Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice
Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and t...
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| creator | Købler, Carsten Poulsen, Sarah S Saber, Anne T Jacobsen, Nicklas R Wallin, Håkan Yauk, Carole L Halappanavar, Sabina Vogel, Ulla Qvortrup, Klaus Mølhave, Kristian |
| description | Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.
TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.
Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP). |
| doi_str_mv | 10.1371/journal.pone.0116481 |
| format | Article |
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TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.
Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP).</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0116481</identifier><identifier>PMID: 25615613</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Allergies ; Alveoli ; Animals ; Bronchoalveolar lavage ; Bronchoalveolar Lavage Fluid - chemistry ; Bronchus ; Carbon ; Carbon nanotubes ; Crystal structure ; Crystallinity ; Electron microscopy ; Enclosures ; Endocytosis ; Exposure ; Female ; Gene expression ; Health sciences ; House mouse ; Immunology ; Inflammation ; Leukocytes (eosinophilic) ; Lung - drug effects ; Lung - ultrastructure ; Lungs ; Macrophages ; Mice ; Mice, Inbred C57BL ; Microscopy, Electron, Transmission - methods ; Nanotechnology ; Nanotubes ; Nanotubes, Carbon - toxicity ; Nanotubes, Carbon - ultrastructure ; Neutrophils ; Particle Size ; Pneumonia ; Pulmonary Eosinophilia - chemically induced ; Studies ; Time Factors ; Toxicology ; Transmission electron microscopy</subject><ispartof>PloS one, 2015-01, Vol.10 (1), p.e0116481-e0116481</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Købler et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Købler et al 2015 Købler et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-849bd0fad6c07bfb5f9879c498be776f788ab30bf907d4bf03b497fadec763d03</citedby><cites>FETCH-LOGICAL-c692t-849bd0fad6c07bfb5f9879c498be776f788ab30bf907d4bf03b497fadec763d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304811/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304811/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2104,2930,23873,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25615613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ceña, Valentin</contributor><creatorcontrib>Købler, Carsten</creatorcontrib><creatorcontrib>Poulsen, Sarah S</creatorcontrib><creatorcontrib>Saber, Anne T</creatorcontrib><creatorcontrib>Jacobsen, Nicklas R</creatorcontrib><creatorcontrib>Wallin, Håkan</creatorcontrib><creatorcontrib>Yauk, Carole L</creatorcontrib><creatorcontrib>Halappanavar, Sabina</creatorcontrib><creatorcontrib>Vogel, Ulla</creatorcontrib><creatorcontrib>Qvortrup, Klaus</creatorcontrib><creatorcontrib>Mølhave, Kristian</creatorcontrib><title>Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.
TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.
Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP).</description><subject>Allergies</subject><subject>Alveoli</subject><subject>Animals</subject><subject>Bronchoalveolar lavage</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Bronchus</subject><subject>Carbon</subject><subject>Carbon nanotubes</subject><subject>Crystal structure</subject><subject>Crystallinity</subject><subject>Electron microscopy</subject><subject>Enclosures</subject><subject>Endocytosis</subject><subject>Exposure</subject><subject>Female</subject><subject>Gene expression</subject><subject>Health sciences</subject><subject>House mouse</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lung - drug effects</subject><subject>Lung - ultrastructure</subject><subject>Lungs</subject><subject>Macrophages</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Electron, Transmission - methods</subject><subject>Nanotechnology</subject><subject>Nanotubes</subject><subject>Nanotubes, Carbon - toxicity</subject><subject>Nanotubes, Carbon - ultrastructure</subject><subject>Neutrophils</subject><subject>Particle Size</subject><subject>Pneumonia</subject><subject>Pulmonary Eosinophilia - chemically induced</subject><subject>Studies</subject><subject>Time Factors</subject><subject>Toxicology</subject><subject>Transmission electron microscopy</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7of-A9GCIHoxY9KkSXsjLIsfAwsLuop3IR8nMxk6yZi04v57MzPdZSp7IS20nDznzcl7coriBUZzTDh-vw5D9LKbb4OHOcKY0QY_Kk5xS6oZqxB5fPR_UpyltEaoJg1jT4uTqmY4v-S0-HnjNjAzsAVvwPdlGpSGrhs6GUvjUh-dGnoXfCm9KcFa0H0qgy21jCpHvfShHxSk0vmyG_xyv7hxGp4VT6zsEjwfv-fF908fby6_zK6uPy8uL65mmrVVP2toqwyy0jCNuLKqtm3DW03bRgHnzPKmkYogZVvEDVUWEUVbnnnQnBGDyHnx6qC77UISoylJYFZjTjAiVSYWB8IEuRbb6DYy3oogndgHQlwKGXunOxBYQdW0hnGQNUV1I3MBAKbK1RDWUpq1Poy7DWoDRmfLouwmotMV71ZiGX4LSlDuD84Cb0eBGH4NkHqxcWnnuPQQhn3dFcW8aklGX_-DPny6kVrKfADnbcj76p2ouKBVVVPOyc6l-QNUfgzkZuUbZF2OTxLeTRIy08OffimHlMTi29f_Z69_TNk3R-wKZNevUuj2dyxNQXoAdQwpRbD3JmMkdgNw54bYDYAYByCnvTxu0H3S3Y0nfwHoLwCz</recordid><startdate>20150123</startdate><enddate>20150123</enddate><creator>Købler, Carsten</creator><creator>Poulsen, Sarah S</creator><creator>Saber, Anne T</creator><creator>Jacobsen, Nicklas R</creator><creator>Wallin, Håkan</creator><creator>Yauk, Carole L</creator><creator>Halappanavar, Sabina</creator><creator>Vogel, Ulla</creator><creator>Qvortrup, Klaus</creator><creator>Mølhave, Kristian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150123</creationdate><title>Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice</title><author>Købler, Carsten ; Poulsen, Sarah S ; Saber, Anne T ; Jacobsen, Nicklas R ; Wallin, Håkan ; Yauk, Carole L ; Halappanavar, Sabina ; Vogel, Ulla ; Qvortrup, Klaus ; Mølhave, Kristian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-849bd0fad6c07bfb5f9879c498be776f788ab30bf907d4bf03b497fadec763d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Allergies</topic><topic>Alveoli</topic><topic>Animals</topic><topic>Bronchoalveolar lavage</topic><topic>Bronchoalveolar Lavage Fluid - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Købler, Carsten</au><au>Poulsen, Sarah S</au><au>Saber, Anne T</au><au>Jacobsen, Nicklas R</au><au>Wallin, Håkan</au><au>Yauk, Carole L</au><au>Halappanavar, Sabina</au><au>Vogel, Ulla</au><au>Qvortrup, Klaus</au><au>Mølhave, Kristian</au><au>Ceña, Valentin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-01-23</date><risdate>2015</risdate><volume>10</volume><issue>1</issue><spage>e0116481</spage><epage>e0116481</epage><pages>e0116481-e0116481</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.
TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.
Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP).</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25615613</pmid><doi>10.1371/journal.pone.0116481</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Allergies Alveoli Animals Bronchoalveolar lavage Bronchoalveolar Lavage Fluid - chemistry Bronchus Carbon Carbon nanotubes Crystal structure Crystallinity Electron microscopy Enclosures Endocytosis Exposure Female Gene expression Health sciences House mouse Immunology Inflammation Leukocytes (eosinophilic) Lung - drug effects Lung - ultrastructure Lungs Macrophages Mice Mice, Inbred C57BL Microscopy, Electron, Transmission - methods Nanotechnology Nanotubes Nanotubes, Carbon - toxicity Nanotubes, Carbon - ultrastructure Neutrophils Particle Size Pneumonia Pulmonary Eosinophilia - chemically induced Studies Time Factors Toxicology Transmission electron microscopy |
| title | Time-dependent subcellular distribution and effects of carbon nanotubes in lungs of mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T20%3A27%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Time-dependent%20subcellular%20distribution%20and%20effects%20of%20carbon%20nanotubes%20in%20lungs%20of%20mice&rft.jtitle=PloS%20one&rft.au=K%C3%B8bler,%20Carsten&rft.date=2015-01-23&rft.volume=10&rft.issue=1&rft.spage=e0116481&rft.epage=e0116481&rft.pages=e0116481-e0116481&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0116481&rft_dat=%3Cgale_plos_%3EA422547730%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1651731032&rft_id=info:pmid/25615613&rft_galeid=A422547730&rft_doaj_id=oai_doaj_org_article_1be289d67ea54058a6f7eed298b36944&rfr_iscdi=true |