Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles
Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogen...
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| Veröffentlicht in: | PloS one 2015-01, Vol.10 (1), p.e0115857-e0115857 |
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| creator | Bernitt, Erik Koh, Cheng Gee Gov, Nir Döbereiner, Hans-Günther |
| description | Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. We show that theoretical models of reaction-diffusion type incorporating conserved species capture partially the behavior we observe in our data. |
| doi_str_mv | 10.1371/journal.pone.0115857 |
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In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. We show that theoretical models of reaction-diffusion type incorporating conserved species capture partially the behavior we observe in our data.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0115857</identifier><identifier>PMID: 25574668</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin ; Actins - metabolism ; Active control ; Analysis ; Animals ; Cell Membrane - drug effects ; Cell Membrane - physiology ; Cell Membrane - ultrastructure ; Cell Surface Extensions - drug effects ; Cell Surface Extensions - physiology ; Cell Surface Extensions - ultrastructure ; Fibroblasts ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - physiology ; Image Processing, Computer-Assisted ; Mice ; Microscopy, Fluorescence ; Models, Biological ; Muscle proteins ; NIH 3T3 Cells ; Platelet-Derived Growth Factor - pharmacology ; Quantitative analysis ; Species diffusion ; Substrates ; Time-Lapse Imaging ; Wave propagation</subject><ispartof>PloS one, 2015-01, Vol.10 (1), p.e0115857-e0115857</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Bernitt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Bernitt et al 2015 Bernitt et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-922b5f6d47211d71b2cd370b90ebecd4564bdad087526fe653c84fed68a5e0843</citedby><cites>FETCH-LOGICAL-c692t-922b5f6d47211d71b2cd370b90ebecd4564bdad087526fe653c84fed68a5e0843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289068/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289068/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25574668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nabi, Ivan R</contributor><creatorcontrib>Bernitt, Erik</creatorcontrib><creatorcontrib>Koh, Cheng Gee</creatorcontrib><creatorcontrib>Gov, Nir</creatorcontrib><creatorcontrib>Döbereiner, Hans-Günther</creatorcontrib><title>Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bernitt, Erik</au><au>Koh, Cheng Gee</au><au>Gov, Nir</au><au>Döbereiner, Hans-Günther</au><au>Nabi, Ivan R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-01-09</date><risdate>2015</risdate><volume>10</volume><issue>1</issue><spage>e0115857</spage><epage>e0115857</epage><pages>e0115857-e0115857</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. We show that theoretical models of reaction-diffusion type incorporating conserved species capture partially the behavior we observe in our data.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25574668</pmid><doi>10.1371/journal.pone.0115857</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Actins - metabolism Active control Analysis Animals Cell Membrane - drug effects Cell Membrane - physiology Cell Membrane - ultrastructure Cell Surface Extensions - drug effects Cell Surface Extensions - physiology Cell Surface Extensions - ultrastructure Fibroblasts Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - physiology Image Processing, Computer-Assisted Mice Microscopy, Fluorescence Models, Biological Muscle proteins NIH 3T3 Cells Platelet-Derived Growth Factor - pharmacology Quantitative analysis Species diffusion Substrates Time-Lapse Imaging Wave propagation |
| title | Dynamics of actin waves on patterned substrates: a quantitative analysis of circular dorsal ruffles |
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