Alcohol induced alterations to the human fecal VOC metabolome
Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and tox...
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| Veröffentlicht in: | PloS one 2015-03, Vol.10 (3), p.e0119362 |
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| description | Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies. |
| doi_str_mv | 10.1371/journal.pone.0119362 |
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However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119362</identifier><identifier>PMID: 25751150</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Alcoholic beverages ; Alcoholic liver diseases ; Alcoholics ; Alcoholism ; Alcohols ; Alkanes - metabolism ; Bicyclic Monoterpenes ; Bioindicators ; Biomarkers ; Camphene ; Care and treatment ; Caryophyllene ; Case-Control Studies ; Complications and side effects ; Dimethyl trisulfide ; Disulfides - metabolism ; Drinking (Alcoholic beverages) ; Dysbacteriosis ; Epithelial cells ; Ethanol ; Ethanol - adverse effects ; Failure analysis ; Fatty acids ; Fatty Acids, Volatile - metabolism ; Fatty Alcohols - metabolism ; Fatty liver ; Feces - chemistry ; Feces - microbiology ; Female ; Health aspects ; Homeostasis ; Humans ; Influence ; Intestinal microflora ; Intestine ; Male ; Metabolites ; Metabolome - drug effects ; Metabolomics ; Microbiota ; Microbiota (Symbiotic organisms) ; Microbiota - drug effects ; Microorganisms ; Middle Aged ; Nutrition ; Organic compounds ; Oxidative stress ; Physiological aspects ; Polycyclic Sesquiterpenes ; Propionic acid ; Sesquiterpenes - metabolism ; Steatosis ; Studies ; Sulfides - metabolism ; Terpenes - metabolism ; Tetradecane ; VOCs ; Volatile organic compounds ; Volatile Organic Compounds - metabolism ; Young Adult</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119362</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Couch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Couch et al 2015 Couch et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-fabd04a1c4f12a5920a4a223d6e113af2060f78f5bcbae79e760fca00127a7db3</citedby><cites>FETCH-LOGICAL-c692t-fabd04a1c4f12a5920a4a223d6e113af2060f78f5bcbae79e760fca00127a7db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353727/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353727/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25751150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Heimesaat, Markus M.</contributor><creatorcontrib>Couch, Robin D</creatorcontrib><creatorcontrib>Dailey, Allyson</creatorcontrib><creatorcontrib>Zaidi, Fatima</creatorcontrib><creatorcontrib>Navarro, Karl</creatorcontrib><creatorcontrib>Forsyth, Christopher B</creatorcontrib><creatorcontrib>Mutlu, Ece</creatorcontrib><creatorcontrib>Engen, Phillip A</creatorcontrib><creatorcontrib>Keshavarzian, Ali</creatorcontrib><title>Alcohol induced alterations to the human fecal VOC metabolome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Studies have shown that excessive alcohol consumption impacts the intestinal microbiota composition, causing disruption of homeostasis (dysbiosis). However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. 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However, this observed change is not indicative of the dysbiotic intestinal microbiota function that could result in the production of injurious and toxic products. Thus, knowledge of the effects of alcohol on the intestinal microbiota function and their metabolites is warranted, in order to better understand the role of the intestinal microbiota in alcohol associated organ failure. Here, we report the results of a differential metabolomic analysis comparing volatile organic compounds (VOC) detected in the stool of alcoholics and non-alcoholic healthy controls. We performed the analysis with fecal samples collected after passage as well as with samples collected directly from the sigmoid lumen. Regardless of the approach to fecal collection, we found a stool VOC metabolomic signature in alcoholics that is different from healthy controls. The most notable metabolite alterations in the alcoholic samples include: (1) an elevation in the oxidative stress biomarker tetradecane; (2) a decrease in five fatty alcohols with anti-oxidant property; (3) a decrease in the short chain fatty acids propionate and isobutyrate, important in maintaining intestinal epithelial cell health and barrier integrity; (4) a decrease in alcohol consumption natural suppressant caryophyllene; (5) a decrease in natural product and hepatic steatosis attenuator camphene; and (6) decreased dimethyl disulfide and dimethyl trisulfide, microbial products of decomposition. Our results showed that intestinal microbiota function is altered in alcoholics which might promote alcohol associated pathologies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25751150</pmid><doi>10.1371/journal.pone.0119362</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-03, Vol.10 (3), p.e0119362 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1661596293 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Alcoholic beverages Alcoholic liver diseases Alcoholics Alcoholism Alcohols Alkanes - metabolism Bicyclic Monoterpenes Bioindicators Biomarkers Camphene Care and treatment Caryophyllene Case-Control Studies Complications and side effects Dimethyl trisulfide Disulfides - metabolism Drinking (Alcoholic beverages) Dysbacteriosis Epithelial cells Ethanol Ethanol - adverse effects Failure analysis Fatty acids Fatty Acids, Volatile - metabolism Fatty Alcohols - metabolism Fatty liver Feces - chemistry Feces - microbiology Female Health aspects Homeostasis Humans Influence Intestinal microflora Intestine Male Metabolites Metabolome - drug effects Metabolomics Microbiota Microbiota (Symbiotic organisms) Microbiota - drug effects Microorganisms Middle Aged Nutrition Organic compounds Oxidative stress Physiological aspects Polycyclic Sesquiterpenes Propionic acid Sesquiterpenes - metabolism Steatosis Studies Sulfides - metabolism Terpenes - metabolism Tetradecane VOCs Volatile organic compounds Volatile Organic Compounds - metabolism Young Adult |
| title | Alcohol induced alterations to the human fecal VOC metabolome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T09%3A31%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Alcohol%20induced%20alterations%20to%20the%20human%20fecal%20VOC%20metabolome&rft.jtitle=PloS%20one&rft.au=Couch,%20Robin%20D&rft.date=2015-03-09&rft.volume=10&rft.issue=3&rft.spage=e0119362&rft.pages=e0119362-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0119362&rft_dat=%3Cgale_plos_%3EA429254246%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1661596293&rft_id=info:pmid/25751150&rft_galeid=A429254246&rft_doaj_id=oai_doaj_org_article_50135bc6018e4337aa04a8dd8813f8de&rfr_iscdi=true |