator>Seth, Arjun</creator><creator>Ritchie, Fiona K</creator><creator>Wibowo, Nani</creator><creator>Lua, Linda H L</creator><creator>Middelberg, Anton P J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150310</creationdate><title>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</title><author>Seth, Arjun ; Ritchie, Fiona K ; Wibowo, Nani ; Lua, Linda H L ; Middelberg, Anton P J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adjuvants, Immunologic - 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administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Nanotechnology</topic><topic>Particle size</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>Pharmaceutical sciences</topic><topic>Physicochemical properties</topic><topic>Polyesters - chemistry</topic><topic>Polyethylene glycol</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer</topic><topic>Polylactide-co-glycolide</topic><topic>Properties (attributes)</topic><topic>Proteins</topic><topic>Silica</topic><topic>Silicon dioxide</topic><topic>Silicon Dioxide - chemistry</topic><topic>Splenocytes</topic><topic>Studies</topic><topic>Surface Properties</topic><topic>Vaccines</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seth, Arjun</creatorcontrib><creatorcontrib>Ritchie, Fiona K</creatorcontrib><creatorcontrib>Wibowo, Nani</creatorcontrib><creatorcontrib>Lua, Linda H L</creatorcontrib><creatorcontrib>Middelberg, Anton P J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25756283</pmid><doi>10.1371/journal.pone.0117203</doi><oa>free_for_read</oa></addata></record> |
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subjects | Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - chemistry Adjuvants, Pharmaceutic - administration & dosage Adjuvants, Pharmaceutic - chemistry Analysis Animals Antigens Biocompatibility Bioengineering Chemical properties Female Formulations Glycolic acid Health aspects Hepatitis A Antibodies - metabolism Immune response Immune system Immunogenicity In vivo methods and tests Influenza Influenza A Influenza Vaccines - administration & dosage Influenza Vaccines - chemistry Influenza Vaccines - immunology Interferon Lactic Acid - chemistry Mice Mice, Inbred BALB C Nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Nanotechnology Particle size Pathogens Peptides Pharmaceutical sciences Physicochemical properties Polyesters - chemistry Polyethylene glycol Polyglycolic Acid - chemistry Polylactic Acid-Polyglycolic Acid Copolymer Polylactide-co-glycolide Properties (attributes) Proteins Silica Silicon dioxide Silicon Dioxide - chemistry Splenocytes Studies Surface Properties Vaccines Viral Matrix Proteins - immunology Viruses |
title | Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T16%3A25%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-carrier%20nanoparticles%20adjuvant%20modular%20protein%20vaccine%20in%20a%20particle-dependent%20manner&rft.jtitle=PloS%20one&rft.au=Seth,%20Arjun&rft.date=2015-03-10&rft.volume=10&rft.issue=3&rft.spage=e0117203&rft.epage=e0117203&rft.pages=e0117203-e0117203&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117203&rft_dat=%3Cgale_plos_%3EA426586819%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1661987295&rft_id=info:pmid/25756283&rft_galeid=A426586819&rft_doaj_id=oai_doaj_org_article_d74e93deb4c344499b5729c6e5a81d1f&rfr_iscdi=true |