Associations among Sebox and other MEGs and its effects on early embryogenesis

In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the exp...

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Veröffentlicht in:PloS one 2015-02, Vol.10 (2), p.e0115050-e0115050
Hauptverfasser: Park, Min-Woo, Kim, Kyeoung-Hwa, Kim, Eun-Young, Lee, Su-Yeon, Ko, Jung-Jae, Lee, Kyung-Ah
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creator Park, Min-Woo
Kim, Kyeoung-Hwa
Kim, Eun-Young
Lee, Su-Yeon
Ko, Jung-Jae
Lee, Kyung-Ah
description In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. The present study was conducted to determine the mechanism of action for Sebox in this capacity, as shown by changes in the expression levels of other known MEG mRNAs after Sebox RNA interference (RNAi) in oocytes. Sebox-knockdown metaphase II (Mll) oocytes displayed normal morphology, but among the 23 MEGs monitored, 8 genes were upregulated, and 15 genes were unchanged. We hypothesized that the perturbed gene expression of these MEGs may cause the arrest of embryo development at the 2C stage and examined the expression of several marker genes for the degradation of maternal factors and zygotic genome activation. We found that some maternal mRNAs, c-mos, Gbx2, and Gdf9, were not fully degraded in Sebox-knockdown 2C embryos, and that several zygotic genome activation markers, Mt1a, Rpl23, Ube2a and Wee1, were not fully expressed in conjunction with diminished embryonic transcriptional activity. In addition, Sebox may be involved in the formation of the subcortical maternal complex through its regulation of the upstream regulator, Figla. Therefore, we concluded that Sebox is important in preparing oocytes for embryonic development by orchestrating the expression of other important MEGs.
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This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Park et al 2015 Park et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e76e49fbb78b2908c075a5bf65fcdd18c76bf3307a1c27758b3863158113fdb93</citedby><cites>FETCH-LOGICAL-c692t-e76e49fbb78b2908c075a5bf65fcdd18c76bf3307a1c27758b3863158113fdb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4331730/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25679966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sun, Qing-Yuan</contributor><creatorcontrib>Park, Min-Woo</creatorcontrib><creatorcontrib>Kim, Kyeoung-Hwa</creatorcontrib><creatorcontrib>Kim, Eun-Young</creatorcontrib><creatorcontrib>Lee, Su-Yeon</creatorcontrib><creatorcontrib>Ko, Jung-Jae</creatorcontrib><creatorcontrib>Lee, Kyung-Ah</creatorcontrib><title>Associations among Sebox and other MEGs and its effects on early embryogenesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In a previous report, we identified Sebox as a new candidate maternal effect gene that is essential for embryonic development and primarily impacts the two-cell (2C) stage. 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subjects Activation
Animals
Basic Helix-Loop-Helix Transcription Factors - genetics
Deoxyribonucleic acid
Developmental stages
DNA
DNA methylation
Egg Proteins - genetics
Embryogenesis
Embryonic Development
Embryonic growth stage
Embryos
Female
Gene expression
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Genes
Genetic Markers - genetics
Genomes
Genomics
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Laboratory animals
Life sciences
Male
Metaphase
Mice
Mice, Inbred ICR
Oocytes
Pregnancy
Ribonucleic acid
RNA
RNA Interference
RNA Stability
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-mediated interference
Transcription
Transcription factors
Transcription, Genetic
Zygote - metabolism
title Associations among Sebox and other MEGs and its effects on early embryogenesis
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