Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation
Vascular endothelial hyperpermeability is one of the manifestations of endothelial dysfunction. Resveratrol (Res) is considered to be beneficial in protecting endothelial function. However, currently, the exact protective effect and involved mechanisms of Res on endothelial dysfunction-hyperpermeabi...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-11, Vol.9 (11), p.e113716-e113716 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e113716 |
|---|---|
| container_issue | 11 |
| container_start_page | e113716 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Peng, Xiao Lin Qu, Wei Wang, Lin Zhi Huang, Bin Qing Ying, Chen Jiang Sun, Xiu Fa Hao, Li Ping |
| description | Vascular endothelial hyperpermeability is one of the manifestations of endothelial dysfunction. Resveratrol (Res) is considered to be beneficial in protecting endothelial function. However, currently, the exact protective effect and involved mechanisms of Res on endothelial dysfunction-hyperpermeability have not been completely clarified. The aim of present study is to investigate the effects of Res on amelioration of endothelial hyperpermeability and the role of caveolin-1 (Cav-1)/endothelial nitric oxide synthase (eNOS) pathway. Adult male Wistar rats were treated with a normal or high-fat/sucrose diet (HFS) with or without Res for 13 weeks. HFS and in vitro treatment with high glucose increased hyperpermeability in rat aorta, heart, liver and kidney and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Application of Res reversed the changes in eNOS and Cav-1 expressions in aorta and heart of rats fed HFS and in BAECs incubated with high glucose. Res stimulated the formation of NO inhibited by high glucose in BAECs. Beta-Cyclodextrin (β-CD), caveolae inhibitor, showed the better beneficial effect than Res alone to up-regulate eNOS phosphorylative levels, while NG-Nitro-77 L-arginine methyl ester (L-NAME), eNOS inhibitor, had no effect on Cav-1 expression. Our studies suggested that HFS and in vitro treatment with high glucose caused endothelial hyperpermeability, which were ameliorated by Res at least involving Cav-1/eNOS regulation. |
| doi_str_mv | 10.1371/journal.pone.0113716 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1627710914</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A417844093</galeid><doaj_id>oai_doaj_org_article_b320f2a179704da28e8a5550611e6d8e</doaj_id><sourcerecordid>A417844093</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-a198187d8a4803b72aa15f85327b2847d3b122392243be7a96c5c379392cc03a3</originalsourceid><addsrcrecordid>eNqNk01r3DAQhk1padK0_6C0hkJpD97oy5Z8KYTQj0BoIGl7FWN57FXQWhvJXppj_3nlZBOyJYdige1Xz7ySRjNZ9pqSBeWSHl76KQzgFms_4ILQWaueZPu05qyoGOFPH3zvZS9ivCSk5Kqqnmd7rBS0rqXYz_6cY9xggDF4l8MKnfXpB2O-tP0y791kfMQchvZGKDoYD-Nkwiy2FsfCDu1ksM03EM3kIOTL6zWGNFYIjXV2vM7tsPFuY4c-P4ZNQQ_x-9lFHrBP-Gj98DJ71oGL-Gr7Psh-fvn84_hbcXr29eT46LQwslRjAbRWVMlWgVCEN5IB0LJTJWeyYUrIljeUMV4zJniDEurKlIbLOinGEA78IHt767t2Pupt9qKmFZOSkpqKRJzcEq2HS70OdgXhWnuw-kbwodcQRmsc6oYz0jGgspZEtMAUKijLklSUYtUqTF6ftqtNzQpbg8MYwO2Y7s4Mdql7v9GCCSZZmQw-bA2Cv5owjnplo0HnYEA_3exbMUEpqxL67h_08dNtqR7SAezQ-bSumU31kaBSCUFqnqjFI1R6WlxZk0qts0nfCfi4E5CYEX-PPUwx6pOL8_9nz37tsu8fsEsENy6jd9NcMnEXFLfgXJUxYHefZEr03BV32dBzp-htp6SwNw8v6D7orjX4X3OHDns</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1627710914</pqid></control><display><type>article</type><title>Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation</title><source>MEDLINE</source><source>Free E-Journal (出版社公開部分のみ）</source><source>PLoS - Public Library of Sciencem (Open Access)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>DOAJ: Directory of Open Access Journals</source><creator>Peng, Xiao Lin ; Qu, Wei ; Wang, Lin Zhi ; Huang, Bin Qing ; Ying, Chen Jiang ; Sun, Xiu Fa ; Hao, Li Ping</creator><creatorcontrib>Peng, Xiao Lin ; Qu, Wei ; Wang, Lin Zhi ; Huang, Bin Qing ; Ying, Chen Jiang ; Sun, Xiu Fa ; Hao, Li Ping</creatorcontrib><description>Vascular endothelial hyperpermeability is one of the manifestations of endothelial dysfunction. Resveratrol (Res) is considered to be beneficial in protecting endothelial function. However, currently, the exact protective effect and involved mechanisms of Res on endothelial dysfunction-hyperpermeability have not been completely clarified. The aim of present study is to investigate the effects of Res on amelioration of endothelial hyperpermeability and the role of caveolin-1 (Cav-1)/endothelial nitric oxide synthase (eNOS) pathway. Adult male Wistar rats were treated with a normal or high-fat/sucrose diet (HFS) with or without Res for 13 weeks. HFS and in vitro treatment with high glucose increased hyperpermeability in rat aorta, heart, liver and kidney and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Application of Res reversed the changes in eNOS and Cav-1 expressions in aorta and heart of rats fed HFS and in BAECs incubated with high glucose. Res stimulated the formation of NO inhibited by high glucose in BAECs. Beta-Cyclodextrin (β-CD), caveolae inhibitor, showed the better beneficial effect than Res alone to up-regulate eNOS phosphorylative levels, while NG-Nitro-77 L-arginine methyl ester (L-NAME), eNOS inhibitor, had no effect on Cav-1 expression. Our studies suggested that HFS and in vitro treatment with high glucose caused endothelial hyperpermeability, which were ameliorated by Res at least involving Cav-1/eNOS regulation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0113716</identifier><identifier>PMID: 25419974</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis Inhibitors - pharmacology ; Animals ; Aorta ; Arginine ; Biology and Life Sciences ; Blotting, Western ; Capillary Permeability - drug effects ; Cattle ; Caveolae ; Caveolin ; Caveolin 1 - genetics ; Caveolin 1 - metabolism ; Caveolin-1 ; Cells, Cultured ; Cyclodextrins ; Diet ; Diet, High-Fat ; Dietary Carbohydrates ; Dietary Fats - administration & dosage ; Endothelial cells ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Gene Expression Regulation - drug effects ; Glucose ; Glucose - administration & dosage ; Heart ; Inhibitors ; Kidneys ; Kinases ; Liver ; Male ; Medicine and Health Sciences ; NG-Nitroarginine methyl ester ; Nitric oxide ; Nitric Oxide Synthase Type III - genetics ; Nitric Oxide Synthase Type III - metabolism ; Nitric-oxide synthase ; Nutrition ; Phosphorylation - drug effects ; Polyphenols ; Rats ; Rats, Wistar ; Resveratrol ; Reverse Transcriptase Polymerase Chain Reaction ; Rodents ; Stilbenes - pharmacology ; Sucrose ; Sucrose - administration & dosage ; Sugar ; β-Cyclodextrin</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e113716-e113716</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Peng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Peng et al 2014 Peng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-a198187d8a4803b72aa15f85327b2847d3b122392243be7a96c5c379392cc03a3</citedby><cites>FETCH-LOGICAL-c758t-a198187d8a4803b72aa15f85327b2847d3b122392243be7a96c5c379392cc03a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242725/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242725/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25419974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Xiao Lin</creatorcontrib><creatorcontrib>Qu, Wei</creatorcontrib><creatorcontrib>Wang, Lin Zhi</creatorcontrib><creatorcontrib>Huang, Bin Qing</creatorcontrib><creatorcontrib>Ying, Chen Jiang</creatorcontrib><creatorcontrib>Sun, Xiu Fa</creatorcontrib><creatorcontrib>Hao, Li Ping</creatorcontrib><title>Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Vascular endothelial hyperpermeability is one of the manifestations of endothelial dysfunction. Resveratrol (Res) is considered to be beneficial in protecting endothelial function. However, currently, the exact protective effect and involved mechanisms of Res on endothelial dysfunction-hyperpermeability have not been completely clarified. The aim of present study is to investigate the effects of Res on amelioration of endothelial hyperpermeability and the role of caveolin-1 (Cav-1)/endothelial nitric oxide synthase (eNOS) pathway. Adult male Wistar rats were treated with a normal or high-fat/sucrose diet (HFS) with or without Res for 13 weeks. HFS and in vitro treatment with high glucose increased hyperpermeability in rat aorta, heart, liver and kidney and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Application of Res reversed the changes in eNOS and Cav-1 expressions in aorta and heart of rats fed HFS and in BAECs incubated with high glucose. Res stimulated the formation of NO inhibited by high glucose in BAECs. Beta-Cyclodextrin (β-CD), caveolae inhibitor, showed the better beneficial effect than Res alone to up-regulate eNOS phosphorylative levels, while NG-Nitro-77 L-arginine methyl ester (L-NAME), eNOS inhibitor, had no effect on Cav-1 expression. Our studies suggested that HFS and in vitro treatment with high glucose caused endothelial hyperpermeability, which were ameliorated by Res at least involving Cav-1/eNOS regulation.</description><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Aorta</subject><subject>Arginine</subject><subject>Biology and Life Sciences</subject><subject>Blotting, Western</subject><subject>Capillary Permeability - drug effects</subject><subject>Cattle</subject><subject>Caveolae</subject><subject>Caveolin</subject><subject>Caveolin 1 - genetics</subject><subject>Caveolin 1 - metabolism</subject><subject>Caveolin-1</subject><subject>Cells, Cultured</subject><subject>Cyclodextrins</subject><subject>Diet</subject><subject>Diet, High-Fat</subject><subject>Dietary Carbohydrates</subject><subject>Dietary Fats - administration & dosage</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glucose</subject><subject>Glucose - administration & dosage</subject><subject>Heart</subject><subject>Inhibitors</subject><subject>Kidneys</subject><subject>Kinases</subject><subject>Liver</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>NG-Nitroarginine methyl ester</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type III - genetics</subject><subject>Nitric Oxide Synthase Type III - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Nutrition</subject><subject>Phosphorylation - drug effects</subject><subject>Polyphenols</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Resveratrol</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rodents</subject><subject>Stilbenes - pharmacology</subject><subject>Sucrose</subject><subject>Sucrose - administration & dosage</subject><subject>Sugar</subject><subject>β-Cyclodextrin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01r3DAQhk1padK0_6C0hkJpD97oy5Z8KYTQj0BoIGl7FWN57FXQWhvJXppj_3nlZBOyJYdige1Xz7ySRjNZ9pqSBeWSHl76KQzgFms_4ILQWaueZPu05qyoGOFPH3zvZS9ivCSk5Kqqnmd7rBS0rqXYz_6cY9xggDF4l8MKnfXpB2O-tP0y791kfMQchvZGKDoYD-Nkwiy2FsfCDu1ksM03EM3kIOTL6zWGNFYIjXV2vM7tsPFuY4c-P4ZNQQ_x-9lFHrBP-Gj98DJ71oGL-Gr7Psh-fvn84_hbcXr29eT46LQwslRjAbRWVMlWgVCEN5IB0LJTJWeyYUrIljeUMV4zJniDEurKlIbLOinGEA78IHt767t2Pupt9qKmFZOSkpqKRJzcEq2HS70OdgXhWnuw-kbwodcQRmsc6oYz0jGgspZEtMAUKijLklSUYtUqTF6ftqtNzQpbg8MYwO2Y7s4Mdql7v9GCCSZZmQw-bA2Cv5owjnplo0HnYEA_3exbMUEpqxL67h_08dNtqR7SAezQ-bSumU31kaBSCUFqnqjFI1R6WlxZk0qts0nfCfi4E5CYEX-PPUwx6pOL8_9nz37tsu8fsEsENy6jd9NcMnEXFLfgXJUxYHefZEr03BV32dBzp-htp6SwNw8v6D7orjX4X3OHDns</recordid><startdate>20141124</startdate><enddate>20141124</enddate><creator>Peng, Xiao Lin</creator><creator>Qu, Wei</creator><creator>Wang, Lin Zhi</creator><creator>Huang, Bin Qing</creator><creator>Ying, Chen Jiang</creator><creator>Sun, Xiu Fa</creator><creator>Hao, Li Ping</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141124</creationdate><title>Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation</title><author>Peng, Xiao Lin ; Qu, Wei ; Wang, Lin Zhi ; Huang, Bin Qing ; Ying, Chen Jiang ; Sun, Xiu Fa ; Hao, Li Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-a198187d8a4803b72aa15f85327b2847d3b122392243be7a96c5c379392cc03a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Aorta</topic><topic>Arginine</topic><topic>Biology and Life Sciences</topic><topic>Blotting, Western</topic><topic>Capillary Permeability - drug effects</topic><topic>Cattle</topic><topic>Caveolae</topic><topic>Caveolin</topic><topic>Caveolin 1 - genetics</topic><topic>Caveolin 1 - metabolism</topic><topic>Caveolin-1</topic><topic>Cells, Cultured</topic><topic>Cyclodextrins</topic><topic>Diet</topic><topic>Diet, High-Fat</topic><topic>Dietary Carbohydrates</topic><topic>Dietary Fats - administration & dosage</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glucose</topic><topic>Glucose - administration & dosage</topic><topic>Heart</topic><topic>Inhibitors</topic><topic>Kidneys</topic><topic>Kinases</topic><topic>Liver</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>NG-Nitroarginine methyl ester</topic><topic>Nitric oxide</topic><topic>Nitric Oxide Synthase Type III - genetics</topic><topic>Nitric Oxide Synthase Type III - metabolism</topic><topic>Nitric-oxide synthase</topic><topic>Nutrition</topic><topic>Phosphorylation - drug effects</topic><topic>Polyphenols</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Resveratrol</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rodents</topic><topic>Stilbenes - pharmacology</topic><topic>Sucrose</topic><topic>Sucrose - administration & dosage</topic><topic>Sugar</topic><topic>β-Cyclodextrin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Xiao Lin</creatorcontrib><creatorcontrib>Qu, Wei</creatorcontrib><creatorcontrib>Wang, Lin Zhi</creatorcontrib><creatorcontrib>Huang, Bin Qing</creatorcontrib><creatorcontrib>Ying, Chen Jiang</creatorcontrib><creatorcontrib>Sun, Xiu Fa</creatorcontrib><creatorcontrib>Hao, Li Ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ: Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Xiao Lin</au><au>Qu, Wei</au><au>Wang, Lin Zhi</au><au>Huang, Bin Qing</au><au>Ying, Chen Jiang</au><au>Sun, Xiu Fa</au><au>Hao, Li Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-11-24</date><risdate>2014</risdate><volume>9</volume><issue>11</issue><spage>e113716</spage><epage>e113716</epage><pages>e113716-e113716</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Vascular endothelial hyperpermeability is one of the manifestations of endothelial dysfunction. Resveratrol (Res) is considered to be beneficial in protecting endothelial function. However, currently, the exact protective effect and involved mechanisms of Res on endothelial dysfunction-hyperpermeability have not been completely clarified. The aim of present study is to investigate the effects of Res on amelioration of endothelial hyperpermeability and the role of caveolin-1 (Cav-1)/endothelial nitric oxide synthase (eNOS) pathway. Adult male Wistar rats were treated with a normal or high-fat/sucrose diet (HFS) with or without Res for 13 weeks. HFS and in vitro treatment with high glucose increased hyperpermeability in rat aorta, heart, liver and kidney and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Application of Res reversed the changes in eNOS and Cav-1 expressions in aorta and heart of rats fed HFS and in BAECs incubated with high glucose. Res stimulated the formation of NO inhibited by high glucose in BAECs. Beta-Cyclodextrin (β-CD), caveolae inhibitor, showed the better beneficial effect than Res alone to up-regulate eNOS phosphorylative levels, while NG-Nitro-77 L-arginine methyl ester (L-NAME), eNOS inhibitor, had no effect on Cav-1 expression. Our studies suggested that HFS and in vitro treatment with high glucose caused endothelial hyperpermeability, which were ameliorated by Res at least involving Cav-1/eNOS regulation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25419974</pmid><doi>10.1371/journal.pone.0113716</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-11, Vol.9 (11), p.e113716-e113716 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1627710914 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; PLoS - Public Library of Sciencem (Open Access); PubMed Central; Free Full-Text Journals in Chemistry; DOAJ: Directory of Open Access Journals |
| subjects | Angiogenesis Inhibitors - pharmacology Animals Aorta Arginine Biology and Life Sciences Blotting, Western Capillary Permeability - drug effects Cattle Caveolae Caveolin Caveolin 1 - genetics Caveolin 1 - metabolism Caveolin-1 Cells, Cultured Cyclodextrins Diet Diet, High-Fat Dietary Carbohydrates Dietary Fats - administration & dosage Endothelial cells Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Gene Expression Regulation - drug effects Glucose Glucose - administration & dosage Heart Inhibitors Kidneys Kinases Liver Male Medicine and Health Sciences NG-Nitroarginine methyl ester Nitric oxide Nitric Oxide Synthase Type III - genetics Nitric Oxide Synthase Type III - metabolism Nitric-oxide synthase Nutrition Phosphorylation - drug effects Polyphenols Rats Rats, Wistar Resveratrol Reverse Transcriptase Polymerase Chain Reaction Rodents Stilbenes - pharmacology Sucrose Sucrose - administration & dosage Sugar β-Cyclodextrin |
| title | Resveratrol ameliorates high glucose and high-fat/sucrose diet-induced vascular hyperpermeability involving Cav-1/eNOS regulation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T15%3A31%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Resveratrol%20ameliorates%20high%20glucose%20and%20high-fat/sucrose%20diet-induced%20vascular%20hyperpermeability%20involving%20Cav-1/eNOS%20regulation&rft.jtitle=PloS%20one&rft.au=Peng,%20Xiao%20Lin&rft.date=2014-11-24&rft.volume=9&rft.issue=11&rft.spage=e113716&rft.epage=e113716&rft.pages=e113716-e113716&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0113716&rft_dat=%3Cgale_plos_%3EA417844093%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1627710914&rft_id=info:pmid/25419974&rft_galeid=A417844093&rft_doaj_id=oai_doaj_org_article_b320f2a179704da28e8a5550611e6d8e&rfr_iscdi=true |