Molecular epidemiology and phylogenetic analyses of influenza B virus in Thailand during 2010 to 2014
Influenza B virus remains a major contributor to the seasonal influenza outbreak and its prevalence has increased worldwide. We investigated the epidemiology and analyzed the full genome sequences of influenza B virus strains in Thailand between 2010 and 2014. Samples from the upper respiratory trac...
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| Veröffentlicht in: | PloS one 2015-01, Vol.10 (1), p.e0116302 |
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| creator | Tewawong, Nipaporn Suwannakarn, Kamol Prachayangprecha, Slinporn Korkong, Sumeth Vichiwattana, Preeyaporn Vongpunsawad, Sompong Poovorawan, Yong |
| description | Influenza B virus remains a major contributor to the seasonal influenza outbreak and its prevalence has increased worldwide. We investigated the epidemiology and analyzed the full genome sequences of influenza B virus strains in Thailand between 2010 and 2014. Samples from the upper respiratory tract were collected from patients diagnosed with influenza like-illness. All samples were screened for influenza A/B viruses by one-step multiplex real-time RT-PCR. The whole genome of 53 influenza B isolates were amplified, sequenced, and analyzed. From 14,418 respiratory samples collected during 2010 to 2014, a total of 3,050 tested positive for influenza virus. Approximately 3.27% (471/14,418) were influenza B virus samples. Fifty three isolates of influenza B virus were randomly chosen for detailed whole genome analysis. Phylogenetic analysis of the HA gene showed clusters in Victoria clades 1A, 1B, 3, 5 and Yamagata clades 2 and 3. Both B/Victoria and B/Yamagata lineages were found to co-circulate during this time. The NA sequences of all isolates belonged to lineage II and consisted of viruses from both HA Victoria and Yamagata lineages, reflecting possible reassortment of the HA and NA genes. No significant changes were seen in the NA protein. The phylogenetic trees generated through the analysis of the PB1 and PB2 genes closely resembled that of the HA gene, while trees generated from the analysis of the PA, NP, and M genes showed similar topology. The NS gene exhibited the pattern of genetic reassortment distinct from those of the PA, NP or M genes. Thus, antigenic drift and genetic reassortment among the influenza B virus strains were observed in the isolates examined. Our findings indicate that the co-circulation of two distinct lineages of influenza B viruses and the limitation of cross-protection of the current vaccine formulation provide support for quadrivalent influenza vaccine in this region. |
| doi_str_mv | 10.1371/journal.pone.0116302 |
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We investigated the epidemiology and analyzed the full genome sequences of influenza B virus strains in Thailand between 2010 and 2014. Samples from the upper respiratory tract were collected from patients diagnosed with influenza like-illness. All samples were screened for influenza A/B viruses by one-step multiplex real-time RT-PCR. The whole genome of 53 influenza B isolates were amplified, sequenced, and analyzed. From 14,418 respiratory samples collected during 2010 to 2014, a total of 3,050 tested positive for influenza virus. Approximately 3.27% (471/14,418) were influenza B virus samples. Fifty three isolates of influenza B virus were randomly chosen for detailed whole genome analysis. Phylogenetic analysis of the HA gene showed clusters in Victoria clades 1A, 1B, 3, 5 and Yamagata clades 2 and 3. Both B/Victoria and B/Yamagata lineages were found to co-circulate during this time. The NA sequences of all isolates belonged to lineage II and consisted of viruses from both HA Victoria and Yamagata lineages, reflecting possible reassortment of the HA and NA genes. No significant changes were seen in the NA protein. The phylogenetic trees generated through the analysis of the PB1 and PB2 genes closely resembled that of the HA gene, while trees generated from the analysis of the PA, NP, and M genes showed similar topology. The NS gene exhibited the pattern of genetic reassortment distinct from those of the PA, NP or M genes. Thus, antigenic drift and genetic reassortment among the influenza B virus strains were observed in the isolates examined. Our findings indicate that the co-circulation of two distinct lineages of influenza B viruses and the limitation of cross-protection of the current vaccine formulation provide support for quadrivalent influenza vaccine in this region.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0116302</identifier><identifier>PMID: 25602617</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Analysis ; Antigenic drift ; Antigens ; Child ; Child, Preschool ; Cladistic analysis ; Cluster analysis ; Cross-protection ; Epidemiology ; Female ; Gene sequencing ; Genes ; Genomes ; Genomics ; Health aspects ; Humans ; Infections ; Influenza ; Influenza A ; Influenza A virus - classification ; Influenza A virus - genetics ; Influenza B ; Influenza B virus - classification ; Influenza B virus - genetics ; Influenza, Human - immunology ; Influenza, Human - virology ; Male ; Medicine ; Middle Aged ; Molecular Epidemiology ; Molecular Sequence Data ; Multiplexing ; Outbreaks ; Pandemics ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Respiratory tract ; Strains (organisms) ; Thailand - epidemiology ; Topology ; Trees ; Vaccines ; Virology ; Viruses ; Young Adult</subject><ispartof>PloS one, 2015-01, Vol.10 (1), p.e0116302</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Tewawong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Tewawong et al 2015 Tewawong et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-723724b2ad17ff110eff8b700cbf866039f6363fd585095673aae4123dd1a8963</citedby><cites>FETCH-LOGICAL-c692t-723724b2ad17ff110eff8b700cbf866039f6363fd585095673aae4123dd1a8963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300180/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4300180/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25602617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tewawong, Nipaporn</creatorcontrib><creatorcontrib>Suwannakarn, Kamol</creatorcontrib><creatorcontrib>Prachayangprecha, Slinporn</creatorcontrib><creatorcontrib>Korkong, Sumeth</creatorcontrib><creatorcontrib>Vichiwattana, Preeyaporn</creatorcontrib><creatorcontrib>Vongpunsawad, Sompong</creatorcontrib><creatorcontrib>Poovorawan, Yong</creatorcontrib><title>Molecular epidemiology and phylogenetic analyses of influenza B virus in Thailand during 2010 to 2014</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Influenza B virus remains a major contributor to the seasonal influenza outbreak and its prevalence has increased worldwide. We investigated the epidemiology and analyzed the full genome sequences of influenza B virus strains in Thailand between 2010 and 2014. Samples from the upper respiratory tract were collected from patients diagnosed with influenza like-illness. All samples were screened for influenza A/B viruses by one-step multiplex real-time RT-PCR. The whole genome of 53 influenza B isolates were amplified, sequenced, and analyzed. From 14,418 respiratory samples collected during 2010 to 2014, a total of 3,050 tested positive for influenza virus. Approximately 3.27% (471/14,418) were influenza B virus samples. Fifty three isolates of influenza B virus were randomly chosen for detailed whole genome analysis. Phylogenetic analysis of the HA gene showed clusters in Victoria clades 1A, 1B, 3, 5 and Yamagata clades 2 and 3. Both B/Victoria and B/Yamagata lineages were found to co-circulate during this time. The NA sequences of all isolates belonged to lineage II and consisted of viruses from both HA Victoria and Yamagata lineages, reflecting possible reassortment of the HA and NA genes. No significant changes were seen in the NA protein. The phylogenetic trees generated through the analysis of the PB1 and PB2 genes closely resembled that of the HA gene, while trees generated from the analysis of the PA, NP, and M genes showed similar topology. The NS gene exhibited the pattern of genetic reassortment distinct from those of the PA, NP or M genes. Thus, antigenic drift and genetic reassortment among the influenza B virus strains were observed in the isolates examined. Our findings indicate that the co-circulation of two distinct lineages of influenza B viruses and the limitation of cross-protection of the current vaccine formulation provide support for quadrivalent influenza vaccine in this region.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis</subject><subject>Antigenic drift</subject><subject>Antigens</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cladistic analysis</subject><subject>Cluster analysis</subject><subject>Cross-protection</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A virus - classification</subject><subject>Influenza A virus - genetics</subject><subject>Influenza B</subject><subject>Influenza B virus - classification</subject><subject>Influenza B virus - genetics</subject><subject>Influenza, Human - immunology</subject><subject>Influenza, Human - virology</subject><subject>Male</subject><subject>Medicine</subject><subject>Middle Aged</subject><subject>Molecular Epidemiology</subject><subject>Molecular Sequence Data</subject><subject>Multiplexing</subject><subject>Outbreaks</subject><subject>Pandemics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Respiratory tract</subject><subject>Strains (organisms)</subject><subject>Thailand - epidemiology</subject><subject>Topology</subject><subject>Trees</subject><subject>Vaccines</subject><subject>Virology</subject><subject>Viruses</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBEQkL0sIu_4iQXpFJBWamoEhSu1sSxs15549ROKpZfj8Om1Qb1gHywNX7mHc-MJ0leYrTENMfvN27wLdhl51q1RBhzisij5BiXlCw4QfTxwfkoeRbCBqGMFpw_TY5IxhHhOD9O1FdnlRws-FR1plZb46xrdim0ddqtd_GsWtUbGQ1gd0GF1OnUtNoOqv0N6cf01vghREt6vQZjR7d68KZtUoIwSns37ux58kSDDerFtJ8kPz5_uj7_sri8ulidn10uJC9Jv8gJzQmrCNQ41xpjpLQuqhwhWen4cERLzSmnus6KDJUZzymAYpjQusZQlJyeJK_3up11QUwVCgJzljOKMzwSqz1RO9iIzpst-J1wYMRfg_ONAB_ztUoALzKoJCq5xCyXuqCyYjgDXDIZ42dR68MUbai2qpaq7T3Ymej8pjVr0bhbwShCuEBR4N0k4N3NoEIvtiZIZWMZlRvGd2eEobKkLKJv_kEfzm6iGogJxDa5GFeOouKMEVrEuhVlpJYPUHGN7ZfxO2kT7TOH05lDZHr1q29gCEGsvn_7f_bq55x9e8CuFdh-HZwdeuPaMAfZHpTeheCVvi8yRmKchrtqiHEaxDQN0e3VYYPune6-P_0D7q0Cdg</recordid><startdate>20150120</startdate><enddate>20150120</enddate><creator>Tewawong, Nipaporn</creator><creator>Suwannakarn, Kamol</creator><creator>Prachayangprecha, Slinporn</creator><creator>Korkong, Sumeth</creator><creator>Vichiwattana, Preeyaporn</creator><creator>Vongpunsawad, Sompong</creator><creator>Poovorawan, Yong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150120</creationdate><title>Molecular epidemiology and phylogenetic analyses of influenza B virus in Thailand during 2010 to 2014</title><author>Tewawong, Nipaporn ; Suwannakarn, Kamol ; Prachayangprecha, Slinporn ; Korkong, Sumeth ; Vichiwattana, Preeyaporn ; Vongpunsawad, Sompong ; Poovorawan, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-723724b2ad17ff110eff8b700cbf866039f6363fd585095673aae4123dd1a8963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis</topic><topic>Antigenic drift</topic><topic>Antigens</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cladistic analysis</topic><topic>Cluster analysis</topic><topic>Cross-protection</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Infections</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Influenza A virus - 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We investigated the epidemiology and analyzed the full genome sequences of influenza B virus strains in Thailand between 2010 and 2014. Samples from the upper respiratory tract were collected from patients diagnosed with influenza like-illness. All samples were screened for influenza A/B viruses by one-step multiplex real-time RT-PCR. The whole genome of 53 influenza B isolates were amplified, sequenced, and analyzed. From 14,418 respiratory samples collected during 2010 to 2014, a total of 3,050 tested positive for influenza virus. Approximately 3.27% (471/14,418) were influenza B virus samples. Fifty three isolates of influenza B virus were randomly chosen for detailed whole genome analysis. Phylogenetic analysis of the HA gene showed clusters in Victoria clades 1A, 1B, 3, 5 and Yamagata clades 2 and 3. Both B/Victoria and B/Yamagata lineages were found to co-circulate during this time. The NA sequences of all isolates belonged to lineage II and consisted of viruses from both HA Victoria and Yamagata lineages, reflecting possible reassortment of the HA and NA genes. No significant changes were seen in the NA protein. The phylogenetic trees generated through the analysis of the PB1 and PB2 genes closely resembled that of the HA gene, while trees generated from the analysis of the PA, NP, and M genes showed similar topology. The NS gene exhibited the pattern of genetic reassortment distinct from those of the PA, NP or M genes. Thus, antigenic drift and genetic reassortment among the influenza B virus strains were observed in the isolates examined. Our findings indicate that the co-circulation of two distinct lineages of influenza B viruses and the limitation of cross-protection of the current vaccine formulation provide support for quadrivalent influenza vaccine in this region.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25602617</pmid><doi>10.1371/journal.pone.0116302</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Analysis Antigenic drift Antigens Child Child, Preschool Cladistic analysis Cluster analysis Cross-protection Epidemiology Female Gene sequencing Genes Genomes Genomics Health aspects Humans Infections Influenza Influenza A Influenza A virus - classification Influenza A virus - genetics Influenza B Influenza B virus - classification Influenza B virus - genetics Influenza, Human - immunology Influenza, Human - virology Male Medicine Middle Aged Molecular Epidemiology Molecular Sequence Data Multiplexing Outbreaks Pandemics Phylogenetics Phylogeny Polymerase chain reaction Respiratory tract Strains (organisms) Thailand - epidemiology Topology Trees Vaccines Virology Viruses Young Adult |
| title | Molecular epidemiology and phylogenetic analyses of influenza B virus in Thailand during 2010 to 2014 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T23%3A29%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20epidemiology%20and%20phylogenetic%20analyses%20of%20influenza%20B%20virus%20in%20Thailand%20during%202010%20to%202014&rft.jtitle=PloS%20one&rft.au=Tewawong,%20Nipaporn&rft.date=2015-01-20&rft.volume=10&rft.issue=1&rft.spage=e0116302&rft.pages=e0116302-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0116302&rft_dat=%3Cgale_plos_%3EA423858589%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1647431516&rft_id=info:pmid/25602617&rft_galeid=A423858589&rft_doaj_id=oai_doaj_org_article_a685abc096c147cf83cb415a194c8505&rfr_iscdi=true |