Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner

Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2015-03, Vol.10 (3), p.e0117203-e0117203
Hauptverfasser: Seth, Arjun, Ritchie, Fiona K, Wibowo, Nani, Lua, Linda H L, Middelberg, Anton P J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page e0117203
container_issue 3
container_start_page e0117203
container_title PloS one
container_volume 10
creator Seth, Arjun
Ritchie, Fiona K
Wibowo, Nani
Lua, Linda H L
Middelberg, Anton P J
description Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.
doi_str_mv 10.1371/journal.pone.0117203
format Article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1661987295</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A426586819</galeid><doaj_id>oai_doaj_org_article_d74e93deb4c344499b5729c6e5a81d1f</doaj_id><sourcerecordid>A426586819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7jr6D0QLguhFx-az7Y2wLH4MLC74BV6FNDmdydBJukk76L833eksU9kL6UUP6XPek_f0nCR5jvIlIgV6t3WDt7Jdds7CMkeowDl5kJyjiuCMx_jhSXyWPAlhm-eMlJw_Ts4wKxjHJTlPfn1xNlPSewM-tdK6TvreqBZCKvV22Evbpzunh1b6tPOuB2PTvVTKWEhjKNMjn2nowGoYeWkt-KfJo0a2AZ5N70Xy4-OH75efs6vrT6vLi6tM8Qr3WcUrUtacQklyjJiONpqCAWF5jVW8cN3kGqumUHnZEK1rCZxwSrnSSDW6aMgieXnQ7VoXxNSVIBDnqCoLXLFIrA6EdnIrOm920v8RThpxe-D8WkwmhC4oVERDTRWhlFZVzaKE4sBkiTQaq72fqg31DrSKfr1sZ6LzL9ZsxNrtBSWM0ZJGgTeTgHc3A4Re7ExQ0LbSghtu742jw5zhiL76B73f3UStZTRgbONiXTWKiguKOSt5GedgkSzvoeKjYWdUHKHGxPNZwttZQmR6-N2v5RCCWH37-v_s9c85-_qE3YBs-01w7dAbZ8McpAdQeReCh-auySgX4wYcuyHGDRDTBsS0F6c_6C7pOPLkLyiDAXE</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1661987295</pqid></control><display><type>article</type><title>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Seth, Arjun ; Ritchie, Fiona K ; Wibowo, Nani ; Lua, Linda H L ; Middelberg, Anton P J</creator><contributor>Lu, Shan</contributor><creatorcontrib>Seth, Arjun ; Ritchie, Fiona K ; Wibowo, Nani ; Lua, Linda H L ; Middelberg, Anton P J ; Lu, Shan</creatorcontrib><description>Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117203</identifier><identifier>PMID: 25756283</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adjuvants, Immunologic - administration &amp; dosage ; Adjuvants, Immunologic - chemistry ; Adjuvants, Pharmaceutic - administration &amp; dosage ; Adjuvants, Pharmaceutic - chemistry ; Analysis ; Animals ; Antigens ; Biocompatibility ; Bioengineering ; Chemical properties ; Female ; Formulations ; Glycolic acid ; Health aspects ; Hepatitis A Antibodies - metabolism ; Immune response ; Immune system ; Immunogenicity ; In vivo methods and tests ; Influenza ; Influenza A ; Influenza Vaccines - administration &amp; dosage ; Influenza Vaccines - chemistry ; Influenza Vaccines - immunology ; Interferon ; Lactic Acid - chemistry ; Mice ; Mice, Inbred BALB C ; Nanoparticles ; Nanoparticles - administration &amp; dosage ; Nanoparticles - chemistry ; Nanotechnology ; Particle size ; Pathogens ; Peptides ; Pharmaceutical sciences ; Physicochemical properties ; Polyesters - chemistry ; Polyethylene glycol ; Polyglycolic Acid - chemistry ; Polylactic Acid-Polyglycolic Acid Copolymer ; Polylactide-co-glycolide ; Properties (attributes) ; Proteins ; Silica ; Silicon dioxide ; Silicon Dioxide - chemistry ; Splenocytes ; Studies ; Surface Properties ; Vaccines ; Viral Matrix Proteins - immunology ; Viruses</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0117203-e0117203</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Seth et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Seth et al 2015 Seth et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</citedby><cites>FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355484/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355484/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25756283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lu, Shan</contributor><creatorcontrib>Seth, Arjun</creatorcontrib><creatorcontrib>Ritchie, Fiona K</creatorcontrib><creatorcontrib>Wibowo, Nani</creatorcontrib><creatorcontrib>Lua, Linda H L</creatorcontrib><creatorcontrib>Middelberg, Anton P J</creatorcontrib><title>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</description><subject>Adjuvants, Immunologic - administration &amp; dosage</subject><subject>Adjuvants, Immunologic - chemistry</subject><subject>Adjuvants, Pharmaceutic - administration &amp; dosage</subject><subject>Adjuvants, Pharmaceutic - chemistry</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antigens</subject><subject>Biocompatibility</subject><subject>Bioengineering</subject><subject>Chemical properties</subject><subject>Female</subject><subject>Formulations</subject><subject>Glycolic acid</subject><subject>Health aspects</subject><subject>Hepatitis A Antibodies - metabolism</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunogenicity</subject><subject>In vivo methods and tests</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza Vaccines - administration &amp; dosage</subject><subject>Influenza Vaccines - chemistry</subject><subject>Influenza Vaccines - immunology</subject><subject>Interferon</subject><subject>Lactic Acid - chemistry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanoparticles</subject><subject>Nanoparticles - administration &amp; dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Nanotechnology</subject><subject>Particle size</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>Pharmaceutical sciences</subject><subject>Physicochemical properties</subject><subject>Polyesters - chemistry</subject><subject>Polyethylene glycol</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer</subject><subject>Polylactide-co-glycolide</subject><subject>Properties (attributes)</subject><subject>Proteins</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Silicon Dioxide - chemistry</subject><subject>Splenocytes</subject><subject>Studies</subject><subject>Surface Properties</subject><subject>Vaccines</subject><subject>Viral Matrix Proteins - immunology</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7jr6D0QLguhFx-az7Y2wLH4MLC74BV6FNDmdydBJukk76L833eksU9kL6UUP6XPek_f0nCR5jvIlIgV6t3WDt7Jdds7CMkeowDl5kJyjiuCMx_jhSXyWPAlhm-eMlJw_Ts4wKxjHJTlPfn1xNlPSewM-tdK6TvreqBZCKvV22Evbpzunh1b6tPOuB2PTvVTKWEhjKNMjn2nowGoYeWkt-KfJo0a2AZ5N70Xy4-OH75efs6vrT6vLi6tM8Qr3WcUrUtacQklyjJiONpqCAWF5jVW8cN3kGqumUHnZEK1rCZxwSrnSSDW6aMgieXnQ7VoXxNSVIBDnqCoLXLFIrA6EdnIrOm920v8RThpxe-D8WkwmhC4oVERDTRWhlFZVzaKE4sBkiTQaq72fqg31DrSKfr1sZ6LzL9ZsxNrtBSWM0ZJGgTeTgHc3A4Re7ExQ0LbSghtu742jw5zhiL76B73f3UStZTRgbONiXTWKiguKOSt5GedgkSzvoeKjYWdUHKHGxPNZwttZQmR6-N2v5RCCWH37-v_s9c85-_qE3YBs-01w7dAbZ8McpAdQeReCh-auySgX4wYcuyHGDRDTBsS0F6c_6C7pOPLkLyiDAXE</recordid><startdate>20150310</startdate><enddate>20150310</enddate><creator>Seth, Arjun</creator><creator>Ritchie, Fiona K</creator><creator>Wibowo, Nani</creator><creator>Lua, Linda H L</creator><creator>Middelberg, Anton P J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150310</creationdate><title>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</title><author>Seth, Arjun ; Ritchie, Fiona K ; Wibowo, Nani ; Lua, Linda H L ; Middelberg, Anton P J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-96938b64e830215d720f75e350b2c053bf0d2cf7c08f3ddbae636446cd1cfd7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adjuvants, Immunologic - administration &amp; dosage</topic><topic>Adjuvants, Immunologic - chemistry</topic><topic>Adjuvants, Pharmaceutic - administration &amp; dosage</topic><topic>Adjuvants, Pharmaceutic - chemistry</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antigens</topic><topic>Biocompatibility</topic><topic>Bioengineering</topic><topic>Chemical properties</topic><topic>Female</topic><topic>Formulations</topic><topic>Glycolic acid</topic><topic>Health aspects</topic><topic>Hepatitis A Antibodies - metabolism</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunogenicity</topic><topic>In vivo methods and tests</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Influenza Vaccines - administration &amp; dosage</topic><topic>Influenza Vaccines - chemistry</topic><topic>Influenza Vaccines - immunology</topic><topic>Interferon</topic><topic>Lactic Acid - chemistry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanoparticles</topic><topic>Nanoparticles - administration &amp; dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Nanotechnology</topic><topic>Particle size</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>Pharmaceutical sciences</topic><topic>Physicochemical properties</topic><topic>Polyesters - chemistry</topic><topic>Polyethylene glycol</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer</topic><topic>Polylactide-co-glycolide</topic><topic>Properties (attributes)</topic><topic>Proteins</topic><topic>Silica</topic><topic>Silicon dioxide</topic><topic>Silicon Dioxide - chemistry</topic><topic>Splenocytes</topic><topic>Studies</topic><topic>Surface Properties</topic><topic>Vaccines</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seth, Arjun</creatorcontrib><creatorcontrib>Ritchie, Fiona K</creatorcontrib><creatorcontrib>Wibowo, Nani</creatorcontrib><creatorcontrib>Lua, Linda H L</creatorcontrib><creatorcontrib>Middelberg, Anton P J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seth, Arjun</au><au>Ritchie, Fiona K</au><au>Wibowo, Nani</au><au>Lua, Linda H L</au><au>Middelberg, Anton P J</au><au>Lu, Shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-10</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0117203</spage><epage>e0117203</epage><pages>e0117203-e0117203</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Nanoparticles are increasingly used to adjuvant vaccine formulations due to their biocompatibility, ease of manufacture and the opportunity to tailor their size, shape, and physicochemical properties. The efficacy of similarly-sized silica (Si-OH), poly (D,L-lactic-co-glycolic acid) (PLGA) and poly caprolactone (PCL) nanoparticles (nps) to adjuvant recombinant capsomere presenting antigenic M2e modular peptide from Influenza A virus (CapM2e) was investigated in vivo. Formulation of CapM2e with Si-OH or PLGA nps significantly boosted the immunogenicity of modular capsomeres, even though CapM2e was not actively attached to the nanoparticles prior to injection (i.e., formulation was by simple mixing). In contrast, PCL nps showed no significant adjuvant effect using this simple-mixing approach. The immune response induced by CapM2e alone or formulated with nps was antibody-biased with very high antigen-specific antibody titer and less than 20 cells per million splenocytes secreting interferon gamma. Modification of silica nanoparticle surface properties through amine functionalization and pegylation did not lead to significant changes in immune response. This study confirms that simple mixing-based formulation can lead to effective adjuvanting of antigenic protein, though with antibody titer dependent on nanoparticle physicochemical properties.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25756283</pmid><doi>10.1371/journal.pone.0117203</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-03, Vol.10 (3), p.e0117203-e0117203
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1661987295
source MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry
subjects Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - chemistry
Adjuvants, Pharmaceutic - administration & dosage
Adjuvants, Pharmaceutic - chemistry
Analysis
Animals
Antigens
Biocompatibility
Bioengineering
Chemical properties
Female
Formulations
Glycolic acid
Health aspects
Hepatitis A Antibodies - metabolism
Immune response
Immune system
Immunogenicity
In vivo methods and tests
Influenza
Influenza A
Influenza Vaccines - administration & dosage
Influenza Vaccines - chemistry
Influenza Vaccines - immunology
Interferon
Lactic Acid - chemistry
Mice
Mice, Inbred BALB C
Nanoparticles
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Nanotechnology
Particle size
Pathogens
Peptides
Pharmaceutical sciences
Physicochemical properties
Polyesters - chemistry
Polyethylene glycol
Polyglycolic Acid - chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Polylactide-co-glycolide
Properties (attributes)
Proteins
Silica
Silicon dioxide
Silicon Dioxide - chemistry
Splenocytes
Studies
Surface Properties
Vaccines
Viral Matrix Proteins - immunology
Viruses
title Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T16%3A25%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-carrier%20nanoparticles%20adjuvant%20modular%20protein%20vaccine%20in%20a%20particle-dependent%20manner&rft.jtitle=PloS%20one&rft.au=Seth,%20Arjun&rft.date=2015-03-10&rft.volume=10&rft.issue=3&rft.spage=e0117203&rft.epage=e0117203&rft.pages=e0117203-e0117203&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117203&rft_dat=%3Cgale_plos_%3EA426586819%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1661987295&rft_id=info:pmid/25756283&rft_galeid=A426586819&rft_doaj_id=oai_doaj_org_article_d74e93deb4c344499b5729c6e5a81d1f&rfr_iscdi=true