Derivation of iPSCs after culture of human dental pulp cells under defined conditions

Human dental pulp cells (hDPCs) are a promising resource for regenerative medicine and tissue engineering and can be used for derivation of induced pluripotent stem cells (iPSCs). However, current protocols use reagents of animal origin (mainly fetal bovine serum, FBS) that carry the potential risk...

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Veröffentlicht in:	PloS one 2014-12, Vol.9 (12), p.e115392-e115392
Hauptverfasser:	Takeda-Kawaguchi, Tomoko, Sugiyama, Ken, Chikusa, Shunji, Iida, Kazuki, Aoki, Hitomi, Tamaoki, Naritaka, Hatakeyama, Daijiro, Kunisada, Takahiro, Shibata, Toshiyuki, Fusaki, Noemi, Tezuka, Ken-Ichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Analysis Biology and Life Sciences Cell culture Cell Differentiation Cells, Cultured Dental pulp Dental Pulp - cytology Deoxyribonucleic acid Derivation Differentiation DNA DNA microarrays Experiments Fetal calf serum Gene expression Genomes Haplotypes Health risks Humans Immunogenicity Induced Pluripotent Stem Cells - cytology Infectious diseases Medicine Pluripotency Primary Cell Culture - methods Reagents Regenerative medicine Science Stem cell transplantation Stem cells Tissue engineering Transplants & implants University graduates Viruses
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