Comparative salivary proteome of hepatitis B- and C-infected patients
Hepatitis B and C virus (HBV and HCV) infections are an important cause of cirrhosis and hepatocellular carcinoma. The natural history has a prominent latent phase, and infected patients may remain undiagnosed; this situation may lead to the continuing spread of these infections in the community. Co...
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| description | Hepatitis B and C virus (HBV and HCV) infections are an important cause of cirrhosis and hepatocellular carcinoma. The natural history has a prominent latent phase, and infected patients may remain undiagnosed; this situation may lead to the continuing spread of these infections in the community. Compelling reasons exist for using saliva as a diagnostic fluid because it meets the demands of being an inexpensive, noninvasive and easy-to-use diagnostic method. Indeed, comparative analysis of the salivary proteome using mass spectrometry is a promising new strategy for identifying biomarkers. Our goal is to apply an Orbitrap-based quantitative approach to explore the salivary proteome profile in HBV- and HCV-infected patients. In the present study, whole saliva was obtained from 20 healthy, (control) 20 HBV-infected and 20 HCV-infected subjects. Two distinct pools containing saliva from 10 subjects of each group were obtained. The samples were ultracentrifuged and fractionated, and all fractions were hydrolyzed (trypsin) and injected into an LTQ-VELOS ORBITRAP. The identification and analyses of peptides were performed using Proteome Discoverer1.3 and ScaffoldQ + v.3.3.1. From a total of 362 distinct proteins identified, 344 proteins were identified in the HBV, 326 in the HCV and 303 in the control groups. Some blood proteins, such as flavin reductase (which converts biliverdin to bilirubin), were detected only in the HCV group. The data showed a reduced presence of complement C3, ceruloplasmin, alpha(1)-acid glycoprotein and alpha(2)-acid glycoprotein in the hepatitis-infected patients. Peptides of serotransferrin and haptoglobin were less detected in the HCV group. This study provides an integrated perspective of the salivary proteome, which should be further explored in future studies targeting specific disease markers for HBV and HCV infection. |
| doi_str_mv | 10.1371/journal.pone.0113683 |
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The natural history has a prominent latent phase, and infected patients may remain undiagnosed; this situation may lead to the continuing spread of these infections in the community. Compelling reasons exist for using saliva as a diagnostic fluid because it meets the demands of being an inexpensive, noninvasive and easy-to-use diagnostic method. Indeed, comparative analysis of the salivary proteome using mass spectrometry is a promising new strategy for identifying biomarkers. Our goal is to apply an Orbitrap-based quantitative approach to explore the salivary proteome profile in HBV- and HCV-infected patients. In the present study, whole saliva was obtained from 20 healthy, (control) 20 HBV-infected and 20 HCV-infected subjects. Two distinct pools containing saliva from 10 subjects of each group were obtained. The samples were ultracentrifuged and fractionated, and all fractions were hydrolyzed (trypsin) and injected into an LTQ-VELOS ORBITRAP. The identification and analyses of peptides were performed using Proteome Discoverer1.3 and ScaffoldQ + v.3.3.1. From a total of 362 distinct proteins identified, 344 proteins were identified in the HBV, 326 in the HCV and 303 in the control groups. Some blood proteins, such as flavin reductase (which converts biliverdin to bilirubin), were detected only in the HCV group. The data showed a reduced presence of complement C3, ceruloplasmin, alpha(1)-acid glycoprotein and alpha(2)-acid glycoprotein in the hepatitis-infected patients. Peptides of serotransferrin and haptoglobin were less detected in the HCV group. This study provides an integrated perspective of the salivary proteome, which should be further explored in future studies targeting specific disease markers for HBV and HCV infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0113683</identifier><identifier>PMID: 25423034</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Bilirubin ; Biliverdin ; Biochemistry ; Biology and Life Sciences ; Biomarkers ; Ceruloplasmin ; Cirrhosis ; Comparative analysis ; Complement component C3 ; Diagnosis ; Diagnostic systems ; Flavin reductase ; Fluids ; Glycoproteins ; Haptoglobin ; Health aspects ; Hepacivirus - metabolism ; Hepatitis ; Hepatitis B ; Hepatitis B - metabolism ; Hepatitis B virus - metabolism ; Hepatitis C - metabolism ; Hepatitis C virus ; Hepatocellular carcinoma ; Humans ; Immunoassay ; Infection ; Infections ; Laboratories ; Liver cancer ; Liver cirrhosis ; Liver diseases ; Mass spectrometry ; Mass spectroscopy ; Medicine and Health Sciences ; Patients ; Peptides ; Proteins ; Proteome ; Proteomes ; Proteomics ; Saliva ; Saliva - metabolism ; Trypsin ; Viral Proteins - metabolism ; Viruses</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e113683-e113683</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Gonçalves et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Gonçalves et al 2014 Gonçalves et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c762t-1acb0ae42e1f4bd5b035d96b3159a6354f6e5263aea65646bc3b09ef5fdc77863</citedby><cites>FETCH-LOGICAL-c762t-1acb0ae42e1f4bd5b035d96b3159a6354f6e5263aea65646bc3b09ef5fdc77863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244100/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244100/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25423034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ray, Ranjit</contributor><creatorcontrib>Gonçalves, Lorena Da Rós</creatorcontrib><creatorcontrib>Campanhon, Isabele Batista</creatorcontrib><creatorcontrib>Domingues, Romênia R</creatorcontrib><creatorcontrib>Paes Leme, Adriana F</creatorcontrib><creatorcontrib>Soares da Silva, Márcia Regina</creatorcontrib><title>Comparative salivary proteome of hepatitis B- and C-infected patients</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hepatitis B and C virus (HBV and HCV) infections are an important cause of cirrhosis and hepatocellular carcinoma. 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This study provides an integrated perspective of the salivary proteome, which should be further explored in future studies targeting specific disease markers for HBV and HCV infection.</description><subject>Acids</subject><subject>Bilirubin</subject><subject>Biliverdin</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Ceruloplasmin</subject><subject>Cirrhosis</subject><subject>Comparative analysis</subject><subject>Complement component C3</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Flavin reductase</subject><subject>Fluids</subject><subject>Glycoproteins</subject><subject>Haptoglobin</subject><subject>Health aspects</subject><subject>Hepacivirus - metabolism</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B - metabolism</subject><subject>Hepatitis B virus - metabolism</subject><subject>Hepatitis C - metabolism</subject><subject>Hepatitis C virus</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Infection</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Liver cirrhosis</subject><subject>Liver diseases</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine and Health Sciences</subject><subject>Patients</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Saliva</subject><subject>Saliva - metabolism</subject><subject>Trypsin</subject><subject>Viral Proteins - metabolism</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkk1v1DAQhiMEoqXwDxBEQkJwyOJvJxeksiqwUqVKfF0tx5nsukriNHZW8O9xumm1QT0gH2J5nnknM_MmyUuMVphK_OHajUOnm1XvOlghjKnI6aPkFBeUZIIg-vjofpI88_4aIU5zIZ4mJ4QzQhFlp8nF2rW9HnSwe0i9buxeD3_SfnABXAupq9Md9DEarE8_ZanuqnSd2a4GE6BKpwh0wT9PntS68fBi_p4lPz9f_Fh_zS6vvmzW55eZkYKEDGtTIg2MAK5ZWfESUV4VoqSYF1pQzmoBnAiqQQsumCgNLVEBNa8rI2Uu6Fny-qDbN86reQJeYUFkkUuWk0hsDkTl9LXqB9vGfpTTVt0-uGGr9BCsaUCJHCpARVUTzJgkJOcV5SU1EklOZI6i1se52li2UJnY6aCbhegy0tmd2rq9YoQxjCaBd7PA4G5G8EG11htoGt2BG2__O-dxF2zq7M0_6MPdzdRWxwbiGlysayZRdc6wlAWVciq7eoCKp4LWmmiX2sb3RcL7RUJkAvwOWz16rzbfv_0_e_Vryb49Ynegm7DzrhmDdZ1fguwAmsF5P0B9P2SM1OT2u2moye1qdntMe3W8oPukO3vTv7m4-EA</recordid><startdate>20141125</startdate><enddate>20141125</enddate><creator>Gonçalves, Lorena Da Rós</creator><creator>Campanhon, Isabele Batista</creator><creator>Domingues, Romênia R</creator><creator>Paes Leme, Adriana F</creator><creator>Soares da Silva, Márcia Regina</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141125</creationdate><title>Comparative salivary proteome of hepatitis B- and C-infected patients</title><author>Gonçalves, Lorena Da Rós ; Campanhon, Isabele Batista ; Domingues, Romênia R ; Paes Leme, Adriana F ; Soares da Silva, Márcia Regina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c762t-1acb0ae42e1f4bd5b035d96b3159a6354f6e5263aea65646bc3b09ef5fdc77863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acids</topic><topic>Bilirubin</topic><topic>Biliverdin</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Ceruloplasmin</topic><topic>Cirrhosis</topic><topic>Comparative analysis</topic><topic>Complement component C3</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Flavin reductase</topic><topic>Fluids</topic><topic>Glycoproteins</topic><topic>Haptoglobin</topic><topic>Health aspects</topic><topic>Hepacivirus - metabolism</topic><topic>Hepatitis</topic><topic>Hepatitis B</topic><topic>Hepatitis B - metabolism</topic><topic>Hepatitis B virus - metabolism</topic><topic>Hepatitis C - metabolism</topic><topic>Hepatitis C virus</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Infection</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Liver cancer</topic><topic>Liver cirrhosis</topic><topic>Liver diseases</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicine and Health Sciences</topic><topic>Patients</topic><topic>Peptides</topic><topic>Proteins</topic><topic>Proteome</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Saliva</topic><topic>Saliva - metabolism</topic><topic>Trypsin</topic><topic>Viral Proteins - metabolism</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonçalves, Lorena Da Rós</creatorcontrib><creatorcontrib>Campanhon, Isabele Batista</creatorcontrib><creatorcontrib>Domingues, Romênia R</creatorcontrib><creatorcontrib>Paes Leme, Adriana F</creatorcontrib><creatorcontrib>Soares da Silva, Márcia Regina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonçalves, Lorena Da Rós</au><au>Campanhon, Isabele Batista</au><au>Domingues, Romênia R</au><au>Paes Leme, Adriana F</au><au>Soares da Silva, Márcia Regina</au><au>Ray, Ranjit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative salivary proteome of hepatitis B- and C-infected patients</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-11-25</date><risdate>2014</risdate><volume>9</volume><issue>11</issue><spage>e113683</spage><epage>e113683</epage><pages>e113683-e113683</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hepatitis B and C virus (HBV and HCV) infections are an important cause of cirrhosis and hepatocellular carcinoma. The natural history has a prominent latent phase, and infected patients may remain undiagnosed; this situation may lead to the continuing spread of these infections in the community. Compelling reasons exist for using saliva as a diagnostic fluid because it meets the demands of being an inexpensive, noninvasive and easy-to-use diagnostic method. Indeed, comparative analysis of the salivary proteome using mass spectrometry is a promising new strategy for identifying biomarkers. Our goal is to apply an Orbitrap-based quantitative approach to explore the salivary proteome profile in HBV- and HCV-infected patients. In the present study, whole saliva was obtained from 20 healthy, (control) 20 HBV-infected and 20 HCV-infected subjects. Two distinct pools containing saliva from 10 subjects of each group were obtained. The samples were ultracentrifuged and fractionated, and all fractions were hydrolyzed (trypsin) and injected into an LTQ-VELOS ORBITRAP. The identification and analyses of peptides were performed using Proteome Discoverer1.3 and ScaffoldQ + v.3.3.1. From a total of 362 distinct proteins identified, 344 proteins were identified in the HBV, 326 in the HCV and 303 in the control groups. Some blood proteins, such as flavin reductase (which converts biliverdin to bilirubin), were detected only in the HCV group. The data showed a reduced presence of complement C3, ceruloplasmin, alpha(1)-acid glycoprotein and alpha(2)-acid glycoprotein in the hepatitis-infected patients. Peptides of serotransferrin and haptoglobin were less detected in the HCV group. This study provides an integrated perspective of the salivary proteome, which should be further explored in future studies targeting specific disease markers for HBV and HCV infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25423034</pmid><doi>10.1371/journal.pone.0113683</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-11, Vol.9 (11), p.e113683-e113683 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1627987482 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Bilirubin Biliverdin Biochemistry Biology and Life Sciences Biomarkers Ceruloplasmin Cirrhosis Comparative analysis Complement component C3 Diagnosis Diagnostic systems Flavin reductase Fluids Glycoproteins Haptoglobin Health aspects Hepacivirus - metabolism Hepatitis Hepatitis B Hepatitis B - metabolism Hepatitis B virus - metabolism Hepatitis C - metabolism Hepatitis C virus Hepatocellular carcinoma Humans Immunoassay Infection Infections Laboratories Liver cancer Liver cirrhosis Liver diseases Mass spectrometry Mass spectroscopy Medicine and Health Sciences Patients Peptides Proteins Proteome Proteomes Proteomics Saliva Saliva - metabolism Trypsin Viral Proteins - metabolism Viruses |
| title | Comparative salivary proteome of hepatitis B- and C-infected patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T00%3A31%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20salivary%20proteome%20of%20hepatitis%20B-%20and%20C-infected%20patients&rft.jtitle=PloS%20one&rft.au=Gon%C3%A7alves,%20Lorena%20Da%20R%C3%B3s&rft.date=2014-11-25&rft.volume=9&rft.issue=11&rft.spage=e113683&rft.epage=e113683&rft.pages=e113683-e113683&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0113683&rft_dat=%3Cgale_plos_%3EA417793770%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1627987482&rft_id=info:pmid/25423034&rft_galeid=A417793770&rft_doaj_id=oai_doaj_org_article_68ede09df214472285d35b3c70752780&rfr_iscdi=true |