Coupled protein diffusion and folding in the cell

When a protein unfolds in the cell, its diffusion coefficient is affected by its increased hydrodynamic radius and by interactions of exposed hydrophobic residues with the cytoplasmic matrix, including chaperones. We characterize protein diffusion by photobleaching whole cells at a single point, and...

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Veröffentlicht in:	PloS one 2014-12, Vol.9 (12), p.e113040-e113040
Hauptverfasser:	Guo, Minghao, Gelman, Hannah, Gruebele, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Biology and Life Sciences Biophysics Cell Line, Tumor Chaperones Clustering Cytosol Cytosol - enzymology Diffusion Diffusion coefficient Diffusion equations Diffusion rate Enzyme Stability Fluorescence Green fluorescent protein HSP70 Heat-Shock Proteins - metabolism Humans Hydrophobicity Lasers Mathematical models Methods Models, Molecular Mutation Phosphoglycerate kinase Phosphoglycerate Kinase - chemistry Phosphoglycerate Kinase - genetics Phosphoglycerate Kinase - metabolism Photobleaching Physics Protein Conformation Protein expression Protein Folding Punctum minutissimum Recovery (Medical) Research and Analysis Methods Scaling Temperature Temperature dependence
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