The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm

It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons....

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2015-02, Vol.10 (2), p.e0117546
Hauptverfasser: Yin, Liang, Xie, Yingying, Yin, Songyue, Lv, Xiaolei, Zhang, Jia, Gu, Zezong, Sun, Haidan, Liu, Siqi
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 2
container_start_page e0117546
container_title PloS one
container_volume 10
creator Yin, Liang
Xie, Yingying
Yin, Songyue
Lv, Xiaolei
Zhang, Jia
Gu, Zezong
Sun, Haidan
Liu, Siqi
description It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons. In this study, SH-SY5Y cells treated with rotenone were used as a model of neuronal death in PD. The treated cells underwent significant apoptosis, which was accompanied by an increase in intracellular NO in a rotenone dose-dependent manner. The CyDye switch approach was employed to screen for changes in S-nitrosylated (SNO) proteins in response to the rotenone treatment. Seven proteins with increased S-nitrosylation were identified in the treated SH-SY5Y cells, which included proliferating cell nuclear antigen (PCNA). Although PCNA is generally located in the nucleus and participates in DNA replication and repair, significant PCNA was identified in the SH-SY5Y cytosol. Using immunoprecipitation and pull-down approaches, PCNA was found to interact with caspase-9; using mass spectrometry, the two cysteine residues PCNA-Cys81 and -Cys162 were identified as candidate S-nitrosylated residues. In addition, the evidence obtained from in vitro and the cell model studies indicated that the S-nitrosylation of PCNA-Cys81 affected the interaction between PCNA and caspase-9. Furthermore, the interaction of PCNA and caspase-9 partially blocked caspase-9 activation, indicating that the S-nitrosylation of cytosolic PCNA may be a mediator of the apoptotic pathway.
doi_str_mv 10.1371/journal.pone.0117546
format Article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1654934469</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A425892442</galeid><doaj_id>oai_doaj_org_article_741f202b00e34d3f859c69e0222bec63</doaj_id><sourcerecordid>A425892442</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-164c0e28c994c26c5a7a4123db049418d05794ebcbf699247c256bbaffee96773</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBYmsTHRYvtOE59g1RVfFSa2MQGt9aJ47QuThxih1F-Pe6aTQ3aBcqFI_t5X59zfE6SPCd4StKcvNu4vmvATlvX6CkmJM8Yf5AcE5HSCac4fXjwf5Q88X6DcZbOOH-cHNGM5xkW-XFyfbXW6HLSmNA5v7UQjGuQDxB6j1yFLhZf5sg6Bdb80SUyDVLb4LyzyNQtqOBRiHpoXRtcMAq1ENbXsN2BgC6g-2Ea75rXHpXGa_A6Ah2UZlU_TR5VYL1-NqwnybePH64Wnydn55-Wi_nZRHFBw4RwprCmMyUEU5SrDHJghKZlgZlgZFbiLBdMF6qouBCU5SqmVhRQVVoLnufpSfJy79ta5-VQMy8Jz5hIGeMiEss9UTrYyLYzNXRb6cDImw3XrSR0MTerZc5IRTEtMNYpK9NqlokYpsaU0kIrnkav98NtfVHrUukmdGBHpuOTxqzlyv2SLKWcZbtg3gwGnfvZax9kbbzS1kKjXX8TN8csoiyip_-g92c3UCuICZimcvFetTOVc0azWawZo5Ga3kPFr9S1UbHBKhP3R4K3I0Fkgv4dVtB7L5eXX_-fPf8-Zl8dsGsNNqxjt_W7tvRjkO1BFfvWd7q6KzLBcjcft9WQu_mQw3xE2YvDB7oT3Q5E-hfBrgrm</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1654934469</pqid></control><display><type>article</type><title>The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Yin, Liang ; Xie, Yingying ; Yin, Songyue ; Lv, Xiaolei ; Zhang, Jia ; Gu, Zezong ; Sun, Haidan ; Liu, Siqi</creator><contributor>Ariga, Hiroyoshi</contributor><creatorcontrib>Yin, Liang ; Xie, Yingying ; Yin, Songyue ; Lv, Xiaolei ; Zhang, Jia ; Gu, Zezong ; Sun, Haidan ; Liu, Siqi ; Ariga, Hiroyoshi</creatorcontrib><description>It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons. In this study, SH-SY5Y cells treated with rotenone were used as a model of neuronal death in PD. The treated cells underwent significant apoptosis, which was accompanied by an increase in intracellular NO in a rotenone dose-dependent manner. The CyDye switch approach was employed to screen for changes in S-nitrosylated (SNO) proteins in response to the rotenone treatment. Seven proteins with increased S-nitrosylation were identified in the treated SH-SY5Y cells, which included proliferating cell nuclear antigen (PCNA). Although PCNA is generally located in the nucleus and participates in DNA replication and repair, significant PCNA was identified in the SH-SY5Y cytosol. Using immunoprecipitation and pull-down approaches, PCNA was found to interact with caspase-9; using mass spectrometry, the two cysteine residues PCNA-Cys81 and -Cys162 were identified as candidate S-nitrosylated residues. In addition, the evidence obtained from in vitro and the cell model studies indicated that the S-nitrosylation of PCNA-Cys81 affected the interaction between PCNA and caspase-9. Furthermore, the interaction of PCNA and caspase-9 partially blocked caspase-9 activation, indicating that the S-nitrosylation of cytosolic PCNA may be a mediator of the apoptotic pathway.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117546</identifier><identifier>PMID: 25675097</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Apoptosis ; Caspase ; Caspase 9 - metabolism ; Caspase-9 ; Cell Line, Tumor ; Cysteine ; Cytosol ; Cytosol - metabolism ; Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; DNA repair ; DNA replication ; Enzyme Activation ; Humans ; Immunoprecipitation ; Kinases ; Mass spectrometry ; Mass spectroscopy ; Models, Molecular ; Movement disorders ; Neurodegeneration ; Neurodegenerative diseases ; Neurophysiology ; Nitric oxide ; Nitric Oxide - metabolism ; Nuclei (cytology) ; Oxidative Stress - drug effects ; Parkinson Disease - metabolism ; Parkinson's disease ; Proliferating cell nuclear antigen ; Proliferating Cell Nuclear Antigen - chemistry ; Proliferating Cell Nuclear Antigen - metabolism ; Protein Binding ; Protein Conformation ; Protein S ; Protein Transport ; Proteins ; Proteome ; Proteomics - methods ; Reactive nitrogen species ; Residues ; Rodents ; Rotenone ; Rotenone - pharmacology ; Signal Transduction</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0117546</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Yin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Yin et al 2015 Yin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-164c0e28c994c26c5a7a4123db049418d05794ebcbf699247c256bbaffee96773</citedby><cites>FETCH-LOGICAL-c692t-164c0e28c994c26c5a7a4123db049418d05794ebcbf699247c256bbaffee96773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326459/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4326459/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25675097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ariga, Hiroyoshi</contributor><creatorcontrib>Yin, Liang</creatorcontrib><creatorcontrib>Xie, Yingying</creatorcontrib><creatorcontrib>Yin, Songyue</creatorcontrib><creatorcontrib>Lv, Xiaolei</creatorcontrib><creatorcontrib>Zhang, Jia</creatorcontrib><creatorcontrib>Gu, Zezong</creatorcontrib><creatorcontrib>Sun, Haidan</creatorcontrib><creatorcontrib>Liu, Siqi</creatorcontrib><title>The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons. In this study, SH-SY5Y cells treated with rotenone were used as a model of neuronal death in PD. The treated cells underwent significant apoptosis, which was accompanied by an increase in intracellular NO in a rotenone dose-dependent manner. The CyDye switch approach was employed to screen for changes in S-nitrosylated (SNO) proteins in response to the rotenone treatment. Seven proteins with increased S-nitrosylation were identified in the treated SH-SY5Y cells, which included proliferating cell nuclear antigen (PCNA). Although PCNA is generally located in the nucleus and participates in DNA replication and repair, significant PCNA was identified in the SH-SY5Y cytosol. Using immunoprecipitation and pull-down approaches, PCNA was found to interact with caspase-9; using mass spectrometry, the two cysteine residues PCNA-Cys81 and -Cys162 were identified as candidate S-nitrosylated residues. In addition, the evidence obtained from in vitro and the cell model studies indicated that the S-nitrosylation of PCNA-Cys81 affected the interaction between PCNA and caspase-9. Furthermore, the interaction of PCNA and caspase-9 partially blocked caspase-9 activation, indicating that the S-nitrosylation of cytosolic PCNA may be a mediator of the apoptotic pathway.</description><subject>Analysis</subject><subject>Apoptosis</subject><subject>Caspase</subject><subject>Caspase 9 - metabolism</subject><subject>Caspase-9</subject><subject>Cell Line, Tumor</subject><subject>Cysteine</subject><subject>Cytosol</subject><subject>Cytosol - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA repair</subject><subject>DNA replication</subject><subject>Enzyme Activation</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Kinases</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Models, Molecular</subject><subject>Movement disorders</subject><subject>Neurodegeneration</subject><subject>Neurodegenerative diseases</subject><subject>Neurophysiology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Nuclei (cytology)</subject><subject>Oxidative Stress - drug effects</subject><subject>Parkinson Disease - metabolism</subject><subject>Parkinson's disease</subject><subject>Proliferating cell nuclear antigen</subject><subject>Proliferating Cell Nuclear Antigen - chemistry</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>Protein Binding</subject><subject>Protein Conformation</subject><subject>Protein S</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteomics - methods</subject><subject>Reactive nitrogen species</subject><subject>Residues</subject><subject>Rodents</subject><subject>Rotenone</subject><subject>Rotenone - pharmacology</subject><subject>Signal Transduction</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBYmsTHRYvtOE59g1RVfFSa2MQGt9aJ47QuThxih1F-Pe6aTQ3aBcqFI_t5X59zfE6SPCd4StKcvNu4vmvATlvX6CkmJM8Yf5AcE5HSCac4fXjwf5Q88X6DcZbOOH-cHNGM5xkW-XFyfbXW6HLSmNA5v7UQjGuQDxB6j1yFLhZf5sg6Bdb80SUyDVLb4LyzyNQtqOBRiHpoXRtcMAq1ENbXsN2BgC6g-2Ea75rXHpXGa_A6Ah2UZlU_TR5VYL1-NqwnybePH64Wnydn55-Wi_nZRHFBw4RwprCmMyUEU5SrDHJghKZlgZlgZFbiLBdMF6qouBCU5SqmVhRQVVoLnufpSfJy79ta5-VQMy8Jz5hIGeMiEss9UTrYyLYzNXRb6cDImw3XrSR0MTerZc5IRTEtMNYpK9NqlokYpsaU0kIrnkav98NtfVHrUukmdGBHpuOTxqzlyv2SLKWcZbtg3gwGnfvZax9kbbzS1kKjXX8TN8csoiyip_-g92c3UCuICZimcvFetTOVc0azWawZo5Ga3kPFr9S1UbHBKhP3R4K3I0Fkgv4dVtB7L5eXX_-fPf8-Zl8dsGsNNqxjt_W7tvRjkO1BFfvWd7q6KzLBcjcft9WQu_mQw3xE2YvDB7oT3Q5E-hfBrgrm</recordid><startdate>20150212</startdate><enddate>20150212</enddate><creator>Yin, Liang</creator><creator>Xie, Yingying</creator><creator>Yin, Songyue</creator><creator>Lv, Xiaolei</creator><creator>Zhang, Jia</creator><creator>Gu, Zezong</creator><creator>Sun, Haidan</creator><creator>Liu, Siqi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150212</creationdate><title>The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm</title><author>Yin, Liang ; Xie, Yingying ; Yin, Songyue ; Lv, Xiaolei ; Zhang, Jia ; Gu, Zezong ; Sun, Haidan ; Liu, Siqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-164c0e28c994c26c5a7a4123db049418d05794ebcbf699247c256bbaffee96773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Apoptosis</topic><topic>Caspase</topic><topic>Caspase 9 - metabolism</topic><topic>Caspase-9</topic><topic>Cell Line, Tumor</topic><topic>Cysteine</topic><topic>Cytosol</topic><topic>Cytosol - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA repair</topic><topic>DNA replication</topic><topic>Enzyme Activation</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Kinases</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Models, Molecular</topic><topic>Movement disorders</topic><topic>Neurodegeneration</topic><topic>Neurodegenerative diseases</topic><topic>Neurophysiology</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Nuclei (cytology)</topic><topic>Oxidative Stress - drug effects</topic><topic>Parkinson Disease - metabolism</topic><topic>Parkinson's disease</topic><topic>Proliferating cell nuclear antigen</topic><topic>Proliferating Cell Nuclear Antigen - chemistry</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>Protein Binding</topic><topic>Protein Conformation</topic><topic>Protein S</topic><topic>Protein Transport</topic><topic>Proteins</topic><topic>Proteome</topic><topic>Proteomics - methods</topic><topic>Reactive nitrogen species</topic><topic>Residues</topic><topic>Rodents</topic><topic>Rotenone</topic><topic>Rotenone - pharmacology</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Liang</creatorcontrib><creatorcontrib>Xie, Yingying</creatorcontrib><creatorcontrib>Yin, Songyue</creatorcontrib><creatorcontrib>Lv, Xiaolei</creatorcontrib><creatorcontrib>Zhang, Jia</creatorcontrib><creatorcontrib>Gu, Zezong</creatorcontrib><creatorcontrib>Sun, Haidan</creatorcontrib><creatorcontrib>Liu, Siqi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Liang</au><au>Xie, Yingying</au><au>Yin, Songyue</au><au>Lv, Xiaolei</au><au>Zhang, Jia</au><au>Gu, Zezong</au><au>Sun, Haidan</au><au>Liu, Siqi</au><au>Ariga, Hiroyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-12</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0117546</spage><pages>e0117546-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>It is generally accepted that nitric oxide (NO) or its derivatives, reactive nitrogen species (RNS), are involved in the development of Parkinson's disease (PD). Recently, emerging evidence in the study of PD has indicated that protein S-nitrosylation triggers the signaling changes in neurons. In this study, SH-SY5Y cells treated with rotenone were used as a model of neuronal death in PD. The treated cells underwent significant apoptosis, which was accompanied by an increase in intracellular NO in a rotenone dose-dependent manner. The CyDye switch approach was employed to screen for changes in S-nitrosylated (SNO) proteins in response to the rotenone treatment. Seven proteins with increased S-nitrosylation were identified in the treated SH-SY5Y cells, which included proliferating cell nuclear antigen (PCNA). Although PCNA is generally located in the nucleus and participates in DNA replication and repair, significant PCNA was identified in the SH-SY5Y cytosol. Using immunoprecipitation and pull-down approaches, PCNA was found to interact with caspase-9; using mass spectrometry, the two cysteine residues PCNA-Cys81 and -Cys162 were identified as candidate S-nitrosylated residues. In addition, the evidence obtained from in vitro and the cell model studies indicated that the S-nitrosylation of PCNA-Cys81 affected the interaction between PCNA and caspase-9. Furthermore, the interaction of PCNA and caspase-9 partially blocked caspase-9 activation, indicating that the S-nitrosylation of cytosolic PCNA may be a mediator of the apoptotic pathway.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25675097</pmid><doi>10.1371/journal.pone.0117546</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-02, Vol.10 (2), p.e0117546
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1654934469
source MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry
subjects Analysis
Apoptosis
Caspase
Caspase 9 - metabolism
Caspase-9
Cell Line, Tumor
Cysteine
Cytosol
Cytosol - metabolism
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA repair
DNA replication
Enzyme Activation
Humans
Immunoprecipitation
Kinases
Mass spectrometry
Mass spectroscopy
Models, Molecular
Movement disorders
Neurodegeneration
Neurodegenerative diseases
Neurophysiology
Nitric oxide
Nitric Oxide - metabolism
Nuclei (cytology)
Oxidative Stress - drug effects
Parkinson Disease - metabolism
Parkinson's disease
Proliferating cell nuclear antigen
Proliferating Cell Nuclear Antigen - chemistry
Proliferating Cell Nuclear Antigen - metabolism
Protein Binding
Protein Conformation
Protein S
Protein Transport
Proteins
Proteome
Proteomics - methods
Reactive nitrogen species
Residues
Rodents
Rotenone
Rotenone - pharmacology
Signal Transduction
title The S-nitrosylation status of PCNA localized in cytosol impacts the apoptotic pathway in a Parkinson's disease paradigm
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T19%3A01%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20S-nitrosylation%20status%20of%20PCNA%20localized%20in%20cytosol%20impacts%20the%20apoptotic%20pathway%20in%20a%20Parkinson's%20disease%20paradigm&rft.jtitle=PloS%20one&rft.au=Yin,%20Liang&rft.date=2015-02-12&rft.volume=10&rft.issue=2&rft.spage=e0117546&rft.pages=e0117546-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117546&rft_dat=%3Cgale_plos_%3EA425892442%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1654934469&rft_id=info:pmid/25675097&rft_galeid=A425892442&rft_doaj_id=oai_doaj_org_article_741f202b00e34d3f859c69e0222bec63&rfr_iscdi=true