The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia
Primary immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with an unclear etiology. This study aims to investigate the role of IL-23/Th17 pathway in patients with ITP. The gene expressions of IL-17, IL-23 and their receptors in ITP patients and healthy controls were analyzed by quanti...
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| Veröffentlicht in: | PloS one 2015-01, Vol.10 (1), p.e0117704-e0117704 |
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| creator | Ye, Xin Zhang, Lei Wang, Hui Chen, Yan Zhang, Weiwei Zhu, Rongrong Fang, Chaoping Deng, Anmei Qian, Baohua |
| description | Primary immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with an unclear etiology. This study aims to investigate the role of IL-23/Th17 pathway in patients with ITP.
The gene expressions of IL-17, IL-23 and their receptors in ITP patients and healthy controls were analyzed by quantitative real-time PCR. ELISA was used to test the IL-17 and IL-23 levels in plasma. Flow cytometry was used to detect the frequency of Th17 cells. The correlation between plasma IL-23 and IL-17 levels, Th17 cells, platelets were analyzed. The level of Th17-related cytokines was measured by ELISA following stimulation with IL-23. Subsequently, the IL-23 and IL-17 levels were measured in patients post-treatment.
The PBMCs of ITP patients showed increased mRNA expression levels in each of the following: IL-23p19, IL-12p40, IL-23R, IL-12Rβ1, IL-17A, IL-17F, and RORC. In addition, elevated Th17 cells and plasma IL-17, IL-23 levels were also observed in these ITP patients. Furthermore, it was found that IL-23 levels in plasma are positively correlated with IL-17 levels and Th17 cells, yet negatively correlated with platelet count. Following IL-23 stimulation in vitro, IL-17 levels showed significant elevation. Furthermore, both IL-23 and IL-17 levels decreased after effective treatment.
The IL-23/Th17 pathway may be involved in the pathogenesis of ITP through enhancement of the Th17 response. Moreover, our results suggest that the IL-23/Th17 pathway is a potential therapeutic target in future attempts of ITP treatment. |
| doi_str_mv | 10.1371/journal.pone.0117704 |
| format | Article |
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The gene expressions of IL-17, IL-23 and their receptors in ITP patients and healthy controls were analyzed by quantitative real-time PCR. ELISA was used to test the IL-17 and IL-23 levels in plasma. Flow cytometry was used to detect the frequency of Th17 cells. The correlation between plasma IL-23 and IL-17 levels, Th17 cells, platelets were analyzed. The level of Th17-related cytokines was measured by ELISA following stimulation with IL-23. Subsequently, the IL-23 and IL-17 levels were measured in patients post-treatment.
The PBMCs of ITP patients showed increased mRNA expression levels in each of the following: IL-23p19, IL-12p40, IL-23R, IL-12Rβ1, IL-17A, IL-17F, and RORC. In addition, elevated Th17 cells and plasma IL-17, IL-23 levels were also observed in these ITP patients. Furthermore, it was found that IL-23 levels in plasma are positively correlated with IL-17 levels and Th17 cells, yet negatively correlated with platelet count. Following IL-23 stimulation in vitro, IL-17 levels showed significant elevation. Furthermore, both IL-23 and IL-17 levels decreased after effective treatment.
The IL-23/Th17 pathway may be involved in the pathogenesis of ITP through enhancement of the Th17 response. Moreover, our results suggest that the IL-23/Th17 pathway is a potential therapeutic target in future attempts of ITP treatment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0117704</identifier><identifier>PMID: 25621490</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Analysis ; Antigens ; Arthritis ; Autoimmune diseases ; Bleeding ; Care and treatment ; Case-Control Studies ; Cells, Cultured ; Correlation ; Cytokines ; Cytometry ; Development and progression ; Dexamethasone - therapeutic use ; Drug dosages ; Enzyme-linked immunosorbent assay ; Etiology ; Female ; Flow cytometry ; Gene Expression ; Glucocorticoids - therapeutic use ; Helper cells ; Hospitals ; Humans ; Idiopathic thrombocytopenic purpura ; Interleukin 12 ; Interleukin 17 ; Interleukin 23 ; Interleukin-17 - blood ; Interleukin-17 - genetics ; Interleukin-23 - blood ; Interleukin-23 - genetics ; Laboratories ; Lymphocytes ; Lymphocytes T ; Male ; Middle Aged ; Pathogenesis ; Patients ; Pediatrics ; Platelet Count ; Platelets ; Purpura, Thrombocytopenic, Idiopathic - blood ; Purpura, Thrombocytopenic, Idiopathic - drug therapy ; Purpura, Thrombocytopenic, Idiopathic - immunology ; Receptors ; RNA ; Stimulation ; Studies ; Systemic lupus erythematosus ; Th17 Cells - physiology ; Thrombocytopenia ; Young Adult</subject><ispartof>PloS one, 2015-01, Vol.10 (1), p.e0117704-e0117704</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Ye et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Ye et al 2015 Ye et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9fa4cf694aff91b09d5d3fae0433e2c37670ce14775030bc003ce30393c369593</citedby><cites>FETCH-LOGICAL-c692t-9fa4cf694aff91b09d5d3fae0433e2c37670ce14775030bc003ce30393c369593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306550/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306550/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25621490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kuwana, Masataka</contributor><creatorcontrib>Ye, Xin</creatorcontrib><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Chen, Yan</creatorcontrib><creatorcontrib>Zhang, Weiwei</creatorcontrib><creatorcontrib>Zhu, Rongrong</creatorcontrib><creatorcontrib>Fang, Chaoping</creatorcontrib><creatorcontrib>Deng, Anmei</creatorcontrib><creatorcontrib>Qian, Baohua</creatorcontrib><title>The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Primary immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with an unclear etiology. This study aims to investigate the role of IL-23/Th17 pathway in patients with ITP.
The gene expressions of IL-17, IL-23 and their receptors in ITP patients and healthy controls were analyzed by quantitative real-time PCR. ELISA was used to test the IL-17 and IL-23 levels in plasma. Flow cytometry was used to detect the frequency of Th17 cells. The correlation between plasma IL-23 and IL-17 levels, Th17 cells, platelets were analyzed. The level of Th17-related cytokines was measured by ELISA following stimulation with IL-23. Subsequently, the IL-23 and IL-17 levels were measured in patients post-treatment.
The PBMCs of ITP patients showed increased mRNA expression levels in each of the following: IL-23p19, IL-12p40, IL-23R, IL-12Rβ1, IL-17A, IL-17F, and RORC. In addition, elevated Th17 cells and plasma IL-17, IL-23 levels were also observed in these ITP patients. Furthermore, it was found that IL-23 levels in plasma are positively correlated with IL-17 levels and Th17 cells, yet negatively correlated with platelet count. Following IL-23 stimulation in vitro, IL-17 levels showed significant elevation. Furthermore, both IL-23 and IL-17 levels decreased after effective treatment.
The IL-23/Th17 pathway may be involved in the pathogenesis of ITP through enhancement of the Th17 response. Moreover, our results suggest that the IL-23/Th17 pathway is a potential therapeutic target in future attempts of ITP treatment.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis</subject><subject>Antigens</subject><subject>Arthritis</subject><subject>Autoimmune diseases</subject><subject>Bleeding</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Cells, Cultured</subject><subject>Correlation</subject><subject>Cytokines</subject><subject>Cytometry</subject><subject>Development and progression</subject><subject>Dexamethasone - therapeutic use</subject><subject>Drug dosages</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Etiology</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gene Expression</subject><subject>Glucocorticoids - therapeutic use</subject><subject>Helper cells</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Idiopathic thrombocytopenic purpura</subject><subject>Interleukin 12</subject><subject>Interleukin 17</subject><subject>Interleukin 23</subject><subject>Interleukin-17 - blood</subject><subject>Interleukin-17 - genetics</subject><subject>Interleukin-23 - blood</subject><subject>Interleukin-23 - genetics</subject><subject>Laboratories</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Platelet Count</subject><subject>Platelets</subject><subject>Purpura, Thrombocytopenic, Idiopathic - blood</subject><subject>Purpura, Thrombocytopenic, Idiopathic - drug therapy</subject><subject>Purpura, Thrombocytopenic, Idiopathic - immunology</subject><subject>Receptors</subject><subject>RNA</subject><subject>Stimulation</subject><subject>Studies</subject><subject>Systemic lupus erythematosus</subject><subject>Th17 Cells - physiology</subject><subject>Thrombocytopenia</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7jr6D0QLgujFzCbNR5sbYVn8GBgY0NHbcJom0yxtMzap6_57U6e7TGUvJBcNyXPek_P2TZKXGK0wyfHFtRv6DprVwXV6hTDOc0QfJedYkGzJM0Qen-zPkmfeXyPESMH50-QsYzzDVKDzZLurddq7RqfOpOvNMiMXuxrn6QFCfQO3qe3GrdVd8OmNDXV66G0Lfbxo26HTaah715ZO3QZ30J2F58kTA43XL6bvIvn-6ePu6stys_28vrrcLBUXWVgKA1QZLigYI3CJRMUqYkAjSojOFMl5jpTGNM8ZIqhUCBGlCSKCKMIFE2SRvD7qHhrn5eSFl5jTgsQ5WR6J9ZGoHFzL6dnSgZV_D1y_l9AHqxotS1BACYWKGUpNmQEgzCBXglekKGLvRfJh6jaUra5UtKOHZiY6v-lsLfful6QEccZGgXeTQO9-DtoH2VqvdNNAp90wvptlFGNRsIi--Qd9eLqJ2kMcwHbGxb5qFJWXNMPFuEaXVg9QcVW6tSoGx9h4Pit4PyuITNC_wx4G7-X629f_Z7c_5uzbE7bW0ITau2YI1nV-DtIjqHrnfa_NvckYyTH3d27IMfdyyn0se3X6g-6L7oJO_gAKpft1</recordid><startdate>20150126</startdate><enddate>20150126</enddate><creator>Ye, Xin</creator><creator>Zhang, Lei</creator><creator>Wang, Hui</creator><creator>Chen, Yan</creator><creator>Zhang, Weiwei</creator><creator>Zhu, Rongrong</creator><creator>Fang, Chaoping</creator><creator>Deng, Anmei</creator><creator>Qian, Baohua</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150126</creationdate><title>The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia</title><author>Ye, Xin ; Zhang, Lei ; Wang, Hui ; Chen, Yan ; Zhang, Weiwei ; Zhu, Rongrong ; Fang, Chaoping ; Deng, Anmei ; Qian, Baohua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9fa4cf694aff91b09d5d3fae0433e2c37670ce14775030bc003ce30393c369593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis</topic><topic>Antigens</topic><topic>Arthritis</topic><topic>Autoimmune diseases</topic><topic>Bleeding</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>Cells, Cultured</topic><topic>Correlation</topic><topic>Cytokines</topic><topic>Cytometry</topic><topic>Development and progression</topic><topic>Dexamethasone - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Xin</au><au>Zhang, Lei</au><au>Wang, Hui</au><au>Chen, Yan</au><au>Zhang, Weiwei</au><au>Zhu, Rongrong</au><au>Fang, Chaoping</au><au>Deng, Anmei</au><au>Qian, Baohua</au><au>Kuwana, Masataka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-01-26</date><risdate>2015</risdate><volume>10</volume><issue>1</issue><spage>e0117704</spage><epage>e0117704</epage><pages>e0117704-e0117704</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Primary immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with an unclear etiology. This study aims to investigate the role of IL-23/Th17 pathway in patients with ITP.
The gene expressions of IL-17, IL-23 and their receptors in ITP patients and healthy controls were analyzed by quantitative real-time PCR. ELISA was used to test the IL-17 and IL-23 levels in plasma. Flow cytometry was used to detect the frequency of Th17 cells. The correlation between plasma IL-23 and IL-17 levels, Th17 cells, platelets were analyzed. The level of Th17-related cytokines was measured by ELISA following stimulation with IL-23. Subsequently, the IL-23 and IL-17 levels were measured in patients post-treatment.
The PBMCs of ITP patients showed increased mRNA expression levels in each of the following: IL-23p19, IL-12p40, IL-23R, IL-12Rβ1, IL-17A, IL-17F, and RORC. In addition, elevated Th17 cells and plasma IL-17, IL-23 levels were also observed in these ITP patients. Furthermore, it was found that IL-23 levels in plasma are positively correlated with IL-17 levels and Th17 cells, yet negatively correlated with platelet count. Following IL-23 stimulation in vitro, IL-17 levels showed significant elevation. Furthermore, both IL-23 and IL-17 levels decreased after effective treatment.
The IL-23/Th17 pathway may be involved in the pathogenesis of ITP through enhancement of the Th17 response. Moreover, our results suggest that the IL-23/Th17 pathway is a potential therapeutic target in future attempts of ITP treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25621490</pmid><doi>10.1371/journal.pone.0117704</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1648300557 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Aged Analysis Antigens Arthritis Autoimmune diseases Bleeding Care and treatment Case-Control Studies Cells, Cultured Correlation Cytokines Cytometry Development and progression Dexamethasone - therapeutic use Drug dosages Enzyme-linked immunosorbent assay Etiology Female Flow cytometry Gene Expression Glucocorticoids - therapeutic use Helper cells Hospitals Humans Idiopathic thrombocytopenic purpura Interleukin 12 Interleukin 17 Interleukin 23 Interleukin-17 - blood Interleukin-17 - genetics Interleukin-23 - blood Interleukin-23 - genetics Laboratories Lymphocytes Lymphocytes T Male Middle Aged Pathogenesis Patients Pediatrics Platelet Count Platelets Purpura, Thrombocytopenic, Idiopathic - blood Purpura, Thrombocytopenic, Idiopathic - drug therapy Purpura, Thrombocytopenic, Idiopathic - immunology Receptors RNA Stimulation Studies Systemic lupus erythematosus Th17 Cells - physiology Thrombocytopenia Young Adult |
| title | The role of IL-23/Th17 pathway in patients with primary immune thrombocytopenia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T03%3A36%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20role%20of%20IL-23/Th17%20pathway%20in%20patients%20with%20primary%20immune%20thrombocytopenia&rft.jtitle=PloS%20one&rft.au=Ye,%20Xin&rft.date=2015-01-26&rft.volume=10&rft.issue=1&rft.spage=e0117704&rft.epage=e0117704&rft.pages=e0117704-e0117704&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0117704&rft_dat=%3Cgale_plos_%3EA421818189%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1648300557&rft_id=info:pmid/25621490&rft_galeid=A421818189&rft_doaj_id=oai_doaj_org_article_baca434ad5f44fb2aa015a7c96d38800&rfr_iscdi=true |