A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis
Heterotrimeric G-proteins are versatile regulators involved in diverse cellular processes in eukaryotes. In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this...
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| creator | Xu, Dong-bei Chen, Ming Ma, Ya-nan Xu, Zhao-shi Li, Lian-cheng Chen, Yao-feng Ma, You-zhi |
| description | Heterotrimeric G-proteins are versatile regulators involved in diverse cellular processes in eukaryotes. In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate drought tolerance in Arabidopsis. Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling. Our study found that transcript levels of three ABA responsive genes, AtMPK6, AtVIP1 and AtMYB44 (downstream gene of AtVIP1), were significantly up-regulated in agb1-2 lines after ABA or drought treatments. Other ABA-responsive and drought-inducible genes, such as RD29A (downstream gene of AtMYB44), were also up-regulated in agb1-2 lines. Furthermore, overexpression of AtVIP1 resulted in hypersensitivity to ABA at seed germination and seedling stages, and significantly enhanced drought tolerance in transgenic plants. These results suggest that AGB1 was involved in the ABA signaling pathway and drought tolerance in Arabidopsis through down-regulating the AtMPK6, AtVIP1 and AtMYB44 cascade. |
| doi_str_mv | 10.1371/journal.pone.0116385 |
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In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate drought tolerance in Arabidopsis. Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling. Our study found that transcript levels of three ABA responsive genes, AtMPK6, AtVIP1 and AtMYB44 (downstream gene of AtVIP1), were significantly up-regulated in agb1-2 lines after ABA or drought treatments. Other ABA-responsive and drought-inducible genes, such as RD29A (downstream gene of AtMYB44), were also up-regulated in agb1-2 lines. Furthermore, overexpression of AtVIP1 resulted in hypersensitivity to ABA at seed germination and seedling stages, and significantly enhanced drought tolerance in transgenic plants. These results suggest that AGB1 was involved in the ABA signaling pathway and drought tolerance in Arabidopsis through down-regulating the AtMPK6, AtVIP1 and AtMYB44 cascade.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0116385</identifier><identifier>PMID: 25635681</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abscisic acid ; Abscisic Acid - physiology ; Agriculture ; Arabidopsis ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis Proteins - physiology ; Biology ; Crop science ; Dehydration - metabolism ; Down-Regulation ; Drought ; Drought resistance ; Droughts ; Eukaryotes ; Gene Expression ; Gene Expression Regulation, Plant ; Genes ; Germination ; GTP-Binding Protein beta Subunits - physiology ; Hypersensitivity ; Kinases ; Laboratories ; Mitogen-Activated Protein Kinases - genetics ; Mitogen-Activated Protein Kinases - metabolism ; Molecular modelling ; Plant Growth Regulators - physiology ; Protein kinase ; Proteins ; Regulators ; Seed germination ; Seedlings ; Seeds ; Signal Transduction ; Signaling ; Transcription factors ; Transgenic plants ; Triticeae</subject><ispartof>PloS one, 2015, Vol.10 (1), p.e0116385-e0116385</ispartof><rights>2015 Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Xu et al 2015 Xu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-87a2be61dbcc811956f5c1c5288cd79114fb99c566a803db0d37e5cdd7a484743</citedby><cites>FETCH-LOGICAL-c526t-87a2be61dbcc811956f5c1c5288cd79114fb99c566a803db0d37e5cdd7a484743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312036/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312036/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,4028,23875,27932,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25635681$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Hong</contributor><creatorcontrib>Xu, Dong-bei</creatorcontrib><creatorcontrib>Chen, Ming</creatorcontrib><creatorcontrib>Ma, Ya-nan</creatorcontrib><creatorcontrib>Xu, Zhao-shi</creatorcontrib><creatorcontrib>Li, Lian-cheng</creatorcontrib><creatorcontrib>Chen, Yao-feng</creatorcontrib><creatorcontrib>Ma, You-zhi</creatorcontrib><title>A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Heterotrimeric G-proteins are versatile regulators involved in diverse cellular processes in eukaryotes. In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate drought tolerance in Arabidopsis. Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling. Our study found that transcript levels of three ABA responsive genes, AtMPK6, AtVIP1 and AtMYB44 (downstream gene of AtVIP1), were significantly up-regulated in agb1-2 lines after ABA or drought treatments. Other ABA-responsive and drought-inducible genes, such as RD29A (downstream gene of AtMYB44), were also up-regulated in agb1-2 lines. Furthermore, overexpression of AtVIP1 resulted in hypersensitivity to ABA at seed germination and seedling stages, and significantly enhanced drought tolerance in transgenic plants. These results suggest that AGB1 was involved in the ABA signaling pathway and drought tolerance in Arabidopsis through down-regulating the AtMPK6, AtVIP1 and AtMYB44 cascade.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - physiology</subject><subject>Agriculture</subject><subject>Arabidopsis</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Biology</subject><subject>Crop science</subject><subject>Dehydration - metabolism</subject><subject>Down-Regulation</subject><subject>Drought</subject><subject>Drought resistance</subject><subject>Droughts</subject><subject>Eukaryotes</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Germination</subject><subject>GTP-Binding Protein beta Subunits - physiology</subject><subject>Hypersensitivity</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Mitogen-Activated Protein Kinases - genetics</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Molecular modelling</subject><subject>Plant Growth Regulators - physiology</subject><subject>Protein kinase</subject><subject>Proteins</subject><subject>Regulators</subject><subject>Seed germination</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Transcription factors</subject><subject>Transgenic plants</subject><subject>Triticeae</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1u1DAUjhCIlsINEFhiw6IZ7PgnyQYprWCoKIIFrC3Hfsl4lLEH22k11-AoHIQzkemkVYtY2Xrv-3nP_rLsJcELQkvybu3H4NSw2HoHC0yIoBV_lB2Tmha5KDB9fO9-lD2LcY0xp5UQT7OjggvKRUWOs18NWubb4BNYh_78RnFsR2fTKWqWZ-QUOehVslcw7FCAfhxUgojSClBz1kyVOJlHQMoZZIIf-1VCyQ8QlNOA2h0y_trlM9G6HjXpy7fPYqrshQzaqrS6Vjs0WTdBtdb4bbTxefakU0OEF_N5kv34-OH7-af88uvy4ry5zDUvRMqrUhUtCGJarStCai46rsnUqyptypoQ1rV1rbkQqsLUtNjQErg2plSsYiWjJ9nrg-528FHOzxklEbzCNS5LOiEuDgjj1Vpug92osJNeWXlT8KGXKiSrB5BYCWBKdAI0YZXCbWe47gqqCsKhFO2k9X52G9sNGA0uBTU8EH3YcXYle38lGSXTD4pJ4O0sEPzPEWKSGxs1DINy4MebuQvGSM320Df_QP-_HTugdPAxBujuhiFY7iN2y5L7iMk5YhPt1f1F7ki3maJ_AUTC0ZY</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Xu, Dong-bei</creator><creator>Chen, Ming</creator><creator>Ma, Ya-nan</creator><creator>Xu, Zhao-shi</creator><creator>Li, Lian-cheng</creator><creator>Chen, Yao-feng</creator><creator>Ma, You-zhi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>2015</creationdate><title>A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis</title><author>Xu, Dong-bei ; Chen, Ming ; Ma, Ya-nan ; Xu, Zhao-shi ; Li, Lian-cheng ; Chen, Yao-feng ; Ma, You-zhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-87a2be61dbcc811956f5c1c5288cd79114fb99c566a803db0d37e5cdd7a484743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Dong-bei</au><au>Chen, Ming</au><au>Ma, Ya-nan</au><au>Xu, Zhao-shi</au><au>Li, Lian-cheng</au><au>Chen, Yao-feng</au><au>Ma, You-zhi</au><au>Zhang, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015</date><risdate>2015</risdate><volume>10</volume><issue>1</issue><spage>e0116385</spage><epage>e0116385</epage><pages>e0116385-e0116385</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Heterotrimeric G-proteins are versatile regulators involved in diverse cellular processes in eukaryotes. In plants, the function of G-proteins is primarily associated with ABA signaling. However, the downstream effectors and the molecular mechanisms in the ABA pathway remain largely unknown. In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate drought tolerance in Arabidopsis. Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling. Our study found that transcript levels of three ABA responsive genes, AtMPK6, AtVIP1 and AtMYB44 (downstream gene of AtVIP1), were significantly up-regulated in agb1-2 lines after ABA or drought treatments. Other ABA-responsive and drought-inducible genes, such as RD29A (downstream gene of AtMYB44), were also up-regulated in agb1-2 lines. Furthermore, overexpression of AtVIP1 resulted in hypersensitivity to ABA at seed germination and seedling stages, and significantly enhanced drought tolerance in transgenic plants. These results suggest that AGB1 was involved in the ABA signaling pathway and drought tolerance in Arabidopsis through down-regulating the AtMPK6, AtVIP1 and AtMYB44 cascade.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25635681</pmid><doi>10.1371/journal.pone.0116385</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - physiology Agriculture Arabidopsis Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Biology Crop science Dehydration - metabolism Down-Regulation Drought Drought resistance Droughts Eukaryotes Gene Expression Gene Expression Regulation, Plant Genes Germination GTP-Binding Protein beta Subunits - physiology Hypersensitivity Kinases Laboratories Mitogen-Activated Protein Kinases - genetics Mitogen-Activated Protein Kinases - metabolism Molecular modelling Plant Growth Regulators - physiology Protein kinase Proteins Regulators Seed germination Seedlings Seeds Signal Transduction Signaling Transcription factors Transgenic plants Triticeae |
| title | A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis |
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