Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression
The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP) in specific subsets of peripheral and central neurons. The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure an...
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| creator | Taylor-Clark, Thomas E Wu, Kevin Y Thompson, Julie-Ann Yang, Kiseok Bahia, Parmvir K Ajmo, Joanne M |
| description | The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP) in specific subsets of peripheral and central neurons. The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure and function. Here, we have characterized the expression of YFP in the sensory ganglia (DRG, trigeminal and vagal) of the Thy1.2 YFP-16 mouse, using biochemical, functional and anatomical analyses. Despite previous reports, we found that YFP was only expressed in approximately half of DRG and trigeminal neurons and less than 10% of vagal neurons. YFP-expression was only found in medium and large-diameter neurons that expressed neurofilament but not TRPV1. YFP-expressing neurons failed to respond to selective agonists for TRPV1, P2X(2/3 and TRPM8 channels in Ca2+ imaging assays. Confocal analysis of glabrous skin, hairy skin of the back and ear and skeletal muscle indicated that YFP was expressed in some peripheral terminals with structures consistent with their presumed non-nociceptive nature. In summary, the Thy1.2 YFP-16 mouse expresses robust YFP expression in only a subset of sensory neurons. But this mouse model is not suitable for the study of nociceptive nerves or the function of such nerves in pain and neuropathies. |
| doi_str_mv | 10.1371/journal.pone.0119538 |
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The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure and function. Here, we have characterized the expression of YFP in the sensory ganglia (DRG, trigeminal and vagal) of the Thy1.2 YFP-16 mouse, using biochemical, functional and anatomical analyses. Despite previous reports, we found that YFP was only expressed in approximately half of DRG and trigeminal neurons and less than 10% of vagal neurons. YFP-expression was only found in medium and large-diameter neurons that expressed neurofilament but not TRPV1. YFP-expressing neurons failed to respond to selective agonists for TRPV1, P2X(2/3 and TRPM8 channels in Ca2+ imaging assays. Confocal analysis of glabrous skin, hairy skin of the back and ear and skeletal muscle indicated that YFP was expressed in some peripheral terminals with structures consistent with their presumed non-nociceptive nature. In summary, the Thy1.2 YFP-16 mouse expresses robust YFP expression in only a subset of sensory neurons. But this mouse model is not suitable for the study of nociceptive nerves or the function of such nerves in pain and neuropathies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119538</identifier><identifier>PMID: 25746468</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Calcium channels ; Calcium imaging ; Capsaicin receptors ; Fluorescence ; Ganglia ; Ganglia, Spinal - cytology ; Ganglia, Spinal - metabolism ; Genetic aspects ; Ion channels ; Luminescent Proteins - genetics ; Lungs ; Medicine ; Mice ; Mice, Transgenic ; Muscles ; Musculoskeletal system ; Nerves ; Neurons ; Neuropathy ; Pain ; Pain perception ; Peripheral neuropathy ; Pharmacology ; Physiology ; Proteins ; Rodents ; Sensory neurons ; Sensory Receptor Cells - metabolism ; Sensory receptors ; Skeletal muscle ; Skin ; Structure-function relationships ; Thy-1 Antigens - genetics ; Transgenic animals ; Transgenic mice ; Transient receptor potential proteins ; TRPV Cation Channels - genetics ; TRPV Cation Channels - metabolism ; Vagus nerve ; Yellow fluorescent protein ; Yellow fluorescent proteins</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119538-e0119538</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Taylor-Clark et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Taylor-Clark et al 2015 Taylor-Clark et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b05b8e027e910a54c1699ed2f75d98222ad2b7049463b61411572c0f3fb8163e3</citedby><cites>FETCH-LOGICAL-c692t-b05b8e027e910a54c1699ed2f75d98222ad2b7049463b61411572c0f3fb8163e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351979/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351979/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25746468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Costigan, Michael</contributor><creatorcontrib>Taylor-Clark, Thomas E</creatorcontrib><creatorcontrib>Wu, Kevin Y</creatorcontrib><creatorcontrib>Thompson, Julie-Ann</creatorcontrib><creatorcontrib>Yang, Kiseok</creatorcontrib><creatorcontrib>Bahia, Parmvir K</creatorcontrib><creatorcontrib>Ajmo, Joanne M</creatorcontrib><title>Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP) in specific subsets of peripheral and central neurons. The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure and function. Here, we have characterized the expression of YFP in the sensory ganglia (DRG, trigeminal and vagal) of the Thy1.2 YFP-16 mouse, using biochemical, functional and anatomical analyses. Despite previous reports, we found that YFP was only expressed in approximately half of DRG and trigeminal neurons and less than 10% of vagal neurons. YFP-expression was only found in medium and large-diameter neurons that expressed neurofilament but not TRPV1. YFP-expressing neurons failed to respond to selective agonists for TRPV1, P2X(2/3 and TRPM8 channels in Ca2+ imaging assays. Confocal analysis of glabrous skin, hairy skin of the back and ear and skeletal muscle indicated that YFP was expressed in some peripheral terminals with structures consistent with their presumed non-nociceptive nature. In summary, the Thy1.2 YFP-16 mouse expresses robust YFP expression in only a subset of sensory neurons. But this mouse model is not suitable for the study of nociceptive nerves or the function of such nerves in pain and neuropathies.</description><subject>Animals</subject><subject>Calcium channels</subject><subject>Calcium imaging</subject><subject>Capsaicin receptors</subject><subject>Fluorescence</subject><subject>Ganglia</subject><subject>Ganglia, Spinal - cytology</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Genetic aspects</subject><subject>Ion channels</subject><subject>Luminescent Proteins - genetics</subject><subject>Lungs</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Nerves</subject><subject>Neurons</subject><subject>Neuropathy</subject><subject>Pain</subject><subject>Pain perception</subject><subject>Peripheral neuropathy</subject><subject>Pharmacology</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Sensory neurons</subject><subject>Sensory Receptor Cells - metabolism</subject><subject>Sensory receptors</subject><subject>Skeletal muscle</subject><subject>Skin</subject><subject>Structure-function relationships</subject><subject>Thy-1 Antigens - genetics</subject><subject>Transgenic animals</subject><subject>Transgenic mice</subject><subject>Transient receptor potential proteins</subject><subject>TRPV Cation Channels - genetics</subject><subject>TRPV Cation Channels - metabolism</subject><subject>Vagus nerve</subject><subject>Yellow fluorescent protein</subject><subject>Yellow fluorescent proteins</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIHoxaz5mMpMboRSrC4WWuha8CpnMmdlpZ5NtkpHuvzfbnZYd6YXkIuHkOW9OzkeSvCV4RlhBvlzbwRnVz9bWwAwTInJWPksOiWA05RSz53vng-SV99cYR4Tzl8kBzYuMZ7w8TG4Wyw2ZUfT79CIlHAWnjG_BdBqt7OAB9aqC3iOF_FB5CMg20eRaSOtOrSCAQx6Mt26DDAzOGo_CUoXI6Bu0uLy4Igju1g6876x5nbxoVO_hzbgfJb9Ovy1OfqRn59_nJ8dnqeaChrTCeVUCpgUIglWeacKFgJo2RV6LklKqaloVOBMZZxUnGSF5QTVuWFOVhDNgR8n7ne66t16OefKScE4IpjQXkZjviNqqa7l23Uq5jbSqk_cG61qpXOh0DxLXROBccCpIDAWYaKjKCW5oWZWN0lXU-jq-NlQrqDWYmMR-Ijq9Md1StvaPzFhORLEN5tMo4OztAD7IVec19L0yEGtwH7coy4KziH74B336dyPVqviBzjQ2vqu3ovI4o6IoSiZIpGZPUHHVsOp0bKqmi_aJw-eJQ2QC3IVWDd7L-c_L_2fPr6bsxz12CaoPS2_7IcSW8VMw24HaWe8dNI9JJlhuZ-IhG3I7E3Kciej2br9Aj04PQ8D-AuEUBJc</recordid><startdate>20150306</startdate><enddate>20150306</enddate><creator>Taylor-Clark, Thomas E</creator><creator>Wu, Kevin Y</creator><creator>Thompson, Julie-Ann</creator><creator>Yang, Kiseok</creator><creator>Bahia, Parmvir K</creator><creator>Ajmo, Joanne M</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150306</creationdate><title>Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression</title><author>Taylor-Clark, Thomas E ; Wu, Kevin Y ; Thompson, Julie-Ann ; Yang, Kiseok ; Bahia, Parmvir K ; Ajmo, Joanne M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b05b8e027e910a54c1699ed2f75d98222ad2b7049463b61411572c0f3fb8163e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Calcium channels</topic><topic>Calcium imaging</topic><topic>Capsaicin receptors</topic><topic>Fluorescence</topic><topic>Ganglia</topic><topic>Ganglia, Spinal - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taylor-Clark, Thomas E</au><au>Wu, Kevin Y</au><au>Thompson, Julie-Ann</au><au>Yang, Kiseok</au><au>Bahia, Parmvir K</au><au>Ajmo, Joanne M</au><au>Costigan, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-06</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0119538</spage><epage>e0119538</epage><pages>e0119538-e0119538</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The Thy1.2 YFP-16 mouse expresses yellow fluorescent protein (YFP) in specific subsets of peripheral and central neurons. The original characterization of this model suggested that YFP was expressed in all sensory neurons, and this model has been subsequently used to study sensory nerve structure and function. Here, we have characterized the expression of YFP in the sensory ganglia (DRG, trigeminal and vagal) of the Thy1.2 YFP-16 mouse, using biochemical, functional and anatomical analyses. Despite previous reports, we found that YFP was only expressed in approximately half of DRG and trigeminal neurons and less than 10% of vagal neurons. YFP-expression was only found in medium and large-diameter neurons that expressed neurofilament but not TRPV1. YFP-expressing neurons failed to respond to selective agonists for TRPV1, P2X(2/3 and TRPM8 channels in Ca2+ imaging assays. Confocal analysis of glabrous skin, hairy skin of the back and ear and skeletal muscle indicated that YFP was expressed in some peripheral terminals with structures consistent with their presumed non-nociceptive nature. In summary, the Thy1.2 YFP-16 mouse expresses robust YFP expression in only a subset of sensory neurons. But this mouse model is not suitable for the study of nociceptive nerves or the function of such nerves in pain and neuropathies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25746468</pmid><doi>10.1371/journal.pone.0119538</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Calcium channels Calcium imaging Capsaicin receptors Fluorescence Ganglia Ganglia, Spinal - cytology Ganglia, Spinal - metabolism Genetic aspects Ion channels Luminescent Proteins - genetics Lungs Medicine Mice Mice, Transgenic Muscles Musculoskeletal system Nerves Neurons Neuropathy Pain Pain perception Peripheral neuropathy Pharmacology Physiology Proteins Rodents Sensory neurons Sensory Receptor Cells - metabolism Sensory receptors Skeletal muscle Skin Structure-function relationships Thy-1 Antigens - genetics Transgenic animals Transgenic mice Transient receptor potential proteins TRPV Cation Channels - genetics TRPV Cation Channels - metabolism Vagus nerve Yellow fluorescent protein Yellow fluorescent proteins |
| title | Thy1.2 YFP-16 transgenic mouse labels a subset of large-diameter sensory neurons that lack TRPV1 expression |
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