Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes
Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estima...
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| creator | Duong, Yen T Kassanjee, Reshma Welte, Alex Morgan, Meade De, Anindya Dobbs, Trudy Rottinghaus, Erin Nkengasong, John Curlin, Marcel E Kittinunvorakoon, Chonticha Raengsakulrach, Boonyos Martin, Michael Choopanya, Kachit Vanichseni, Suphak Jiang, Yan Qiu, Maofeng Yu, Haiying Hao, Yan Shah, Neha Le, Linh-Vi Kim, Andrea A Nguyen, Tuan Anh Ampofo, William Parekh, Bharat S |
| description | Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus.
A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.
Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR ( |
| doi_str_mv | 10.1371/journal.pone.0114947 |
| format | Article |
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A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.
Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C).
Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0114947</identifier><identifier>PMID: 25710171</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; AIDS ; Antibody Affinity ; Avidity ; Calibration ; Data analysis ; Data processing ; Disease control ; Epidemiology ; HIV ; HIV Antigens - immunology ; HIV Seropositivity - diagnosis ; HIV Seropositivity - epidemiology ; HIV-1 - classification ; HIV-1 - genetics ; HIV-1 - immunology ; Human immunodeficiency virus ; Humans ; Immunoenzyme Techniques - standards ; Incidence ; Infections ; Parameter estimation ; Seroconversion ; Serologic Tests - standards ; Statistical methods ; Statistics</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0114947-e0114947</ispartof><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”) Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-f8e1c94767d088f134409017b59e1c273636478b397d9e94a25982d8584851733</citedby><cites>FETCH-LOGICAL-c592t-f8e1c94767d088f134409017b59e1c273636478b397d9e94a25982d8584851733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339840/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339840/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25710171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ceccherini-Silberstein, Francesca</contributor><creatorcontrib>Duong, Yen T</creatorcontrib><creatorcontrib>Kassanjee, Reshma</creatorcontrib><creatorcontrib>Welte, Alex</creatorcontrib><creatorcontrib>Morgan, Meade</creatorcontrib><creatorcontrib>De, Anindya</creatorcontrib><creatorcontrib>Dobbs, Trudy</creatorcontrib><creatorcontrib>Rottinghaus, Erin</creatorcontrib><creatorcontrib>Nkengasong, John</creatorcontrib><creatorcontrib>Curlin, Marcel E</creatorcontrib><creatorcontrib>Kittinunvorakoon, Chonticha</creatorcontrib><creatorcontrib>Raengsakulrach, Boonyos</creatorcontrib><creatorcontrib>Martin, Michael</creatorcontrib><creatorcontrib>Choopanya, Kachit</creatorcontrib><creatorcontrib>Vanichseni, Suphak</creatorcontrib><creatorcontrib>Jiang, Yan</creatorcontrib><creatorcontrib>Qiu, Maofeng</creatorcontrib><creatorcontrib>Yu, Haiying</creatorcontrib><creatorcontrib>Hao, Yan</creatorcontrib><creatorcontrib>Shah, Neha</creatorcontrib><creatorcontrib>Le, Linh-Vi</creatorcontrib><creatorcontrib>Kim, Andrea A</creatorcontrib><creatorcontrib>Nguyen, Tuan Anh</creatorcontrib><creatorcontrib>Ampofo, William</creatorcontrib><creatorcontrib>Parekh, Bharat S</creatorcontrib><title>Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus.
A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.
Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C).
Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use.</description><subject>Acquired immune deficiency syndrome</subject><subject>AIDS</subject><subject>Antibody Affinity</subject><subject>Avidity</subject><subject>Calibration</subject><subject>Data analysis</subject><subject>Data processing</subject><subject>Disease control</subject><subject>Epidemiology</subject><subject>HIV</subject><subject>HIV Antigens - immunology</subject><subject>HIV Seropositivity - diagnosis</subject><subject>HIV Seropositivity - epidemiology</subject><subject>HIV-1 - classification</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunoenzyme Techniques - standards</subject><subject>Incidence</subject><subject>Infections</subject><subject>Parameter estimation</subject><subject>Seroconversion</subject><subject>Serologic Tests - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duong, Yen T</au><au>Kassanjee, Reshma</au><au>Welte, Alex</au><au>Morgan, Meade</au><au>De, Anindya</au><au>Dobbs, Trudy</au><au>Rottinghaus, Erin</au><au>Nkengasong, John</au><au>Curlin, Marcel E</au><au>Kittinunvorakoon, Chonticha</au><au>Raengsakulrach, Boonyos</au><au>Martin, Michael</au><au>Choopanya, Kachit</au><au>Vanichseni, Suphak</au><au>Jiang, Yan</au><au>Qiu, Maofeng</au><au>Yu, Haiying</au><au>Hao, Yan</au><au>Shah, Neha</au><au>Le, Linh-Vi</au><au>Kim, Andrea A</au><au>Nguyen, Tuan Anh</au><au>Ampofo, William</au><au>Parekh, Bharat S</au><au>Ceccherini-Silberstein, Francesca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-24</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0114947</spage><epage>e0114947</epage><pages>e0114947-e0114947</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mean duration of recent infection (MDRI) and misclassification of long-term HIV-1 infections, as proportion false recent (PFR), are critical parameters for laboratory-based assays for estimating HIV-1 incidence. Recent review of the data by us and others indicated that MDRI of LAg-Avidity EIA estimated previously required recalibration. We present here results of recalibration efforts using >250 seroconversion panels and multiple statistical methods to ensure accuracy and consensus.
A total of 2737 longitudinal specimens collected from 259 seroconverting individuals infected with diverse HIV-1 subtypes were tested with the LAg-Avidity EIA as previously described. Data were analyzed for determination of MDRI at ODn cutoffs of 1.0 to 2.0 using 7 statistical approaches and sub-analyzed by HIV-1 subtypes. In addition, 3740 specimens from individuals with infection >1 year, including 488 from patients with AIDS, were tested for PFR at varying cutoffs.
Using different statistical methods, MDRI values ranged from 88-94 days at cutoff ODn = 1.0 to 177-183 days at ODn = 2.0. The MDRI values were similar by different methods suggesting coherence of different approaches. Testing for misclassification among long-term infections indicated that overall PFRs were 0.6% to 2.5% at increasing cutoffs of 1.0 to 2.0, respectively. Balancing the need for a longer MDRI and smaller PFR (<2.0%) suggests that a cutoff ODn = 1.5, corresponding to an MDRI of 130 days should be used for cross-sectional application. The MDRI varied among subtypes from 109 days (subtype A&D) to 152 days (subtype C).
Based on the new data and revised analysis, we recommend an ODn cutoff = 1.5 to classify recent and long-term infections, corresponding to an MDRI of 130 days (118-142). Determination of revised parameters for estimation of HIV-1 incidence should facilitate application of the LAg-Avidity EIA for worldwide use.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25710171</pmid><doi>10.1371/journal.pone.0114947</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-02, Vol.10 (2), p.e0114947-e0114947 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1658458329 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acquired immune deficiency syndrome AIDS Antibody Affinity Avidity Calibration Data analysis Data processing Disease control Epidemiology HIV HIV Antigens - immunology HIV Seropositivity - diagnosis HIV Seropositivity - epidemiology HIV-1 - classification HIV-1 - genetics HIV-1 - immunology Human immunodeficiency virus Humans Immunoenzyme Techniques - standards Incidence Infections Parameter estimation Seroconversion Serologic Tests - standards Statistical methods Statistics |
| title | Recalibration of the limiting antigen avidity EIA to determine mean duration of recent infection in divergent HIV-1 subtypes |
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