Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development
Chemical disinfection of oral biofilms often leaves biofilm structures intact. This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely wit...
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| Veröffentlicht in: | PloS one 2015-01, Vol.10 (1), p.e0116647-e0116647 |
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| creator | Ohsumi, Tatsuya Takenaka, Shoji Wakamatsu, Rika Sakaue, Yuuki Narisawa, Naoki Senpuku, Hidenobu Ohshima, Hayato Terao, Yutaka Okiji, Takashi |
| description | Chemical disinfection of oral biofilms often leaves biofilm structures intact. This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. It was concluded that disinfected biofilm structures favored secondary bacterial adhesion. |
| doi_str_mv | 10.1371/journal.pone.0116647 |
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This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. It was concluded that disinfected biofilm structures favored secondary bacterial adhesion.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0116647</identifier><identifier>PMID: 25635770</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion ; Alcohols ; Analysis ; Antibiotics ; Antigens ; Antimicrobial agents ; Bacteria ; Bacterial Adhesion ; Biofilms ; Biofilms - growth & development ; Cell culture ; Composite materials ; Confocal ; Confocal microscopy ; Correlation analysis ; Dentin - microbiology ; Dentistry ; Disinfectants ; Disinfection ; Disks ; Fluorescence ; Fluorides ; Infection control ; Infectious diseases ; Isopropanol ; Isopropyl alcohol ; Microorganisms ; Microscopic analysis ; Microscopy ; Oral hygiene ; Porphyromonas gingivalis ; Proteins ; Reactors ; Regression analysis ; Rotating disks ; Scanning microscopy ; Science ; Staphylococcus aureus ; Staphylococcus epidermidis ; Streptococcus ; Streptococcus infections ; Streptococcus mutans ; Streptococcus mutans - physiology ; Surface Properties ; University graduates</subject><ispartof>PloS one, 2015-01, Vol.10 (1), p.e0116647-e0116647</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ohsumi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Ohsumi et al 2015 Ohsumi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-e4ddede37340992536b45a01593a27f687e478c8f7aaa3f48f24082d427ad223</citedby><cites>FETCH-LOGICAL-c758t-e4ddede37340992536b45a01593a27f687e478c8f7aaa3f48f24082d427ad223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312048/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312048/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25635770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Xu, Ping</contributor><creatorcontrib>Ohsumi, Tatsuya</creatorcontrib><creatorcontrib>Takenaka, Shoji</creatorcontrib><creatorcontrib>Wakamatsu, Rika</creatorcontrib><creatorcontrib>Sakaue, Yuuki</creatorcontrib><creatorcontrib>Narisawa, Naoki</creatorcontrib><creatorcontrib>Senpuku, Hidenobu</creatorcontrib><creatorcontrib>Ohshima, Hayato</creatorcontrib><creatorcontrib>Terao, Yutaka</creatorcontrib><creatorcontrib>Okiji, Takashi</creatorcontrib><title>Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Chemical disinfection of oral biofilms often leaves biofilm structures intact. This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. 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This study aimed to examine whether the residual structure promotes secondary bacterial adhesion. Streptococcus mutans biofilms generated on resin-composite disks in a rotating disc reactor were disinfected completely with 70% isopropyl alcohol, and were again cultured in the same reactor after resupplying with the same bacterial solution. Specimens were subjected to fluorescence confocal laser scanning microscopy, viable cell counts and PCR-Invader assay in order to observe and quantify secondarily adhered cells. Fluorescence microscopic analysis, particularly after longitudinal cryosectioning, demonstrated stratified patterns of viable cells on the disinfected biofilm structure. Viable cell counts of test specimens were significantly higher than those of controls, and increased according to the amount of residual structure and culture period. Linear regression analysis exhibited a high correlation between viable and total cell counts. It was concluded that disinfected biofilm structures favored secondary bacterial adhesion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25635770</pmid><doi>10.1371/journal.pone.0116647</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesion Alcohols Analysis Antibiotics Antigens Antimicrobial agents Bacteria Bacterial Adhesion Biofilms Biofilms - growth & development Cell culture Composite materials Confocal Confocal microscopy Correlation analysis Dentin - microbiology Dentistry Disinfectants Disinfection Disks Fluorescence Fluorides Infection control Infectious diseases Isopropanol Isopropyl alcohol Microorganisms Microscopic analysis Microscopy Oral hygiene Porphyromonas gingivalis Proteins Reactors Regression analysis Rotating disks Scanning microscopy Science Staphylococcus aureus Staphylococcus epidermidis Streptococcus Streptococcus infections Streptococcus mutans Streptococcus mutans - physiology Surface Properties University graduates |
| title | Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development |
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