Functional conservation and divergence of four ginger AP1/AGL9 MADS-box genes revealed by analysis of their expression and protein-protein interaction, and ectopic expression of AhFUL gene in Arabidopsis
Alpinia genus are known generally as ginger-lilies for showy flowers in the ginger family, Zingiberaceae, and their floral morphology diverges from typical monocotyledon flowers. However, little is known about the functions of ginger MADS-box genes in floral identity. In this study, four AP1/AGL9 MA...
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| creator | Li, Xiumei Fan, Tian Song, Juanjuan Sun, Wei Xia, Kuaifei Liao, Jingping Zhang, Mingyong |
| description | Alpinia genus are known generally as ginger-lilies for showy flowers in the ginger family, Zingiberaceae, and their floral morphology diverges from typical monocotyledon flowers. However, little is known about the functions of ginger MADS-box genes in floral identity. In this study, four AP1/AGL9 MADS-box genes were cloned from Alpinia hainanensis, and protein-protein interactions (PPIs) and roles of the four genes in floral homeotic conversion and in floral evolution are surveyed for the first time. AhFUL is clustered to the AP1 lineage, AhSEP4 and AhSEP3b to the SEP lineage, and AhAGL6-like to the AGL6 lineage. The four genes showed conserved and divergent expression patterns, and their encoded proteins were localized in the nucleus. Seven combinations of PPI (AhFUL-AhSEP4, AhFUL-AhAGL6-like, AhFUL-AhSEP3b, AhSEP4-AhAGL6-like, AhSEP4-AhSEP3b, AhAGL6-like-AhSEP3b, and AhSEP3b-AhSEP3b) were detected, and the PPI patterns in the AP1/AGL9 lineage revealed that five of the 10 possible combinations are conserved and three are variable, while conclusions cannot yet be made regarding the other two. Ectopic expression of AhFUL in Arabidopsis thaliana led to early flowering and floral organ homeotic conversion to sepal-like or leaf-like. Therefore, we conclude that the four A. hainanensis AP1/AGL9 genes show functional conservation and divergence in the floral identity from other MADS-box genes. |
| doi_str_mv | 10.1371/journal.pone.0114134 |
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However, little is known about the functions of ginger MADS-box genes in floral identity. In this study, four AP1/AGL9 MADS-box genes were cloned from Alpinia hainanensis, and protein-protein interactions (PPIs) and roles of the four genes in floral homeotic conversion and in floral evolution are surveyed for the first time. AhFUL is clustered to the AP1 lineage, AhSEP4 and AhSEP3b to the SEP lineage, and AhAGL6-like to the AGL6 lineage. The four genes showed conserved and divergent expression patterns, and their encoded proteins were localized in the nucleus. Seven combinations of PPI (AhFUL-AhSEP4, AhFUL-AhAGL6-like, AhFUL-AhSEP3b, AhSEP4-AhAGL6-like, AhSEP4-AhSEP3b, AhAGL6-like-AhSEP3b, and AhSEP3b-AhSEP3b) were detected, and the PPI patterns in the AP1/AGL9 lineage revealed that five of the 10 possible combinations are conserved and three are variable, while conclusions cannot yet be made regarding the other two. Ectopic expression of AhFUL in Arabidopsis thaliana led to early flowering and floral organ homeotic conversion to sepal-like or leaf-like. Therefore, we conclude that the four A. hainanensis AP1/AGL9 genes show functional conservation and divergence in the floral identity from other MADS-box genes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0114134</identifier><identifier>PMID: 25461565</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Arabidopsis thaliana ; Biological evolution ; Biology and Life Sciences ; Botanical gardens ; Conservation ; Conversion ; Divergence ; Ectopic expression ; Evolutionary genetics ; Flowering ; Flowers ; Flowers & plants ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Ginger ; Laboratories ; Morphology ; Oryza ; Phylogeny ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Binding ; Protein interaction ; Protein-protein interactions ; Proteins ; Rice ; Transcription factors ; Zingiber officinale - genetics</subject><ispartof>PloS one, 2014-12, Vol.9 (12), p.e114134-e114134</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Li et al 2014 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-aa5b60d5f755a9de84d09fc2251a01f4b78bc8b4128f94c4db21bf6edea22ad83</citedby><cites>FETCH-LOGICAL-c692t-aa5b60d5f755a9de84d09fc2251a01f4b78bc8b4128f94c4db21bf6edea22ad83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252096/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252096/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25461565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aceto, Serena</contributor><creatorcontrib>Li, Xiumei</creatorcontrib><creatorcontrib>Fan, Tian</creatorcontrib><creatorcontrib>Song, Juanjuan</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Xia, Kuaifei</creatorcontrib><creatorcontrib>Liao, Jingping</creatorcontrib><creatorcontrib>Zhang, Mingyong</creatorcontrib><title>Functional conservation and divergence of four ginger AP1/AGL9 MADS-box genes revealed by analysis of their expression and protein-protein interaction, and ectopic expression of AhFUL gene in Arabidopsis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Alpinia genus are known generally as ginger-lilies for showy flowers in the ginger family, Zingiberaceae, and their floral morphology diverges from typical monocotyledon flowers. However, little is known about the functions of ginger MADS-box genes in floral identity. In this study, four AP1/AGL9 MADS-box genes were cloned from Alpinia hainanensis, and protein-protein interactions (PPIs) and roles of the four genes in floral homeotic conversion and in floral evolution are surveyed for the first time. AhFUL is clustered to the AP1 lineage, AhSEP4 and AhSEP3b to the SEP lineage, and AhAGL6-like to the AGL6 lineage. The four genes showed conserved and divergent expression patterns, and their encoded proteins were localized in the nucleus. Seven combinations of PPI (AhFUL-AhSEP4, AhFUL-AhAGL6-like, AhFUL-AhSEP3b, AhSEP4-AhAGL6-like, AhSEP4-AhSEP3b, AhAGL6-like-AhSEP3b, and AhSEP3b-AhSEP3b) were detected, and the PPI patterns in the AP1/AGL9 lineage revealed that five of the 10 possible combinations are conserved and three are variable, while conclusions cannot yet be made regarding the other two. Ectopic expression of AhFUL in Arabidopsis thaliana led to early flowering and floral organ homeotic conversion to sepal-like or leaf-like. Therefore, we conclude that the four A. hainanensis AP1/AGL9 genes show functional conservation and divergence in the floral identity from other MADS-box genes.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biological evolution</subject><subject>Biology and Life Sciences</subject><subject>Botanical gardens</subject><subject>Conservation</subject><subject>Conversion</subject><subject>Divergence</subject><subject>Ectopic expression</subject><subject>Evolutionary genetics</subject><subject>Flowering</subject><subject>Flowers</subject><subject>Flowers & plants</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Ginger</subject><subject>Laboratories</subject><subject>Morphology</subject><subject>Oryza</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Binding</subject><subject>Protein interaction</subject><subject>Protein-protein interactions</subject><subject>Proteins</subject><subject>Rice</subject><subject>Transcription factors</subject><subject>Zingiber officinale - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9Fu0zAUhiMEYmPwBggsTUIg0S52bCe5QYoGG5OKhhjj1nKck9RTagc7qbZn5KVw2mxq0S5QLhw73___jo9PFL3G8RwnKT65sYMzsp131sA8xpjihD6JDnGekBkncfJ05_0geuH9TRyzJOP8eXRAGOWYcXYY_TkbjOq1DU5IWePBreU4RdJUqNJrcA0YBcjWqA6BqNGmAYeK7_ikOF_k6Fvx-WpW2lsUMPDIwRpkCxUq74KDbO-89qO2X4J2CG47B97f23fO9qDNbBqRNj04udnNxw0AqredVru64FUsz64Xm7ygQIWTpa5sF4JeRs9q2Xp4NY1H0fXZl5-nX2eLy_OL02IxUzwn_UxKVvK4YnXKmMwryGgV57UihGEZ45qWaVaqrKSYZHVOFa1KgsuaQwWSEFllyVH0duvbtdaLqQ5eYE5ylqeUskBcbInKyhvROb2S7k5YqcVmwbpGSNdr1YLAiQppNWdKYRqrRIZd8JKlCU4znOQ4eH2a0oZyBZUC0zvZ7pnufzF6KRq7FpQwEuc8GLyfDJz9PYDvxUp7BW0rDdhh3HdCCc9DVECP_0Ef_7uJakKphTa1DblqNBUFxTxNM4rHU5o_QoWngpUOVw1qHdb3BB_2BIHp4bZv5OC9uLj68f_s5a999t0Ouwz3s1962w7jPfP7IN2CylnvHdQPh4xjMfbc_WmIsefE1HNB9ma3QA-i-yZL_gKduCoy</recordid><startdate>20141202</startdate><enddate>20141202</enddate><creator>Li, Xiumei</creator><creator>Fan, Tian</creator><creator>Song, Juanjuan</creator><creator>Sun, Wei</creator><creator>Xia, Kuaifei</creator><creator>Liao, Jingping</creator><creator>Zhang, Mingyong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141202</creationdate><title>Functional conservation and divergence of four ginger AP1/AGL9 MADS-box genes revealed by analysis of their expression and protein-protein interaction, and ectopic expression of AhFUL gene in Arabidopsis</title><author>Li, Xiumei ; Fan, Tian ; Song, Juanjuan ; Sun, Wei ; Xia, Kuaifei ; Liao, Jingping ; Zhang, Mingyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-aa5b60d5f755a9de84d09fc2251a01f4b78bc8b4128f94c4db21bf6edea22ad83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Biological evolution</topic><topic>Biology and Life Sciences</topic><topic>Botanical gardens</topic><topic>Conservation</topic><topic>Conversion</topic><topic>Divergence</topic><topic>Ectopic expression</topic><topic>Evolutionary genetics</topic><topic>Flowering</topic><topic>Flowers</topic><topic>Flowers & plants</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Ginger</topic><topic>Laboratories</topic><topic>Morphology</topic><topic>Oryza</topic><topic>Phylogeny</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Binding</topic><topic>Protein interaction</topic><topic>Protein-protein interactions</topic><topic>Proteins</topic><topic>Rice</topic><topic>Transcription factors</topic><topic>Zingiber officinale - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiumei</creatorcontrib><creatorcontrib>Fan, Tian</creatorcontrib><creatorcontrib>Song, Juanjuan</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Xia, Kuaifei</creatorcontrib><creatorcontrib>Liao, Jingping</creatorcontrib><creatorcontrib>Zhang, Mingyong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiumei</au><au>Fan, Tian</au><au>Song, Juanjuan</au><au>Sun, Wei</au><au>Xia, Kuaifei</au><au>Liao, Jingping</au><au>Zhang, Mingyong</au><au>Aceto, Serena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional conservation and divergence of four ginger AP1/AGL9 MADS-box genes revealed by analysis of their expression and protein-protein interaction, and ectopic expression of AhFUL gene in Arabidopsis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-12-02</date><risdate>2014</risdate><volume>9</volume><issue>12</issue><spage>e114134</spage><epage>e114134</epage><pages>e114134-e114134</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Alpinia genus are known generally as ginger-lilies for showy flowers in the ginger family, Zingiberaceae, and their floral morphology diverges from typical monocotyledon flowers. However, little is known about the functions of ginger MADS-box genes in floral identity. In this study, four AP1/AGL9 MADS-box genes were cloned from Alpinia hainanensis, and protein-protein interactions (PPIs) and roles of the four genes in floral homeotic conversion and in floral evolution are surveyed for the first time. AhFUL is clustered to the AP1 lineage, AhSEP4 and AhSEP3b to the SEP lineage, and AhAGL6-like to the AGL6 lineage. The four genes showed conserved and divergent expression patterns, and their encoded proteins were localized in the nucleus. Seven combinations of PPI (AhFUL-AhSEP4, AhFUL-AhAGL6-like, AhFUL-AhSEP3b, AhSEP4-AhAGL6-like, AhSEP4-AhSEP3b, AhAGL6-like-AhSEP3b, and AhSEP3b-AhSEP3b) were detected, and the PPI patterns in the AP1/AGL9 lineage revealed that five of the 10 possible combinations are conserved and three are variable, while conclusions cannot yet be made regarding the other two. Ectopic expression of AhFUL in Arabidopsis thaliana led to early flowering and floral organ homeotic conversion to sepal-like or leaf-like. Therefore, we conclude that the four A. hainanensis AP1/AGL9 genes show functional conservation and divergence in the floral identity from other MADS-box genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25461565</pmid><doi>10.1371/journal.pone.0114134</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Arabidopsis Arabidopsis - genetics Arabidopsis thaliana Biological evolution Biology and Life Sciences Botanical gardens Conservation Conversion Divergence Ectopic expression Evolutionary genetics Flowering Flowers Flowers & plants Gene expression Gene Expression Regulation, Plant Genes Genes, Plant Ginger Laboratories Morphology Oryza Phylogeny Plant Proteins - genetics Plant Proteins - metabolism Protein Binding Protein interaction Protein-protein interactions Proteins Rice Transcription factors Zingiber officinale - genetics |
| title | Functional conservation and divergence of four ginger AP1/AGL9 MADS-box genes revealed by analysis of their expression and protein-protein interaction, and ectopic expression of AhFUL gene in Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T22%3A48%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20conservation%20and%20divergence%20of%20four%20ginger%20AP1/AGL9%20MADS-box%20genes%20revealed%20by%20analysis%20of%20their%20expression%20and%20protein-protein%20interaction,%20and%20ectopic%20expression%20of%20AhFUL%20gene%20in%20Arabidopsis&rft.jtitle=PloS%20one&rft.au=Li,%20Xiumei&rft.date=2014-12-02&rft.volume=9&rft.issue=12&rft.spage=e114134&rft.epage=e114134&rft.pages=e114134-e114134&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0114134&rft_dat=%3Cgale_plos_%3EA416778418%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1629597445&rft_id=info:pmid/25461565&rft_galeid=A416778418&rft_doaj_id=oai_doaj_org_article_13cbc8f65cc140c3a51a6b5731781391&rfr_iscdi=true |