Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves
Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold...
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| creator | Tian, Chang Jiang, Qian Wang, Feng Wang, Guang-Long Xu, Zhi-Sheng Xiong, Ai-Sheng |
| description | Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold, salt, and drought) and hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid). The expression profiles of the candidate reference genes were evaluated in three technical and biological replicates. Real-time qPCR data analyses were performed using three commonly used Excel-based applets namely, BestKeeper, geNorm, and NormFinder. ACTIN and TUB were the most stable genes identified among all sample groups, but individual analysis revealed changes in their expression profiles. GAPDH displayed the maximum stability for most of single stresses. To further validate the suitability of the reference genes identified in this study, the expression profile of DcDREB-A1 gene (homolog of AtDREB-A1 gene of Arabidophsis) was studied in carrot. The appropriate reference genes were selected that showed stable expression under the different experimental conditions. |
| doi_str_mv | 10.1371/journal.pone.0117569 |
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In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold, salt, and drought) and hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid). The expression profiles of the candidate reference genes were evaluated in three technical and biological replicates. Real-time qPCR data analyses were performed using three commonly used Excel-based applets namely, BestKeeper, geNorm, and NormFinder. ACTIN and TUB were the most stable genes identified among all sample groups, but individual analysis revealed changes in their expression profiles. GAPDH displayed the maximum stability for most of single stresses. To further validate the suitability of the reference genes identified in this study, the expression profile of DcDREB-A1 gene (homolog of AtDREB-A1 gene of Arabidophsis) was studied in carrot. The appropriate reference genes were selected that showed stable expression under the different experimental conditions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117569</identifier><identifier>PMID: 25658122</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>A1 gene ; Abiotic stress ; Abscisic acid ; Abscisic Acid - pharmacology ; Acetates - pharmacology ; Acids ; Actin ; Antioxidants ; Apiaceae ; Carrots ; Cold ; Cold Temperature ; Cyclopentanes - pharmacology ; Daucus ; Daucus carota - drug effects ; Daucus carota - genetics ; Drought ; Droughts ; Gene expression ; Gene Expression Regulation, Plant - drug effects ; Gene Expression Regulation, Plant - physiology ; Genes ; Genes, Essential ; Genes, Plant ; Genomes ; Germplasm ; Gibberellins - pharmacology ; Glyceraldehyde-3-phosphate dehydrogenase ; Heat ; Homology ; Horticulture ; Hot Temperature ; Jasmonic acid ; Laboratories ; Leaves ; Methyl jasmonate ; Molecular biology ; Muscle proteins ; Oxylipins - pharmacology ; Plant Growth Regulators - pharmacology ; Plant Leaves - drug effects ; Plant Leaves - physiology ; Plant sciences ; Polymerase chain reaction ; Proteins ; Real-Time Polymerase Chain Reaction - standards ; Salicylic acid ; Salicylic Acid - pharmacology ; Salinity ; Signal transduction ; Sodium Chloride - pharmacology ; Soybeans ; Spreadsheet software ; Stimuli ; Stress, Physiological - genetics ; Stresses ; Studies ; Transcription factors ; Vegetables</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0117569-e0117569</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Tian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Tian et al 2015 Tian et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b158edb6e9dc66e07911e398c77a5e587da6c8a248ae6b0bfdb376307e2834913</citedby><cites>FETCH-LOGICAL-c692t-b158edb6e9dc66e07911e398c77a5e587da6c8a248ae6b0bfdb376307e2834913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319972/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319972/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25658122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Jain, Mukesh</contributor><creatorcontrib>Tian, Chang</creatorcontrib><creatorcontrib>Jiang, Qian</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Wang, Guang-Long</creatorcontrib><creatorcontrib>Xu, Zhi-Sheng</creatorcontrib><creatorcontrib>Xiong, Ai-Sheng</creatorcontrib><title>Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold, salt, and drought) and hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid). The expression profiles of the candidate reference genes were evaluated in three technical and biological replicates. Real-time qPCR data analyses were performed using three commonly used Excel-based applets namely, BestKeeper, geNorm, and NormFinder. ACTIN and TUB were the most stable genes identified among all sample groups, but individual analysis revealed changes in their expression profiles. GAPDH displayed the maximum stability for most of single stresses. To further validate the suitability of the reference genes identified in this study, the expression profile of DcDREB-A1 gene (homolog of AtDREB-A1 gene of Arabidophsis) was studied in carrot. The appropriate reference genes were selected that showed stable expression under the different experimental conditions.</description><subject>A1 gene</subject><subject>Abiotic stress</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Acetates - pharmacology</subject><subject>Acids</subject><subject>Actin</subject><subject>Antioxidants</subject><subject>Apiaceae</subject><subject>Carrots</subject><subject>Cold</subject><subject>Cold Temperature</subject><subject>Cyclopentanes - pharmacology</subject><subject>Daucus</subject><subject>Daucus carota - drug effects</subject><subject>Daucus carota - genetics</subject><subject>Drought</subject><subject>Droughts</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genes</subject><subject>Genes, Essential</subject><subject>Genes, Plant</subject><subject>Genomes</subject><subject>Germplasm</subject><subject>Gibberellins - pharmacology</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Heat</subject><subject>Homology</subject><subject>Horticulture</subject><subject>Hot Temperature</subject><subject>Jasmonic acid</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Methyl jasmonate</subject><subject>Molecular biology</subject><subject>Muscle proteins</subject><subject>Oxylipins - pharmacology</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - physiology</subject><subject>Plant sciences</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction - standards</subject><subject>Salicylic acid</subject><subject>Salicylic Acid - pharmacology</subject><subject>Salinity</subject><subject>Signal transduction</subject><subject>Sodium Chloride - pharmacology</subject><subject>Soybeans</subject><subject>Spreadsheet software</subject><subject>Stimuli</subject><subject>Stress, Physiological - genetics</subject><subject>Stresses</subject><subject>Studies</subject><subject>Transcription factors</subject><subject>Vegetables</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIHqxaz5mkslNoRQ_FgqVVr0NmcyZ3SzZZJvMlOqvN7s7LbvSC8lFwsnzvsk5ySmK1wRPCRPk0zIM0Ws3XQcPU0yIqLh8UhwTyeiEU8ye7q2PihcpLTGuWM358-KIVryqCaXHxd01ODC9DR6FDqXB9rpxgCJ0EMEbQHPwkFAXIrr5fn6FfIgr7ewfvZUMvoWIdGNDbw1KfYSUMq19ixYZzDfLQbsanEXWI6NjDD1yoG8hvSyeddoleDXOJ8XPL59_nH-bXFx-nZ2fXUwMl7SfNKSqoW04yNZwDlhIQoDJ2gihK6hq0Wpuak3LWgNvcNO1DROcYQG0ZqUk7KR4u_Ndu5DUWLSkCK8okSJXJBOzHdEGvVTraFc6_lZBW7UNhDhXOub8HCgpu6qrTCexaMuSMM1rg2VXUlrhsmo2XqfjaUOzgtaA76N2B6aHO94u1DzcqpIRKQXNBh9GgxhuBki9WtlkwDntIQzbezNCBWM4o-_-QR_PbqTmOidgfRfyuWZjqs5KKmsiSiYzNX2EyqOFlTX5HTub4weCjweCzPRw18_1kJKaXV_9P3v565B9v8cuQLt-kYIbNt8tHYLlDjQxpJT_60ORCVabBrmvhto0iBobJMve7D_Qg-i-I9hfZOoMGg</recordid><startdate>20150206</startdate><enddate>20150206</enddate><creator>Tian, Chang</creator><creator>Jiang, Qian</creator><creator>Wang, Feng</creator><creator>Wang, Guang-Long</creator><creator>Xu, Zhi-Sheng</creator><creator>Xiong, Ai-Sheng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150206</creationdate><title>Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves</title><author>Tian, Chang ; Jiang, Qian ; Wang, Feng ; Wang, Guang-Long ; Xu, Zhi-Sheng ; Xiong, Ai-Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b158edb6e9dc66e07911e398c77a5e587da6c8a248ae6b0bfdb376307e2834913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>A1 gene</topic><topic>Abiotic stress</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Acetates - pharmacology</topic><topic>Acids</topic><topic>Actin</topic><topic>Antioxidants</topic><topic>Apiaceae</topic><topic>Carrots</topic><topic>Cold</topic><topic>Cold Temperature</topic><topic>Cyclopentanes - pharmacology</topic><topic>Daucus</topic><topic>Daucus carota - drug effects</topic><topic>Daucus carota - genetics</topic><topic>Drought</topic><topic>Droughts</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genes</topic><topic>Genes, Essential</topic><topic>Genes, Plant</topic><topic>Genomes</topic><topic>Germplasm</topic><topic>Gibberellins - pharmacology</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Heat</topic><topic>Homology</topic><topic>Horticulture</topic><topic>Hot Temperature</topic><topic>Jasmonic acid</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Methyl jasmonate</topic><topic>Molecular biology</topic><topic>Muscle proteins</topic><topic>Oxylipins - pharmacology</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - physiology</topic><topic>Plant sciences</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction - standards</topic><topic>Salicylic acid</topic><topic>Salicylic Acid - pharmacology</topic><topic>Salinity</topic><topic>Signal transduction</topic><topic>Sodium Chloride - pharmacology</topic><topic>Soybeans</topic><topic>Spreadsheet software</topic><topic>Stimuli</topic><topic>Stress, Physiological - genetics</topic><topic>Stresses</topic><topic>Studies</topic><topic>Transcription factors</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Chang</creatorcontrib><creatorcontrib>Jiang, Qian</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Wang, Guang-Long</creatorcontrib><creatorcontrib>Xu, Zhi-Sheng</creatorcontrib><creatorcontrib>Xiong, Ai-Sheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Chang</au><au>Jiang, Qian</au><au>Wang, Feng</au><au>Wang, Guang-Long</au><au>Xu, Zhi-Sheng</au><au>Xiong, Ai-Sheng</au><au>Jain, Mukesh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-06</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0117569</spage><epage>e0117569</epage><pages>e0117569-e0117569</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold, salt, and drought) and hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid). The expression profiles of the candidate reference genes were evaluated in three technical and biological replicates. Real-time qPCR data analyses were performed using three commonly used Excel-based applets namely, BestKeeper, geNorm, and NormFinder. ACTIN and TUB were the most stable genes identified among all sample groups, but individual analysis revealed changes in their expression profiles. GAPDH displayed the maximum stability for most of single stresses. To further validate the suitability of the reference genes identified in this study, the expression profile of DcDREB-A1 gene (homolog of AtDREB-A1 gene of Arabidophsis) was studied in carrot. The appropriate reference genes were selected that showed stable expression under the different experimental conditions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25658122</pmid><doi>10.1371/journal.pone.0117569</doi><tpages>e0117569</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1652197053 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | A1 gene Abiotic stress Abscisic acid Abscisic Acid - pharmacology Acetates - pharmacology Acids Actin Antioxidants Apiaceae Carrots Cold Cold Temperature Cyclopentanes - pharmacology Daucus Daucus carota - drug effects Daucus carota - genetics Drought Droughts Gene expression Gene Expression Regulation, Plant - drug effects Gene Expression Regulation, Plant - physiology Genes Genes, Essential Genes, Plant Genomes Germplasm Gibberellins - pharmacology Glyceraldehyde-3-phosphate dehydrogenase Heat Homology Horticulture Hot Temperature Jasmonic acid Laboratories Leaves Methyl jasmonate Molecular biology Muscle proteins Oxylipins - pharmacology Plant Growth Regulators - pharmacology Plant Leaves - drug effects Plant Leaves - physiology Plant sciences Polymerase chain reaction Proteins Real-Time Polymerase Chain Reaction - standards Salicylic acid Salicylic Acid - pharmacology Salinity Signal transduction Sodium Chloride - pharmacology Soybeans Spreadsheet software Stimuli Stress, Physiological - genetics Stresses Studies Transcription factors Vegetables |
| title | Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T15%3A33%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Selection%20of%20suitable%20reference%20genes%20for%20qPCR%20normalization%20under%20abiotic%20stresses%20and%20hormone%20stimuli%20in%20carrot%20leaves&rft.jtitle=PloS%20one&rft.au=Tian,%20Chang&rft.date=2015-02-06&rft.volume=10&rft.issue=2&rft.spage=e0117569&rft.epage=e0117569&rft.pages=e0117569-e0117569&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117569&rft_dat=%3Cgale_plos_%3EA429817439%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1652197053&rft_id=info:pmid/25658122&rft_galeid=A429817439&rft_doaj_id=oai_doaj_org_article_99f5f5cf907d4413a68c09f4225045b3&rfr_iscdi=true |