Treatment of irradiated mice with high-dose ascorbic acid reduced lethality
Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by int...
Gespeichert in:
| Veröffentlicht in: | PloS one 2015-02, Vol.10 (2), p.e0117020-e0117020 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0117020 |
|---|---|
| container_issue | 2 |
| container_start_page | e0117020 |
| container_title | PloS one |
| container_volume | 10 |
| creator | Sato, Tomohito Kinoshita, Manabu Yamamoto, Tetsuo Ito, Masataka Nishida, Takafumi Takeuchi, Masaru Saitoh, Daizoh Seki, Shuhji Mukai, Yasuo |
| description | Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by intraperitoneal administration of ascorbic acid. Administration of 3 g/kg of ascorbic acid immediately after exposure significantly increased mouse survival after WBI at 7 to 8 Gy. However, administration of less than 3 g/kg of ascorbic acid was ineffective, and 4 or more g/kg was harmful to the mice. Post-exposure treatment with 3 g/kg of ascorbic acid reduced radiation-induced apoptosis in bone marrow cells and restored hematopoietic function. Treatment with ascorbic acid (3 g/kg) up to 24 h (1, 6, 12, or 24 h) after WBI at 7.5 Gy effectively improved mouse survival; however, treatments beyond 36 h were ineffective. Two treatments with ascorbic acid (1.5 g/kg × 2, immediately and 24 h after radiation, 3 g/kg in total) also improved mouse survival after WBI at 7.5 Gy, accompanied with suppression of radiation-induced free radical metabolites. In conclusion, administration of high-dose ascorbic acid might reduce radiation lethality in mice even after exposure. |
| doi_str_mv | 10.1371/journal.pone.0117020 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1651360832</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A425976467</galeid><doaj_id>oai_doaj_org_article_3845345113ea44f9b1c4e174f6f58515</doaj_id><sourcerecordid>A425976467</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-9e5478f48cecf74a06e9db6213d699df50817d866293141e287864fb41e772e13</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhgdRbK3-A9EBQfRi13xn5kYoxY_FQkGrtyGbnOykzE7WJKP235vtTsuu9EJykZA85835eKvqOUZzTCV-dxXGOOh-vgkDzBHGEhH0oDrGLSUzQRB9uHc-qp6kdIUQp40Qj6sjwgXHpG2Oqy-XEXRew5Dr4Gofo7ZeZ7D12huof_vc1Z1fdTMbEtQ6mRCX3tTaeFtHsKMpZA-5073P10-rR073CZ5N-0n1_eOHy7PPs_OLT4uz0_OZES3JsxY4k41jjQHjJNNIQGuXgmBqRdtax1GDpS2JkpZihoE0shHMLctRSgKYnlQvd7qbPiQ19SEpXGqiAjWUFGKxI2zQV2oT_VrHaxW0VzcXIa6UjtmbHhRtGKeMY0xBM-baJTYMsGROON5wzIvW--m3cbkGa0qrou4PRA9fBt-pVfilGMUSN7QIvJkEYvg5Qspq7ZOBvtcDhPEmb8I4R6It6Kt_0Purm6iVLgX4wYXyr9mKqlNGeCsFE7JQ83uosiyU2RbTOF_uDwLeHgQUJsOfvNJjSmrx7ev_sxc_DtnXe2wHus9dCv2YfRjSIch2oIkhpQjurskYqa3nb7uhtp5Xk-dL2Iv9Ad0F3Zqc_gVbnPmX</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1651360832</pqid></control><display><type>article</type><title>Treatment of irradiated mice with high-dose ascorbic acid reduced lethality</title><source>MEDLINE</source><source>Full-Text Journals in Chemistry (Open access)</source><source>PubMed Central_</source><source>Directory of Open Access Journals</source><source>PLoS (Open access)</source><source>EZB Electronic Journals Library</source><creator>Sato, Tomohito ; Kinoshita, Manabu ; Yamamoto, Tetsuo ; Ito, Masataka ; Nishida, Takafumi ; Takeuchi, Masaru ; Saitoh, Daizoh ; Seki, Shuhji ; Mukai, Yasuo</creator><contributor>Mezey, Eva</contributor><creatorcontrib>Sato, Tomohito ; Kinoshita, Manabu ; Yamamoto, Tetsuo ; Ito, Masataka ; Nishida, Takafumi ; Takeuchi, Masaru ; Saitoh, Daizoh ; Seki, Shuhji ; Mukai, Yasuo ; Mezey, Eva</creatorcontrib><description>Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by intraperitoneal administration of ascorbic acid. Administration of 3 g/kg of ascorbic acid immediately after exposure significantly increased mouse survival after WBI at 7 to 8 Gy. However, administration of less than 3 g/kg of ascorbic acid was ineffective, and 4 or more g/kg was harmful to the mice. Post-exposure treatment with 3 g/kg of ascorbic acid reduced radiation-induced apoptosis in bone marrow cells and restored hematopoietic function. Treatment with ascorbic acid (3 g/kg) up to 24 h (1, 6, 12, or 24 h) after WBI at 7.5 Gy effectively improved mouse survival; however, treatments beyond 36 h were ineffective. Two treatments with ascorbic acid (1.5 g/kg × 2, immediately and 24 h after radiation, 3 g/kg in total) also improved mouse survival after WBI at 7.5 Gy, accompanied with suppression of radiation-induced free radical metabolites. In conclusion, administration of high-dose ascorbic acid might reduce radiation lethality in mice even after exposure.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117020</identifier><identifier>PMID: 25651298</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Animals ; Antioxidants ; Antioxidants - administration & dosage ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - radiation effects ; Ascorbic acid ; Ascorbic Acid - administration & dosage ; Blood ; Bone marrow ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - radiation effects ; Casualties ; Cytokines ; Drug dosages ; Exposure ; Free radicals ; Hematology ; Hematopoiesis - drug effects ; Hematopoiesis - radiation effects ; Immunology ; Irradiation ; Lethality ; Male ; Medicine ; Metabolites ; Mice ; Mice, Inbred C57BL ; Military medicine ; Post-radiation ; Radiation ; Radiation effects ; Radiation, Ionizing ; Radiation-Protective Agents - administration & dosage ; Self defense ; Survival ; Survival Analysis ; Transplants & implants ; Vitamin C ; Whole-Body Irradiation</subject><ispartof>PloS one, 2015-02, Vol.10 (2), p.e0117020-e0117020</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Sato et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Sato et al 2015 Sato et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9e5478f48cecf74a06e9db6213d699df50817d866293141e287864fb41e772e13</citedby><cites>FETCH-LOGICAL-c692t-9e5478f48cecf74a06e9db6213d699df50817d866293141e287864fb41e772e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4317183/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4317183/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25651298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mezey, Eva</contributor><creatorcontrib>Sato, Tomohito</creatorcontrib><creatorcontrib>Kinoshita, Manabu</creatorcontrib><creatorcontrib>Yamamoto, Tetsuo</creatorcontrib><creatorcontrib>Ito, Masataka</creatorcontrib><creatorcontrib>Nishida, Takafumi</creatorcontrib><creatorcontrib>Takeuchi, Masaru</creatorcontrib><creatorcontrib>Saitoh, Daizoh</creatorcontrib><creatorcontrib>Seki, Shuhji</creatorcontrib><creatorcontrib>Mukai, Yasuo</creatorcontrib><title>Treatment of irradiated mice with high-dose ascorbic acid reduced lethality</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by intraperitoneal administration of ascorbic acid. Administration of 3 g/kg of ascorbic acid immediately after exposure significantly increased mouse survival after WBI at 7 to 8 Gy. However, administration of less than 3 g/kg of ascorbic acid was ineffective, and 4 or more g/kg was harmful to the mice. Post-exposure treatment with 3 g/kg of ascorbic acid reduced radiation-induced apoptosis in bone marrow cells and restored hematopoietic function. Treatment with ascorbic acid (3 g/kg) up to 24 h (1, 6, 12, or 24 h) after WBI at 7.5 Gy effectively improved mouse survival; however, treatments beyond 36 h were ineffective. Two treatments with ascorbic acid (1.5 g/kg × 2, immediately and 24 h after radiation, 3 g/kg in total) also improved mouse survival after WBI at 7.5 Gy, accompanied with suppression of radiation-induced free radical metabolites. In conclusion, administration of high-dose ascorbic acid might reduce radiation lethality in mice even after exposure.</description><subject>Acids</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - administration & dosage</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - radiation effects</subject><subject>Ascorbic acid</subject><subject>Ascorbic Acid - administration & dosage</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - radiation effects</subject><subject>Casualties</subject><subject>Cytokines</subject><subject>Drug dosages</subject><subject>Exposure</subject><subject>Free radicals</subject><subject>Hematology</subject><subject>Hematopoiesis - drug effects</subject><subject>Hematopoiesis - radiation effects</subject><subject>Immunology</subject><subject>Irradiation</subject><subject>Lethality</subject><subject>Male</subject><subject>Medicine</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Military medicine</subject><subject>Post-radiation</subject><subject>Radiation</subject><subject>Radiation effects</subject><subject>Radiation, Ionizing</subject><subject>Radiation-Protective Agents - administration & dosage</subject><subject>Self defense</subject><subject>Survival</subject><subject>Survival Analysis</subject><subject>Transplants & implants</subject><subject>Vitamin C</subject><subject>Whole-Body Irradiation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A9EBQfRi13xn5kYoxY_FQkGrtyGbnOykzE7WJKP235vtTsuu9EJykZA85835eKvqOUZzTCV-dxXGOOh-vgkDzBHGEhH0oDrGLSUzQRB9uHc-qp6kdIUQp40Qj6sjwgXHpG2Oqy-XEXRew5Dr4Gofo7ZeZ7D12huof_vc1Z1fdTMbEtQ6mRCX3tTaeFtHsKMpZA-5073P10-rR073CZ5N-0n1_eOHy7PPs_OLT4uz0_OZES3JsxY4k41jjQHjJNNIQGuXgmBqRdtax1GDpS2JkpZihoE0shHMLctRSgKYnlQvd7qbPiQ19SEpXGqiAjWUFGKxI2zQV2oT_VrHaxW0VzcXIa6UjtmbHhRtGKeMY0xBM-baJTYMsGROON5wzIvW--m3cbkGa0qrou4PRA9fBt-pVfilGMUSN7QIvJkEYvg5Qspq7ZOBvtcDhPEmb8I4R6It6Kt_0Purm6iVLgX4wYXyr9mKqlNGeCsFE7JQ83uosiyU2RbTOF_uDwLeHgQUJsOfvNJjSmrx7ev_sxc_DtnXe2wHus9dCv2YfRjSIch2oIkhpQjurskYqa3nb7uhtp5Xk-dL2Iv9Ad0F3Zqc_gVbnPmX</recordid><startdate>20150204</startdate><enddate>20150204</enddate><creator>Sato, Tomohito</creator><creator>Kinoshita, Manabu</creator><creator>Yamamoto, Tetsuo</creator><creator>Ito, Masataka</creator><creator>Nishida, Takafumi</creator><creator>Takeuchi, Masaru</creator><creator>Saitoh, Daizoh</creator><creator>Seki, Shuhji</creator><creator>Mukai, Yasuo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150204</creationdate><title>Treatment of irradiated mice with high-dose ascorbic acid reduced lethality</title><author>Sato, Tomohito ; Kinoshita, Manabu ; Yamamoto, Tetsuo ; Ito, Masataka ; Nishida, Takafumi ; Takeuchi, Masaru ; Saitoh, Daizoh ; Seki, Shuhji ; Mukai, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9e5478f48cecf74a06e9db6213d699df50817d866293141e287864fb41e772e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - administration & dosage</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - radiation effects</topic><topic>Ascorbic acid</topic><topic>Ascorbic Acid - administration & dosage</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - radiation effects</topic><topic>Casualties</topic><topic>Cytokines</topic><topic>Drug dosages</topic><topic>Exposure</topic><topic>Free radicals</topic><topic>Hematology</topic><topic>Hematopoiesis - drug effects</topic><topic>Hematopoiesis - radiation effects</topic><topic>Immunology</topic><topic>Irradiation</topic><topic>Lethality</topic><topic>Male</topic><topic>Medicine</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Military medicine</topic><topic>Post-radiation</topic><topic>Radiation</topic><topic>Radiation effects</topic><topic>Radiation, Ionizing</topic><topic>Radiation-Protective Agents - administration & dosage</topic><topic>Self defense</topic><topic>Survival</topic><topic>Survival Analysis</topic><topic>Transplants & implants</topic><topic>Vitamin C</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Tomohito</creatorcontrib><creatorcontrib>Kinoshita, Manabu</creatorcontrib><creatorcontrib>Yamamoto, Tetsuo</creatorcontrib><creatorcontrib>Ito, Masataka</creatorcontrib><creatorcontrib>Nishida, Takafumi</creatorcontrib><creatorcontrib>Takeuchi, Masaru</creatorcontrib><creatorcontrib>Saitoh, Daizoh</creatorcontrib><creatorcontrib>Seki, Shuhji</creatorcontrib><creatorcontrib>Mukai, Yasuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Tomohito</au><au>Kinoshita, Manabu</au><au>Yamamoto, Tetsuo</au><au>Ito, Masataka</au><au>Nishida, Takafumi</au><au>Takeuchi, Masaru</au><au>Saitoh, Daizoh</au><au>Seki, Shuhji</au><au>Mukai, Yasuo</au><au>Mezey, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treatment of irradiated mice with high-dose ascorbic acid reduced lethality</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-02-04</date><risdate>2015</risdate><volume>10</volume><issue>2</issue><spage>e0117020</spage><epage>e0117020</epage><pages>e0117020-e0117020</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by intraperitoneal administration of ascorbic acid. Administration of 3 g/kg of ascorbic acid immediately after exposure significantly increased mouse survival after WBI at 7 to 8 Gy. However, administration of less than 3 g/kg of ascorbic acid was ineffective, and 4 or more g/kg was harmful to the mice. Post-exposure treatment with 3 g/kg of ascorbic acid reduced radiation-induced apoptosis in bone marrow cells and restored hematopoietic function. Treatment with ascorbic acid (3 g/kg) up to 24 h (1, 6, 12, or 24 h) after WBI at 7.5 Gy effectively improved mouse survival; however, treatments beyond 36 h were ineffective. Two treatments with ascorbic acid (1.5 g/kg × 2, immediately and 24 h after radiation, 3 g/kg in total) also improved mouse survival after WBI at 7.5 Gy, accompanied with suppression of radiation-induced free radical metabolites. In conclusion, administration of high-dose ascorbic acid might reduce radiation lethality in mice even after exposure.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25651298</pmid><doi>10.1371/journal.pone.0117020</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-02, Vol.10 (2), p.e0117020-e0117020 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1651360832 |
| source | MEDLINE; Full-Text Journals in Chemistry (Open access); PubMed Central_; Directory of Open Access Journals; PLoS (Open access); EZB Electronic Journals Library |
| subjects | Acids Animals Antioxidants Antioxidants - administration & dosage Apoptosis Apoptosis - drug effects Apoptosis - radiation effects Ascorbic acid Ascorbic Acid - administration & dosage Blood Bone marrow Bone Marrow Cells - drug effects Bone Marrow Cells - radiation effects Casualties Cytokines Drug dosages Exposure Free radicals Hematology Hematopoiesis - drug effects Hematopoiesis - radiation effects Immunology Irradiation Lethality Male Medicine Metabolites Mice Mice, Inbred C57BL Military medicine Post-radiation Radiation Radiation effects Radiation, Ionizing Radiation-Protective Agents - administration & dosage Self defense Survival Survival Analysis Transplants & implants Vitamin C Whole-Body Irradiation |
| title | Treatment of irradiated mice with high-dose ascorbic acid reduced lethality |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-03T14%3A06%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Treatment%20of%20irradiated%20mice%20with%20high-dose%20ascorbic%20acid%20reduced%20lethality&rft.jtitle=PloS%20one&rft.au=Sato,%20Tomohito&rft.date=2015-02-04&rft.volume=10&rft.issue=2&rft.spage=e0117020&rft.epage=e0117020&rft.pages=e0117020-e0117020&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117020&rft_dat=%3Cgale_plos_%3EA425976467%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1651360832&rft_id=info:pmid/25651298&rft_galeid=A425976467&rft_doaj_id=oai_doaj_org_article_3845345113ea44f9b1c4e174f6f58515&rfr_iscdi=true |