PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells
CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) w...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-02, Vol.10 (2), p.e0117484-e0117484 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0117484 |
|---|---|
| container_issue | 2 |
| container_start_page | e0117484 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Rodriguez, Juan M Marchicio, José López, Mariela Ziblat, Andrea Elias, Fernanda Fló, Juan López, Ricardo A Horn, David Zorzopulos, Jorge Montaner, Alejandro D |
| description | CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) with some of these ODNs led to secretion of significant amounts of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and granulocyte/monocyte colony-stimulating factor (GM-CSF), but only if interleukin 2 (IL2) was present. IMT504, the prototype of the PyNTTTTGT ODN class, was the most active. GM-CSF secretion was very efficient when non-CpG ODNs with high T content and PyNTTTTGT motifs lacking CpGs were used. On the other hand, CpG ODNs and IFNα inhibited this GM-CSF secretion. Selective cell type removal from hPBMC indicated that CD56+ cells were responsible for GM-CSF secretion and that plasmacytoid dendritic cells (PDCs) regulate this process. In addition, PyNTTTTGT ODNs inhibited the IFNα secretion induced by CpG ODNs in PDCs by interference with the TLR9 signaling pathway. Since IFNα is essential for CD56+ stimulation by CpG ODNs, there is a reciprocal interference of CpG and PyNTTTTGT ODNs when acting on this cell population. This suggests that these synthetic ODNs mimic different natural alarm signals for activation of the immune system. |
| doi_str_mv | 10.1371/journal.pone.0117484 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1658081985</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_84271835dc484c70bc6019e118fa8ee1</doaj_id><sourcerecordid>1658421846</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-4a8c36e0ce22c5a7e0902a7ab49eebefebec98a6caa94665716fa928fce6b80a3</originalsourceid><addsrcrecordid>eNptktFu0zAUhiMEYmPwBggscdMJpbPjxHF2gYQKK9NGQaJcWyfuSZcqsYudIOV5eFHcNp02hCXLlv2fz-cc_1H0mtEp4zm72NjeGWimW2twShnLU5k-iU5ZwZNYJJQ_fbA_iV54v6E041KI59FJkuVUFKk4jf58HxbLMOZLAmZFZts5qdu2N9Z3dds30Fk3ENvUa2t63aDt6hX6S4JVhboj1pC1A9M3Vg8dXrTW7DdE28aaIT4yarMmFejAIpP513j24-qceNQOuzoQyoHc9S0YMvuUifdksrjZp7K4WZ4TjU3jX0bPKmg8vhrXs-jn1efl7Et8-21-Pft4G-ssEV2cgtRcINWYJDqDHGlBE8ihTAvEEqswdSFBaIBQushyJiooEllpFKWkwM-itwfutrFejf31iolMUskKmQXF9UGxsrBRW1e34AZloVb7A-vWClxXh0YpmSY5kzxb6fAvOqelFpQVyJisQCKywPowvtaXLa40ms5B8wj6-MbUd2ptf6uUc8npDjAZAc7-6tF3qq39rmFg0PaHvNOEyVQE6bt_pP-vLj2otLPeO6zuk2FU7Tx3jFI7z6nRcyHszcNC7oOOJuN_AUW61wM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1658081985</pqid></control><display><type>article</type><title>PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Rodriguez, Juan M ; Marchicio, José ; López, Mariela ; Ziblat, Andrea ; Elias, Fernanda ; Fló, Juan ; López, Ricardo A ; Horn, David ; Zorzopulos, Jorge ; Montaner, Alejandro D</creator><contributor>Reeves, R. Keith</contributor><creatorcontrib>Rodriguez, Juan M ; Marchicio, José ; López, Mariela ; Ziblat, Andrea ; Elias, Fernanda ; Fló, Juan ; López, Ricardo A ; Horn, David ; Zorzopulos, Jorge ; Montaner, Alejandro D ; Reeves, R. Keith</creatorcontrib><description>CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) with some of these ODNs led to secretion of significant amounts of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and granulocyte/monocyte colony-stimulating factor (GM-CSF), but only if interleukin 2 (IL2) was present. IMT504, the prototype of the PyNTTTTGT ODN class, was the most active. GM-CSF secretion was very efficient when non-CpG ODNs with high T content and PyNTTTTGT motifs lacking CpGs were used. On the other hand, CpG ODNs and IFNα inhibited this GM-CSF secretion. Selective cell type removal from hPBMC indicated that CD56+ cells were responsible for GM-CSF secretion and that plasmacytoid dendritic cells (PDCs) regulate this process. In addition, PyNTTTTGT ODNs inhibited the IFNα secretion induced by CpG ODNs in PDCs by interference with the TLR9 signaling pathway. Since IFNα is essential for CD56+ stimulation by CpG ODNs, there is a reciprocal interference of CpG and PyNTTTTGT ODNs when acting on this cell population. This suggests that these synthetic ODNs mimic different natural alarm signals for activation of the immune system.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117484</identifier><identifier>PMID: 25706946</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>CD56 antigen ; Colonies ; Colony-stimulating factor ; CpG islands ; Cytokines ; Dendritic cells ; Granulocyte-macrophage colony-stimulating factor ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; Granulocytes ; Humans ; Immune system ; Immunostimulation ; Infections ; Inhibition ; Interference ; Interferon ; Interferon-alpha - metabolism ; Interferon-gamma - metabolism ; Interleukin ; Interleukin 2 ; Interleukin-2 - pharmacology ; Interleukin-8 - metabolism ; Killer Cells, Natural - drug effects ; Killer Cells, Natural - metabolism ; Leukocytes (granulocytic) ; Leukocytes (mononuclear) ; Lymphocytes ; Monocytes ; Natural Killer T-Cells - drug effects ; Natural Killer T-Cells - metabolism ; Oligodeoxyribonucleotides - pharmacology ; Oligonucleotides ; Peripheral blood mononuclear cells ; Signal transduction ; T cell receptors ; TLR9 protein ; Toll-like receptors ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-α ; γ-Interferon</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0117484-e0117484</ispartof><rights>2015 Rodriguez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Rodriguez et al 2015 Rodriguez et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-4a8c36e0ce22c5a7e0902a7ab49eebefebec98a6caa94665716fa928fce6b80a3</citedby><cites>FETCH-LOGICAL-c526t-4a8c36e0ce22c5a7e0902a7ab49eebefebec98a6caa94665716fa928fce6b80a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338301/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338301/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79357,79358</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25706946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Reeves, R. Keith</contributor><creatorcontrib>Rodriguez, Juan M</creatorcontrib><creatorcontrib>Marchicio, José</creatorcontrib><creatorcontrib>López, Mariela</creatorcontrib><creatorcontrib>Ziblat, Andrea</creatorcontrib><creatorcontrib>Elias, Fernanda</creatorcontrib><creatorcontrib>Fló, Juan</creatorcontrib><creatorcontrib>López, Ricardo A</creatorcontrib><creatorcontrib>Horn, David</creatorcontrib><creatorcontrib>Zorzopulos, Jorge</creatorcontrib><creatorcontrib>Montaner, Alejandro D</creatorcontrib><title>PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) with some of these ODNs led to secretion of significant amounts of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and granulocyte/monocyte colony-stimulating factor (GM-CSF), but only if interleukin 2 (IL2) was present. IMT504, the prototype of the PyNTTTTGT ODN class, was the most active. GM-CSF secretion was very efficient when non-CpG ODNs with high T content and PyNTTTTGT motifs lacking CpGs were used. On the other hand, CpG ODNs and IFNα inhibited this GM-CSF secretion. Selective cell type removal from hPBMC indicated that CD56+ cells were responsible for GM-CSF secretion and that plasmacytoid dendritic cells (PDCs) regulate this process. In addition, PyNTTTTGT ODNs inhibited the IFNα secretion induced by CpG ODNs in PDCs by interference with the TLR9 signaling pathway. Since IFNα is essential for CD56+ stimulation by CpG ODNs, there is a reciprocal interference of CpG and PyNTTTTGT ODNs when acting on this cell population. This suggests that these synthetic ODNs mimic different natural alarm signals for activation of the immune system.</description><subject>CD56 antigen</subject><subject>Colonies</subject><subject>Colony-stimulating factor</subject><subject>CpG islands</subject><subject>Cytokines</subject><subject>Dendritic cells</subject><subject>Granulocyte-macrophage colony-stimulating factor</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Granulocytes</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunostimulation</subject><subject>Infections</subject><subject>Inhibition</subject><subject>Interference</subject><subject>Interferon</subject><subject>Interferon-alpha - metabolism</subject><subject>Interferon-gamma - metabolism</subject><subject>Interleukin</subject><subject>Interleukin 2</subject><subject>Interleukin-2 - pharmacology</subject><subject>Interleukin-8 - metabolism</subject><subject>Killer Cells, Natural - drug effects</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Leukocytes (granulocytic)</subject><subject>Leukocytes (mononuclear)</subject><subject>Lymphocytes</subject><subject>Monocytes</subject><subject>Natural Killer T-Cells - drug effects</subject><subject>Natural Killer T-Cells - metabolism</subject><subject>Oligodeoxyribonucleotides - pharmacology</subject><subject>Oligonucleotides</subject><subject>Peripheral blood mononuclear cells</subject><subject>Signal transduction</subject><subject>T cell receptors</subject><subject>TLR9 protein</subject><subject>Toll-like receptors</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-α</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptktFu0zAUhiMEYmPwBggscdMJpbPjxHF2gYQKK9NGQaJcWyfuSZcqsYudIOV5eFHcNp02hCXLlv2fz-cc_1H0mtEp4zm72NjeGWimW2twShnLU5k-iU5ZwZNYJJQ_fbA_iV54v6E041KI59FJkuVUFKk4jf58HxbLMOZLAmZFZts5qdu2N9Z3dds30Fk3ENvUa2t63aDt6hX6S4JVhboj1pC1A9M3Vg8dXrTW7DdE28aaIT4yarMmFejAIpP513j24-qceNQOuzoQyoHc9S0YMvuUifdksrjZp7K4WZ4TjU3jX0bPKmg8vhrXs-jn1efl7Et8-21-Pft4G-ssEV2cgtRcINWYJDqDHGlBE8ihTAvEEqswdSFBaIBQushyJiooEllpFKWkwM-itwfutrFejf31iolMUskKmQXF9UGxsrBRW1e34AZloVb7A-vWClxXh0YpmSY5kzxb6fAvOqelFpQVyJisQCKywPowvtaXLa40ms5B8wj6-MbUd2ptf6uUc8npDjAZAc7-6tF3qq39rmFg0PaHvNOEyVQE6bt_pP-vLj2otLPeO6zuk2FU7Tx3jFI7z6nRcyHszcNC7oOOJuN_AUW61wM</recordid><startdate>20150223</startdate><enddate>20150223</enddate><creator>Rodriguez, Juan M</creator><creator>Marchicio, José</creator><creator>López, Mariela</creator><creator>Ziblat, Andrea</creator><creator>Elias, Fernanda</creator><creator>Fló, Juan</creator><creator>López, Ricardo A</creator><creator>Horn, David</creator><creator>Zorzopulos, Jorge</creator><creator>Montaner, Alejandro D</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150223</creationdate><title>PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells</title><author>Rodriguez, Juan M ; Marchicio, José ; López, Mariela ; Ziblat, Andrea ; Elias, Fernanda ; Fló, Juan ; López, Ricardo A ; Horn, David ; Zorzopulos, Jorge ; Montaner, Alejandro D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-4a8c36e0ce22c5a7e0902a7ab49eebefebec98a6caa94665716fa928fce6b80a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>CD56 antigen</topic><topic>Colonies</topic><topic>Colony-stimulating factor</topic><topic>CpG islands</topic><topic>Cytokines</topic><topic>Dendritic cells</topic><topic>Granulocyte-macrophage colony-stimulating factor</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Granulocytes</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunostimulation</topic><topic>Infections</topic><topic>Inhibition</topic><topic>Interference</topic><topic>Interferon</topic><topic>Interferon-alpha - metabolism</topic><topic>Interferon-gamma - metabolism</topic><topic>Interleukin</topic><topic>Interleukin 2</topic><topic>Interleukin-2 - pharmacology</topic><topic>Interleukin-8 - metabolism</topic><topic>Killer Cells, Natural - drug effects</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Leukocytes (granulocytic)</topic><topic>Leukocytes (mononuclear)</topic><topic>Lymphocytes</topic><topic>Monocytes</topic><topic>Natural Killer T-Cells - drug effects</topic><topic>Natural Killer T-Cells - metabolism</topic><topic>Oligodeoxyribonucleotides - pharmacology</topic><topic>Oligonucleotides</topic><topic>Peripheral blood mononuclear cells</topic><topic>Signal transduction</topic><topic>T cell receptors</topic><topic>TLR9 protein</topic><topic>Toll-like receptors</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Tumor necrosis factor-α</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez, Juan M</creatorcontrib><creatorcontrib>Marchicio, José</creatorcontrib><creatorcontrib>López, Mariela</creatorcontrib><creatorcontrib>Ziblat, Andrea</creatorcontrib><creatorcontrib>Elias, Fernanda</creatorcontrib><creatorcontrib>Fló, Juan</creatorcontrib><creatorcontrib>López, Ricardo A</creatorcontrib><creatorcontrib>Horn, David</creatorcontrib><creatorcontrib>Zorzopulos, Jorge</creatorcontrib><creatorcontrib>Montaner, Alejandro D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez, Juan M</au><au>Marchicio, José</au><au>López, Mariela</au><au>Ziblat, Andrea</au><au>Elias, Fernanda</au><au>Fló, Juan</au><au>López, Ricardo A</au><au>Horn, David</au><au>Zorzopulos, Jorge</au><au>Montaner, Alejandro D</au><au>Reeves, R. Keith</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-23</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0117484</spage><epage>e0117484</epage><pages>e0117484-e0117484</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>CD56+ cells have been recognized as being involved in bridging the innate and acquired immune systems. Herein, we assessed the effect of two major classes of immunostimulatory oligonucleotides (ODNs), PyNTTTTGT and CpG, on CD56+ cells. Incubation of human peripheral blood mononuclear cells (hPBMC) with some of these ODNs led to secretion of significant amounts of interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and granulocyte/monocyte colony-stimulating factor (GM-CSF), but only if interleukin 2 (IL2) was present. IMT504, the prototype of the PyNTTTTGT ODN class, was the most active. GM-CSF secretion was very efficient when non-CpG ODNs with high T content and PyNTTTTGT motifs lacking CpGs were used. On the other hand, CpG ODNs and IFNα inhibited this GM-CSF secretion. Selective cell type removal from hPBMC indicated that CD56+ cells were responsible for GM-CSF secretion and that plasmacytoid dendritic cells (PDCs) regulate this process. In addition, PyNTTTTGT ODNs inhibited the IFNα secretion induced by CpG ODNs in PDCs by interference with the TLR9 signaling pathway. Since IFNα is essential for CD56+ stimulation by CpG ODNs, there is a reciprocal interference of CpG and PyNTTTTGT ODNs when acting on this cell population. This suggests that these synthetic ODNs mimic different natural alarm signals for activation of the immune system.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25706946</pmid><doi>10.1371/journal.pone.0117484</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-02, Vol.10 (2), p.e0117484-e0117484 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1658081985 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | CD56 antigen Colonies Colony-stimulating factor CpG islands Cytokines Dendritic cells Granulocyte-macrophage colony-stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Granulocytes Humans Immune system Immunostimulation Infections Inhibition Interference Interferon Interferon-alpha - metabolism Interferon-gamma - metabolism Interleukin Interleukin 2 Interleukin-2 - pharmacology Interleukin-8 - metabolism Killer Cells, Natural - drug effects Killer Cells, Natural - metabolism Leukocytes (granulocytic) Leukocytes (mononuclear) Lymphocytes Monocytes Natural Killer T-Cells - drug effects Natural Killer T-Cells - metabolism Oligodeoxyribonucleotides - pharmacology Oligonucleotides Peripheral blood mononuclear cells Signal transduction T cell receptors TLR9 protein Toll-like receptors Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α γ-Interferon |
| title | PyNTTTTGT and CpG immunostimulatory oligonucleotides: effect on granulocyte/monocyte colony-stimulating factor (GM-CSF) secretion by human CD56+ (NK and NKT) cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T11%3A08%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PyNTTTTGT%20and%20CpG%20immunostimulatory%20oligonucleotides:%20effect%20on%20granulocyte/monocyte%20colony-stimulating%20factor%20(GM-CSF)%20secretion%20by%20human%20CD56+%20(NK%20and%20NKT)%20cells&rft.jtitle=PloS%20one&rft.au=Rodriguez,%20Juan%20M&rft.date=2015-02-23&rft.volume=10&rft.issue=2&rft.spage=e0117484&rft.epage=e0117484&rft.pages=e0117484-e0117484&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117484&rft_dat=%3Cproquest_plos_%3E1658421846%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1658081985&rft_id=info:pmid/25706946&rft_doaj_id=oai_doaj_org_article_84271835dc484c70bc6019e118fa8ee1&rfr_iscdi=true |