Associations among Sebox and other MEGs and its effects on early embryogenesis
In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the exp...
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description | In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs. |
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The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0115050</identifier><identifier>PMID: 25679966</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Animals ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Deoxyribonucleic acid ; Developmental stages ; DNA ; DNA methylation ; Egg Proteins - genetics ; Embryogenesis ; Embryonic Development ; Embryonic growth stage ; Embryos ; Female ; Gene expression ; Gene Expression Regulation, Developmental ; Gene Knockdown Techniques ; Genes ; Genetic Markers - genetics ; Genomes ; Genomics ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Laboratory animals ; Life sciences ; Male ; Metaphase ; Mice ; Mice, Inbred ICR ; Oocytes ; Pregnancy ; Ribonucleic acid ; RNA ; RNA Interference ; RNA Stability ; RNA, Messenger - chemistry ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA-mediated interference ; Transcription ; Transcription factors ; Transcription, Genetic ; Zygote - metabolism</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0115050-e0115050</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Park et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Park et al 2015 Park et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e76e49fbb78b2908c075a5bf65fcdd18c76bf3307a1c27758b3863158113fdb93</citedby><cites>FETCH-LOGICAL-c692t-e76e49fbb78b2908c075a5bf65fcdd18c76bf3307a1c27758b3863158113fdb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25679966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Qing-Yuan</contributor><creatorcontrib>Park, Min-Woo</creatorcontrib><creatorcontrib>Kim, Kyeoung-Hwa</creatorcontrib><creatorcontrib>Kim, Eun-Young</creatorcontrib><creatorcontrib>Lee, Su-Yeon</creatorcontrib><creatorcontrib>Ko, Jung-Jae</creatorcontrib><creatorcontrib>Lee, Kyung-Ah</creatorcontrib><title>Associations among Sebox and other MEGs and its effects on early embryogenesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.</description><subject>Activation</subject><subject>Animals</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental stages</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Egg Proteins - genetics</subject><subject>Embryogenesis</subject><subject>Embryonic Development</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Knockdown Techniques</subject><subject>Genes</subject><subject>Genetic Markers - genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Laboratory animals</subject><subject>Life sciences</subject><subject>Male</subject><subject>Metaphase</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Oocytes</subject><subject>Pregnancy</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA Stability</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-mediated interference</subject><subject>Transcription</subject><subject>Transcription factors</subject><subject>Transcription, Genetic</subject><subject>Zygote - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1v0zAUhiMEYqPwDxBEQkJw0WLHsZPcIFXTGJUGkxhwa9nOceopsTs7Qeu_x22zqUG7QJblr-e8xz5-k-Q1RgtMCvzpxg3einaxcRYWCGOKKHqSnOKKZHOWIfL0aH6SvAjhBiFKSsaeJycZZUVVMXaafF-G4JQRvXE2pKJztkmvQbq7VNg6df0afPrt_CLsl6YPKWgNKo7OpiB8u02hk37rGrAQTHiZPNOiDfBqHGfJry_nP8--zi-vLlZny8u5YlXWz6FgkFdayqKUWYVKhQoqqNSMalXXuFQFk5oQVAissqKgpYwXJ5iWGBNdy4rMkrcH3U3rAh9LEThmlLGqLFkWidWBqJ244RtvOuG33AnD9xvON1z43qgWOJU0rxnTSKM6l4rKXGEl81xprXPMSNT6PGYbZAe1Att70U5EpyfWrHnj_vCcEFwQFAU-jALe3Q4Qet6ZoKBthQU3HO6NcrLrs-TdP-jjrxupRsQHGKtdzKt2onyZZxnKGK52WotHqNhq6IyKvtEm7k8CPk4CItPDXd-IIQS-uv7x_-zV7yn7_ohdg2j7dXDtsHfdFMwPoPIuBA_6ocgY8Z3t76vBd7bno-1j2JvjD3oIuvc5-Qu_YPwc</recordid><startdate>20150213</startdate><enddate>20150213</enddate><creator>Park, Min-Woo</creator><creator>Kim, Kyeoung-Hwa</creator><creator>Kim, Eun-Young</creator><creator>Lee, Su-Yeon</creator><creator>Ko, Jung-Jae</creator><creator>Lee, Kyung-Ah</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150213</creationdate><title>Associations among Sebox and other MEGs and its effects on early embryogenesis</title><author>Park, Min-Woo ; Kim, Kyeoung-Hwa ; Kim, Eun-Young ; Lee, Su-Yeon ; Ko, Jung-Jae ; Lee, Kyung-Ah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-e76e49fbb78b2908c075a5bf65fcdd18c76bf3307a1c27758b3863158113fdb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Activation</topic><topic>Animals</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental stages</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>Egg Proteins - genetics</topic><topic>Embryogenesis</topic><topic>Embryonic Development</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Knockdown Techniques</topic><topic>Genes</topic><topic>Genetic Markers - genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Laboratory animals</topic><topic>Life sciences</topic><topic>Male</topic><topic>Metaphase</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Oocytes</topic><topic>Pregnancy</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA Stability</topic><topic>RNA, Messenger - chemistry</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-mediated interference</topic><topic>Transcription</topic><topic>Transcription factors</topic><topic>Transcription, Genetic</topic><topic>Zygote - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Min-Woo</creatorcontrib><creatorcontrib>Kim, Kyeoung-Hwa</creatorcontrib><creatorcontrib>Kim, Eun-Young</creatorcontrib><creatorcontrib>Lee, Su-Yeon</creatorcontrib><creatorcontrib>Ko, Jung-Jae</creatorcontrib><creatorcontrib>Lee, Kyung-Ah</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Min-Woo</au><au>Kim, Kyeoung-Hwa</au><au>Kim, Eun-Young</au><au>Lee, Su-Yeon</au><au>Ko, Jung-Jae</au><au>Lee, Kyung-Ah</au><au>Sun, Qing-Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Associations among Sebox and other MEGs and its effects on early embryogenesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-13</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0115050</spage><epage>e0115050</epage><pages>e0115050-e0115050</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25679966</pmid><doi>10.1371/journal.pone.0115050</doi><oa>free_for_read</oa></addata></record> |
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subjects | Activation Animals Basic Helix-Loop-Helix Transcription Factors - genetics Deoxyribonucleic acid Developmental stages DNA DNA methylation Egg Proteins - genetics Embryogenesis Embryonic Development Embryonic growth stage Embryos Female Gene expression Gene Expression Regulation, Developmental Gene Knockdown Techniques Genes Genetic Markers - genetics Genomes Genomics Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Laboratory animals Life sciences Male Metaphase Mice Mice, Inbred ICR Oocytes Pregnancy Ribonucleic acid RNA RNA Interference RNA Stability RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism RNA-mediated interference Transcription Transcription factors Transcription, Genetic Zygote - metabolism |
title | Associations among Sebox and other MEGs and its effects on early embryogenesis |
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