The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture
To reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium Pseudomonas stutzeri, SC221-M, was obtained. The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression...
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| creator | Deng, Bin Fu, Luoqin Zhang, Xiaoping Zheng, Jiajia Peng, Lisha Sun, Jiandong Zhu, Haiyan Wang, Yibing Li, Weifen Wu, Xuexiang Wu, Di |
| description | To reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium Pseudomonas stutzeri, SC221-M, was obtained. The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35°C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3× mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24) was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems. |
| doi_str_mv | 10.1371/journal.pone.0114886 |
| format | Article |
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The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35°C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3× mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24) was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0114886</identifier><identifier>PMID: 25489740</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ammonium ; Animal sciences ; Animals ; Aquaculture ; Aquaculture industry ; Bacillus ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biology and Life Sciences ; Carbon ; Carbon sources ; Carp ; Carps ; Chemical oxygen demand ; Citric acid ; Communities ; Community structure ; Ctenopharyngodon idella ; Denitrification ; Denitrification - physiology ; Earth Sciences ; Ecology and Environmental Sciences ; Environmental regulations ; Feed science ; Feeds ; Fish ; Gene Expression ; Genomes ; Laboratories ; Microorganisms ; Nitrites - metabolism ; Nitrogen ; Nitrogen removal ; Nitrous oxide ; Nutrition ; Oxygen ; Pollutants ; Pseudomonas ; Pseudomonas mandelii ; Pseudomonas stutzeri ; Pseudomonas stutzeri - isolation & purification ; Pseudomonas stutzeri - physiology ; Quality Control ; Sodium ; Sodium citrate ; Sodium nitrite ; Temperature ; Temperature range ; Total dissolved solids ; Water Purification - methods ; Water Quality ; Water quality control ; Zoology</subject><ispartof>PloS one, 2014-12, Vol.9 (12), p.e114886-e114886</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Deng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35°C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3× mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24) was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems.</description><subject>Ammonium</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Aquaculture industry</subject><subject>Bacillus</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Carp</subject><subject>Carps</subject><subject>Chemical oxygen demand</subject><subject>Citric acid</subject><subject>Communities</subject><subject>Community structure</subject><subject>Ctenopharyngodon idella</subject><subject>Denitrification</subject><subject>Denitrification - physiology</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Environmental regulations</subject><subject>Feed science</subject><subject>Feeds</subject><subject>Fish</subject><subject>Gene Expression</subject><subject>Genomes</subject><subject>Laboratories</subject><subject>Microorganisms</subject><subject>Nitrites - metabolism</subject><subject>Nitrogen</subject><subject>Nitrogen removal</subject><subject>Nitrous oxide</subject><subject>Nutrition</subject><subject>Oxygen</subject><subject>Pollutants</subject><subject>Pseudomonas</subject><subject>Pseudomonas mandelii</subject><subject>Pseudomonas stutzeri</subject><subject>Pseudomonas stutzeri - isolation & purification</subject><subject>Pseudomonas stutzeri - physiology</subject><subject>Quality Control</subject><subject>Sodium</subject><subject>Sodium citrate</subject><subject>Sodium nitrite</subject><subject>Temperature</subject><subject>Temperature range</subject><subject>Total dissolved solids</subject><subject>Water Purification - methods</subject><subject>Water Quality</subject><subject>Water quality control</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggsISG42MWnOPFNpWrFoVJRES3cWrPOZNeVN97aDlAegacmaXerLuoFyoUjzzf_2P94iuI5o1MmKvbuIvSxAz9dhw6nlDFZ1-pBsc-04BPFqXh453-veJLSBaWlqJV6XOzxUta6knS_-HO-RNJg53J0rbOQXeiIXUIEmzG6lJ1NJLTkS8K-CavQQSIp9_n3ECRnM87Z5DOBriEuJwLrtd9q5EB-wiBBLnvwLl8RG7ocgyeuI4sIKRELcU1gCNve5z7i0-JRCz7hs816UHz78P589mlycvrxeHZ0MrFK8zzRLedzO29kXVqtQdVYKirmDJtS85ZJXVoqmrLGeakESl5XDJiFkWxZhVQcFC9vdNc-JLOxMRmmREkpFVoOxPEN0QS4MOvoVhCvTABnrjdCXBiIgzMejW5rJeeaUqilZIoDR1vaqkKwCNqO1Q431fr5ChuLgwvgd0R3I51bmkX4YSRXVKtR4M1GIIbLHlM2K5cseg8dhv763JXWknM9oK_-Qe-_3YZawHAB17VhqGtHUXMkWa2EFPWoNb2HGr4GV27oJbZu2N9JeLuTMPYbf-UF9CmZ47Ov_8-eft9lX99hlwg-L1Pw_fjK0i4ob0AbQ0oR21uTGTXjyGzdMOPImM3IDGkv7jboNmk7I-IvrTcTJg</recordid><startdate>20141209</startdate><enddate>20141209</enddate><creator>Deng, Bin</creator><creator>Fu, Luoqin</creator><creator>Zhang, Xiaoping</creator><creator>Zheng, Jiajia</creator><creator>Peng, Lisha</creator><creator>Sun, Jiandong</creator><creator>Zhu, Haiyan</creator><creator>Wang, Yibing</creator><creator>Li, Weifen</creator><creator>Wu, Xuexiang</creator><creator>Wu, Di</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141209</creationdate><title>The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture</title><author>Deng, Bin ; Fu, Luoqin ; Zhang, Xiaoping ; Zheng, Jiajia ; Peng, Lisha ; Sun, Jiandong ; Zhu, Haiyan ; Wang, Yibing ; Li, Weifen ; Wu, Xuexiang ; Wu, Di</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9f22bcbd485c99a68e5603b1ed592f1495c03d58eb563e42871a1ca9a68f17e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ammonium</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Aquaculture</topic><topic>Aquaculture industry</topic><topic>Bacillus</topic><topic>Bacteria</topic><topic>Bacterial Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Bin</au><au>Fu, Luoqin</au><au>Zhang, Xiaoping</au><au>Zheng, Jiajia</au><au>Peng, Lisha</au><au>Sun, Jiandong</au><au>Zhu, Haiyan</au><au>Wang, Yibing</au><au>Li, Weifen</au><au>Wu, Xuexiang</au><au>Wu, Di</au><au>Elias, Dwayne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-12-09</date><risdate>2014</risdate><volume>9</volume><issue>12</issue><spage>e114886</spage><epage>e114886</epage><pages>e114886-e114886</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium Pseudomonas stutzeri, SC221-M, was obtained. The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35°C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3× mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24) was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25489740</pmid><doi>10.1371/journal.pone.0114886</doi><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1635000394 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Ammonium Animal sciences Animals Aquaculture Aquaculture industry Bacillus Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biology and Life Sciences Carbon Carbon sources Carp Carps Chemical oxygen demand Citric acid Communities Community structure Ctenopharyngodon idella Denitrification Denitrification - physiology Earth Sciences Ecology and Environmental Sciences Environmental regulations Feed science Feeds Fish Gene Expression Genomes Laboratories Microorganisms Nitrites - metabolism Nitrogen Nitrogen removal Nitrous oxide Nutrition Oxygen Pollutants Pseudomonas Pseudomonas mandelii Pseudomonas stutzeri Pseudomonas stutzeri - isolation & purification Pseudomonas stutzeri - physiology Quality Control Sodium Sodium citrate Sodium nitrite Temperature Temperature range Total dissolved solids Water Purification - methods Water Quality Water quality control Zoology |
| title | The denitrification characteristics of Pseudomonas stutzeri SC221-M and its application to water quality control in grass carp aquaculture |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T08%3A08%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20denitrification%20characteristics%20of%20Pseudomonas%20stutzeri%20SC221-M%20and%20its%20application%20to%20water%20quality%20control%20in%20grass%20carp%20aquaculture&rft.jtitle=PloS%20one&rft.au=Deng,%20Bin&rft.date=2014-12-09&rft.volume=9&rft.issue=12&rft.spage=e114886&rft.epage=e114886&rft.pages=e114886-e114886&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0114886&rft_dat=%3Cgale_plos_%3EA418634389%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1635000394&rft_id=info:pmid/25489740&rft_galeid=A418634389&rft_doaj_id=oai_doaj_org_article_9f864b900a844162a2ec5c77eacea9c0&rfr_iscdi=true |